Reviewed, UniProtKB/Swiss-Prot Q08645 (FOLE_YEAST)

Names and origin

Protein names

Recommended name:
    Folylpolyglutamate synthase
    EC=6.3.2.17
Alternative name(s):
    Folylpoly-gamma-glutamate synthetase
      Short name=FPGS
    Tetrahydrofolylpolyglutamate synthase
      Short name=Tetrahydrofolate synthase

Gene names

Name:	MET7
Synonyms:	MET23
Ordered Locus Names:	YOR241W
ORF Names:	O5248

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conversion of folates to polyglutamate derivatives.
Catalytic activity	ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1).
Pathway	Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.
Subcellular location	Mitochondrion. Cytoplasm. Ref.5
Miscellaneous	Present with 7080 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the folylpolyglutamate synthase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm Mitochondrion
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Ref.5 Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrion Ref.5 Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct tetrahydrofolylpolyglutamate synthase activity Ref.5 Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PRP40	P33203	1	EBI-7058,EBI-701

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 548	548	Folylpolyglutamate synthase		PRO_0000168312
Regions
	Nucleotide binding	126 – 132	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q08645-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: F36811FB4B7E9305	FASTA	548	62,151
	10 20 30 40 50 60 MHKGKKNYPN LITSFRMNLK KIILNHDRFS HPERWKTNAL LRFTFVYIKF LFDLMIIKNP 70 80 90 100 110 120 LRMVGKTYRD AVTALNSLQS NYANIMAIRQ TGDRKNTMTL LEMHEWSRRI GYSASDFNKL 130 140 150 160 170 180 NIVHITGTKG KGSTAAFTSS ILGQYKEQLP RIGLYTSPHL KSVRERIRIN GEPISEEKFA 190 200 210 220 230 240 KYFFEVWDRL DSTTSSLDKF PHMIPGSKPG YFKFLTLLSF HTFIQEDCKS CVYEVGVGGE 250 260 270 280 290 300 LDSTNIIEKP IVCGVTLLGI DHTFMLGDTI EEIAWNKGGI FKSGAPAFTV EKQPPQGLTI 310 320 330 340 350 360 LKERAEERKT TLTEVPPFKQ LENVKLGIAG EFQKSNASLA VMLASEILHT SNILEEKIKC 370 380 390 400 410 420 SSNASIPEKF IIGLQNTKWE GRCQVLEKGK NVWYIDGAHT KDSMVAASTW FRDMVRLSKR 430 440 450 460 470 480 KKILLFNQQS RDANALVNNL YSSVSPEITF DDVIFTTNVT WKSGSYSADL VSMNTSQEDV 490 500 510 520 530 540 EKLKVQESLV KNWNKIDDNR AKTHVTASIE EANELIETLY DEPADIFVTG SLHLVGGLLV VFDRIDVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of a 26.9 kb fragment from chromosome XV of the yeast Saccharomyces cerevisiae." Boyer J., Michaux G., Fairhead C., Gaillon L., Dujon B. Yeast 12:1575-1586(1996) [PubMed: 8972580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Polyglutamylation of folate coenzymes is necessary for methionine biosynthesis and maintenance of intact mitochondrial genome in Saccharomyces cerevisiae." Cherest H., Thomas D., Surdin-Kerjan Y. J. Biol. Chem. 275:14056-14063(2000) [PubMed: 10799479] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Disruption of cytoplasmic and mitochondrial folylpolyglutamate synthetase activity in Saccharomyces cerevisiae." DeSouza L., Shen Y., Bognar A.L. Arch. Biochem. Biophys. 376:299-312(2000) [PubMed: 10775416] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z75149 Genomic DNA. Translation: CAA99462.1. AY692818 Genomic DNA. Translation: AAT92837.1.
PIR	S67134.
RefSeq	NP_014884.1.
3D structure databases
HSSP	HSSP built from PDB template 1FGS based on UniProtKB P15925.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4986N.
IntAct	Q08645. 20 interactions.
Proteomic databases
PeptideAtlas	Q08645.
Genome annotation databases
Ensembl	YOR241W. Saccharomyces cerevisiae. [Contig view]
GeneID	854415.
GenomeReviews	Gene locus YOR241W in contig Y13140_GR.
KEGG	sce:YOR241W.
NMPDR	fig|4932.3.peg.5996.
Organism-specific databases
CYGD	YOR241w.
SGD	S000005767. MET7.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q08645.
OMA	Q08645. GIDHTFM.
Enzyme and pathway databases
BRENDA	6.3.2.17. 250.
Gene expression databases
GermOnline	YOR241W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018109. Folylpolyglutamate_synth_CS. IPR001645. Fpolygl_synthtse. IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
PANTHER	PTHR11136. Fpolygl_synthtse. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01499. folC. 1 hit.
PROSITE	PS01011. FOLYLPOLYGLU_SYNT_1. 1 hit. PS01012. FOLYLPOLYGLU_SYNT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	976613.

Entry information

Entry name

FOLE_YEAST

Accession

Primary (citable) accession number: Q08645

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)