Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thiosulfate sulfurtransferase TUM1

Gene

TUM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for formation of the 2-thio group of the 5-methoxycarbonylmethyl-2-thiouridine modified base in some tRNAs.1 Publication

Miscellaneous

Present with 5770 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Thiosulfate + cyanide = sulfite + thiocyanate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei191SubstrateBy similarity1
Active sitei259Cysteine persulfide intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • thiosulfate sulfurtransferase activity Source: SGD

GO - Biological processi

  • tRNA wobble position uridine thiolation Source: SGD
  • tRNA wobble uridine modification Source: SGD

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BioCyciMetaCyc:YOR251C-MONOMER
YEAST:YOR251C-MONOMER
ReactomeiR-SCE-1614558 Degradation of cysteine and homocysteine

Names & Taxonomyi

Protein namesi
Recommended name:
Thiosulfate sulfurtransferase TUM1 (EC:2.8.1.1)
Alternative name(s):
Thiouridine modification protein 1
Gene namesi
Name:TUM1
Ordered Locus Names:YOR251C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR251C
SGDiS000005777 TUM1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001394021 – 304Thiosulfate sulfurtransferase TUM1Add BLAST304

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei201PhosphoserineCombined sources1
Modified residuei264PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08686
PaxDbiQ08686
PRIDEiQ08686

PTM databases

iPTMnetiQ08686

Interactioni

Protein-protein interaction databases

BioGridi34641, 100 interactors
DIPiDIP-4457N
IntActiQ08686, 9 interactors
STRINGi4932.YOR251C

Structurei

Secondary structure

1304
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 7Combined sources3
Helixi9 – 18Combined sources10
Beta strandi20 – 22Combined sources3
Beta strandi24 – 28Combined sources5
Helixi34 – 36Combined sources3
Helixi40 – 46Combined sources7
Turni58 – 60Combined sources3
Beta strandi66 – 68Combined sources3
Helixi75 – 84Combined sources10
Beta strandi92 – 96Combined sources5
Beta strandi98 – 103Combined sources6
Helixi104 – 113Combined sources10
Beta strandi117 – 123Combined sources7
Helixi125 – 130Combined sources6
Helixi158 – 161Combined sources4
Helixi165 – 173Combined sources9
Helixi177 – 180Combined sources4
Beta strandi182 – 185Combined sources4
Helixi189 – 192Combined sources4
Beta strandi199 – 202Combined sources4
Beta strandi211 – 213Combined sources3
Helixi216 – 219Combined sources4
Turni222 – 224Combined sources3
Helixi232 – 244Combined sources13
Beta strandi255 – 258Combined sources4
Beta strandi260 – 262Combined sources3
Helixi263 – 274Combined sources12
Beta strandi279 – 285Combined sources7
Helixi286 – 294Combined sources9
Helixi296 – 298Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UTNX-ray1.90X1-304[»]
ProteinModelPortaliQ08686
SMRiQ08686
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 137Rhodanese 1PROSITE-ProRule annotationAdd BLAST118
Domaini177 – 299Rhodanese 2PROSITE-ProRule annotationAdd BLAST123

Domaini

Contains two rhodanese domains with different primary structures but with near identical secondary structure conformations suggesting a common evolutionary origin. Only the C-terminal rhodanese domain contains the catalytic cysteine residue (By similarity).By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00510000046773
HOGENOMiHOG000157237
InParanoidiQ08686
KOiK01011
OMAiCGKPDVP
OrthoDBiEOG092C4KLI

Family and domain databases

Gene3Di3.40.250.10, 2 hits
InterProiView protein in InterPro
IPR001763 Rhodanese-like_dom
IPR036873 Rhodanese-like_dom_sf
IPR001307 Thiosulphate_STrfase_CS
PfamiView protein in Pfam
PF00581 Rhodanese, 2 hits
SMARTiView protein in SMART
SM00450 RHOD, 2 hits
SUPFAMiSSF52821 SSF52821, 2 hits
PROSITEiView protein in PROSITE
PS00683 RHODANESE_2, 1 hit
PS50206 RHODANESE_3, 2 hits

Sequencei

Sequence statusi: Complete.

Q08686-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLFDLISPK AFVKLVASEK VHRIVPVDAT WYLPSWKLDN KVDFLTKPRI
60 70 80 90 100
PNSIFFDIDA ISDKKSPYPH MFPTKKVFDD AMSNLGVQKD DILVVYDRVG
110 120 130 140 150
NFSSPRCAWT LGVMGHPKVY LLNNFNQYRE FKYPLDSSKV AAFSPYPKSH
160 170 180 190 200
YESSESFQDK EIVDYEEMFQ LVKSGELAKK FNAFDARSLG RFEGTEPEPR
210 220 230 240 250
SDIPSGHIPG TQPLPYGSLL DPETKTYPEA GEAIHATLEK ALKDFHCTLD
260 270 280 290 300
PSKPTICSCG TGVSGVIIKT ALELAGVPNV RLYDGSWTEW VLKSGPEWIA

ENRD
Length:304
Mass (Da):34,219
Last modified:November 1, 1996 - v1
Checksum:iF8082DF7CC279E82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75159 Genomic DNA Translation: CAA99473.1
AY692732 Genomic DNA Translation: AAT92751.1
BK006948 Genomic DNA Translation: DAA11018.1
PIRiS67148
RefSeqiNP_014894.3, NM_001183670.3

Genome annotation databases

EnsemblFungiiYOR251C; YOR251C; YOR251C
GeneIDi854425
KEGGisce:YOR251C

Similar proteinsi

Entry informationi

Entry nameiTHTR_YEAST
AccessioniPrimary (citable) accession number: Q08686
Secondary accession number(s): D6W2V2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health