Reviewed, UniProtKB/Swiss-Prot Q08639 (TFDP1_MOUSE)

Names and origin

Protein names

Recommended name:
    Transcription factor Dp-1
Alternative name(s):
    E2F dimerization partner 1
    DRTF1-polypeptide 1

Gene names

Name:

Tfdp1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1/DP complex appears to mediate both cell proliferation and apoptosis.
Subunit structure	Component of the E2F/DP transcription factor complex. Forms heterodimers with E2F family members. The complex can interact with hypophosphorylated retinoblastoma protein RB1 and related proteins (RBL1 and RBL2) that inhibit the E2F transactivation domain. This repression involves recruitment of histone deacetylase (HDAC). During the cell cycle, from mid-to-late G1 phase, RB family members become phosphorylated, detach from the DRTF1/E2F complex to render E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB protein, thus releasing the active complex. Part of the E2F6.com-1 complex in G0 phase which is composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2 By similarity.
Subcellular location	Nucleus.
Developmental stage	In the intestinal epithelium, first expressed in undifferentiated and mesenchymal tissues, levels increasing by 12.5 dpc in the epithelial compartment. With epithelial differentiation at 15.5 dpc, Its expression increases substantially in the intervillus epithelium with lower levels in the mesenchyme. At later stages, expression continues in the intervillus epithelium. Also found at lower levels in the developing villi. In the developing brain, highest levels found between 11.5 and 13.5 dpc in the ventricular region. In the developing retina, it is expressed both in retinoblast and ganglion cell layers from 14.5 dpc to 6 days after birth. In other developing tissues, its expression is highest in the thymus. Also present in kidney, lung, liver, heart and chondrocytes. Weakly expressed in skeletal muscle and absent from choroid plexus. Ref.4 Ref.5
Post-translational modification	Phosphorylation by E2F-1-bound cyclin A-CDK2, in the S phase, inhibits E2F-mediated DNA binding and transactivation.
Sequence similarities	Belongs to the E2F/DP family.

Ontologies

Keywords
Biological process	Cell cycle Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	S phase of mitotic cell cycle Inferred from mutant phenotype. Source: MGI apoptosis Inferred from direct assay. Source: MGI epidermis development Inferred from mutant phenotype. Source: MGI positive regulation of transcription, DNA-dependent Inferred from direct assay. Source: MGI transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	transcription factor complex Inferred from direct assay. Source: MGI
Molecular function	protein binding Inferred from physical interaction. Source: MGI transcription activator activity Inferred from direct assay. Source: MGI transcription factor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	Transcription factor Dp-1		PRO_0000219476
Regions
	DNA binding	113 – 195	83	Potential
	Region	204 – 277	74	Dimerization Potential
	Region	214 – 246	33	DCB1
	Region	259 – 315	57	DCB2
	Motif	161 – 195	35	DEF box
	Compositional bias	250 – 253	4	Poly-Pro
	Compositional bias	395 – 410	16	Asp/Glu-rich (acidic; NCB domain)
Amino acid modifications
	Modified residue	23	1	Phosphoserine; by CDK2 Potential
	Modified residue	183	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q08639-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FEEA090C781071B9	FASTA	410	45,231
	10 20 30 40 50 60 MAKDASLIEA NGELKVFIDQ NLSPGKGVVS LVAVHPSTVN TLGKQLLPKT FGQSNVNITQ 70 80 90 100 110 120 QVVIGTPQRP AASNTIVVGS PHTPNTHFVS QNQTSDSSPW SAGKRNRKGE KNGKGLRHFS 130 140 150 160 170 180 MKVCEKVQRK GTTSYNEVAD ELVAEFSAAD NHILPNESAY DQKNIRRRVY DALNVLMAMN 190 200 210 220 230 240 IISKEKKEIK WIGLPTNSAQ ECQNLEVERQ RRLERIKQKQ SQLQELILQQ IAFKNLVQRN 250 260 270 280 290 300 RQAEQQARRP PPPNSVIHLP FIIVNTSRKT VIDCSISNDK FEYLFNFDNT FEIHDDIEVL 310 320 330 340 350 360 KRMGMACGLE SGNCSAEDLK VARSLVPKAL EPYVTEMAQG SIGGVFVTTT GSTSNGTRLS 370 380 390 400 410 ASDLSNGADG MLATSSNGSQ YSGSRVETPV SYVGEDDDDD DDFNENDEED

References

[1]	"A new component of the transcription factor DRTF1/E2F." Girling R., Partridge J.F., Bandara L.R., Burden N., Totty N.F., Hsuan J.J., La Thangue N.B. Nature 362:83-87(1993) [PubMed: 8446173] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"A new component of the transcription factor DRTF1/E2F." Girling R., Partridge J.F., Bandara L.R., Burden N., Totty N.F., Hsuan J.J., La Thangue N.B. Nature 365:468-468(1993) [PubMed: 8413592] [Abstract] Cited for: SEQUENCE REVISION TO 388-410.
[3]	"Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation." Helin K., Wu C.-L., Fattaey A.R., Lees J.A., Dynlacht B.D., Ngwu C., Harlow E. Genes Dev. 7:1850-1861(1993) [PubMed: 8405995] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Expression patterns of the E2F family of transcription factors during murine epithelial development." Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A. Cell Growth Differ. 8:553-563(1997) [PubMed: 9149906] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[5]	"Expression patterns of the E2F family of transcription factors during mouse nervous system development." Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A. Mech. Dev. 66:13-25(1997) [PubMed: 9376316] [Abstract] Cited for: DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X72310 mRNA. Translation: CAA51056.1.
IPI	IPI00122992.
PIR	B48585.
RefSeq	NP_033387.1.
UniGene	Mm.925
3D structure databases
HSSP	HSSP built from PDB template 1CF7 based on UniProtKB Q14188.
SMR	Q08639. Positions 113-195, 199-346.
ModBase	Search...
PTM databases
PhosphoSite	Q08639.
Proteomic databases
PRIDE	Q08639.
Genome annotation databases
Ensembl	ENSMUSG00000038482. Mus musculus. [Contig view]
GeneID	21781.
KEGG	mmu:21781.
Organism-specific databases
MGI	MGI:101934. Tfdp1.
Phylogenomic databases
HOGENOM	Q08639.
HOVERGEN	Q08639.
OMA	Q08639. PHTPSTH.
Gene expression databases
ArrayExpress	Q08639.
Bgee	Q08639.
CleanEx	MM_TFDP1.
GermOnline	ENSMUSG00000038482. Mus musculus.
Family and domain databases
InterPro	IPR003316. E2F_TDP. IPR014889. Transc_factor_DP_C. IPR016556. Transcription_factor_DP_sub. IPR015648. Transcrpt_fac_DP. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
PANTHER	PTHR12548. DP. 1 hit.
Pfam	PF08781. DP. 1 hit. PF02319. E2F_TDP. 1 hit. [Graphical view]
PIRSF	PIRSF009404. Transcription_factor_DP. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	301122.
SOURCE	Search...

Entry information

Entry name

TFDP1_MOUSE

Accession

Primary (citable) accession number: Q08639

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)