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Q08682

- RSSA1_ARATH

UniProt

Q08682 - RSSA1_ARATH

Protein

40S ribosomal protein Sa-1

Gene

RPSaA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 3 (30 Aug 2005)
      Previous versions | rss
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    Functioni

    Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.UniRule annotation

    GO - Molecular functioni

    1. structural constituent of ribosome Source: RefGenome

    GO - Biological processi

    1. endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: RefGenome
    2. endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: RefGenome
    3. response to osmotic stress Source: TAIR
    4. response to salt stress Source: TAIR
    5. ribosomal small subunit assembly Source: RefGenome
    6. rRNA export from nucleus Source: RefGenome
    7. translation Source: RefGenome

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    ReactomeiREACT_187631. SRP-dependent cotranslational protein targeting to membrane.
    REACT_187829. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_190931. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    40S ribosomal protein Sa-1
    Alternative name(s):
    Laminin receptor homolog
    p40
    Gene namesi
    Name:RPSaA
    Ordered Locus Names:At1g72370
    ORF Names:T10D10.16
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G72370.

    Subcellular locationi

    GO - Cellular componenti

    1. 90S preribosome Source: RefGenome
    2. chloroplast Source: TAIR
    3. cytoplasm Source: TAIR
    4. cytosol Source: TAIR
    5. cytosolic ribosome Source: TAIR
    6. cytosolic small ribosomal subunit Source: TAIR
    7. membrane Source: TAIR
    8. nucleus Source: TAIR
    9. plasma membrane Source: TAIR
    10. plasmodesma Source: TAIR
    11. small ribosomal subunit Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 PublicationUniRule annotation
    Chaini2 – 29829740S ribosomal protein Sa-1PRO_0000134346Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ08682.
    PRIDEiQ08682.

    Expressioni

    Gene expression databases

    ArrayExpressiQ08682.
    GenevestigatoriQ08682.

    Interactioni

    Subunit structurei

    Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with ribosomal protein S21.UniRule annotation

    Protein-protein interaction databases

    BioGridi28789. 5 interactions.
    IntActiQ08682. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08682.
    SMRiQ08682. Positions 12-197.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi247 – 29852Ala/Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ribosomal protein S2P family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0052.
    HOGENOMiHOG000232073.
    InParanoidiQ08682.
    KOiK02998.
    OMAiPIMEASY.
    PhylomeDBiQ08682.

    Family and domain databases

    HAMAPiMF_03015. Ribosomal_S2_euk.
    InterProiIPR001865. Ribosomal_S2.
    IPR018130. Ribosomal_S2_CS.
    IPR027498. Ribosomal_S2_euk.
    IPR005707. Ribosomal_S2_euk/arc.
    IPR023591. Ribosomal_S2_flav_dom.
    [Graphical view]
    PANTHERiPTHR11489. PTHR11489. 1 hit.
    PfamiPF00318. Ribosomal_S2. 2 hits.
    [Graphical view]
    PRINTSiPR00395. RIBOSOMALS2.
    SUPFAMiSSF52313. SSF52313. 1 hit.
    TIGRFAMsiTIGR01012. Sa_S2_E_A. 1 hit.
    PROSITEiPS00962. RIBOSOMAL_S2_1. 1 hit.
    PS00963. RIBOSOMAL_S2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q08682-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MATNGSASSA QLSQKEADVR MMCAAEVHLG TKNCNYQMER YVFKRRNDGI    50
    YIFNLGKTWE KLQMAARVIV AIENPQDIIV QSARPYGQRA VLKFAQYTGA 100
    NAIAGRHTPG TFTNQMQTSF SEPRLLILTD PRTDHQPIKE GALGNIPIIA 150
    FCDTDSPMRF VDIGIPANNK GKHSIGCLFW LLARMVLQMR GTIAAGQKWD 200
    VMVDLFFYRE PEETKPEDED EAGPQAEYGA LPAPEYGMVG GDQWTTAQIP 250
    DAAWPGEGQA PISAAPAAAS WSDSAAAPAD GGWEAAAPPS GAPAAGWE 298
    Length:298
    Mass (Da):32,291
    Last modified:August 30, 2005 - v3
    Checksum:iE5C1A846A30161B6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti11 – 122QL → HV(PubMed:8208855)Curated
    Sequence conflicti11 – 122QL → HV1 PublicationCurated
    Sequence conflicti11 – 122QL → HV(PubMed:9426609)Curated
    Sequence conflicti211 – 2111P → L in CAA48794. (PubMed:8208855)Curated
    Sequence conflicti212 – 2132EE → DY1 PublicationCurated
    Sequence conflicti212 – 2132EE → DY(PubMed:9426609)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69056 mRNA. Translation: CAA48794.1.
    U01955 mRNA. Translation: AAA53425.1.
    X89366 Genomic DNA. Translation: CAA61547.1.
    Y10379 Genomic DNA. Translation: CAA71407.1.
    AC016529 Genomic DNA. Translation: AAG52587.1.
    CP002684 Genomic DNA. Translation: AEE35313.1.
    AY054211 mRNA. Translation: AAL06872.1.
    AY058885 mRNA. Translation: AAL24271.1.
    AY065096 mRNA. Translation: AAL38272.1.
    AY079041 mRNA. Translation: AAL79591.1.
    AY114561 mRNA. Translation: AAM47880.1.
    AY136324 mRNA. Translation: AAM96990.1.
    BT000421 mRNA. Translation: AAN15740.1.
    AY087976 mRNA. Translation: AAM65523.1.
    PIRiF96747.
    S71247.
    RefSeqiNP_177381.1. NM_105896.3. [Q08682-1]
    UniGeneiAt.22576.
    At.35111.
    At.70162.

    Genome annotation databases

    EnsemblPlantsiAT1G72370.1; AT1G72370.1; AT1G72370. [Q08682-1]
    GeneIDi843569.
    KEGGiath:AT1G72370.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69056 mRNA. Translation: CAA48794.1 .
    U01955 mRNA. Translation: AAA53425.1 .
    X89366 Genomic DNA. Translation: CAA61547.1 .
    Y10379 Genomic DNA. Translation: CAA71407.1 .
    AC016529 Genomic DNA. Translation: AAG52587.1 .
    CP002684 Genomic DNA. Translation: AEE35313.1 .
    AY054211 mRNA. Translation: AAL06872.1 .
    AY058885 mRNA. Translation: AAL24271.1 .
    AY065096 mRNA. Translation: AAL38272.1 .
    AY079041 mRNA. Translation: AAL79591.1 .
    AY114561 mRNA. Translation: AAM47880.1 .
    AY136324 mRNA. Translation: AAM96990.1 .
    BT000421 mRNA. Translation: AAN15740.1 .
    AY087976 mRNA. Translation: AAM65523.1 .
    PIRi F96747.
    S71247.
    RefSeqi NP_177381.1. NM_105896.3. [Q08682-1 ]
    UniGenei At.22576.
    At.35111.
    At.70162.

    3D structure databases

    ProteinModelPortali Q08682.
    SMRi Q08682. Positions 12-197.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 28789. 5 interactions.
    IntActi Q08682. 2 interactions.

    Proteomic databases

    PaxDbi Q08682.
    PRIDEi Q08682.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G72370.1 ; AT1G72370.1 ; AT1G72370 . [Q08682-1 ]
    GeneIDi 843569.
    KEGGi ath:AT1G72370.

    Organism-specific databases

    GeneFarmi 5044.
    TAIRi AT1G72370.

    Phylogenomic databases

    eggNOGi COG0052.
    HOGENOMi HOG000232073.
    InParanoidi Q08682.
    KOi K02998.
    OMAi PIMEASY.
    PhylomeDBi Q08682.

    Enzyme and pathway databases

    Reactomei REACT_187631. SRP-dependent cotranslational protein targeting to membrane.
    REACT_187829. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_190931. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).

    Gene expression databases

    ArrayExpressi Q08682.
    Genevestigatori Q08682.

    Family and domain databases

    HAMAPi MF_03015. Ribosomal_S2_euk.
    InterProi IPR001865. Ribosomal_S2.
    IPR018130. Ribosomal_S2_CS.
    IPR027498. Ribosomal_S2_euk.
    IPR005707. Ribosomal_S2_euk/arc.
    IPR023591. Ribosomal_S2_flav_dom.
    [Graphical view ]
    PANTHERi PTHR11489. PTHR11489. 1 hit.
    Pfami PF00318. Ribosomal_S2. 2 hits.
    [Graphical view ]
    PRINTSi PR00395. RIBOSOMALS2.
    SUPFAMi SSF52313. SSF52313. 1 hit.
    TIGRFAMsi TIGR01012. Sa_S2_E_A. 1 hit.
    PROSITEi PS00962. RIBOSOMAL_S2_1. 1 hit.
    PS00963. RIBOSOMAL_S2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An Arabidopsis cDNA encoding a 33-kilodalton laminin receptor homolog."
      Axelos M., Bardet C., Lescure B.
      Plant Physiol. 103:299-300(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Arabidopsis p40 homologue. A novel acidic protein associated with the 40 S subunit of ribosomes."
      Garcia-Hernandez M., Davies E., Staswick P.E.
      J. Biol. Chem. 269:20744-20749(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
      Tissue: Leaf.
    3. "Characterization of a ribosomal p40 homologue gene showing the same pattern of expression as the elongation factor-1 alpha in Arabidopsis thaliana."
      Scheer I., Axelos M., Pont-Lezica R.F.
      Plant Physiol. Biochem. 34:501-508(1996)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
    4. "Expression of a gene encoding a ribosomal p40 protein and identification of an active promoter site."
      Scheer I., Ludevid M.D., Regad F.F., Lescure B., Pont-Lezica R.F.
      Plant Mol. Biol. 35:905-913(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
    5. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    6. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    7. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    8. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    9. "The organization of cytoplasmic ribosomal protein genes in the Arabidopsis genome."
      Barakat A., Szick-Miranda K., Chang I.-F., Guyot R., Blanc G., Cooke R., Delseny M., Bailey-Serres J.
      Plant Physiol. 127:398-415(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
    10. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRSSA1_ARATH
    AccessioniPrimary (citable) accession number: Q08682
    Secondary accession number(s): Q93V81
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 113 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought to be a laminin receptor.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3