Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q08636

- NTPA_ENTHA

UniProt

Q08636 - NTPA_ENTHA

Protein

V-type sodium ATPase catalytic subunit A

Gene

ntpA

Organism
Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in ATP-driven sodium extrusion.

    Catalytic activityi

    ATP + H2O + Na+(In) = ADP + phosphate + Na+(Out).

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi232 – 2398ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. proton-transporting ATP synthase activity, rotational mechanism Source: UniProtKB-HAMAP

    GO - Biological processi

    1. ATP hydrolysis coupled proton transport Source: InterPro
    2. plasma membrane ATP synthesis coupled proton transport Source: UniProtKB-HAMAP
    3. sodium ion transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Ion transport, Sodium transport, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, Sodium

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14146.

    Protein family/group databases

    TCDBi3.A.2.2.2. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    V-type sodium ATPase catalytic subunit A (EC:3.6.3.15)
    Alternative name(s):
    Na(+)-translocating ATPase subunit A
    V-type sodium pump catalytic subunit A
    Gene namesi
    Name:ntpA
    Ordered Locus Names:EHR_08260
    OrganismiEnterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258)
    Taxonomic identifieri768486 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
    ProteomesiUP000002895: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. proton-transporting two-sector ATPase complex, catalytic domain Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 593593V-type sodium ATPase catalytic subunit APRO_0000144622Add
    BLAST

    Expressioni

    Inductioni

    By increasing intracellular Na+ concentration.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-60125N.

    Structurei

    Secondary structure

    1
    593
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 107
    Beta strandi13 – 186
    Beta strandi27 – 304
    Turni31 – 344
    Beta strandi35 – 439
    Beta strandi46 – 538
    Beta strandi63 – 664
    Beta strandi68 – 769
    Helixi91 – 988
    Beta strandi100 – 1023
    Beta strandi118 – 1225
    Beta strandi129 – 1313
    Beta strandi135 – 1395
    Beta strandi142 – 1443
    Beta strandi147 – 1504
    Beta strandi157 – 1626
    Beta strandi165 – 1673
    Beta strandi174 – 1785
    Beta strandi181 – 1855
    Beta strandi189 – 1924
    Beta strandi200 – 2034
    Helixi214 – 2196
    Beta strandi227 – 2315
    Helixi238 – 24811
    Beta strandi252 – 26110
    Helixi263 – 27210
    Turni273 – 2753
    Turni279 – 2813
    Beta strandi282 – 2843
    Helixi285 – 2884
    Beta strandi289 – 2957
    Helixi300 – 31819
    Turni319 – 3213
    Beta strandi323 – 3297
    Helixi331 – 34414
    Helixi351 – 3533
    Helixi358 – 3669
    Beta strandi369 – 3735
    Beta strandi381 – 39010
    Beta strandi393 – 3953
    Beta strandi397 – 3993
    Helixi400 – 4067
    Beta strandi410 – 4123
    Helixi417 – 4204
    Turni421 – 4233
    Turni430 – 4323
    Helixi438 – 44912
    Helixi453 – 47624
    Helixi479 – 4813
    Helixi484 – 49916
    Beta strandi505 – 5073
    Turni508 – 5114
    Helixi515 – 53622
    Turni537 – 5393
    Helixi542 – 5487
    Helixi550 – 5578
    Helixi559 – 5613
    Helixi564 – 5674
    Helixi568 – 5714
    Helixi573 – 58614

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3VR2X-ray2.80A/B/C1-593[»]
    3VR3X-ray3.40A/B/C1-593[»]
    3VR4X-ray2.17A/B/C1-593[»]
    3VR5X-ray3.90A/B/C1-593[»]
    3VR6X-ray2.68A/B/C1-593[»]
    ProteinModelPortaliQ08636.
    SMRiQ08636. Positions 59-589.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATPase alpha/beta chains family.Curated

    Phylogenomic databases

    KOiK02117.

    Family and domain databases

    Gene3Di1.10.1140.10. 2 hits.
    3.40.50.300. 1 hit.
    HAMAPiMF_00309. ATP_synth_A_arch.
    InterProiIPR020003. ATPase_a/bsu_AS.
    IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
    IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
    IPR004100. ATPase_F1_a/bsu_N.
    IPR024034. ATPase_F1_bsu/V1_C.
    IPR027417. P-loop_NTPase.
    IPR022878. V-ATPase_asu.
    [Graphical view]
    PfamiPF00006. ATP-synt_ab. 1 hit.
    PF00306. ATP-synt_ab_C. 1 hit.
    PF02874. ATP-synt_ab_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF47917. SSF47917. 1 hit.
    SSF50615. SSF50615. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q08636-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQIGKIIKVS GPLVMAENMS EASIQDMCLV GDLGVIGEII EMRQDVASIQ    50
    VYEETSGIGP GEPVRSTGEA LSVELGPGII SQMFDGIQRP LDTFMEVTQS 100
    NFLGRGVQLP ALDHEKQWWF EATIEEGTEV SAGDIIGYVD ETKIIQHKIM 150
    VPNGIKGTVQ KIESGSFTID DPICVIETEQ GLKELTMMQK WPVRRGRPIK 200
    QKLNPDVPMI TGQRVIDTFF PVTKGGAAAV PGPFGAGKTV VQHQIAKWSD 250
    VDLVVYVGCG ERGNEMTDVV NEFPELIDPN TGESLMERTV LIANTSNMPV 300
    AAREASIYTG ITIAEYFRDM GYDVAIMADS TSRWAEALRE MSGRLEEMPG 350
    DEGYPAYLGS RLAEYYERSG RVIALGSDQR EGSITAISAV SPSGGDISEP 400
    VTQNTLRVVK VFWGLDSSLA QKRHFPSINW IQSYSLYSTE VGRYMDQILQ 450
    QDWSDMVTEG MRILQEEEQL NEIVRLVGID SLSDNDRLTL EVAKSIREDY 500
    LQQNAFDDVD TFTSREKQFN MLKVILTFGK EARKALSLGA YFNEIMEGTV 550
    AVRERISRSK YIPEEELAKI SSINEEIKET IQLIVSEGGM TDD 593
    Length:593
    Mass (Da):65,770
    Last modified:November 1, 1995 - v1
    Checksum:i3E209C2D8687B48A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13816 Genomic DNA. Translation: BAA02969.1.
    D17462 Genomic DNA. Translation: BAA04275.1.
    CP003504 Genomic DNA. Translation: AFM70579.1.
    X76913 Genomic DNA. Translation: CAA54241.1.
    PIRiA46733.
    RefSeqiYP_006487470.1. NC_018081.1.

    Genome annotation databases

    EnsemblBacteriaiAFM70579; AFM70579; EHR_08260.
    GeneIDi13177593.
    KEGGiehr:EHR_08260.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13816 Genomic DNA. Translation: BAA02969.1 .
    D17462 Genomic DNA. Translation: BAA04275.1 .
    CP003504 Genomic DNA. Translation: AFM70579.1 .
    X76913 Genomic DNA. Translation: CAA54241.1 .
    PIRi A46733.
    RefSeqi YP_006487470.1. NC_018081.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3VR2 X-ray 2.80 A/B/C 1-593 [» ]
    3VR3 X-ray 3.40 A/B/C 1-593 [» ]
    3VR4 X-ray 2.17 A/B/C 1-593 [» ]
    3VR5 X-ray 3.90 A/B/C 1-593 [» ]
    3VR6 X-ray 2.68 A/B/C 1-593 [» ]
    ProteinModelPortali Q08636.
    SMRi Q08636. Positions 59-589.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60125N.

    Protein family/group databases

    TCDBi 3.A.2.2.2. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AFM70579 ; AFM70579 ; EHR_08260 .
    GeneIDi 13177593.
    KEGGi ehr:EHR_08260.

    Phylogenomic databases

    KOi K02117.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-14146.

    Family and domain databases

    Gene3Di 1.10.1140.10. 2 hits.
    3.40.50.300. 1 hit.
    HAMAPi MF_00309. ATP_synth_A_arch.
    InterProi IPR020003. ATPase_a/bsu_AS.
    IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
    IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
    IPR004100. ATPase_F1_a/bsu_N.
    IPR024034. ATPase_F1_bsu/V1_C.
    IPR027417. P-loop_NTPase.
    IPR022878. V-ATPase_asu.
    [Graphical view ]
    Pfami PF00006. ATP-synt_ab. 1 hit.
    PF00306. ATP-synt_ab_C. 1 hit.
    PF02874. ATP-synt_ab_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47917. SSF47917. 1 hit.
    SSF50615. SSF50615. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS00152. ATPASE_ALPHA_BETA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of the genes coding for the A and B subunits of vacuolar-type Na(+)-ATPase from Enterococcus hirae. Coexistence of vacuolar- and F0F1-type ATPases in one bacterial cell."
      Takase K., Yamato I., Kakinuma Y.
      J. Biol. Chem. 268:11610-11616(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-20; 345-359 AND 381-407.
      Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
    2. "Genome sequence of Enterococcus hirae (Streptococcus faecalis) ATCC 9790, a model organism for the study of ion transport, bioenergetics, and copper homeostasis."
      Gaechter T., Wunderlin C., Schmidheini T., Solioz M.
      J. Bacteriol. 194:5126-5127(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
    3. "Operon of vacuolar-type Na(+)-ATPase of Enterococcus hirae."
      Solioz M., Davies K.
      J. Biol. Chem. 269:9453-9459(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-187.
      Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.

    Entry informationi

    Entry nameiNTPA_ENTHA
    AccessioniPrimary (citable) accession number: Q08636
    Secondary accession number(s): I6TB93
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3