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Protein

Anthranilate synthase component 1

Gene

trpE

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia (By similarity).By similarity

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Enzyme regulationi

Feedback inhibited by tryptophan.By similarity

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Bifunctional protein TrpGD (trpGD), Anthranilate synthase component 1 (trpE)
  2. Anthranilate phosphoribosyltransferase (trpD), Bifunctional protein TrpGD (trpGD)
  3. N-(5'-phosphoribosyl)anthranilate isomerase (trpF), N-(5'-phosphoribosyl)anthranilate isomerase (trpF)
  4. Indole-3-glycerol phosphate synthase (trpC), Indole-3-glycerol phosphate synthase (trpC)
  5. Tryptophan synthase beta chain (trpB), Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain 2 (trpB2), Tryptophan synthase beta chain 1 (trpB1), Tryptophan synthase alpha chain (trpA)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36TryptophanBy similarity1
Metal bindingi296MagnesiumBy similarity1
Binding sitei384ChorismateBy similarity1
Binding sitei409ChorismateBy similarity1
Binding sitei425Chorismate; via amide nitrogenBy similarity1
Metal bindingi438MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-281.
UniPathwayiUPA00035; UER00040.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component 1 (EC:4.1.3.27)
Short name:
AS
Short name:
ASI
Gene namesi
Name:trpE
Ordered Locus Names:TM_0142
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001541161 – 461Anthranilate synthase component 1Add BLAST461

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).By similarity

Protein-protein interaction databases

STRINGi243274.TM0142.

Structurei

3D structure databases

ProteinModelPortaliQ08653.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni234 – 236Tryptophan bindingBy similarity3
Regioni269 – 270Chorismate bindingBy similarity2
Regioni423 – 425Chorismate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CRQ. Bacteria.
COG0147. LUCA.
InParanoidiQ08653.
KOiK01657.
OMAiTIRTLEM.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR015890. Chorismate_C.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.

Sequencei

Sequence statusi: Complete.

Q08653-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDSMHVLKL VSDLETPVST FMKVSRGEEF AFLLESVELG SAFGRHSFIG
60 70 80 90 100
IGKKDVLVFE KGILRTSNQQ LDYTSSPLKA IKDWLEVYRY SVKHDELPSF
110 120 130 140 150
RGGAVGFVSY DYISYIEKVK VKASVFPTFY FVVPEHLIIF DHLKNNVFII
160 170 180 190 200
SDSPEELTSK VLSPFEEKPE KNVFVTEPES NFEREQFYKV VEKAKKYIVE
210 220 230 240 250
GDIFQVVLSQ AFTFKTTLDP FYIYRALRMI NPSPYMFYLK FGDTVVLGSS
260 270 280 290 300
PETMAKVEGD KATVKPIAGT RPRGRTVEED LKLERELLND EKEIAEHVML
310 320 330 340 350
VDLGRNDLGR VCKEGTVRVE KKMVIERYSH VMHIVSQVSG ELKDDKDAVD
360 370 380 390 400
VFEATFPAGT VSGAPKVRAM EIIEELEPTP RGPYAGAVGY FSFPDDKGRM
410 420 430 440 450
NMDSAITIRS FFFKGKQGWL QAGAGIVYDS VPEREYQETL NKLRALFRSL
460
EVAQKIQGGL F
Length:461
Mass (Da):52,240
Last modified:May 30, 2000 - v2
Checksum:iDEA00256445C568C
GO

Sequence cautioni

The sequence CAA52202 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti104A → R in CAA52202 (PubMed:7685830).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74075 Genomic DNA. Translation: CAA52202.1. Different initiation.
AE000512 Genomic DNA. Translation: AAD35235.1.
PIRiD72414.
RefSeqiNP_227957.1. NC_000853.1.
WP_010865059.1. NC_000853.1.

Genome annotation databases

EnsemblBacteriaiAAD35235; AAD35235; TM_0142.
GeneIDi896972.
KEGGitma:TM0142.
tmi:THEMA_04095.
PATRICi23935126. VBITheMar51294_0141.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74075 Genomic DNA. Translation: CAA52202.1. Different initiation.
AE000512 Genomic DNA. Translation: AAD35235.1.
PIRiD72414.
RefSeqiNP_227957.1. NC_000853.1.
WP_010865059.1. NC_000853.1.

3D structure databases

ProteinModelPortaliQ08653.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0142.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35235; AAD35235; TM_0142.
GeneIDi896972.
KEGGitma:TM0142.
tmi:THEMA_04095.
PATRICi23935126. VBITheMar51294_0141.

Phylogenomic databases

eggNOGiENOG4105CRQ. Bacteria.
COG0147. LUCA.
InParanoidiQ08653.
KOiK01657.
OMAiTIRTLEM.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
BioCyciMetaCyc:MONOMER-281.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR015890. Chorismate_C.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRPE_THEMA
AccessioniPrimary (citable) accession number: Q08653
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: May 30, 2000
Last modified: November 2, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.