Q08648 (SG11B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sperm-associated antigen 11B

Alternative name(s):
Human epididymis-specific protein 2
Short name=He2
Protein EP2
Sperm antigen HE2

Gene names

Name:	SPAG11B
Synonyms:	EP2, HE2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	103 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Secreted.
Tissue specificity	Specifically expressed in caput and proximal corpus of epididymis. Present in the epididymal epithelium and on the sperm surface, with a subacrosomal equatorial distribution on the sperm head.
Polymorphism	The microheterogeneity probably represents an allelic variant.
Sequence similarities	Belongs to the SPAG11 family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	spermatogenesis Traceable author statement Ref.1. Source: ProtInc
Cellular_component	extracellular region Non-traceable author statement Ref.2. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Note: Exon 6 is read in two different reading frames, one resulting in the N-terminal amino acid sequences of EP2A, EP2B and EP2F and the other in the N-terminal sequences of EP2D, EP2E and EP2G.

Isoform EP2A (identifier: Q08648-1)

Also known as: HE2 alpha1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform EP2B (identifier: Q08648-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-71: MRQRLLPSVT...LLPPRTPPYQ → MKVFFLFAVLFCLVQTNS

Isoform EP2C (identifier: Q08648-3)

Also known as: HE2C;

The sequence of this isoform differs from the canonical sequence as follows:
     72-103: VHISHREARGPSFRICVDFLGPRWARGCSTGN → EPASDLKVVDCRRSEGFCQEYCNYMETQVGYCSKKKDACCLH

Isoform EP2D (identifier: Q08648-4)

Also known as: HE2 beta1;

The sequence of this isoform differs from the canonical sequence as follows:
     72-103: VHISHREARGPSFRICVDFLGPRWARGCSTGN → GDVPPGIRNT...KPEMDGRSGI

Isoform EP2E (identifier: Q08648-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-80: MRQRLLPSVT...QVHISHREAR → MKVFFLFAVL...KPEMDGRSGI
     81-103: Missing.

Isoform EP2F (identifier: Q08648-6)

The sequence of this isoform is not available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 103	78	Sperm-associated antigen 11B		PRO_0000033185
Amino acid modifications
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 80	80	MRQRL…HREAR → MKVFFLFAVLFCLVQTNSGD VPPGIRNTICHMQQGICRLF FCHSGEKKRDICSDPWNRCC VSNTDEEGKEKPEMDGRSGI in isoform EP2E.		VSP_044926
	Alternative sequence	1 – 71	71	MRQRL…TPPYQ → MKVFFLFAVLFCLVQTNS in isoform EP2B.		VSP_006208
	Alternative sequence	72 – 103	32	VHISH…CSTGN → EPASDLKVVDCRRSEGFCQE YCNYMETQVGYCSKKKDACC LH in isoform EP2C.		VSP_006209
	Alternative sequence	72 – 103	32	VHISH…CSTGN → GDVPPGIRNTICRMQQGICR LFFCHSGEKKRDICSDPWNR CCVSNTDEEGKEKPEMDGRS GI in isoform EP2D.		VSP_006210
	Alternative sequence	81 – 103	23	Missing in isoform EP2E.		VSP_044927
	Natural variant	77	1	R → Q. Ref.3 Corresponds to variant rs2853658 [ dbSNP | Ensembl ].		VAR_005269
	Natural variant	89	1	D → G. Corresponds to variant rs2738035 [ dbSNP | Ensembl ].		VAR_053683
Experimental info
Isoform EP2C:
	Sequence conflict	18	1	L → C in CAG25732. Ref.4
Isoform EP2E:
	Sequence conflict	31	1	H → R in AAG21882. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform EP2A (HE2 alpha1) [UniParc]. Last modified April 18, 2006. Version 4. Checksum: ABDD1CE4BB54ABD3	FASTA	103	11,431
	10 20 30 40 50 60 MRQRLLPSVT SLLLVALLFP GSSQARHVNH SATEALGELR ERAPGQGTNG FQLLRHAVKR 70 80 90 100 DLLPPRTPPY QVHISHREAR GPSFRICVDF LGPRWARGCS TGN
	Isoform EP2B [UniParc]. Checksum: A91640B5802DCDD1	FASTA	50	5,673
	10 20 30 40 50 MKVFFLFAVL FCLVQTNSVH ISHREARGPS FRICVDFLGP RWARGCSTGN
	Isoform EP2C (HE2C) [UniParc]. Checksum: F962A417664352A3	FASTA	113	12,687
	10 20 30 40 50 60 MRQRLLPSVT SLLLVALLFP GSSQARHVNH SATEALGELR ERAPGQGTNG FQLLRHAVKR 70 80 90 100 110 DLLPPRTPPY QEPASDLKVV DCRRSEGFCQ EYCNYMETQV GYCSKKKDAC CLH
	Isoform EP2D (HE2 beta1) [UniParc]. Checksum: B8D758090CE5651A	FASTA	133	14,850
	10 20 30 40 50 60 MRQRLLPSVT SLLLVALLFP GSSQARHVNH SATEALGELR ERAPGQGTNG FQLLRHAVKR 70 80 90 100 110 120 DLLPPRTPPY QGDVPPGIRN TICRMQQGIC RLFFCHSGEK KRDICSDPWN RCCVSNTDEE 130 GKEKPEMDGR SGI
	Isoform EP2E [UniParc]. Checksum: CF2C3F5E0C084019	FASTA	80	9,072
	10 20 30 40 50 60 MKVFFLFAVL FCLVQTNSGD VPPGIRNTIC HMQQGICRLF FCHSGEKKRD ICSDPWNRCC 70 80 VSNTDEEGKE KPEMDGRSGI
	Isoform EP2F (Sequence not available).		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a novel human sperm antigen (HE2) specifically expressed in the proximal epididymis." Osterhoff C., Kirchhoff C., Krull N., Ivell R. Biol. Reprod. 50:516-525(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM EP2A).
[2]	"HE2 beta and HE2 gamma, new members of an epididymis-specific family of androgen-regulated proteins in the human." Hamil K.G., Sivashanmugam P., Richardson R.T., Grossman G., Ruben S.M., Mohler J.L., Petrusz P., O'Rand M.G., French F.S., Hall S.H. Endocrinology 141:1245-1253(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS EP2A AND EP2D).
[3]	"Organization of the human gene encoding the epididymis-specific EP2 protein variants and its relationship to defensin genes." Frohlich O., Po C., Young L.G. Biol. Reprod. 64:1072-1079(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS EP2A; EP2B; EP2C; EP2D AND EP2E), VARIANT GLN-77.
[4]	"SPAG11/Isoform HE2C, an atypical anionic beta-defensin-like peptide of the proximal human epididymis." von Horsten H.H., Derr P., Schaefer B., Kirchhoff C. Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM EP2C).
[5]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X67697 mRNA. Translation: CAA47927.1. AF168616 mRNA. Translation: AAF37186.1. AF168617 mRNA. Translation: AAF37187.1. AY005129 Genomic DNA. Translation: AAG21878.1. AY005129 Genomic DNA. Translation: AAG21879.1. AY005129 Genomic DNA. Translation: AAG21880.1. AY005129 Genomic DNA. Translation: AAG21881.1. AY005129 Genomic DNA. Translation: AAG21882.1. AY005129 Genomic DNA. Translation: AAG21883.1. AJ628017 mRNA. Translation: CAF31328.1. AJ635328 mRNA. Translation: CAG25732.1. AC130360 Genomic DNA. No translation available.
IPI	IPI00220126. IPI00220127. IPI00792134. IPI00793278. IPI00794932.
PIR	I37454.
RefSeq	NP_057596.1. NM_016512.3. NP_478107.2. NM_058200.2. NP_478108.2. NM_058201.2. NP_478109.1. NM_058202.2. NP_478110.1. NM_058203.2. NP_478113.2. NM_058206.3. NP_478114.2. NM_058207.2.
UniGene	Hs.2717.
3D structure databases
ProteinModelPortal	Q08648.
ModBase	Search...
Polymorphism databases
DMDM	93141314.
Proteomic databases
PRIDE	Q08648.
Protocols and materials databases
DNASU	10407.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000297498; ENSP00000297498; ENSG00000164871. ENST00000317900; ENSP00000322591; ENSG00000164871. ENST00000458665; ENSP00000398550; ENSG00000164871. ENST00000528168; ENSP00000431230; ENSG00000164871.
GeneID	10407.
KEGG	hsa:10407.
UCSC	uc003wri.3. human. uc003wrk.3. human. uc003wrm.3. human.
Organism-specific databases
CTD	10407.
GeneCards	GC08M007294.
HGNC	HGNC:14534. SPAG11B.
MIM	606560. gene.
neXtProt	NX_Q08648.
PharmGKB	PA37894.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG121990.
HOVERGEN	HBG073275.
OMA	NCKKSEG.
Gene expression databases
ArrayExpress	Q08648.
Bgee	Q08648.
Genevestigator	Q08648.
GermOnline	ENSG00000164871. Homo sapiens.
Family and domain databases
InterPro	IPR007988. Sperm_Ag_HE2. [Graphical view]
Pfam	PF05324. Sperm_Ag_HE2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SPAG11B. human.
GenomeRNAi	10407.
NextBio	39435.
SOURCE	Search...

Entry information

Entry name

SG11B_HUMAN

Accession

Primary (citable) accession number: Q08648
Secondary accession number(s): E9PFH0 , Q546A0, Q6ZYB2, Q9H4P8, Q9H4P9, Q9H4Q0, Q9H4Q1, Q9H4Q2, Q9NRT3, Q9NRV4, Q9NRV5, Q9NRV6, Q9NRV7, Q9NRV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	April 18, 2006
Last modified:	April 3, 2013
This is version 95 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.