Q08637 (NTPB_ENTHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
V-type sodium ATPase subunit B

Alternative name(s):
Na(+)-translocating ATPase subunit B
V-type sodium pump subunit B

Gene names

Name:	ntpB
Ordered Locus Names:	EHR_08265

Organism

Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258) [Complete proteome] [HAMAP]

Taxonomic identifier

768486 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Enterococcaceae › Enterococcus › Enterococcus hirae

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in ATP-driven sodium extrusion. HAMAP-Rule MF_00310
Induction	By increasing intracellular Na+ concentration. HAMAP-Rule MF_00310
Sequence similarities	Belongs to the ATPase alpha/beta chains family.

Ontologies

Keywords
Biological process	Ion transport Sodium transport Transport
Ligand	Sodium
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	ATP hydrolysis coupled proton transport Inferred from electronic annotation. Source: InterPro plasma membrane ATP synthesis coupled proton transport Inferred from electronic annotation. Source: HAMAP sodium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	proton-transporting two-sector ATPase complex, catalytic domain Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP proton-transporting ATP synthase activity, rotational mechanism Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 458

458

V-type sodium ATPase subunit B HAMAP-Rule MF_00310

PRO_0000144685

Secondary structure

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458

Helix Strand Turn

Details...

Beta strand

8 – 12

5

Beta strand

15 – 20

6

Beta strand

29 – 33

5

Beta strand

39 – 56

18

Turn

65 – 67

3

Beta strand

69 – 72

4

Beta strand

78 – 81

4

Helix

83 – 85

3

Beta strand

88 – 91

4

Beta strand

96 – 98

3

Beta strand

106 – 112

7

Turn

118 – 120

3

Beta strand

127 – 129

3

Helix

133 – 136

4

Turn

137 – 139

3

Beta strand

149 – 151

3

Helix

157 – 167

11

Beta strand

169 – 173

5

Beta strand

177 – 186

10

Helix

188 – 201

14

Helix

203 – 206

4

Beta strand

207 – 213

7

Helix

218 – 237

20

Beta strand

242 – 248

7

Helix

250 – 263

14

Helix

270 – 272

3

Helix

277 – 285

9

Beta strand

289 – 291

3

Beta strand

297 – 305

9

Helix

307 – 309

3

Helix

314 – 322

9

Beta strand

323 – 329

7

Helix

331 – 335

5

Turn

344 – 346

3

Helix

352 – 354

3

Turn

358 – 360

3

Helix

365 – 389

25

Helix

391 – 393

3

Helix

396 – 411

16

Helix

423 – 434

12

Turn

439 – 441

3

Helix

447 – 453

7

Sequences

	Sequence		Length	Mass (Da)
	Q08637 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D89D8F2F58B65262	FASTA	458	51,142
	10 20 30 40 50 60 MIKEYRTIKE VVGPLMAVEK VSGVKYEELI EVRMQNGEIR RGQVLEVQED KAMVQIFEGT 70 80 90 100 110 120 SGINLKNSSV RFLGHPLQLG VSEDMIGRVF DGLGRPKDNG PEILPEKYLD INGEVINPIA 130 140 150 160 170 180 RDYPDEFIQT GISAIDHLNT LVRGQKLPVF SGSGLPHKEL AAQIARQATV LDSSDDFAVV 190 200 210 220 230 240 FAAIGITFEE AEFFMEDFRQ TGAIDRSVMF MNLANDPAIE RIATPRMALT AAEYLAYEKG 250 260 270 280 290 300 MHVLVIMTDM TNYAEALREI SAARREVPGR RGYPGYLYTN LATLFERAGR IRGLKGSVTQ 310 320 330 340 350 360 IPILTMPEDD KTHPIPDLTG YITEGQIILT RELYKSGIQP PIDVLPSLSR LKDKGTGAGK 370 380 390 400 410 420 TREDHAATMN QLFAAYAQGK QAKELAVVLG ESALSDIDKI YAKFAERFEN EYVNQGFYTN 430 440 450 RTITETLDLG WELLAMLPRT ELKRIKDDLL DKYLPEGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the genes coding for the A and B subunits of vacuolar-type Na(+)-ATPase from Enterococcus hirae. Coexistence of vacuolar- and F0F1-type ATPases in one bacterial cell." Takase K., Yamato I., Kakinuma Y. J. Biol. Chem. 268:11610-11616(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-25. Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
[2]	"Genome sequence of Enterococcus hirae (Streptococcus faecalis) ATCC 9790, a model organism for the study of ion transport, bioenergetics, and copper homeostasis." Gaechter T., Wunderlin C., Schmidheini T., Solioz M. J. Bacteriol. 194:5126-5127(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D13816 Genomic DNA. Translation: BAA02970.1.
D17462 Genomic DNA. Translation: BAA04276.1.
CP003504 Genomic DNA. Translation: AFM70580.1.

PIR

B46733.

RefSeq

YP_006487471.1. NC_018081.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3VR2	X-ray	2.80	D/E/F	1-458	[»]
3VR3	X-ray	3.40	D/E/F	1-458	[»]
3VR4	X-ray	2.17	D/E/F	1-458	[»]
3VR5	X-ray	3.90	D/E/F	1-458	[»]
3VR6	X-ray	2.68	D/E/F	1-458	[»]

ProteinModelPortal

Q08637.

SMR

Q08637. Positions 13-456.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

13177594.

KEGG

ehr:EHR_08265.

Organism-specific databases

CMR

Search...

Phylogenomic databases

KO

K02118.

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-14147.

Family and domain databases

HAMAP

MF_00310. ATP_synth_B_arch.

InterPro

IPR020003. ATPase_a/bsu_AS.
IPR004100. ATPase_a/bsu_N.
IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR022879. V-ATPase_su_B/beta.
[Graphical view]

Pfam

PF00006. ATP-synt_ab. 1 hit.
PF00306. ATP-synt_ab_C. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
[Graphical view]

PROSITE

PS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

NTPB_ENTHA

Accession

Primary (citable) accession number: Q08637
Secondary accession number(s): I6S1L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program