Q08650 (DGA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol O-acyltransferase 1
EC=2.3.1.20

Gene names

Name:	DGA1
Ordered Locus Names:	YOR245C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis. May be involved in lipid particle synthesis from the endoplasmic reticulum and ergosterol biosynthesis. Ref.3 Ref.4 Ref.5 Ref.8 Ref.9
Catalytic activity	Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
Pathway	Glycerolipid metabolism; triacylglycerol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein Potential. Lipid droplet Ref.3 Ref.6.
Miscellaneous	Present with 907 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the diacylglycerol acyltransferase family.

Ontologies

Keywords
Biological process	Glycerol metabolism Lipid metabolism Lipid synthesis
Cellular component	Endoplasmic reticulum Lipid droplet Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW lipid storage Inferred from mutant phenotype Ref.3. Source: SGD triglyceride biosynthetic process Inferred from direct assay Ref.3. Source: SGD
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from direct assay. Source: SGD lipid particle Inferred from direct assay Ref.3. Source: SGD
Molecular function	diacylglycerol O-acyltransferase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PDC1	P06169	1	EBI-33586,EBI-5687

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	Diacylglycerol O-acyltransferase 1		PRO_0000233001
Regions
	Transmembrane	72 – 92	21	Helical; Potential
Amino acid modifications
	Modified residue	17	1	Phosphoserine Ref.10 Ref.11

Sequences

	Sequence		Length	Mass (Da)
	Q08650 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E6ECAA95219583BD	FASTA	418	47,711
	10 20 30 40 50 60 MSGTFNDIRR RKKEEGSPTA GITERHENKS LSSIDKREQT LKPQLESCCP LATPFERRLQ 70 80 90 100 110 120 TLAVAWHTSS FVLFSIFTLF AISTPALWVL AIPYMIYFFF DRSPATGEVV NRYSLRFRSL 130 140 150 160 170 180 PIWKWYCDYF PISLIKTVNL KPTFTLSKNK RVNEKNYKIR LWPTKYSINL KSNSTIDYRN 190 200 210 220 230 240 QECTGPTYLF GYHPHGIGAL GAFGAFATEG CNYSKIFPGI PISLMTLVTQ FHIPLYRDYL 250 260 270 280 290 300 LALGISSVSR KNALRTLSKN QSICIVVGGA RESLLSSTNG TQLILNKRKG FIKLAIQTGN 310 320 330 340 350 360 INLVPVFAFG EVDCYNVLST KKDSVLGKMQ LWFKENFGFT IPIFYARGLF NYDFGLLPFR 370 380 390 400 410 APINVVVGRP IYVEKKITNP PDDVVNHFHD LYIAELKRLY YENREKYGVP DAELKIVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Synthesis of triacylglycerols by the acyl-coenzyme A:diacyl-glycerol acyltransferase Dga1p in lipid particles of the yeast Saccharomyces cerevisiae." Sorger D., Daum G. J. Bacteriol. 184:519-524(2002) [PubMed: 11751830] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]	"Storage lipid synthesis is non-essential in yeast." Sandager L., Gustavsson M.H., Staahl U., Dahlqvist A., Wiberg E., Banas A., Lenman M., Ronne H., Stymne S. J. Biol. Chem. 277:6478-6482(2002) [PubMed: 11741946] [Abstract] Cited for: FUNCTION.
[5]	"The DGA1 gene determines a second triglyceride synthetic pathway in yeast." Oelkers P., Cromley D., Padamsee M., Billheimer J.T., Sturley S.L. J. Biol. Chem. 277:8877-8881(2002) [PubMed: 11751875] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Lipid dynamics in yeast under haem-induced unsaturated fatty acid and/or sterol depletion." Ferreira T., Regnacq M., Alimardani P., Moreau-Vauzelle C., Berges T. Biochem. J. 378:899-908(2004) [PubMed: 14640980] [Abstract] Cited for: FUNCTION.
[9]	"A yeast strain lacking lipid particles bears a defect in ergosterol formation." Sorger D., Athenstaedt K., Hrastnik C., Daum G. J. Biol. Chem. 279:31190-31196(2004) [PubMed: 15155725] [Abstract] Cited for: FUNCTION.
[10]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, MASS SPECTROMETRY. Strain: ADR376.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z75153 Genomic DNA. Translation: CAA99466.1. BK006948 Genomic DNA. Translation: DAA11013.1.
PIR	S67138.
RefSeq	NP_014888.1. NM_001183664.1.
3D structure databases
ProteinModelPortal	Q08650.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2923N.
IntAct	Q08650. 2 interactions.
MINT	MINT-546626.
STRING	Q08650.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR245C; YOR245C; YOR245C.
GeneID	854419.
KEGG	sce:YOR245C.
NMPDR	fig|4932.3.peg.6000.
Organism-specific databases
CYGD	YOR245c.
SGD	S000005771. DGA1.
Phylogenomic databases
eggNOG	fuNOG04748.
GeneTree	EFGT00050000001265.
HOGENOM	HBG561030.
OMA	VAWHTSS.
OrthoDB	EOG4M3DJ3.
Gene expression databases
ArrayExpress	Q08650.
Genevestigator	Q08650.
GermOnline	YOR245C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007130. DAGAT. [Graphical view]
KO	K14457.
PANTHER	PTHR12317. DAGAT. 1 hit.
Pfam	PF03982. DAGAT. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	976625.

Entry information

Entry name

DGA1_YEAST

Accession

Primary (citable) accession number: Q08650
Secondary accession number(s): D6W2U7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program