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Protein

Multisubstrate pseudouridine synthase 7

Gene

PUS7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes pseudouridylation at position 35 in U2 snRNA stem-loop II region which induces particular conformation of the mRNA-U2 snRNA duplex and places the nucleophile in an accessible position for the first step of splicing. Catalyzes also pseudouridylation at position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr). Pseudouridine residues in tRNAs may stabilize the local RNA conformation, favor interactions with protein partners and play an important role in the stabilization of the codon-anticodon interaction with mRNA.7 Publications

Miscellaneous

Present with 4150 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

rRNA uridine = rRNA pseudouridine.1 Publication
snRNA uridine = snRNA pseudouridine.1 Publication
tRNA uridine(13) = tRNA pseudouridine(13).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei256Nucleophile1

GO - Molecular functioni

  • pseudouridine synthase activity Source: SGD
  • RNA binding Source: InterPro

GO - Biological processi

  • enzyme-directed rRNA pseudouridine synthesis Source: SGD
  • mRNA processing Source: UniProtKB-KW
  • mRNA pseudouridine synthesis Source: SGD
  • RNA splicing Source: UniProtKB-KW
  • snRNA pseudouridine synthesis Source: SGD
  • tRNA modification Source: Reactome
  • tRNA pseudouridine synthesis Source: SGD

Keywordsi

Molecular functionIsomerase
Biological processmRNA processing, mRNA splicing, Ribosome biogenesis, rRNA processing, tRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-33737-MONOMER
BRENDAi5.4.99.27 984

Names & Taxonomyi

Protein namesi
Recommended name:
Multisubstrate pseudouridine synthase 7 (EC:5.4.99.27)
Alternative name(s):
RNA pseudouridylate synthase 7
RNA-uridine isomerase 7
tRNA pseudouridine(13) synthase
Gene namesi
Name:PUS7
Ordered Locus Names:YOR243C
ORF Names:O5254
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR243C
SGDiS000005769 PUS7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi256D → A: Abolishes enzymatic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001525612 – 676Multisubstrate pseudouridine synthase 7Add BLAST675

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ08647
PaxDbiQ08647
PRIDEiQ08647
TopDownProteomicsiQ08647

PTM databases

iPTMnetiQ08647

Interactioni

Protein-protein interaction databases

BioGridi34634, 146 interactors
DIPiDIP-6762N
IntActiQ08647, 11 interactors
MINTiQ08647
STRINGi4932.YOR243C

Structurei

3D structure databases

ProteinModelPortaliQ08647
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini338 – 582TRUDPROSITE-ProRule annotationAdd BLAST245

Sequence similaritiesi

Belongs to the pseudouridine synthase TruD family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063554
HOGENOMiHOG000203484
InParanoidiQ08647
KOiK06176
OMAiPGEHPDV
OrthoDBiEOG092C1FRM

Family and domain databases

InterProiView protein in InterPro
IPR020103 PsdUridine_synth_cat_dom_sf
IPR001656 PsdUridine_synth_TruD
IPR020119 PsdUridine_synth_TruD_CS
IPR011760 PsdUridine_synth_TruD_insert
PANTHERiPTHR13326 PTHR13326, 1 hit
PfamiView protein in Pfam
PF01142 TruD, 1 hit
PIRSFiPIRSF037016 Pseudouridin_synth_euk_prd, 1 hit
SUPFAMiSSF55120 SSF55120, 4 hits
TIGRFAMsiTIGR00094 tRNA_TruD_broad, 1 hit
PROSITEiView protein in PROSITE
PS50984 TRUD, 1 hit
PS01268 UPF0024, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08647-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSSEATVK RPLDAHVGPS ENAAKKLKIE QRTQADGIHE ADVGITLFLS
60 70 80 90 100
PELPGFRGQI KQRYTDFLVN EIDQEGKVIH LTDKGFKMPK KPQRSKEEVN
110 120 130 140 150
AEKESEAARR QEFNVDPELR NQLVEIFGEE DVLKIESVYR TANKMETAKN
160 170 180 190 200
FEDKSVRTKI HQLLREAFKN ELESVTTDTN TFKIARSNRN SRTNKQEKIN
210 220 230 240 250
QTRDANGVEN WGYGPSKDFI HFTLHKENKD TMEAVNVITK LLRVPSRVIR
260 270 280 290 300
YAGTKDRRAV TCQRVSISKI GLDRLNALNR TLKGMIIGNY NFSDASLNLG
310 320 330 340 350
DLKGNEFVVV IRDVTTGNSE VSLEEIVSNG CKSLSENGFI NYFGMQRFGT
360 370 380 390 400
FSISTHTIGR ELLLSNWKKA AELILSDQDN VLPKSKEARK IWAETKDAAL
410 420 430 440 450
ALKQMPRQCL AENALLYSLS NQRKEEDGTY SENAYYTAIM KIPRNLRTMY
460 470 480 490 500
VHAYQSYVWN SIASKRIELH GLKLVVGDLV IDTSEKSPLI SGIDDEDFDE
510 520 530 540 550
DVREAQFIRA KAVTQEDIDS VKYTMEDVVL PSPGFDVLYP SNEELKQLYV
560 570 580 590 600
DILKADNMDP FNMRRKVRDF SLAGSYRTVI QKPKSLEYRI IHYDDPSQQL
610 620 630 640 650
VNTDLDILNN TRAKESGQKY MKAKLDRYMP DKGGEKTAVV LKFQLGTSAY
660 670
ATMALRELMK LETSRRGDMC DVKENI
Length:676
Mass (Da):77,002
Last modified:November 1, 1996 - v1
Checksum:i4B63F137B781FCCB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75151 Genomic DNA Translation: CAA99464.1
BK006948 Genomic DNA Translation: DAA11011.1
PIRiS67136
RefSeqiNP_014886.1, NM_001183662.1

Genome annotation databases

EnsemblFungiiYOR243C; YOR243C; YOR243C
GeneIDi854417
KEGGisce:YOR243C

Similar proteinsi

Entry informationi

Entry nameiPUS7_YEAST
AccessioniPrimary (citable) accession number: Q08647
Secondary accession number(s): D6W2U5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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