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Protein

Diacylglycerol O-acyltransferase 1

Gene

DGA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis. May be involved in lipid particle synthesis from the endoplasmic reticulum and ergosterol biosynthesis. Also has monoacylglycerol acyltransferase (MGAT) activity, catalyzing the acyl-CoA-dependent esterification of monoacylglycerol to diacylglycerol.6 Publications

Catalytic activityi

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.1 Publication
Acyl-CoA + 2-acylglycerol = CoA + diacylglycerol.1 Publication

Pathwayi: triacylglycerol biosynthesis

This protein is involved in the pathway triacylglycerol biosynthesis, which is part of Glycerolipid metabolism.
View all proteins of this organism that are known to be involved in the pathway triacylglycerol biosynthesis and in Glycerolipid metabolism.

GO - Molecular functioni

GO - Biological processi

  • ceramide metabolic process Source: SGD
  • glycerol metabolic process Source: UniProtKB-KW
  • lipid storage Source: SGD
  • triglyceride biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Glycerol metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:YOR245C-MONOMER.
YEAST:YOR245C-MONOMER.
BRENDAi2.3.1.20. 984.
ReactomeiR-SCE-1482883. Acyl chain remodeling of DAG and TAG.
R-SCE-2142753. Arachidonic acid metabolism.
R-SCE-535734. Fatty acid, triacylglycerol, and ketone body metabolism.
R-SCE-75109. Triglyceride Biosynthesis.
R-SCE-8848584. Wax biosynthesis.
UniPathwayiUPA00282.

Chemistry databases

SwissLipidsiSLP:000000051.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol O-acyltransferase 1 (EC:2.3.1.20)
Short name:
DGAT
Alternative name(s):
Acyl-CoA:monoacylglycerol acyltransferase (EC:2.3.1.22)
Short name:
MGAT
Gene namesi
Name:DGA1
Ordered Locus Names:YOR245C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR245C.
SGDiS000005771. DGA1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 71CytoplasmicSequence analysisAdd BLAST71
Transmembranei72 – 92HelicalSequence analysisAdd BLAST21
Topological domaini93 – 186LumenalSequence analysisAdd BLAST94
Transmembranei187 – 207HelicalSequence analysisAdd BLAST21
Topological domaini208 – 215CytoplasmicSequence analysis8
Transmembranei216 – 236HelicalSequence analysisAdd BLAST21
Topological domaini237 – 289LumenalSequence analysisAdd BLAST53
Transmembranei290 – 310HelicalSequence analysisAdd BLAST21
Topological domaini311 – 418CytoplasmicSequence analysisAdd BLAST108

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: SGD
  • lipid particle Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi71F → A: Retains more than 40% of the wild-type enzyme activity. 1 Publication1
Mutagenesisi73L → A: Retains more than 40% of the wild-type enzyme activity. 1 Publication1
Mutagenesisi129 – 131YFP → AA: Almost complete loss of enzyme activity. 1 Publication3
Mutagenesisi193 – 196HPHG → EPHS: Complete loss of enzyme activity. 1 Publication4
Mutagenesisi193H → A: Almost complete loss of enzyme activity. 1 Publication1
Mutagenesisi195H → A: Complete loss of enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002330011 – 418Diacylglycerol O-acyltransferase 1Add BLAST418

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08650.
PRIDEiQ08650.

PTM databases

iPTMnetiQ08650.

Interactioni

Protein-protein interaction databases

BioGridi34636. 41 interactors.
DIPiDIP-2923N.
IntActiQ08650. 2 interactors.
MINTiMINT-546626.

Structurei

3D structure databases

ProteinModelPortaliQ08650.
SMRiQ08650.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00750000117391.
HOGENOMiHOG000179738.
InParanoidiQ08650.
KOiK14457.
OMAiSWTIWRY.
OrthoDBiEOG092C1H3C.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PfamiPF03982. DAGAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08650-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGTFNDIRR RKKEEGSPTA GITERHENKS LSSIDKREQT LKPQLESCCP
60 70 80 90 100
LATPFERRLQ TLAVAWHTSS FVLFSIFTLF AISTPALWVL AIPYMIYFFF
110 120 130 140 150
DRSPATGEVV NRYSLRFRSL PIWKWYCDYF PISLIKTVNL KPTFTLSKNK
160 170 180 190 200
RVNEKNYKIR LWPTKYSINL KSNSTIDYRN QECTGPTYLF GYHPHGIGAL
210 220 230 240 250
GAFGAFATEG CNYSKIFPGI PISLMTLVTQ FHIPLYRDYL LALGISSVSR
260 270 280 290 300
KNALRTLSKN QSICIVVGGA RESLLSSTNG TQLILNKRKG FIKLAIQTGN
310 320 330 340 350
INLVPVFAFG EVDCYNVLST KKDSVLGKMQ LWFKENFGFT IPIFYARGLF
360 370 380 390 400
NYDFGLLPFR APINVVVGRP IYVEKKITNP PDDVVNHFHD LYIAELKRLY
410
YENREKYGVP DAELKIVG
Length:418
Mass (Da):47,711
Last modified:November 1, 1996 - v1
Checksum:iE6ECAA95219583BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75153 Genomic DNA. Translation: CAA99466.1.
BK006948 Genomic DNA. Translation: DAA11013.1.
PIRiS67138.
RefSeqiNP_014888.1. NM_001183664.1.

Genome annotation databases

EnsemblFungiiYOR245C; YOR245C; YOR245C.
GeneIDi854419.
KEGGisce:YOR245C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75153 Genomic DNA. Translation: CAA99466.1.
BK006948 Genomic DNA. Translation: DAA11013.1.
PIRiS67138.
RefSeqiNP_014888.1. NM_001183664.1.

3D structure databases

ProteinModelPortaliQ08650.
SMRiQ08650.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34636. 41 interactors.
DIPiDIP-2923N.
IntActiQ08650. 2 interactors.
MINTiMINT-546626.

Chemistry databases

SwissLipidsiSLP:000000051.

PTM databases

iPTMnetiQ08650.

Proteomic databases

MaxQBiQ08650.
PRIDEiQ08650.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR245C; YOR245C; YOR245C.
GeneIDi854419.
KEGGisce:YOR245C.

Organism-specific databases

EuPathDBiFungiDB:YOR245C.
SGDiS000005771. DGA1.

Phylogenomic databases

GeneTreeiENSGT00750000117391.
HOGENOMiHOG000179738.
InParanoidiQ08650.
KOiK14457.
OMAiSWTIWRY.
OrthoDBiEOG092C1H3C.

Enzyme and pathway databases

UniPathwayiUPA00282.
BioCyciMetaCyc:YOR245C-MONOMER.
YEAST:YOR245C-MONOMER.
BRENDAi2.3.1.20. 984.
ReactomeiR-SCE-1482883. Acyl chain remodeling of DAG and TAG.
R-SCE-2142753. Arachidonic acid metabolism.
R-SCE-535734. Fatty acid, triacylglycerol, and ketone body metabolism.
R-SCE-75109. Triglyceride Biosynthesis.
R-SCE-8848584. Wax biosynthesis.

Miscellaneous databases

PROiQ08650.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PfamiPF03982. DAGAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDGAT2_YEAST
AccessioniPrimary (citable) accession number: Q08650
Secondary accession number(s): D6W2U7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 907 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.