Q08686 (THTR_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiosulfate sulfurtransferase TUM1
EC=2.8.1.1

Alternative name(s):
Thiouridine modification protein 1

Gene names

Name:	TUM1
Ordered Locus Names:	YOR251C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for formation of the 2-thio group of the 5-methoxycarbonylmethyl-2-thiouridine modified base in some tRNAs. Ref.8
Catalytic activity	Thiosulfate + cyanide = sulfite + thiocyanate.
Subcellular location	Mitochondrion. Cytoplasm Ref.5.
Miscellaneous	Present with 5770 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Contains 2 rhodanese domains.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion
Domain	Repeat
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tRNA wobble position uridine thiolation Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB thiosulfate sulfurtransferase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AUS1	Q08409	1	EBI-19264,EBI-35723
RSP5	P39940	1	EBI-19264,EBI-16219
SSM4	P40318	1	EBI-19264,EBI-18208

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Thiosulfate sulfurtransferase TUM1		PRO_0000139402
Regions
	Domain	20 – 137	118	Rhodanese 1
	Domain	177 – 299	123	Rhodanese 2
Sites
	Active site	259	1	Cysteine persulfide intermediate By similarity
	Binding site	191	1	Substrate By similarity
Amino acid modifications
	Modified residue	264	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q08686 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F8082DF7CC279E82	FASTA	304	34,219
	10 20 30 40 50 60 MPLFDLISPK AFVKLVASEK VHRIVPVDAT WYLPSWKLDN KVDFLTKPRI PNSIFFDIDA 70 80 90 100 110 120 ISDKKSPYPH MFPTKKVFDD AMSNLGVQKD DILVVYDRVG NFSSPRCAWT LGVMGHPKVY 130 140 150 160 170 180 LLNNFNQYRE FKYPLDSSKV AAFSPYPKSH YESSESFQDK EIVDYEEMFQ LVKSGELAKK 190 200 210 220 230 240 FNAFDARSLG RFEGTEPEPR SDIPSGHIPG TQPLPYGSLL DPETKTYPEA GEAIHATLEK 250 260 270 280 290 300 ALKDFHCTLD PSKPTICSCG TGVSGVIIKT ALELAGVPNV RLYDGSWTEW VLKSGPEWIA ENRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing analysis of a 36.8 kb fragment of yeast chromosome XV reveals 26 open reading frames including SEC63, CDC31, SUG2, GCD1, RBL2, PNT1, PAC1 and VPH1." Poirey R., Jauniaux J.-C. Yeast 13:483-487(1997) [PubMed: 9153759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-264, MASS SPECTROMETRY.
[8]	"A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae." Huang B., Lu J., Bystroem A.S. RNA 14:2183-2194(2008) [PubMed: 18755837] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z75159 Genomic DNA. Translation: CAA99473.1. AY692732 Genomic DNA. Translation: AAT92751.1. BK006948 Genomic DNA. Translation: DAA11018.1.
PIR	S67148.
RefSeq	NP_014894.1. NM_001183670.1.
3D structure databases
ProteinModelPortal	Q08686.
SMR	Q08686. Positions 4-297.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4457N.
IntAct	Q08686. 6 interactions.
MINT	MINT-478300.
STRING	Q08686.
Proteomic databases
PeptideAtlas	Q08686.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR251C; YOR251C; YOR251C.
GeneID	854425.
KEGG	sce:YOR251C.
NMPDR	fig|4932.3.peg.6006.
Organism-specific databases
CYGD	YOR251c.
SGD	S000005777. TUM1.
Phylogenomic databases
eggNOG	fuNOG05123.
GeneTree	EFGT00050000006151.
HOGENOM	HBG709927.
OMA	GSWVEWY.
OrthoDB	EOG4F7RV7.
Gene expression databases
ArrayExpress	Q08686.
Genevestigator	Q08686.
GermOnline	YOR251C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001763. Rhodanese-like. IPR001307. Thiosulphate_STrfase_CS. [Graphical view]
Gene3D	G3DSA:3.40.250.10. Rhodanese-like. 2 hits.
KO	K01011.
Pfam	PF00581. Rhodanese. 2 hits. [Graphical view]
SMART	SM00450. RHOD. 2 hits. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 2 hits.
PROSITE	PS00683. RHODANESE_2. 1 hit. PS50206. RHODANESE_3. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	976640.

Entry information

Entry name

THTR_YEAST

Accession

Primary (citable) accession number: Q08686
Secondary accession number(s): D6W2V2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program