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Protein

40S ribosomal protein Sa-1

Gene

RPSaA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.UniRule annotation

GO - Molecular functioni

  1. structural constituent of ribosome Source: GO_Central

GO - Biological processi

  1. endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
  2. endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
  3. response to osmotic stress Source: TAIR
  4. response to salt stress Source: TAIR
  5. ribosomal small subunit assembly Source: GO_Central
  6. rRNA export from nucleus Source: GO_Central
  7. translation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

ReactomeiREACT_187631. SRP-dependent cotranslational protein targeting to membrane.
REACT_187829. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_190931. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_232663. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_233701. Ribosomal scanning and start codon recognition.
REACT_238761. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_240283. Peptide chain elongation.
REACT_244451. Formation of the ternary complex, and subsequently, the 43S complex.
REACT_248654. Eukaryotic Translation Termination.
REACT_262398. Formation of a pool of free 40S subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein Sa-1
Alternative name(s):
Laminin receptor homolog
p40
Gene namesi
Name:RPSaA
Ordered Locus Names:At1g72370
ORF Names:T10D10.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G72370.

Subcellular locationi

GO - Cellular componenti

  1. 90S preribosome Source: GO_Central
  2. chloroplast Source: TAIR
  3. cytoplasm Source: TAIR
  4. cytosol Source: TAIR
  5. cytosolic ribosome Source: TAIR
  6. cytosolic small ribosomal subunit Source: TAIR
  7. membrane Source: TAIR
  8. nucleus Source: TAIR
  9. plasma membrane Source: TAIR
  10. plasmodesma Source: TAIR
  11. small ribosomal subunit Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 PublicationUniRule annotation
Chaini2 – 29829740S ribosomal protein Sa-1PRO_0000134346Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ08682.
PRIDEiQ08682.

Expressioni

Gene expression databases

ExpressionAtlasiQ08682. baseline and differential.
GenevestigatoriQ08682.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with ribosomal protein S21.UniRule annotation

Protein-protein interaction databases

BioGridi28789. 5 interactions.
IntActiQ08682. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ08682.
SMRiQ08682. Positions 12-197.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi247 – 29852Ala/Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the ribosomal protein S2P family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0052.
HOGENOMiHOG000232073.
InParanoidiQ08682.
KOiK02998.
OMAiTTHIGST.
PhylomeDBiQ08682.

Family and domain databases

HAMAPiMF_03015. Ribosomal_S2_euk.
InterProiIPR001865. Ribosomal_S2.
IPR018130. Ribosomal_S2_CS.
IPR027498. Ribosomal_S2_euk.
IPR005707. Ribosomal_S2_euk/arc.
IPR023591. Ribosomal_S2_flav_dom.
[Graphical view]
PANTHERiPTHR11489. PTHR11489. 1 hit.
PfamiPF00318. Ribosomal_S2. 2 hits.
[Graphical view]
PRINTSiPR00395. RIBOSOMALS2.
SUPFAMiSSF52313. SSF52313. 1 hit.
TIGRFAMsiTIGR01012. Sa_S2_E_A. 1 hit.
PROSITEiPS00962. RIBOSOMAL_S2_1. 1 hit.
PS00963. RIBOSOMAL_S2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q08682-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATNGSASSA QLSQKEADVR MMCAAEVHLG TKNCNYQMER YVFKRRNDGI
60 70 80 90 100
YIFNLGKTWE KLQMAARVIV AIENPQDIIV QSARPYGQRA VLKFAQYTGA
110 120 130 140 150
NAIAGRHTPG TFTNQMQTSF SEPRLLILTD PRTDHQPIKE GALGNIPIIA
160 170 180 190 200
FCDTDSPMRF VDIGIPANNK GKHSIGCLFW LLARMVLQMR GTIAAGQKWD
210 220 230 240 250
VMVDLFFYRE PEETKPEDED EAGPQAEYGA LPAPEYGMVG GDQWTTAQIP
260 270 280 290
DAAWPGEGQA PISAAPAAAS WSDSAAAPAD GGWEAAAPPS GAPAAGWE
Length:298
Mass (Da):32,291
Last modified:August 30, 2005 - v3
Checksum:iE5C1A846A30161B6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 122QL → HV(PubMed:8208855)Curated
Sequence conflicti11 – 122QL → HV1 PublicationCurated
Sequence conflicti11 – 122QL → HV(PubMed:9426609)Curated
Sequence conflicti211 – 2111P → L in CAA48794. (PubMed:8208855)Curated
Sequence conflicti212 – 2132EE → DY1 PublicationCurated
Sequence conflicti212 – 2132EE → DY(PubMed:9426609)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69056 mRNA. Translation: CAA48794.1.
U01955 mRNA. Translation: AAA53425.1.
X89366 Genomic DNA. Translation: CAA61547.1.
Y10379 Genomic DNA. Translation: CAA71407.1.
AC016529 Genomic DNA. Translation: AAG52587.1.
CP002684 Genomic DNA. Translation: AEE35313.1.
AY054211 mRNA. Translation: AAL06872.1.
AY058885 mRNA. Translation: AAL24271.1.
AY065096 mRNA. Translation: AAL38272.1.
AY079041 mRNA. Translation: AAL79591.1.
AY114561 mRNA. Translation: AAM47880.1.
AY136324 mRNA. Translation: AAM96990.1.
BT000421 mRNA. Translation: AAN15740.1.
AY087976 mRNA. Translation: AAM65523.1.
PIRiF96747.
S71247.
RefSeqiNP_177381.1. NM_105896.3. [Q08682-1]
UniGeneiAt.22576.
At.35111.
At.70162.

Genome annotation databases

EnsemblPlantsiAT1G72370.1; AT1G72370.1; AT1G72370. [Q08682-1]
GeneIDi843569.
KEGGiath:AT1G72370.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69056 mRNA. Translation: CAA48794.1.
U01955 mRNA. Translation: AAA53425.1.
X89366 Genomic DNA. Translation: CAA61547.1.
Y10379 Genomic DNA. Translation: CAA71407.1.
AC016529 Genomic DNA. Translation: AAG52587.1.
CP002684 Genomic DNA. Translation: AEE35313.1.
AY054211 mRNA. Translation: AAL06872.1.
AY058885 mRNA. Translation: AAL24271.1.
AY065096 mRNA. Translation: AAL38272.1.
AY079041 mRNA. Translation: AAL79591.1.
AY114561 mRNA. Translation: AAM47880.1.
AY136324 mRNA. Translation: AAM96990.1.
BT000421 mRNA. Translation: AAN15740.1.
AY087976 mRNA. Translation: AAM65523.1.
PIRiF96747.
S71247.
RefSeqiNP_177381.1. NM_105896.3. [Q08682-1]
UniGeneiAt.22576.
At.35111.
At.70162.

3D structure databases

ProteinModelPortaliQ08682.
SMRiQ08682. Positions 12-197.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28789. 5 interactions.
IntActiQ08682. 2 interactions.

Proteomic databases

PaxDbiQ08682.
PRIDEiQ08682.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G72370.1; AT1G72370.1; AT1G72370. [Q08682-1]
GeneIDi843569.
KEGGiath:AT1G72370.

Organism-specific databases

GeneFarmi5044.
TAIRiAT1G72370.

Phylogenomic databases

eggNOGiCOG0052.
HOGENOMiHOG000232073.
InParanoidiQ08682.
KOiK02998.
OMAiTTHIGST.
PhylomeDBiQ08682.

Enzyme and pathway databases

ReactomeiREACT_187631. SRP-dependent cotranslational protein targeting to membrane.
REACT_187829. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_190931. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_232663. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_233701. Ribosomal scanning and start codon recognition.
REACT_238761. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_240283. Peptide chain elongation.
REACT_244451. Formation of the ternary complex, and subsequently, the 43S complex.
REACT_248654. Eukaryotic Translation Termination.
REACT_262398. Formation of a pool of free 40S subunits.

Gene expression databases

ExpressionAtlasiQ08682. baseline and differential.
GenevestigatoriQ08682.

Family and domain databases

HAMAPiMF_03015. Ribosomal_S2_euk.
InterProiIPR001865. Ribosomal_S2.
IPR018130. Ribosomal_S2_CS.
IPR027498. Ribosomal_S2_euk.
IPR005707. Ribosomal_S2_euk/arc.
IPR023591. Ribosomal_S2_flav_dom.
[Graphical view]
PANTHERiPTHR11489. PTHR11489. 1 hit.
PfamiPF00318. Ribosomal_S2. 2 hits.
[Graphical view]
PRINTSiPR00395. RIBOSOMALS2.
SUPFAMiSSF52313. SSF52313. 1 hit.
TIGRFAMsiTIGR01012. Sa_S2_E_A. 1 hit.
PROSITEiPS00962. RIBOSOMAL_S2_1. 1 hit.
PS00963. RIBOSOMAL_S2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "An Arabidopsis cDNA encoding a 33-kilodalton laminin receptor homolog."
    Axelos M., Bardet C., Lescure B.
    Plant Physiol. 103:299-300(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Arabidopsis p40 homologue. A novel acidic protein associated with the 40 S subunit of ribosomes."
    Garcia-Hernandez M., Davies E., Staswick P.E.
    J. Biol. Chem. 269:20744-20749(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Leaf.
  3. "Characterization of a ribosomal p40 homologue gene showing the same pattern of expression as the elongation factor-1 alpha in Arabidopsis thaliana."
    Scheer I., Axelos M., Pont-Lezica R.F.
    Plant Physiol. Biochem. 34:501-508(1996)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  4. "Expression of a gene encoding a ribosomal p40 protein and identification of an active promoter site."
    Scheer I., Ludevid M.D., Regad F.F., Lescure B., Pont-Lezica R.F.
    Plant Mol. Biol. 35:905-913(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  5. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  6. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  7. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  8. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  9. "The organization of cytoplasmic ribosomal protein genes in the Arabidopsis genome."
    Barakat A., Szick-Miranda K., Chang I.-F., Guyot R., Blanc G., Cooke R., Delseny M., Bailey-Serres J.
    Plant Physiol. 127:398-415(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
  10. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRSSA1_ARATH
AccessioniPrimary (citable) accession number: Q08682
Secondary accession number(s): Q93V81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: August 30, 2005
Last modified: February 4, 2015
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a laminin receptor.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.