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Q08682 (RSSA1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
40S ribosomal protein Sa-1
Alternative name(s):
Laminin receptor homolog
p40
Gene names
Name:RPSaA
Ordered Locus Names:At1g72370
ORF Names:T10D10.16
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length298 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits By similarity. HAMAP-Rule MF_03015

Subunit structure

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with ribosomal protein S21 By similarity.

Subcellular location

Cytoplasm HAMAP-Rule MF_03015.

Sequence similarities

Belongs to the ribosomal protein S2P family.

Caution

Was originally thought to be a laminin receptor.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   Molecular functionRibonucleoprotein
Ribosomal protein
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processendonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

Inferred from Biological aspect of Ancestor. Source: RefGenome

endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

Inferred from Biological aspect of Ancestor. Source: RefGenome

rRNA export from nucleus

Inferred from Biological aspect of Ancestor. Source: RefGenome

response to osmotic stress

Inferred from expression pattern PubMed 16254930. Source: TAIR

response to salt stress

Inferred from expression pattern PubMed 16254930. Source: TAIR

ribosomal small subunit assembly

Inferred from Biological aspect of Ancestor. Source: RefGenome

translation

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_component90S preribosome

Inferred from Biological aspect of Ancestor. Source: RefGenome

chloroplast

Inferred from direct assay PubMed 15028209PubMed 18431481. Source: TAIR

cytoplasm

Inferred from direct assay PubMed 15610358Ref.2. Source: TAIR

cytosol

Inferred from direct assay PubMed 21166475. Source: TAIR

cytosolic ribosome

Inferred from direct assay PubMed 15734919PubMed 15821981. Source: TAIR

cytosolic small ribosomal subunit

Inferred from direct assay PubMed 17934214. Source: TAIR

membrane

Inferred from direct assay PubMed 17432890. Source: TAIR

nucleus

Inferred from direct assay PubMed 14617066PubMed 15610358. Source: TAIR

plasma membrane

Inferred from direct assay PubMed 17317660. Source: TAIR

plasmodesma

Inferred from direct assay PubMed 21533090. Source: TAIR

small ribosomal subunit

Inferred from direct assay Ref.2. Source: TAIR

   Molecular_functionstructural constituent of ribosome

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q08682-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.10
Chain2 – 29829740S ribosomal protein Sa-1 HAMAP-Rule MF_03015
PRO_0000134346

Regions

Compositional bias247 – 29852Ala/Pro-rich HAMAP-Rule MF_03015

Amino acid modifications

Modified residue21N-acetylalanine Ref.10

Experimental info

Sequence conflict11 – 122QL → HV Ref.1
Sequence conflict11 – 122QL → HV Ref.3
Sequence conflict11 – 122QL → HV Ref.4
Sequence conflict2111P → L in CAA48794. Ref.1
Sequence conflict212 – 2132EE → DY Ref.3
Sequence conflict212 – 2132EE → DY Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 30, 2005. Version 3.
Checksum: E5C1A846A30161B6

FASTA29832,291
        10         20         30         40         50         60 
MATNGSASSA QLSQKEADVR MMCAAEVHLG TKNCNYQMER YVFKRRNDGI YIFNLGKTWE 

        70         80         90        100        110        120 
KLQMAARVIV AIENPQDIIV QSARPYGQRA VLKFAQYTGA NAIAGRHTPG TFTNQMQTSF 

       130        140        150        160        170        180 
SEPRLLILTD PRTDHQPIKE GALGNIPIIA FCDTDSPMRF VDIGIPANNK GKHSIGCLFW 

       190        200        210        220        230        240 
LLARMVLQMR GTIAAGQKWD VMVDLFFYRE PEETKPEDED EAGPQAEYGA LPAPEYGMVG 

       250        260        270        280        290 
GDQWTTAQIP DAAWPGEGQA PISAAPAAAS WSDSAAAPAD GGWEAAAPPS GAPAAGWE 

« Hide

References

« Hide 'large scale' references
[1]"An Arabidopsis cDNA encoding a 33-kilodalton laminin receptor homolog."
Axelos M., Bardet C., Lescure B.
Plant Physiol. 103:299-300(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Arabidopsis p40 homologue. A novel acidic protein associated with the 40 S subunit of ribosomes."
Garcia-Hernandez M., Davies E., Staswick P.E.
J. Biol. Chem. 269:20744-20749(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
Tissue: Leaf.
[3]"Characterization of a ribosomal p40 homologue gene showing the same pattern of expression as the elongation factor-1 alpha in Arabidopsis thaliana."
Scheer I., Axelos M., Pont-Lezica R.F.
Plant Physiol. Biochem. 34:501-508(1996)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[4]"Expression of a gene encoding a ribosomal p40 protein and identification of an active promoter site."
Scheer I., Ludevid M.D., Regad F.F., Lescure B., Pont-Lezica R.F.
Plant Mol. Biol. 35:905-913(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[5]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[6]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[7]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[8]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[9]"The organization of cytoplasmic ribosomal protein genes in the Arabidopsis genome."
Barakat A., Szick-Miranda K., Chang I.-F., Guyot R., Blanc G., Cooke R., Delseny M., Bailey-Serres J.
Plant Physiol. 127:398-415(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
[10]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69056 mRNA. Translation: CAA48794.1.
U01955 mRNA. Translation: AAA53425.1.
X89366 Genomic DNA. Translation: CAA61547.1.
Y10379 Genomic DNA. Translation: CAA71407.1.
AC016529 Genomic DNA. Translation: AAG52587.1.
CP002684 Genomic DNA. Translation: AEE35313.1.
AY054211 mRNA. Translation: AAL06872.1.
AY058885 mRNA. Translation: AAL24271.1.
AY065096 mRNA. Translation: AAL38272.1.
AY079041 mRNA. Translation: AAL79591.1.
AY114561 mRNA. Translation: AAM47880.1.
AY136324 mRNA. Translation: AAM96990.1.
BT000421 mRNA. Translation: AAN15740.1.
AY087976 mRNA. Translation: AAM65523.1.
PIRF96747.
S71247.
RefSeqNP_177381.1. NM_105896.3.
UniGeneAt.22576.
At.35111.
At.70162.

3D structure databases

ProteinModelPortalQ08682.
SMRQ08682. Positions 12-211.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid28789. 5 interactions.
IntActQ08682. 2 interactions.

Proteomic databases

PaxDbQ08682.
PRIDEQ08682.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G72370.1; AT1G72370.1; AT1G72370. [Q08682-1]
GeneID843569.
KEGGath:AT1G72370.

Organism-specific databases

GeneFarm5044.
TAIRAT1G72370.

Phylogenomic databases

eggNOGCOG0052.
HOGENOMHOG000232073.
InParanoidQ08682.
KOK02998.
OMAPIMEASY.
PhylomeDBQ08682.
ProtClustDBCLSN2912707.

Gene expression databases

ArrayExpressQ08682.
GenevestigatorQ08682.

Family and domain databases

HAMAPMF_03015. Ribosomal_S2_euk.
InterProIPR001865. Ribosomal_S2.
IPR018130. Ribosomal_S2_CS.
IPR027498. Ribosomal_S2_euk.
IPR005707. Ribosomal_S2_euk/arc.
IPR023591. Ribosomal_S2_flav_dom.
[Graphical view]
PANTHERPTHR11489. PTHR11489. 1 hit.
PfamPF00318. Ribosomal_S2. 2 hits.
[Graphical view]
PRINTSPR00395. RIBOSOMALS2.
SUPFAMSSF52313. SSF52313. 1 hit.
TIGRFAMsTIGR01012. Sa_S2_E_A. 1 hit.
PROSITEPS00962. RIBOSOMAL_S2_1. 1 hit.
PS00963. RIBOSOMAL_S2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRSSA1_ARATH
AccessionPrimary (citable) accession number: Q08682
Secondary accession number(s): Q93V81
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: August 30, 2005
Last modified: April 16, 2014
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names