Reviewed, UniProtKB/Swiss-Prot Q08686 (THTR_YEAST)

Names and origin

Protein names

Recommended name:
    Putative thiosulfate sulfurtransferase
    EC=2.8.1.1

Gene names

Ordered Locus Names:

YOR251C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Thiosulfate + cyanide = sulfite + thiocyanate.
Subcellular location	Mitochondrion. Cytoplasm.
Miscellaneous	Present with 5770 molecules/cell in log phase SD medium.
Sequence similarities	Contains 2 rhodanese domains.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion
Domain	Repeat
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate transport Inferred from electronic annotation. Source: InterPro tRNA wobble base modification Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct thiosulfate sulfurtransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AUS1	Q08409	1	EBI-19264,EBI-35723
RSP5	P39940	1	EBI-19264,EBI-16219
SSM4	P40318	1	EBI-19264,EBI-18208

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Putative thiosulfate sulfurtransferase		PRO_0000139402
Regions
	Domain	20 – 137	118	Rhodanese 1
	Domain	177 – 299	123	Rhodanese 2
Sites
	Active site	259	1	Cysteine persulfide intermediate By similarity
	Binding site	191	1	Substrate By similarity
Amino acid modifications
	Modified residue	264	1	Phosphoserine

Sequences

	Sequence		Length	Mass (Da)
	Q08686-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F8082DF7CC279E82	FASTA	304	34,219
	10 20 30 40 50 60 MPLFDLISPK AFVKLVASEK VHRIVPVDAT WYLPSWKLDN KVDFLTKPRI PNSIFFDIDA 70 80 90 100 110 120 ISDKKSPYPH MFPTKKVFDD AMSNLGVQKD DILVVYDRVG NFSSPRCAWT LGVMGHPKVY 130 140 150 160 170 180 LLNNFNQYRE FKYPLDSSKV AAFSPYPKSH YESSESFQDK EIVDYEEMFQ LVKSGELAKK 190 200 210 220 230 240 FNAFDARSLG RFEGTEPEPR SDIPSGHIPG TQPLPYGSLL DPETKTYPEA GEAIHATLEK 250 260 270 280 290 300 ALKDFHCTLD PSKPTICSCG TGVSGVIIKT ALELAGVPNV RLYDGSWTEW VLKSGPEWIA ENRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing analysis of a 36.8 kb fragment of yeast chromosome XV reveals 26 open reading frames including SEC63, CDC31, SUG2, GCD1, RBL2, PNT1, PAC1 and VPH1." Poirey R., Jauniaux J.-C. Yeast 13:483-487(1997) [PubMed: 9153759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-264, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z75159 Genomic DNA. Translation: CAA99473.1. AY692732 Genomic DNA. Translation: AAT92751.1.
PIR	S67148.
RefSeq	NP_014894.1.
3D structure databases
HSSP	HSSP built from PDB template 1RHS based on UniProtKB P00586.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4457N.
IntAct	Q08686.
Proteomic databases
PeptideAtlas	Q08686.
Genome annotation databases
Ensembl	YOR251C. Saccharomyces cerevisiae. [Contig view]
GeneID	854425.
GenomeReviews	Gene locus YOR251C in contig Y13140_GR.
KEGG	sce:YOR251C.
NMPDR	fig|4932.3.peg.6006.
Organism-specific databases
CYGD	YOR251c.
SGD	S000005777. YOR251C.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q08686.
Gene expression databases
ArrayExpress	Q08686.
GermOnline	YOR251C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001763. Rhodanese-like. IPR001307. Thiosulphate_sulphurtransf_CS. [Graphical view]
Gene3D	G3DSA:3.40.250.10. Rhodanese-like. 2 hits.
Pfam	PF00581. Rhodanese. 1 hit. [Graphical view]
SMART	SM00450. RHOD. 2 hits. [Graphical view]
PROSITE	PS00683. RHODANESE_2. 1 hit. PS50206. RHODANESE_3. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	Q08686.
NextBio	976640.

Entry information

Entry name

THTR_YEAST

Accession

Primary (citable) accession number: Q08686

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)