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Protein

Multisubstrate pseudouridine synthase 7

Gene

PUS7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes pseudouridylation at position 35 in U2 snRNA stem-loop II region which induces particular conformation of the mRNA-U2 snRNA duplex and places the nucleophile in an accessible position for the first step of splicing. Catalyzes also pseudouridylation at position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr). Pseudouridine residues in tRNAs may stabilize the local RNA conformation, favor interactions with protein partners and play an important role in the stabilization of the codon-anticodon interaction with mRNA.7 Publications

Catalytic activityi

rRNA uridine = rRNA pseudouridine.1 Publication
snRNA uridine = snRNA pseudouridine.1 Publication
tRNA uridine(13) = tRNA pseudouridine(13).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei256 – 2561Nucleophile

GO - Molecular functioni

  • pseudouridine synthase activity Source: SGD
  • RNA binding Source: InterPro

GO - Biological processi

  • enzyme-directed rRNA pseudouridine synthesis Source: SGD
  • mRNA processing Source: UniProtKB-KW
  • mRNA pseudouridine synthesis Source: SGD
  • RNA splicing Source: UniProtKB-KW
  • snRNA pseudouridine synthesis Source: SGD
  • tRNA modification Source: Reactome
  • tRNA pseudouridine synthesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

mRNA processing, mRNA splicing, Ribosome biogenesis, rRNA processing, tRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-33737-MONOMER.
BRENDAi5.4.99.27. 984.
ReactomeiR-SCE-6782315. tRNA modification in the nucleus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Multisubstrate pseudouridine synthase 7 (EC:5.4.99.27)
Alternative name(s):
RNA pseudouridylate synthase 7
RNA-uridine isomerase 7
tRNA pseudouridine(13) synthase
Gene namesi
Name:PUS7
Ordered Locus Names:YOR243C
ORF Names:O5254
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR243C.
SGDiS000005769. PUS7.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleoplasm Source: Reactome
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi256 – 2561D → A: Abolishes enzymatic activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 676675Multisubstrate pseudouridine synthase 7PRO_0000152561Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ08647.
TopDownProteomicsiQ08647.

Interactioni

Protein-protein interaction databases

BioGridi34634. 53 interactions.
DIPiDIP-6762N.
IntActiQ08647. 11 interactions.
MINTiMINT-617058.

Structurei

3D structure databases

ProteinModelPortaliQ08647.
SMRiQ08647. Positions 208-661.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini338 – 582245TRUDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the pseudouridine synthase TruD family.Curated
Contains 1 TRUD domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000063554.
HOGENOMiHOG000203484.
InParanoidiQ08647.
KOiK06176.
OMAiRRNHHSG.
OrthoDBiEOG7NW6JP.

Family and domain databases

InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001656. PsdUridine_synth_TruD.
IPR020119. PsdUridine_synth_TruD_CS.
IPR011760. PsdUridine_synth_TruD_insert.
[Graphical view]
PANTHERiPTHR13326. PTHR13326. 1 hit.
PfamiPF01142. TruD. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 4 hits.
TIGRFAMsiTIGR00094. tRNA_TruD_broad. 1 hit.
PROSITEiPS50984. TRUD. 1 hit.
PS01268. UPF0024. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08647-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSSEATVK RPLDAHVGPS ENAAKKLKIE QRTQADGIHE ADVGITLFLS
60 70 80 90 100
PELPGFRGQI KQRYTDFLVN EIDQEGKVIH LTDKGFKMPK KPQRSKEEVN
110 120 130 140 150
AEKESEAARR QEFNVDPELR NQLVEIFGEE DVLKIESVYR TANKMETAKN
160 170 180 190 200
FEDKSVRTKI HQLLREAFKN ELESVTTDTN TFKIARSNRN SRTNKQEKIN
210 220 230 240 250
QTRDANGVEN WGYGPSKDFI HFTLHKENKD TMEAVNVITK LLRVPSRVIR
260 270 280 290 300
YAGTKDRRAV TCQRVSISKI GLDRLNALNR TLKGMIIGNY NFSDASLNLG
310 320 330 340 350
DLKGNEFVVV IRDVTTGNSE VSLEEIVSNG CKSLSENGFI NYFGMQRFGT
360 370 380 390 400
FSISTHTIGR ELLLSNWKKA AELILSDQDN VLPKSKEARK IWAETKDAAL
410 420 430 440 450
ALKQMPRQCL AENALLYSLS NQRKEEDGTY SENAYYTAIM KIPRNLRTMY
460 470 480 490 500
VHAYQSYVWN SIASKRIELH GLKLVVGDLV IDTSEKSPLI SGIDDEDFDE
510 520 530 540 550
DVREAQFIRA KAVTQEDIDS VKYTMEDVVL PSPGFDVLYP SNEELKQLYV
560 570 580 590 600
DILKADNMDP FNMRRKVRDF SLAGSYRTVI QKPKSLEYRI IHYDDPSQQL
610 620 630 640 650
VNTDLDILNN TRAKESGQKY MKAKLDRYMP DKGGEKTAVV LKFQLGTSAY
660 670
ATMALRELMK LETSRRGDMC DVKENI
Length:676
Mass (Da):77,002
Last modified:November 1, 1996 - v1
Checksum:i4B63F137B781FCCB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75151 Genomic DNA. Translation: CAA99464.1.
BK006948 Genomic DNA. Translation: DAA11011.1.
PIRiS67136.
RefSeqiNP_014886.1. NM_001183662.1.

Genome annotation databases

EnsemblFungiiYOR243C; YOR243C; YOR243C.
GeneIDi854417.
KEGGisce:YOR243C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75151 Genomic DNA. Translation: CAA99464.1.
BK006948 Genomic DNA. Translation: DAA11011.1.
PIRiS67136.
RefSeqiNP_014886.1. NM_001183662.1.

3D structure databases

ProteinModelPortaliQ08647.
SMRiQ08647. Positions 208-661.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34634. 53 interactions.
DIPiDIP-6762N.
IntActiQ08647. 11 interactions.
MINTiMINT-617058.

Proteomic databases

MaxQBiQ08647.
TopDownProteomicsiQ08647.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR243C; YOR243C; YOR243C.
GeneIDi854417.
KEGGisce:YOR243C.

Organism-specific databases

EuPathDBiFungiDB:YOR243C.
SGDiS000005769. PUS7.

Phylogenomic databases

GeneTreeiENSGT00530000063554.
HOGENOMiHOG000203484.
InParanoidiQ08647.
KOiK06176.
OMAiRRNHHSG.
OrthoDBiEOG7NW6JP.

Enzyme and pathway databases

BioCyciYEAST:G3O-33737-MONOMER.
BRENDAi5.4.99.27. 984.
ReactomeiR-SCE-6782315. tRNA modification in the nucleus and cytosol.

Miscellaneous databases

PROiQ08647.

Family and domain databases

InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001656. PsdUridine_synth_TruD.
IPR020119. PsdUridine_synth_TruD_CS.
IPR011760. PsdUridine_synth_TruD_insert.
[Graphical view]
PANTHERiPTHR13326. PTHR13326. 1 hit.
PfamiPF01142. TruD. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 4 hits.
TIGRFAMsiTIGR00094. tRNA_TruD_broad. 1 hit.
PROSITEiPS50984. TRUD. 1 hit.
PS01268. UPF0024. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of a 26.9 kb fragment from chromosome XV of the yeast Saccharomyces cerevisiae."
    Boyer J., Michaux G., Fairhead C., Gaillon L., Dujon B.
    Yeast 12:1575-1586(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine."
    Newby M.I., Greenbaum N.L.
    Nat. Struct. Biol. 9:958-965(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Pseudouridylation (Psi) of U2 snRNA in S. cerevisiae is catalyzed by an RNA-independent mechanism."
    Ma X., Zhao X., Yu Y.T.
    EMBO J. 22:1889-1897(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:Psi-synthase also acting on tRNAs."
    Behm-Ansmant I., Urban A., Ma X., Yu Y.T., Motorin Y., Branlant C.
    RNA 9:1371-1382(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ASP-256.
  9. "Psi35 in the branch site recognition region of U2 small nuclear RNA is important for pre-mRNA splicing in Saccharomyces cerevisiae."
    Yang C., McPheeters D.S., Yu Y.T.
    J. Biol. Chem. 280:6655-6662(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "X-ray structures of U2 snRNA-branchpoint duplexes containing conserved pseudouridines."
    Lin Y., Kielkopf C.L.
    Biochemistry 47:5503-5514(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Different mechanisms for pseudouridine formation in yeast 5S and 5.8S rRNAs."
    Decatur W.A., Schnare M.N.
    Mol. Cell. Biol. 28:3089-3100(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "RNA sequence and two-dimensional structure features required for efficient substrate modification by the Saccharomyces cerevisiae RNA:{Psi}-synthase Pus7p."
    Urban A., Behm-Ansmant I., Branlant C., Motorin Y.
    J. Biol. Chem. 284:5845-5858(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPUS7_YEAST
AccessioniPrimary (citable) accession number: Q08647
Secondary accession number(s): D6W2U5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4150 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.