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Protein

Transcription factor Dp-1

Gene

Tfdp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The E2F1:DP complex appears to mediate both cell proliferation and apoptosis. Blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters (PubMed:20176812).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi113 – 19583Sequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • anoikis Source: MGI
  • epidermis development Source: MGI
  • mitotic cell cycle Source: InterPro
  • negative regulation of fat cell proliferation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: MGI
  • regulation of DNA biosynthetic process Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-113510. E2F mediated regulation of DNA replication.
R-MMU-1538133. G0 and Early G1.
R-MMU-2173796. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
R-MMU-69205. G1/S-Specific Transcription.
R-MMU-69231. Cyclin D associated events in G1.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor Dp-1
Alternative name(s):
DRTF1-polypeptide 1
E2F dimerization partner 1
Gene namesi
Name:Tfdp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:101934. Tfdp1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: MGI
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Transcription factor Dp-1PRO_0000219476Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei3 – 31N6-acetyllysineBy similarity
Modified residuei23 – 231PhosphoserineCombined sources

Post-translational modificationi

Phosphorylation by E2F1-bound cyclin A-CDK2, in the S phase, inhibits E2F-mediated DNA binding and transactivation.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ08639.
PaxDbiQ08639.
PRIDEiQ08639.

PTM databases

PhosphoSiteiQ08639.

Expressioni

Developmental stagei

In the intestinal epithelium, first expressed in undifferentiated and mesenchymal tissues, levels increasing by 12.5 dpc in the epithelial compartment. With epithelial differentiation at 15.5 dpc, Its expression increases substantially in the intervillus epithelium with lower levels in the mesenchyme. At later stages, expression continues in the intervillus epithelium. Also found at lower levels in the developing villi. In the developing brain, highest levels found between 11.5 and 13.5 dpc in the ventricular region. In the developing retina, it is expressed both in retinoblast and ganglion cell layers from 14.5 dpc to 6 days after birth. In other developing tissues, its expression is highest in the thymus. Also present in kidney, lung, liver, heart and chondrocytes. Weakly expressed in skeletal muscle and absent from choroid plexus.2 Publications

Gene expression databases

BgeeiQ08639.
CleanExiMM_TFDP1.
ExpressionAtlasiQ08639. baseline and differential.
GenevisibleiQ08639. MM.

Interactioni

Subunit structurei

Component of the E2F:DP transcription factor complex. Forms heterodimers with E2F family members. The complex can interact with hypophosphorylated retinoblastoma protein RB1 and related proteins (RBL1 and RBL2) that inhibit the E2F transactivation domain. This repression involves recruitment of histone deacetylase (HDAC). During the cell cycle, from mid-to-late G1 phase, RB family members become phosphorylated, detach from the DRTF1/E2F complex to render E2F transcriptionally active. Part of the E2F6.com-1 complex in G0 phase is composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 YAF2. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. The complex TFDP1:E2F1 interacts with CEBPA; the interaction prevents CEBPA binding to target gene promoters and represses its transcriptional activity (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Dvl1P511413EBI-1216575,EBI-1538407

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204138. 7 interactions.
DIPiDIP-724N.
IntActiQ08639. 6 interactions.
MINTiMINT-4137330.
STRINGi10090.ENSMUSP00000127952.

Structurei

3D structure databases

ProteinModelPortaliQ08639.
SMRiQ08639. Positions 113-195, 199-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni105 – 12723Interaction with CEBPABy similarityAdd
BLAST
Regioni204 – 27774DimerizationSequence analysisAdd
BLAST
Regioni211 – 327117Enhances binding of RB protein to E2FBy similarityAdd
BLAST
Regioni214 – 24633DCB1Add
BLAST
Regioni259 – 31557DCB2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi161 – 19535DEF boxAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi250 – 2534Poly-Pro
Compositional biasi395 – 41016Asp/Glu-rich (acidic; NCB domain)Add
BLAST

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiKOG2829. Eukaryota.
ENOG410Y9QP. LUCA.
HOGENOMiHOG000030696.
HOVERGENiHBG009894.
InParanoidiQ08639.
KOiK04683.
OMAiHPSTVNT.
OrthoDBiEOG7C2R27.
PhylomeDBiQ08639.
TreeFamiTF314396.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR028313. DP-1.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR014889. Transc_factor_DP_C.
IPR015648. Transcrpt_fac_DP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12548. PTHR12548. 1 hit.
PTHR12548:SF4. PTHR12548:SF4. 1 hit.
PfamiPF08781. DP. 1 hit.
PF02319. E2F_TDP. 1 hit.
[Graphical view]
PIRSFiPIRSF009404. Transcription_factor_DP. 1 hit.
SMARTiSM01138. DP. 1 hit.
SM01372. E2F_TDP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequencei

Sequence statusi: Complete.

Q08639-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKDASLIEA NGELKVFIDQ NLSPGKGVVS LVAVHPSTVN TLGKQLLPKT
60 70 80 90 100
FGQSNVNITQ QVVIGTPQRP AASNTIVVGS PHTPNTHFVS QNQTSDSSPW
110 120 130 140 150
SAGKRNRKGE KNGKGLRHFS MKVCEKVQRK GTTSYNEVAD ELVAEFSAAD
160 170 180 190 200
NHILPNESAY DQKNIRRRVY DALNVLMAMN IISKEKKEIK WIGLPTNSAQ
210 220 230 240 250
ECQNLEVERQ RRLERIKQKQ SQLQELILQQ IAFKNLVQRN RQAEQQARRP
260 270 280 290 300
PPPNSVIHLP FIIVNTSRKT VIDCSISNDK FEYLFNFDNT FEIHDDIEVL
310 320 330 340 350
KRMGMACGLE SGNCSAEDLK VARSLVPKAL EPYVTEMAQG SIGGVFVTTT
360 370 380 390 400
GSTSNGTRLS ASDLSNGADG MLATSSNGSQ YSGSRVETPV SYVGEDDDDD
410
DDFNENDEED
Length:410
Mass (Da):45,231
Last modified:November 1, 1996 - v1
Checksum:iFEEA090C781071B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72310 mRNA. Translation: CAA51056.1.
CCDSiCCDS22110.1.
PIRiB48585.
RefSeqiNP_001278694.1. NM_001291765.1.
NP_033387.1. NM_009361.3.
XP_006508814.1. XM_006508751.2.
XP_006508816.1. XM_006508753.2.
UniGeneiMm.925.

Genome annotation databases

EnsembliENSMUST00000170909; ENSMUSP00000127952; ENSMUSG00000038482.
GeneIDi21781.
KEGGimmu:21781.
UCSCiuc009kxp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72310 mRNA. Translation: CAA51056.1.
CCDSiCCDS22110.1.
PIRiB48585.
RefSeqiNP_001278694.1. NM_001291765.1.
NP_033387.1. NM_009361.3.
XP_006508814.1. XM_006508751.2.
XP_006508816.1. XM_006508753.2.
UniGeneiMm.925.

3D structure databases

ProteinModelPortaliQ08639.
SMRiQ08639. Positions 113-195, 199-346.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204138. 7 interactions.
DIPiDIP-724N.
IntActiQ08639. 6 interactions.
MINTiMINT-4137330.
STRINGi10090.ENSMUSP00000127952.

PTM databases

PhosphoSiteiQ08639.

Proteomic databases

EPDiQ08639.
PaxDbiQ08639.
PRIDEiQ08639.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000170909; ENSMUSP00000127952; ENSMUSG00000038482.
GeneIDi21781.
KEGGimmu:21781.
UCSCiuc009kxp.2. mouse.

Organism-specific databases

CTDi7027.
MGIiMGI:101934. Tfdp1.

Phylogenomic databases

eggNOGiKOG2829. Eukaryota.
ENOG410Y9QP. LUCA.
HOGENOMiHOG000030696.
HOVERGENiHBG009894.
InParanoidiQ08639.
KOiK04683.
OMAiHPSTVNT.
OrthoDBiEOG7C2R27.
PhylomeDBiQ08639.
TreeFamiTF314396.

Enzyme and pathway databases

ReactomeiR-MMU-113510. E2F mediated regulation of DNA replication.
R-MMU-1538133. G0 and Early G1.
R-MMU-2173796. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
R-MMU-69205. G1/S-Specific Transcription.
R-MMU-69231. Cyclin D associated events in G1.

Miscellaneous databases

NextBioi301122.
PROiQ08639.
SOURCEiSearch...

Gene expression databases

BgeeiQ08639.
CleanExiMM_TFDP1.
ExpressionAtlasiQ08639. baseline and differential.
GenevisibleiQ08639. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR028313. DP-1.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR014889. Transc_factor_DP_C.
IPR015648. Transcrpt_fac_DP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12548. PTHR12548. 1 hit.
PTHR12548:SF4. PTHR12548:SF4. 1 hit.
PfamiPF08781. DP. 1 hit.
PF02319. E2F_TDP. 1 hit.
[Graphical view]
PIRSFiPIRSF009404. Transcription_factor_DP. 1 hit.
SMARTiSM01138. DP. 1 hit.
SM01372. E2F_TDP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. Cited for: SEQUENCE REVISION TO 388-410.
  3. "Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation."
    Helin K., Wu C.-L., Fattaey A.R., Lees J.A., Dynlacht B.D., Ngwu C., Harlow E.
    Genes Dev. 7:1850-1861(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Expression patterns of the E2F family of transcription factors during murine epithelial development."
    Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
    Cell Growth Differ. 8:553-563(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  5. "Expression patterns of the E2F family of transcription factors during mouse nervous system development."
    Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
    Mech. Dev. 66:13-25(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung and Spleen.
  7. "Repression of transcriptional activity of C/EBPalpha by E2F-dimerization partner complexes."
    Zaragoza K., Begay V., Schuetz A., Heinemann U., Leutz A.
    Mol. Cell. Biol. 30:2293-2304(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiTFDP1_MOUSE
AccessioniPrimary (citable) accession number: Q08639
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.