Q08653 (TRPE_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anthranilate synthase component 1
EC=4.1.3.27

Alternative name(s):
Anthranilate synthase component I

Gene names

Name:	trpE
Ordered Locus Names:	TM_0142

Organism

Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]

Taxonomic identifier

243274 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Thermotogae › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga › Thermotoga maritima

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Tetramer of two components I and two components II By similarity.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Belongs to the anthranilate synthase component I family.
Sequence caution	The sequence CAA52202.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Anthranilate synthase component 1		PRO_0000154116
Experimental info
	Sequence conflict	104	1	A → R in CAA52202. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q08653 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: DEA00256445C568C	FASTA	461	52,240
	10 20 30 40 50 60 MGDSMHVLKL VSDLETPVST FMKVSRGEEF AFLLESVELG SAFGRHSFIG IGKKDVLVFE 70 80 90 100 110 120 KGILRTSNQQ LDYTSSPLKA IKDWLEVYRY SVKHDELPSF RGGAVGFVSY DYISYIEKVK 130 140 150 160 170 180 VKASVFPTFY FVVPEHLIIF DHLKNNVFII SDSPEELTSK VLSPFEEKPE KNVFVTEPES 190 200 210 220 230 240 NFEREQFYKV VEKAKKYIVE GDIFQVVLSQ AFTFKTTLDP FYIYRALRMI NPSPYMFYLK 250 260 270 280 290 300 FGDTVVLGSS PETMAKVEGD KATVKPIAGT RPRGRTVEED LKLERELLND EKEIAEHVML 310 320 330 340 350 360 VDLGRNDLGR VCKEGTVRVE KKMVIERYSH VMHIVSQVSG ELKDDKDAVD VFEATFPAGT 370 380 390 400 410 420 VSGAPKVRAM EIIEELEPTP RGPYAGAVGY FSFPDDKGRM NMDSAITIRS FFFKGKQGWL 430 440 450 460 QAGAGIVYDS VPEREYQETL NKLRALFRSL EVAQKIQGGL F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Studies of the hyperthermophile Thermotoga maritima by random sequencing of cDNA and genomic libraries. Identification and sequencing of the trpEG (D) operon." Kim C.W., Markiewicz P.G., Lee J.J., Schierle C.F., Miller J.H. J. Mol. Biol. 231:960-981(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X74075 Genomic DNA. Translation: CAA52202.1. Different initiation. AE000512 Genomic DNA. Translation: AAD35235.1.
PIR	D72414.
RefSeq	NP_227957.1. NC_000853.1.
3D structure databases
ProteinModelPortal	Q08653.
ModBase	Search...
Protein-protein interaction databases
STRING	243274.TM0142.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD35235; AAD35235; TM_0142.
GeneID	896972.
KEGG	tma:TM0142.
PATRIC	23935126. VBITheMar51294_0141.
Phylogenomic databases
eggNOG	COG0147.
KO	K01657.
OMA	IERYEHV.
ProtClustDB	CLSK794948.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-281.
UniPathway	UPA00035; UER00040.
Family and domain databases
Gene3D	3.60.120.10. 1 hit.
InterPro	IPR005801. ADC_synthase. IPR019999. Anth_synth_I. IPR006805. Anth_synth_I_N. IPR015890. Chorismate-bd_C. [Graphical view]
Pfam	PF04715. Anth_synt_I_N. 1 hit. PF00425. Chorismate_bind. 1 hit. [Graphical view]
PRINTS	PR00095. ANTSNTHASEI.
SUPFAM	SSF56322. TRPE_1_chor_bd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRPE_THEMA

Accession

Primary (citable) accession number: Q08653

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	May 30, 2000
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program