Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q08645 (FOLE_YEAST)

Last modified June 16, 2009. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Folylpolyglutamate synthase
    EC=6.3.2.17
Alternative name(s):
    Folylpoly-gamma-glutamate synthetase
      Short name=FPGS
    Tetrahydrofolylpolyglutamate synthase
      Short name=Tetrahydrofolate synthase
Gene names
Name: MET7
Synonyms: MET23
Ordered Locus Names: YOR241W
ORF Names: O5248
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length548 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Conversion of folates to polyglutamate derivatives.

Catalytic activity

ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1).

Pathway

Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.

Subcellular location

Mitochondrion. Cytoplasm. Ref.5

Miscellaneous

Present with 7080 molecules/cell in log phase SD medium. Ref.6

Sequence similarities

Belongs to the folylpolyglutamate synthase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PRP40P332031EBI-7058,EBI-701

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 548548Folylpolyglutamate synthase
PRO_0000168312

Regions

Nucleotide binding126 – 1327ATP Potential

Sequences

Sequence LengthMass (Da)Tools
Q08645-1 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: F36811FB4B7E9305

FASTA54862,151
        10         20         30         40         50         60 
MHKGKKNYPN LITSFRMNLK KIILNHDRFS HPERWKTNAL LRFTFVYIKF LFDLMIIKNP 

        70         80         90        100        110        120 
LRMVGKTYRD AVTALNSLQS NYANIMAIRQ TGDRKNTMTL LEMHEWSRRI GYSASDFNKL 

       130        140        150        160        170        180 
NIVHITGTKG KGSTAAFTSS ILGQYKEQLP RIGLYTSPHL KSVRERIRIN GEPISEEKFA 

       190        200        210        220        230        240 
KYFFEVWDRL DSTTSSLDKF PHMIPGSKPG YFKFLTLLSF HTFIQEDCKS CVYEVGVGGE 

       250        260        270        280        290        300 
LDSTNIIEKP IVCGVTLLGI DHTFMLGDTI EEIAWNKGGI FKSGAPAFTV EKQPPQGLTI 

       310        320        330        340        350        360 
LKERAEERKT TLTEVPPFKQ LENVKLGIAG EFQKSNASLA VMLASEILHT SNILEEKIKC 

       370        380        390        400        410        420 
SSNASIPEKF IIGLQNTKWE GRCQVLEKGK NVWYIDGAHT KDSMVAASTW FRDMVRLSKR 

       430        440        450        460        470        480 
KKILLFNQQS RDANALVNNL YSSVSPEITF DDVIFTTNVT WKSGSYSADL VSMNTSQEDV 

       490        500        510        520        530        540 
EKLKVQESLV KNWNKIDDNR AKTHVTASIE EANELIETLY DEPADIFVTG SLHLVGGLLV 


VFDRIDVK 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of a 26.9 kb fragment from chromosome XV of the yeast Saccharomyces cerevisiae."
Boyer J., Michaux G., Fairhead C., Gaillon L., Dujon B.
Yeast 12:1575-1586(1996) [PubMed: 8972580] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed: 9169874] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Polyglutamylation of folate coenzymes is necessary for methionine biosynthesis and maintenance of intact mitochondrial genome in Saccharomyces cerevisiae."
Cherest H., Thomas D., Surdin-Kerjan Y.
J. Biol. Chem. 275:14056-14063(2000) [PubMed: 10799479] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Disruption of cytoplasmic and mitochondrial folylpolyglutamate synthetase activity in Saccharomyces cerevisiae."
DeSouza L., Shen Y., Bognar A.L.
Arch. Biochem. Biophys. 376:299-312(2000) [PubMed: 10775416] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

Z75149 Genomic DNA. Translation: CAA99462.1.
AY692818 Genomic DNA. Translation: AAT92837.1.
PIRS67134.
RefSeqNP_014884.1.

3D structure databases

HSSPHSSP built from PDB template 1FGS based on UniProtKB P15925.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:4986N.
IntActQ08645. 20 interactions.

Proteomic databases

PeptideAtlasQ08645.

Genome annotation databases

EnsemblYOR241W. Saccharomyces cerevisiae. [Contig view]
GeneID854415.
GenomeReviewsGene locus YOR241W in contig Y13140_GR.
KEGGsce:YOR241W.
NMPDRfig|4932.3.peg.5996.

Organism-specific databases

CYGDYOR241w.
SGDS000005767. MET7.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMQ08645.
OMAQ08645. GIDHTFM.

Enzyme and pathway databases

BRENDA6.3.2.17. 250.

Gene expression databases

GermOnlineYOR241W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR018109. Folylpolyglutamate_synth_CS.
IPR001645. Fpolygl_synthtse.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
PANTHERPTHR11136. Fpolygl_synthtse. 1 hit.
PfamPF02875. Mur_ligase_C. 1 hit.
[Graphical view]
TIGRFAMsTIGR01499. folC. 1 hit.
PROSITEPS01011. FOLYLPOLYGLU_SYNT_1. 1 hit.
PS01012. FOLYLPOLYGLU_SYNT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio976613.

Entry information

Entry nameFOLE_YEAST
AccessionPrimary (citable) accession number: Q08645
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 16, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents