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Protein

Folylpolyglutamate synthase

Gene

MET7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Required for methionine synthesis and maintenance of intact mitochondrial DNA. Involved in telomere maintenance.2 Publications

Catalytic activityi

ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1).2 Publications

Cofactori

a monovalent cationBy similarityNote: A monovalent cation.By similarity

Pathwayi: tetrahydrofolylpolyglutamate biosynthesis

This protein is involved in the pathway tetrahydrofolylpolyglutamate biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolylpolyglutamate biosynthesis and in Cofactor biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi126 – 1327ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • tetrahydrofolylpolyglutamate synthase activity Source: SGD

GO - Biological processi

  • one-carbon metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YOR241W-MONOMER.
ReactomeiR-SCE-196757. Metabolism of folate and pterines.
UniPathwayiUPA00850.

Names & Taxonomyi

Protein namesi
Recommended name:
Folylpolyglutamate synthase (EC:6.3.2.17)
Alternative name(s):
Folylpoly-gamma-glutamate synthetase
Short name:
FPGS
Tetrahydrofolylpolyglutamate synthase
Short name:
Tetrahydrofolate synthase
Gene namesi
Name:MET7
Synonyms:MET23
Ordered Locus Names:YOR241W
ORF Names:O5248
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR241W.
SGDiS000005767. MET7.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • mitochondrial inner membrane Source: UniProtKB-SubCell
  • mitochondrial matrix Source: GO_Central
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Methionine auxotroph. Undetectable levels of folylpolyglutamate synthase activity. Increased rate of loss of mitochondrial DNA. Respiration-deficient. MET7 and FOL3 double mutant cells are not viable even in the presence of methionine and folinic acid. MET7, FOL3 and TUP1 triple disruption mutant is able to germinate on YPGA medium containing thymidylate and to grow although poorly on synthetic medium containing methionine, adenine and thymidylate. Adenine and thymidine auxotroph when grown in the presence of sulfanilamide. Becomes petite under normal growth conditions but can be maintained with a grande phenotype if the strain is TUP1 and all media are supplemented with dTMP. MET7 and GLY1 double mutant is auxotrophic for glycine when grown on glucose but prototrophic when grown on glycerol. MET7 and SER1 double mutant cannot use glycine to suppress serine auxotrophy. MET7 and SHM2 double mutant is nonviable.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 548548Folylpolyglutamate synthasePRO_0000168312Add
BLAST

Proteomic databases

MaxQBiQ08645.
TopDownProteomicsiQ08645.

PTM databases

iPTMnetiQ08645.

Interactioni

Protein-protein interaction databases

BioGridi34632. 112 interactions.
DIPiDIP-4986N.
IntActiQ08645. 9 interactions.
MINTiMINT-513781.

Structurei

3D structure databases

ProteinModelPortaliQ08645.
SMRiQ08645. Positions 88-404.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the folylpolyglutamate synthase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000016526.
HOGENOMiHOG000181278.
InParanoidiQ08645.
KOiK01930.
OMAiEFAIPEM.
OrthoDBiEOG092C3XJ8.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.90.190.20. 1 hit.
InterProiIPR001645. Folylpolyglutamate_synth.
IPR018109. Folylpolyglutamate_synth_CS.
IPR023600. Folylpolyglutamate_synth_euk.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
[Graphical view]
PANTHERiPTHR11136. PTHR11136. 1 hit.
PTHR11136:SF5. PTHR11136:SF5. 1 hit.
PIRSFiPIRSF038895. FPGS. 1 hit.
SUPFAMiSSF53244. SSF53244. 2 hits.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01499. folC. 1 hit.
PROSITEiPS01011. FOLYLPOLYGLU_SYNT_1. 1 hit.
PS01012. FOLYLPOLYGLU_SYNT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08645-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHKGKKNYPN LITSFRMNLK KIILNHDRFS HPERWKTNAL LRFTFVYIKF
60 70 80 90 100
LFDLMIIKNP LRMVGKTYRD AVTALNSLQS NYANIMAIRQ TGDRKNTMTL
110 120 130 140 150
LEMHEWSRRI GYSASDFNKL NIVHITGTKG KGSTAAFTSS ILGQYKEQLP
160 170 180 190 200
RIGLYTSPHL KSVRERIRIN GEPISEEKFA KYFFEVWDRL DSTTSSLDKF
210 220 230 240 250
PHMIPGSKPG YFKFLTLLSF HTFIQEDCKS CVYEVGVGGE LDSTNIIEKP
260 270 280 290 300
IVCGVTLLGI DHTFMLGDTI EEIAWNKGGI FKSGAPAFTV EKQPPQGLTI
310 320 330 340 350
LKERAEERKT TLTEVPPFKQ LENVKLGIAG EFQKSNASLA VMLASEILHT
360 370 380 390 400
SNILEEKIKC SSNASIPEKF IIGLQNTKWE GRCQVLEKGK NVWYIDGAHT
410 420 430 440 450
KDSMVAASTW FRDMVRLSKR KKILLFNQQS RDANALVNNL YSSVSPEITF
460 470 480 490 500
DDVIFTTNVT WKSGSYSADL VSMNTSQEDV EKLKVQESLV KNWNKIDDNR
510 520 530 540
AKTHVTASIE EANELIETLY DEPADIFVTG SLHLVGGLLV VFDRIDVK
Length:548
Mass (Da):62,151
Last modified:November 1, 1997 - v1
Checksum:iF36811FB4B7E9305
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75149 Genomic DNA. Translation: CAA99462.1.
AY692818 Genomic DNA. Translation: AAT92837.1.
BK006948 Genomic DNA. Translation: DAA11009.1.
PIRiS67134.
RefSeqiNP_014884.1. NM_001183660.1.

Genome annotation databases

EnsemblFungiiYOR241W; YOR241W; YOR241W.
GeneIDi854415.
KEGGisce:YOR241W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75149 Genomic DNA. Translation: CAA99462.1.
AY692818 Genomic DNA. Translation: AAT92837.1.
BK006948 Genomic DNA. Translation: DAA11009.1.
PIRiS67134.
RefSeqiNP_014884.1. NM_001183660.1.

3D structure databases

ProteinModelPortaliQ08645.
SMRiQ08645. Positions 88-404.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34632. 112 interactions.
DIPiDIP-4986N.
IntActiQ08645. 9 interactions.
MINTiMINT-513781.

PTM databases

iPTMnetiQ08645.

Proteomic databases

MaxQBiQ08645.
TopDownProteomicsiQ08645.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR241W; YOR241W; YOR241W.
GeneIDi854415.
KEGGisce:YOR241W.

Organism-specific databases

EuPathDBiFungiDB:YOR241W.
SGDiS000005767. MET7.

Phylogenomic databases

GeneTreeiENSGT00390000016526.
HOGENOMiHOG000181278.
InParanoidiQ08645.
KOiK01930.
OMAiEFAIPEM.
OrthoDBiEOG092C3XJ8.

Enzyme and pathway databases

UniPathwayiUPA00850.
BioCyciYEAST:YOR241W-MONOMER.
ReactomeiR-SCE-196757. Metabolism of folate and pterines.

Miscellaneous databases

PROiQ08645.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.90.190.20. 1 hit.
InterProiIPR001645. Folylpolyglutamate_synth.
IPR018109. Folylpolyglutamate_synth_CS.
IPR023600. Folylpolyglutamate_synth_euk.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
[Graphical view]
PANTHERiPTHR11136. PTHR11136. 1 hit.
PTHR11136:SF5. PTHR11136:SF5. 1 hit.
PIRSFiPIRSF038895. FPGS. 1 hit.
SUPFAMiSSF53244. SSF53244. 2 hits.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01499. folC. 1 hit.
PROSITEiPS01011. FOLYLPOLYGLU_SYNT_1. 1 hit.
PS01012. FOLYLPOLYGLU_SYNT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFOLE_YEAST
AccessioniPrimary (citable) accession number: Q08645
Secondary accession number(s): D6W2U3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7080 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.