Q08629 (TICN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Testican-1

Alternative name(s):
Protein SPOCK

Gene names

Name:	SPOCK1
Synonyms:	SPOCK, TIC1, TICN1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in cell-cell and cell-matrix interactions. May contribute to various neuronal mechanisms in the central nervous system.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Post-translational modification	Contains chondroitin sulfate and heparan sulfate O-linked oligosaccharides.
Sequence similarities	Contains 1 Kazal-like domain. Contains 1 thyroglobulin type-1 domain.
Sequence caution	The sequence BAD92938.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Domain	Signal
PTM	Disulfide bond Glycoprotein Heparan sulfate Proteoglycan
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cell adhesion Non-traceable author statement Ref.1. Source: UniProtKB central nervous system neuron differentiation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell-substrate adhesion Inferred from direct assay. Source: UniProtKB negative regulation of neuron projection development Inferred from direct assay. Source: UniProtKB neuron migration Inferred from sequence or structural similarity. Source: UniProtKB regulation of cell growth Non-traceable author statement Ref.1. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from direct assay. Source: UniProtKB neuromuscular junction Inferred from sequence or structural similarity. Source: UniProtKB node of Ranvier Inferred from sequence or structural similarity. Source: UniProtKB postsynaptic density Non-traceable author statement. Source: UniProtKB proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell sarcoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	calcium ion binding Inferred from direct assay. Source: UniProtKB cysteine-type endopeptidase inhibitor activity Inferred from direct assay. Source: UniProtKB metalloendopeptidase inhibitor activity Inferred from direct assay. Source: UniProtKB serine-type endopeptidase inhibitor activity Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 439	418	Testican-1		PRO_0000026699
Regions
	Domain	130 – 182	53	Kazal-like
	Domain	310 – 376	67	Thyroglobulin type-1
Amino acid modifications
	Glycosylation	383	1	O-linked (Xyl...) (glycosaminoglycan) Potential
	Glycosylation	388	1	O-linked (Xyl...) (glycosaminoglycan) Potential
	Disulfide bond	86 ↔ 97		By similarity
	Disulfide bond	91 ↔ 107		By similarity
	Disulfide bond	136 ↔ 166		By similarity
	Disulfide bond	139 ↔ 159		By similarity
	Disulfide bond	148 ↔ 180		By similarity
	Disulfide bond	313 ↔ 337		By similarity
	Disulfide bond	348 ↔ 355		By similarity
	Disulfide bond	357 ↔ 376		By similarity
Experimental info
	Sequence conflict	62	1	D → DEVE in BAD92938. Ref.4
	Sequence conflict	77	1	Q → K in AAH30691. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q08629 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1971601D90F1D0AD	FASTA	439	49,124
	10 20 30 40 50 60 MPAIAVLAAA AAAWCFLQVE SRHLDALAGG AGPNHGNFLD NDQWLSTVSQ YDRDKYWNRF 70 80 90 100 110 120 RDDDYFRNWN PNKPFDQALD PSKDPCLKVK CSPHKVCVTQ DYQTALCVSR KHLLPRQKKG 130 140 150 160 170 180 NVAQKHWVGP SNLVKCKPCP VAQSAMVCGS DGHSYTSKCK LEFHACSTGK SLATLCDGPC 190 200 210 220 230 240 PCLPEPEPPK HKAERSACTD KELRNLASRL KDWFGALHED ANRVIKPTSS NTAQGRFDTS 250 260 270 280 290 300 ILPICKDSLG WMFNKLDMNY DLLLDPSEIN AIYLDKYEPC IKPLFNSCDS FKDGKLSNNE 310 320 330 340 350 360 WCYCFQKPGG LPCQNEMNRI QKLSKGKSLL GAFIPRCNEE GYYKATQCHG STGQCWCVDK 370 380 390 400 410 420 YGNELAGSRK QGAVSCEEEQ ETSGDFGSGG SVVLLDDLEY ERELGPKDKE GKLRVHTRAV 430 TEDDEDEDDD KEDEVGYIW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Testican, a multidomain testicular proteoglycan resembling modulators of cell social behaviour." Alliel P.M., Perin J.-P., Jolles P., Bonnet F.J. Eur. J. Biochem. 214:347-350(1993) [PubMed: 8389704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 344-347 AND 370-393. Tissue: Testis.
[2]	"Endothelial cell expression of testican mRNA." Marr H.S., Basalamah M.A., Edgell C.J. Endothelium 5:209-219(1997) [PubMed: 9272383] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cerebellum.
[4]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed: 15372022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hippocampus.
[7]	"Characterization of a human seminal plasma glycosaminoglycan-bearing polypeptide." Bonnet F., Perin J.-P., Maillet P., Jolles P., Alliel P.M. Biochem. J. 288:565-569(1992) [PubMed: 1463459] [Abstract] Cited for: PROTEIN SEQUENCE OF 370-392, GLYCOSYLATION. Tissue: Seminal plasma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X73608 mRNA. Translation: CAA51999.1. AF231124 mRNA. Translation: AAF43687.1. AK094489 mRNA. Translation: BAG52875.1. AB209701 mRNA. Translation: BAD92938.1. Different initiation. AC005213 Genomic DNA. Translation: AAC24945.1. AC091818 Genomic DNA. No translation available. AC106746 Genomic DNA. No translation available. AC106809 Genomic DNA. No translation available. BC030691 mRNA. Translation: AAH30691.1.
IPI	IPI00005292.
PIR	S33293.
RefSeq	NP_004589.1. NM_004598.3.
UniGene	Hs.596136.
3D structure databases
ProteinModelPortal	Q08629.
SMR	Q08629. Positions 80-309, 311-378.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q08629. 1 interaction.
STRING	Q08629.
Protein family/group databases
MEROPS	I31.006.
PTM databases
PhosphoSite	Q08629.
Polymorphism databases
DMDM	24212472.
Proteomic databases
PRIDE	Q08629.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000282223; ENSP00000282223; ENSG00000152377. ENST00000394945; ENSP00000378401; ENSG00000152377.
GeneID	6695.
KEGG	hsa:6695.
UCSC	uc003lbo.1. human.
Organism-specific databases
CTD	6695.
GeneCards	GC05M136339.
H-InvDB	HIX0005200.
HGNC	HGNC:11251. SPOCK1.
HPA	HPA007450.
MIM	602264. gene.
neXtProt	NX_Q08629.
PharmGKB	PA36081.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG12736.
GeneTree	ENSGT00510000046429.
HOGENOM	HBG444198.
HOVERGEN	HBG026595.
InParanoid	Q08629.
OMA	RQKKGNM.
OrthoDB	EOG4MSCZ5.
PhylomeDB	Q08629.
Gene expression databases
ArrayExpress	Q08629.
Bgee	Q08629.
CleanEx	HS_SPOCK1.
Genevestigator	Q08629.
Family and domain databases
InterPro	IPR011992. EF-hand-like_dom. IPR002350. Prot_inh_Kazal. IPR011497. Prot_Inh_Kazal_2. IPR019577. SPARC/Testican_Ca-bd-dom. IPR000716. Thyroglobulin_1. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit. G3DSA:4.10.800.10. Thyroglobulin_1. 1 hit.
KO	K08136.
Pfam	PF07648. Kazal_2. 1 hit. PF10591. SPARC_Ca_bdg. 1 hit. PF00086. Thyroglobulin_1. 1 hit. [Graphical view]
SMART	SM00280. KAZAL. 1 hit. SM00211. TY. 1 hit. [Graphical view]
SUPFAM	SSF57610. Thyroglobulin_1. 1 hit.
PROSITE	PS51465. KAZAL_2. 1 hit. PS00484. THYROGLOBULIN_1_1. 1 hit. PS51162. THYROGLOBULIN_1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	26099.
SOURCE	Search...

Entry information

Entry name

TICN1_HUMAN

Accession

Primary (citable) accession number: Q08629
Secondary accession number(s): B3KSW3 , Q59EW0, Q8N630, Q9UCL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.