Reviewed, UniProtKB/Swiss-Prot Q08603 (PGTB2_RAT)

Names and origin

Protein names

Recommended name:
    Geranylgeranyl transferase type-2 subunit beta
    EC=2.5.1.60
Alternative name(s):
    Geranylgeranyl transferase type II subunit beta
      Short name=GGTase-II-beta
    Type II protein geranyl-geranyltransferase subunit beta
    Rab geranylgeranyltransferase subunit beta
    Rab geranyl-geranyltransferase subunit beta
      Short name=Rab GG transferase beta
      Short name=Rab GGTase beta

Gene names

Name:	Rabggtb
Synonyms:	Ggtb

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to both cysteines in Rab proteins with an -XXCC, -XCXC and -CCXX C-terminal, such as RAB1A, RAB3A and RAB5A respectively.
Catalytic activity	Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.
Cofactor	Binds 1 zinc ion per dimer.
Enzyme regulation	The enzymatic reaction requires the aid of a Rab escort protein (also called component A).
Subunit structure	Heterodimer of an alpha and a beta subunit, collectively called component B. Interaction of beta subunit with prenylated PTP4A2 precludes its association with subunit alpha, and inhibits enzyme activity By similarity.
Tissue specificity	Most abundant in the heart, brain, spleen and liver. Less in the lung, muscle, kidney and testis; in these tissues, more abundant than the subunit alpha.
Sequence similarities	Belongs to the protein prenyltransferase subunit beta family. Contains 6 PFTB repeats.

Ontologies

Keywords
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Prenyltransferase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	protein amino acid geranylgeranylation Ref.1 Inferred from direct assay. Source: RGD
Molecular function	Rab geranylgeranyltransferase activity Ref.1 Inferred from direct assay. Source: RGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 331

331

Geranylgeranyl transferase type-2 subunit beta

PRO_0000119774

Regions

Repeat

20 – 61

42

PFTB 1

Repeat

68 – 109

42

PFTB 2

Repeat

116 – 157

42

PFTB 3

Repeat

164 – 205

42

PFTB 4

Repeat

212 – 253

42

PFTB 5

Repeat

260 – 302

43

PFTB 6

Sites

Metal binding

238

1

Zinc

Metal binding

240

1

Zinc

Metal binding

290

1

Zinc

Amino acid modifications

Modified residue

3

1

Phosphothreonine By similarity

Secondary structure

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331

Helix Strand Turn

Details...

Helix

20 – 29

10

Helix

43 – 45

3

Helix

46 – 59

14

Helix

63 – 65

3

Helix

68 – 78

11

Beta strand

85 – 88

4

Helix

95 – 107

13

Helix

116 – 124

9

Beta strand

133 – 136

4

Helix

143 – 154

12

Turn

155 – 157

3

Helix

164 – 173

10

Helix

191 – 203

13

Helix

207 – 209

3

Helix

212 – 220

9

Beta strand

231 – 234

4

Helix

239 – 251

13

Helix

255 – 257

3

Helix

260 – 269

10

Turn

273 – 275

3

Beta strand

277 – 281

5

Helix

288 – 301

14

Turn

311 – 313

3

Beta strand

314 – 316

3

Helix

317 – 322

6

Sequences

	Sequence		Length	Mass (Da)
	Q08603-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 4B12DEC0245979D6	FASTA	331	36,856
	10 20 30 40 50 60 MGTQQKDVTI KSDAPDTLLL EKHADYIASY GSKKDDYEYC MSEYLRMSGV YWGLTVMDLM 70 80 90 100 110 120 GQLHRMNKEE ILVFIKSCQH ECGGVSASIG HDPHLLYTLS AVQILTLYDS IHVINVDKVV 130 140 150 160 170 180 AYVQSLQKED GSFAGDIWGE IDTRFSFCAV ATLALLGKLD AINVEKAIEF VLSCMNFDGG 190 200 210 220 230 240 FGCRPGSESH AGQIYCCTGF LAITSQLHQV NSDLLGWWLC ERQLPSGGLN GRPEKLPDVC 250 260 270 280 290 300 YSWWVLASLK IIGRLHWIDR EKLRSFILAC QDEETGGFAD RPGDMVDPFH TLFGIAGLSL 310 320 330 LGEEQIKPVS PVFCMPEEVL QRVNVQPELV S

References

[1]	"cDNA cloning and expression of the alpha and beta subunits of rat Rab geranylgeranyl transferase." Armstrong S.A., Seabra M.C., Suedhof T.C., Goldstein J.L., Brown M.S. J. Biol. Chem. 268:12221-12229(1993) [PubMed: 8505342] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Brain.
[2]	"Crystal structure of Rab geranylgeranyltransferase at 2.0 A resolution." Zhang H., Seabra M.C., Deisenhofer J. Structure 8:241-251(2000) [PubMed: 10745007] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH ALPHA SUBUNIT.
[3]	"Structure of Rab escort protein-1 in complex with Rab geranylgeranyltransferase." Pylypenko O., Rak A., Reents R., Niculae A., Sidorovitch V., Cioaca M.D., Bessolitsyna E., Thomae N.H., Waldmann H., Schlichting I., Goody R.S., Alexandrov K. Mol. Cell 11:483-494(2003) [PubMed: 12620235] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH ALPHA SUBUNIT AND REP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L10416 mRNA. Translation: AAA41999.1.
S62097 mRNA. Translation: AAB27019.1.

IPI

IPI00421632.

PIR

B45977.

RefSeq

NP_619715.1.

UniGene

Rn.107818

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DCE	X-ray	2.00	B/D	1-331	[»]
1LTX	X-ray	2.70	B	1-331	[»]
3C72	X-ray	2.30	B	1-331	[»]
3DSS	X-ray	1.80	B	1-331	[»]
3DST	X-ray	1.90	B	1-331	[»]
3DSU	X-ray	1.90	B	1-331	[»]
3DSV	X-ray	2.10	B	1-331	[»]
3DSW	X-ray	2.15	B	1-331	[»]
3DSX	X-ray	2.10	B	1-331	[»]
3HXB	X-ray	2.25	B	1-331	[»]
3HXC	X-ray	1.95	B	1-331	[»]
3HXD	X-ray	1.95	B	1-331	[»]
3HXE	X-ray	1.95	B	1-331	[»]
3HXF	X-ray	1.90	B	1-331	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:6138N.

STRING

Q08603.

Genome annotation databases

Ensembl

ENSRNOT00000013660; ENSRNOP00000013660; ENSRNOG00000009992; Rattus norvegicus. [Genome view]

GeneID

25533.

KEGG

rno:25533.

Organism-specific databases

CTD

25533.

RGD

3530. Rabggtb.

Phylogenomic databases

HOVERGEN

Q08603.

Enzyme and pathway databases

BRENDA

2.5.1.60. 248.

Gene expression databases

ArrayExpress

Q08603.

Genevestigator

Q08603.

GermOnline

ENSRNOG00000009992. Rattus norvegicus.

Family and domain databases

InterPro

IPR001330. Prenyltrans.
[Graphical view]

Pfam

PF00432. Prenyltrans. 5 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

607037.

Entry information

Entry name

PGTB2_RAT

Accession

Primary (citable) accession number: Q08603

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 3, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)