Reviewed, UniProtKB/Swiss-Prot Q08641 (AB140_YEAST)

Names and origin

Protein names

Recommended name:
    Uncharacterized methyltransferase ABP140
    EC=2.1.1.-
Alternative name(s):
    140 kDa actin-binding protein

Gene names

Name:	ABP140
Ordered Locus Names:	YOR239W/YOR240W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	628 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Potential methyltransferase By similarity. Binds F-actin and shows weak F-actin cross-linking activity.
Subcellular location	Cytoplasm. Cytoplasm › cytoskeleton. Note: Cytoplasmic and cortical cytoskeleton. Ref.3
Sequence similarities	Belongs to the methyltransferase superfamily. METL family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Ribosomal frameshifting
Ligand	Actin-binding
Molecular function	Methyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	actin cytoskeleton organization Ref.3 Inferred from direct assay. Source: SGD
Cellular component	actin filament Ref.3 Inferred from direct assay. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell mating projection tip Inferred from direct assay. Source: SGD
Molecular function	actin filament binding Ref.3 Inferred from direct assay. Source: SGD methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding, bridging Ref.3 Inferred from direct assay. Source: SGD
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by ribosomal frameshifting. [Select]

Isoform 1 (identifier: Q08641-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Produced by ribosomal frameshifting between codon Leu-277 and Gly-278.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 628	627	Uncharacterized methyltransferase ABP140		PRO_0000204458
Amino acid modifications
	Modified residue	93	1	Phosphoserine Ref.4 Ref.7
	Modified residue	150	1	Phosphothreonine Ref.7
	Modified residue	321	1	Phosphoserine Ref.4 Ref.7 Ref.6
	Modified residue	326	1	Phosphoserine Ref.4 Ref.7 Ref.6 Ref.5
	Modified residue	328	1	Phosphothreonine Ref.5
	Modified residue	331	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 74F9CA30BC5BCABF	FASTA	628	71,486
	10 20 30 40 50 60 MGVADLIKKF ESISKEEGDA TVDTNSSSKP LKSNDETKEL HQQESTAVPQ EVDVNEEFEN 70 80 90 100 110 120 EPETINSSRT AEKPLETNLP KPETNEEDEE EGSMSENKIY SKGENADINV NDFQEYKEME 130 140 150 160 170 180 NTGAEVLASS VEESDAIQEG VAEETEGIAT PKQKENEKND ESEEESANNA SEPAEEYSQS 190 200 210 220 230 240 EEDADIEQSN GKETENAENA SQQANDGSTS TTTSKNKKKK NKKKNKKKRN GNVNTNANVD 250 260 270 280 290 300 DSTKTGENDD TTGDTTSSTT SAIQEVNDLE VVDDSCLGID QQHNREHLKA LTQDVKEETL 310 320 330 340 350 360 ENIAHEGRGD NTGDQNAVEK SDFEKSDTEG SRIGRDLPFE FGKRNLTEES DVWDHNAWDN 370 380 390 400 410 420 VEWGEEQVQQ AEEKIKEQFK HPVPEFDKKL YNENPARYWD IFYKNNKENF FKDRKWLQIE 430 440 450 460 470 480 FPILYASTRK DAEPVTIFEI GCGAGNTFFP ILKDNENENL RIIAADFAPR AVELVKNSEQ 490 500 510 520 530 540 FNPKYGHATV WDLANPDGNL PDGVEPHSVD IAVMIFVFSA LAPNQWDQAM DNLHKILKPG 550 560 570 580 590 600 GKIIFRDYGA YDLTQVRFKK NRILEENFYV RGDGTRVYFF SEEKLREIFT KKYFLENKIG 610 620 TDRRLLVNRK RQLKMYRCWV QAVFDVPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of a 26.9 kb fragment from chromosome XV of the yeast Saccharomyces cerevisiae." Boyer J., Michaux G., Fairhead C., Gaillon L., Dujon B. Yeast 12:1575-1586(1996) [PubMed: 8972580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Isolation and characterization of a novel actin filament-binding protein from Saccharomyces cerevisiae." Asakura T., Sasaki T., Nagano F., Satoh A., Obaishi H., Nishioka H., Imamura H., Hotta K., Tanaka K., Nakanishi H., Takai Y. Oncogene 16:121-130(1998) [PubMed: 9467951] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 2-13; 39-73; 103-116; 245-287; 561-575 AND 599-609, FUNCTION, SUBCELLULAR LOCATION. Strain: BJ5457.
[4]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-93; SER-321 AND SER-326, MASS SPECTROMETRY.
[5]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326; THR-328 AND SER-331, MASS SPECTROMETRY.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-321 AND SER-326, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-93; THR-150; SER-321 AND SER-326, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z75147 Genomic DNA. Translation: CAA99460.1. Sequence problems. Z75147 Genomic DNA. Translation: CAA99461.1. Sequence problems.
RefSeq	NP_014882.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4117N.
IntAct	Q08641. 31 interactions.
STRING	Q08641.
Proteomic databases
PeptideAtlas	Q08641.
Genome annotation databases
Ensembl	YOR239W; YOR239W; YOR239W; Saccharomyces cerevisiae. [Genome view]
GeneID	854414.
KEGG	sce:YOR239W.
NMPDR	fig|4932.3.peg.5995.
Organism-specific databases
CYGD	YOR239w.
SGD	S000005765. ABP140.
Phylogenomic databases
HOGENOM	Q08641.
OMA	HEANNIV
OrthoDB	EOG9615MM
Gene expression databases
ArrayExpress	Q08641.
Genevestigator	Q08641.
GermOnline	YOR239W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013217. Methyltransf_12. [Graphical view]
Pfam	PF08242. Methyltransf_12. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	976610.

Entry information

Entry name

AB140_YEAST

Accession

Primary (citable) accession number: Q08641
Secondary accession number(s): Q08644

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)