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Protein

Thiosulfate sulfurtransferase TUM1

Gene

TUM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for formation of the 2-thio group of the 5-methoxycarbonylmethyl-2-thiouridine modified base in some tRNAs.1 Publication

Catalytic activityi

Thiosulfate + cyanide = sulfite + thiocyanate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei191SubstrateBy similarity1
Active sitei259Cysteine persulfide intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • thiosulfate sulfurtransferase activity Source: SGD

GO - Biological processi

  • tRNA wobble position uridine thiolation Source: SGD
  • tRNA wobble uridine modification Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciYEAST:YOR251C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiosulfate sulfurtransferase TUM1 (EC:2.8.1.1)
Alternative name(s):
Thiouridine modification protein 1
Gene namesi
Name:TUM1
Ordered Locus Names:YOR251C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR251C.
SGDiS000005777. TUM1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001394021 – 304Thiosulfate sulfurtransferase TUM1Add BLAST304

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei201PhosphoserineCombined sources1
Modified residuei264PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08686.
PRIDEiQ08686.

PTM databases

iPTMnetiQ08686.

Interactioni

Protein-protein interaction databases

BioGridi34641. 38 interactors.
DIPiDIP-4457N.
IntActiQ08686. 6 interactors.
MINTiMINT-478300.

Structurei

Secondary structure

1304
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 7Combined sources3
Helixi9 – 18Combined sources10
Beta strandi20 – 22Combined sources3
Beta strandi24 – 28Combined sources5
Helixi34 – 36Combined sources3
Helixi40 – 46Combined sources7
Turni58 – 60Combined sources3
Beta strandi66 – 68Combined sources3
Helixi75 – 84Combined sources10
Beta strandi92 – 96Combined sources5
Beta strandi98 – 103Combined sources6
Helixi104 – 113Combined sources10
Beta strandi117 – 123Combined sources7
Helixi125 – 130Combined sources6
Helixi158 – 161Combined sources4
Helixi165 – 173Combined sources9
Helixi177 – 180Combined sources4
Beta strandi182 – 185Combined sources4
Helixi189 – 192Combined sources4
Beta strandi199 – 202Combined sources4
Beta strandi211 – 213Combined sources3
Helixi216 – 219Combined sources4
Turni222 – 224Combined sources3
Helixi232 – 244Combined sources13
Beta strandi255 – 258Combined sources4
Beta strandi260 – 262Combined sources3
Helixi263 – 274Combined sources12
Beta strandi279 – 285Combined sources7
Helixi286 – 294Combined sources9
Helixi296 – 298Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UTNX-ray1.90X1-304[»]
ProteinModelPortaliQ08686.
SMRiQ08686.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 137Rhodanese 1PROSITE-ProRule annotationAdd BLAST118
Domaini177 – 299Rhodanese 2PROSITE-ProRule annotationAdd BLAST123

Domaini

Contains two rhodanese domains with different primary structures but with near identical secondary structure conformations suggesting a common evolutionary origin. Only the C-terminal rhodanese domain contains the catalytic cysteine residue (By similarity).By similarity

Sequence similaritiesi

Contains 2 rhodanese domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00510000046773.
HOGENOMiHOG000157237.
InParanoidiQ08686.
KOiK01011.
OMAiAWFLPND.
OrthoDBiEOG092C4KLI.

Family and domain databases

Gene3Di3.40.250.10. 2 hits.
InterProiIPR001763. Rhodanese-like_dom.
IPR001307. Thiosulphate_STrfase_CS.
[Graphical view]
PfamiPF00581. Rhodanese. 2 hits.
[Graphical view]
SMARTiSM00450. RHOD. 2 hits.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 2 hits.
PROSITEiPS00683. RHODANESE_2. 1 hit.
PS50206. RHODANESE_3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08686-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLFDLISPK AFVKLVASEK VHRIVPVDAT WYLPSWKLDN KVDFLTKPRI
60 70 80 90 100
PNSIFFDIDA ISDKKSPYPH MFPTKKVFDD AMSNLGVQKD DILVVYDRVG
110 120 130 140 150
NFSSPRCAWT LGVMGHPKVY LLNNFNQYRE FKYPLDSSKV AAFSPYPKSH
160 170 180 190 200
YESSESFQDK EIVDYEEMFQ LVKSGELAKK FNAFDARSLG RFEGTEPEPR
210 220 230 240 250
SDIPSGHIPG TQPLPYGSLL DPETKTYPEA GEAIHATLEK ALKDFHCTLD
260 270 280 290 300
PSKPTICSCG TGVSGVIIKT ALELAGVPNV RLYDGSWTEW VLKSGPEWIA

ENRD
Length:304
Mass (Da):34,219
Last modified:November 1, 1996 - v1
Checksum:iF8082DF7CC279E82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75159 Genomic DNA. Translation: CAA99473.1.
AY692732 Genomic DNA. Translation: AAT92751.1.
BK006948 Genomic DNA. Translation: DAA11018.1.
PIRiS67148.
RefSeqiNP_014894.3. NM_001183670.3.

Genome annotation databases

EnsemblFungiiYOR251C; YOR251C; YOR251C.
GeneIDi854425.
KEGGisce:YOR251C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75159 Genomic DNA. Translation: CAA99473.1.
AY692732 Genomic DNA. Translation: AAT92751.1.
BK006948 Genomic DNA. Translation: DAA11018.1.
PIRiS67148.
RefSeqiNP_014894.3. NM_001183670.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UTNX-ray1.90X1-304[»]
ProteinModelPortaliQ08686.
SMRiQ08686.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34641. 38 interactors.
DIPiDIP-4457N.
IntActiQ08686. 6 interactors.
MINTiMINT-478300.

PTM databases

iPTMnetiQ08686.

Proteomic databases

MaxQBiQ08686.
PRIDEiQ08686.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR251C; YOR251C; YOR251C.
GeneIDi854425.
KEGGisce:YOR251C.

Organism-specific databases

EuPathDBiFungiDB:YOR251C.
SGDiS000005777. TUM1.

Phylogenomic databases

GeneTreeiENSGT00510000046773.
HOGENOMiHOG000157237.
InParanoidiQ08686.
KOiK01011.
OMAiAWFLPND.
OrthoDBiEOG092C4KLI.

Enzyme and pathway databases

BioCyciYEAST:YOR251C-MONOMER.

Miscellaneous databases

PROiQ08686.

Family and domain databases

Gene3Di3.40.250.10. 2 hits.
InterProiIPR001763. Rhodanese-like_dom.
IPR001307. Thiosulphate_STrfase_CS.
[Graphical view]
PfamiPF00581. Rhodanese. 2 hits.
[Graphical view]
SMARTiSM00450. RHOD. 2 hits.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 2 hits.
PROSITEiPS00683. RHODANESE_2. 1 hit.
PS50206. RHODANESE_3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHTR_YEAST
AccessioniPrimary (citable) accession number: Q08686
Secondary accession number(s): D6W2V2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5770 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.