Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA(Thr) (cytosine(32)-N(3))-methyltransferase

Gene

ABP140

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that mediates 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA(Thr) and tRNA(Ser). Binds F-actin and shows weak F-actin cross-linking activity.3 Publications

Miscellaneous

N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift (PubMed:9467951). ABP140 mRNA translation follows a cotranslational transport: mRNA is transported to the distal pole of the mother cell, independently of the SHE machinery, and follows a translational coupling, in which ABP140 mRNA is tethered to actin cables via its nascent protein product and is transported to the distal pole by actin retrograde flow (PubMed:21792172).2 Publications

Catalytic activityi

S-adenosyl-L-methionine + cytosine(32) in tRNA(Thr) = S-adenosyl-L-homocysteine + N3-methylcytosine(32) in tRNA(Thr).2 Publications
S-adenosyl-L-methionine + cytosine(32) in tRNA(Ser) = S-adenosyl-L-homocysteine + N3-methylcytosine(32) in tRNA(Ser).2 Publications

GO - Molecular functioni

  • actin filament binding Source: SGD
  • protein binding, bridging Source: SGD
  • tRNA (cytosine-3-)-methyltransferase activity Source: SGD

GO - Biological processi

  • actin filament bundle assembly Source: SGD
  • tRNA methylation Source: SGD

Keywordsi

Molecular functionActin-binding, Methyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-33735-MONOMER
BRENDAi2.1.1.268 984

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Thr) (cytosine(32)-N(3))-methyltransferase (EC:2.1.1.2682 Publications)
Alternative name(s):
Actin-binding protein of 140 kDa
tRNA methyltransferase of 140 kDa
Gene namesi
Name:ABP140
Synonyms:TRM140
Ordered Locus Names:YOR239W
ORF Names:YOR240W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR239W
SGDiS000005765 ABP140

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002044582 – 628tRNA(Thr) (cytosine(32)-N(3))-methyltransferaseAdd BLAST627

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei93PhosphoserineCombined sources1
Modified residuei150PhosphothreonineCombined sources1
Modified residuei321PhosphoserineCombined sources1
Modified residuei326PhosphoserineCombined sources1
Modified residuei347PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08641
PaxDbiQ08641
PRIDEiQ08641

PTM databases

iPTMnetiQ08641

Interactioni

GO - Molecular functioni

  • actin filament binding Source: SGD
  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi34631, 35 interactors
DIPiDIP-4117N
IntActiQ08641, 27 interactors
STRINGi4932.YOR239W

Structurei

3D structure databases

ProteinModelPortaliQ08641
SMRiQ08641
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00520000055554
HOGENOMiHOG000033747
InParanoidiQ08641
KOiK17053
OMAiHEANNIV
OrthoDBiEOG092C3LCG

Family and domain databases

InterProiView protein in InterPro
IPR013217 Methyltransf_12
IPR026113 MeTrfase
IPR029063 SAM-dependent_MTases
PANTHERiPTHR22809 PTHR22809, 1 hit
PfamiView protein in Pfam
PF08242 Methyltransf_12, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by ribosomal frameshifting. AlignAdd to basket

Isoform 1 (identifier: Q08641-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGVADLIKKF ESISKEEGDA TVDTNSSSKP LKSNDETKEL HQQESTAVPQ
60 70 80 90 100
EVDVNEEFEN EPETINSSRT AEKPLETNLP KPETNEEDEE EGSMSENKIY
110 120 130 140 150
SKGENADINV NDFQEYKEME NTGAEVLASS VEESDAIQEG VAEETEGIAT
160 170 180 190 200
PKQKENEKND ESEEESANNA SEPAEEYSQS EEDADIEQSN GKETENAENA
210 220 230 240 250
SQQANDGSTS TTTSKNKKKK NKKKNKKKRN GNVNTNANVD DSTKTGENDD
260 270 280 290 300
TTGDTTSSTT SAIQEVNDLE VVDDSCLGID QQHNREHLKA LTQDVKEETL
310 320 330 340 350
ENIAHEGRGD NTGDQNAVEK SDFEKSDTEG SRIGRDLPFE FGKRNLTEES
360 370 380 390 400
DVWDHNAWDN VEWGEEQVQQ AEEKIKEQFK HPVPEFDKKL YNENPARYWD
410 420 430 440 450
IFYKNNKENF FKDRKWLQIE FPILYASTRK DAEPVTIFEI GCGAGNTFFP
460 470 480 490 500
ILKDNENENL RIIAADFAPR AVELVKNSEQ FNPKYGHATV WDLANPDGNL
510 520 530 540 550
PDGVEPHSVD IAVMIFVFSA LAPNQWDQAM DNLHKILKPG GKIIFRDYGA
560 570 580 590 600
YDLTQVRFKK NRILEENFYV RGDGTRVYFF SEEKLREIFT KKYFLENKIG
610 620
TDRRLLVNRK RQLKMYRCWV QAVFDVPQ
Note: Produced by ribosomal frameshifting between codon Leu-277 and Gly-278.
Length:628
Mass (Da):71,486
Last modified:January 23, 2007 - v3
Checksum:i74F9CA30BC5BCABF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75147 Genomic DNA Translation: CAA99460.1 Sequence problems.
Z75147 Genomic DNA Translation: CAA99461.1 Sequence problems.
BK006948 Genomic DNA Translation: DAA11008.1
RefSeqiNP_014882.4, NM_001183658.6 [Q08641-1]

Genome annotation databases

EnsemblFungiiYOR239W; YOR239W; YOR239W [Q08641-1]
GeneIDi854414
KEGGisce:YOR239W

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Similar proteinsi

Entry informationi

Entry nameiAB140_YEAST
AccessioniPrimary (citable) accession number: Q08641
Secondary accession number(s): D6W2U2, Q08644
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 155 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health