Q08645 (FOLE_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 108.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Folylpolyglutamate synthase EC=6.3.2.17 Alternative name(s): Folylpoly-gamma-glutamate synthetase Short name=FPGS Tetrahydrofolylpolyglutamate synthase Short name=Tetrahydrofolate synthase | ||||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ||||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 548 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Required for methionine synthesis and maintenance of intact mitochondrial DNA. Involved in telomere maintenance. Ref.5 Ref.8 |
| Catalytic activity | ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1). Ref.5 Ref.6 |
| Cofactor | A monovalent cation By similarity. |
| Pathway | Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis. |
| Subcellular location | Mitochondrion. Mitochondrion inner membrane By similarity. Mitochondrion matrix By similarity. Cytoplasm Ref.6. |
| Disruption phenotype | Methionine auxotroph. Undetectable levels of folylpolyglutamate synthase activity. Increased rate of loss of mitochondrial DNA. Respiration-deficient. In combination with deletion of FOL3, the cells are not viable even in the presence of methionine and folinic acid. MET7, FOL3 and TUP1 triple disruption mutant is able to germinate on YPGA medium containing thymidylate and to grow although poorly on synthetic medium containing methionine, adenine and thymidylate. Adenine and thymidine auxotroph when grown in the presence of sulfanilamide. Becomes petite under normal growth conditions but can be maintained with a grande phenotype if the strain is TUP1 and all media are supplemented with dTMP. MET7 GLY1 double mutant is auxotrophic for glycine when grown on glucose but prototrophic when grown on glycerol. MET7 SER1 double mutant cannot use glycine to suppress serine auxotrophy. MET7 SHM2 double mutant is nonviable. Ref.5 Ref.6 |
| Miscellaneous | Present with 7080 molecules/cell in log phase SD medium. Ref.7 |
| Sequence similarities | Belongs to the folylpolyglutamate synthase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | One-carbon metabolism |
| Cellular component | Cytoplasm Membrane Mitochondrion Mitochondrion inner membrane |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Ligase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | one-carbon metabolic process Inferred from mutant phenotype Ref.6. Source: SGD |
| Cellular component | mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial matrixInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetrahydrofolylpolyglutamate synthase activityInferred from direct assay Ref.6. Source: SGD |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequence and analysis of a 26.9 kb fragment from chromosome XV of the yeast Saccharomyces cerevisiae." Boyer J., Michaux G., Fairhead C., Gaillon L., Dujon B. Yeast 12:1575-1586(1996) [PubMed: 8972580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K.Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [3] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [4] | "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J.Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [5] | "Polyglutamylation of folate coenzymes is necessary for methionine biosynthesis and maintenance of intact mitochondrial genome in Saccharomyces cerevisiae." Cherest H., Thomas D., Surdin-Kerjan Y. J. Biol. Chem. 275:14056-14063(2000) [PubMed: 10799479] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE. |
| [6] | "Disruption of cytoplasmic and mitochondrial folylpolyglutamate synthetase activity in Saccharomyces cerevisiae." DeSouza L., Shen Y., Bognar A.L. Arch. Biochem. Biophys. 376:299-312(2000) [PubMed: 10775416] [Abstract] Cited for: CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE. |
| [7] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [8] | "A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length." Askree S.H., Yehuda T., Smolikov S., Gurevich R., Hawk J., Coker C., Krauskopf A., Kupiec M., McEachern M.J. Proc. Natl. Acad. Sci. U.S.A. 101:8658-8663(2004) [PubMed: 15161972] [Abstract] Cited for: FUNCTION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Z75149 Genomic DNA. Translation: CAA99462.1. AY692818 Genomic DNA. Translation: AAT92837.1. BK006948 Genomic DNA. Translation: DAA11009.1. |
| PIR | S67134. |
| RefSeq | NP_014884.1. NM_001183660.1. |
3D structure databases | |
| ProteinModelPortal | Q08645. |
| SMR | Q08645. Positions 66-546. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-4986N. |
| IntAct | Q08645. 9 interactions. |
| MINT | MINT-513781. |
| STRING | Q08645. |
Proteomic databases | |
| PeptideAtlas | Q08645. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YOR241W; YOR241W; YOR241W. |
| GeneID | 854415. |
| KEGG | sce:YOR241W. |
| NMPDR | fig|4932.3.peg.5996. |
Organism-specific databases | |
| CYGD | YOR241w. |
| SGD | S000005767. MET7. |
Phylogenomic databases | |
| eggNOG | fuNOG04751. |
| GeneTree | EFGT00050000004649. |
| HOGENOM | HBG744947. |
| OMA | STGNADQ. |
| OrthoDB | EOG425928. |
Gene expression databases | |
| ArrayExpress | Q08645. |
| Genevestigator | Q08645. |
| GermOnline | YOR241W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR001645. Folylpolyglutamate_synth. IPR018109. Folylpolyglutamate_synth_CS. IPR023600. Folylpolyglutamate_synth_euk. IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. [Graphical view] |
| Gene3D | G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. |
| KO | K01930. |
| PANTHER | PTHR11136. Fpolygl_synthtse. 1 hit. |
| PIRSF | PIRSF038895. FPGS. 1 hit. |
| SUPFAM | SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit. |
| TIGRFAMs | TIGR01499. FolC. 1 hit. |
| PROSITE | PS01011. FOLYLPOLYGLU_SYNT_1. 1 hit. PS01012. FOLYLPOLYGLU_SYNT_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 976613. |
Entry information
| Entry name | FOLE_YEAST | ||||||||
| Accession | Primary (citable) accession number: Q08645 Secondary accession number(s): D6W2U3 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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