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Protein

Diacylglycerol O-acyltransferase 1

Gene

DGA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis. May be involved in lipid particle synthesis from the endoplasmic reticulum and ergosterol biosynthesis. Also has monoacylglycerol acyltransferase (MGAT) activity, catalyzing the acyl-CoA-dependent esterification of monoacylglycerol to diacylglycerol.6 Publications

Catalytic activityi

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.1 Publication
Acyl-CoA + 2-acylglycerol = CoA + diacylglycerol.1 Publication

Pathwayi: triacylglycerol biosynthesis

This protein is involved in the pathway triacylglycerol biosynthesis, which is part of Glycerolipid metabolism.
View all proteins of this organism that are known to be involved in the pathway triacylglycerol biosynthesis and in Glycerolipid metabolism.

GO - Molecular functioni

GO - Biological processi

  • ceramide metabolic process Source: SGD
  • glycerol metabolic process Source: UniProtKB-KW
  • lipid storage Source: SGD
  • triglyceride biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Glycerol metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:YOR245C-MONOMER.
YEAST:YOR245C-MONOMER.
BRENDAi2.3.1.20. 984.
ReactomeiR-SCE-1482883. Acyl chain remodeling of DAG and TAG.
R-SCE-2142753. Arachidonic acid metabolism.
R-SCE-535734. Fatty acid, triacylglycerol, and ketone body metabolism.
R-SCE-75109. Triglyceride Biosynthesis.
R-SCE-8848584. Wax biosynthesis.
UniPathwayiUPA00282.

Chemistry

SwissLipidsiSLP:000000051.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol O-acyltransferase 1 (EC:2.3.1.20)
Short name:
DGAT
Alternative name(s):
Acyl-CoA:monoacylglycerol acyltransferase (EC:2.3.1.22)
Short name:
MGAT
Gene namesi
Name:DGA1
Ordered Locus Names:YOR245C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR245C.
SGDiS000005771. DGA1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7171CytoplasmicSequence analysisAdd
BLAST
Transmembranei72 – 9221HelicalSequence analysisAdd
BLAST
Topological domaini93 – 18694LumenalSequence analysisAdd
BLAST
Transmembranei187 – 20721HelicalSequence analysisAdd
BLAST
Topological domaini208 – 2158CytoplasmicSequence analysis
Transmembranei216 – 23621HelicalSequence analysisAdd
BLAST
Topological domaini237 – 28953LumenalSequence analysisAdd
BLAST
Transmembranei290 – 31021HelicalSequence analysisAdd
BLAST
Topological domaini311 – 418108CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: SGD
  • lipid particle Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi71 – 711F → A: Retains more than 40% of the wild-type enzyme activity. 1 Publication
Mutagenesisi73 – 731L → A: Retains more than 40% of the wild-type enzyme activity. 1 Publication
Mutagenesisi129 – 1313YFP → AA: Almost complete loss of enzyme activity. 1 Publication
Mutagenesisi193 – 1964HPHG → EPHS: Complete loss of enzyme activity. 1 Publication
Mutagenesisi193 – 1931H → A: Almost complete loss of enzyme activity. 1 Publication
Mutagenesisi195 – 1951H → A: Complete loss of enzyme activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 418418Diacylglycerol O-acyltransferase 1PRO_0000233001Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei17 – 171PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08650.

PTM databases

iPTMnetiQ08650.

Interactioni

Protein-protein interaction databases

BioGridi34636. 41 interactions.
DIPiDIP-2923N.
IntActiQ08650. 2 interactions.
MINTiMINT-546626.

Structurei

3D structure databases

ProteinModelPortaliQ08650.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00750000117391.
HOGENOMiHOG000179738.
InParanoidiQ08650.
KOiK14457.
OMAiWFLYVPY.
OrthoDBiEOG7K3TW0.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PfamiPF03982. DAGAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08650-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGTFNDIRR RKKEEGSPTA GITERHENKS LSSIDKREQT LKPQLESCCP
60 70 80 90 100
LATPFERRLQ TLAVAWHTSS FVLFSIFTLF AISTPALWVL AIPYMIYFFF
110 120 130 140 150
DRSPATGEVV NRYSLRFRSL PIWKWYCDYF PISLIKTVNL KPTFTLSKNK
160 170 180 190 200
RVNEKNYKIR LWPTKYSINL KSNSTIDYRN QECTGPTYLF GYHPHGIGAL
210 220 230 240 250
GAFGAFATEG CNYSKIFPGI PISLMTLVTQ FHIPLYRDYL LALGISSVSR
260 270 280 290 300
KNALRTLSKN QSICIVVGGA RESLLSSTNG TQLILNKRKG FIKLAIQTGN
310 320 330 340 350
INLVPVFAFG EVDCYNVLST KKDSVLGKMQ LWFKENFGFT IPIFYARGLF
360 370 380 390 400
NYDFGLLPFR APINVVVGRP IYVEKKITNP PDDVVNHFHD LYIAELKRLY
410
YENREKYGVP DAELKIVG
Length:418
Mass (Da):47,711
Last modified:November 1, 1996 - v1
Checksum:iE6ECAA95219583BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75153 Genomic DNA. Translation: CAA99466.1.
BK006948 Genomic DNA. Translation: DAA11013.1.
PIRiS67138.
RefSeqiNP_014888.1. NM_001183664.1.

Genome annotation databases

EnsemblFungiiYOR245C; YOR245C; YOR245C.
GeneIDi854419.
KEGGisce:YOR245C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75153 Genomic DNA. Translation: CAA99466.1.
BK006948 Genomic DNA. Translation: DAA11013.1.
PIRiS67138.
RefSeqiNP_014888.1. NM_001183664.1.

3D structure databases

ProteinModelPortaliQ08650.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34636. 41 interactions.
DIPiDIP-2923N.
IntActiQ08650. 2 interactions.
MINTiMINT-546626.

Chemistry

SwissLipidsiSLP:000000051.

PTM databases

iPTMnetiQ08650.

Proteomic databases

MaxQBiQ08650.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR245C; YOR245C; YOR245C.
GeneIDi854419.
KEGGisce:YOR245C.

Organism-specific databases

EuPathDBiFungiDB:YOR245C.
SGDiS000005771. DGA1.

Phylogenomic databases

GeneTreeiENSGT00750000117391.
HOGENOMiHOG000179738.
InParanoidiQ08650.
KOiK14457.
OMAiWFLYVPY.
OrthoDBiEOG7K3TW0.

Enzyme and pathway databases

UniPathwayiUPA00282.
BioCyciMetaCyc:YOR245C-MONOMER.
YEAST:YOR245C-MONOMER.
BRENDAi2.3.1.20. 984.
ReactomeiR-SCE-1482883. Acyl chain remodeling of DAG and TAG.
R-SCE-2142753. Arachidonic acid metabolism.
R-SCE-535734. Fatty acid, triacylglycerol, and ketone body metabolism.
R-SCE-75109. Triglyceride Biosynthesis.
R-SCE-8848584. Wax biosynthesis.

Miscellaneous databases

PROiQ08650.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PfamiPF03982. DAGAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Synthesis of triacylglycerols by the acyl-coenzyme A:diacyl-glycerol acyltransferase Dga1p in lipid particles of the yeast Saccharomyces cerevisiae."
    Sorger D., Daum G.
    J. Bacteriol. 184:519-524(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. Cited for: FUNCTION.
  5. "The DGA1 gene determines a second triglyceride synthetic pathway in yeast."
    Oelkers P., Cromley D., Padamsee M., Billheimer J.T., Sturley S.L.
    J. Biol. Chem. 277:8877-8881(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Lipid dynamics in yeast under haem-induced unsaturated fatty acid and/or sterol depletion."
    Ferreira T., Regnacq M., Alimardani P., Moreau-Vauzelle C., Berges T.
    Biochem. J. 378:899-908(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "A yeast strain lacking lipid particles bears a defect in ergosterol formation."
    Sorger D., Athenstaedt K., Hrastnik C., Daum G.
    J. Biol. Chem. 279:31190-31196(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "Identification of Yju3p as functional orthologue of mammalian monoglyceride lipase in the yeast Saccharomycescerevisiae."
    Heier C., Taschler U., Rengachari S., Oberer M., Wolinski H., Natter K., Kohlwein S.D., Leber R., Zimmermann R.
    Biochim. Biophys. Acta 1801:1063-1071(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY.
  12. "Functional and topological analysis of yeast acyl-CoA:diacylglycerol acyltransferase 2, an endoplasmic reticulum enzyme essential for triacylglycerol biosynthesis."
    Liu Q., Siloto R.M., Snyder C.L., Weselake R.J.
    J. Biol. Chem. 286:13115-13126(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TOPOLOGY, MUTAGENESIS OF PHE-71; LEU-73; 129-TYR--PRO-131; HIS-193; 193-HIS--GLY-196 AND HIS-195.

Entry informationi

Entry nameiDGAT2_YEAST
AccessioniPrimary (citable) accession number: Q08650
Secondary accession number(s): D6W2U7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 907 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.