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Protein

Metacaspase-1

Gene

MCA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates cell death (apoptosis) triggered by oxygen stress, salt stress or chronological aging. Regulated cell death can prevent a release of toxic cellular components, thus avoiding necrotic collapse of the colony, and can also provide nutrients for healthy cells. Therefore, regulated cell death in yeast colonies can be as important for their development as are apoptosis and related processes that occur within metazoa. Promotes the removal of insoluble protein aggregates during normal growth.11 Publications

Miscellaneous

Present with 1400 molecules/cell in log phase SD medium.1 Publication

Caution

PubMed:19174511 reported that MCA1 may have a prion form, dubbed [MCA]. However, the same authors have later not been able to reproduce these results and retracted the paper.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei220By similarity1
Active sitei2761

GO - Molecular functioni

  • calcium-dependent cysteine-type endopeptidase activity Source: SGD

GO - Biological processi

  • apoptotic process Source: SGD
  • protein quality control for misfolded or incompletely synthesized proteins Source: SGD

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processApoptosis

Enzyme and pathway databases

BioCyciYEAST:G3O-33705-MONOMER

Protein family/group databases

MEROPSiC14.035

Names & Taxonomyi

Protein namesi
Recommended name:
Metacaspase-1 (EC:3.4.22.-)
Gene namesi
Name:MCA1
Synonyms:YCA1
Ordered Locus Names:YOR197W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR197W
SGDiS000005723 MCA1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi276C → A: Blocks the processing and reduces caspase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000268684? – 432Metacaspase-1
PropeptideiPRO_00002686831 – ?Sequence analysis

Keywords - PTMi

Zymogen

Proteomic databases

MaxQBiQ08601
PaxDbiQ08601
PRIDEiQ08601

Interactioni

Protein-protein interaction databases

BioGridi34595, 130 interactors
DIPiDIP-2802N
IntActiQ08601, 27 interactors
MINTiQ08601
STRINGi4932.YOR197W

Structurei

Secondary structure

1432
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni92 – 94Combined sources3
Beta strandi102 – 104Combined sources3
Beta strandi136 – 142Combined sources7
Helixi155 – 167Combined sources13
Helixi174 – 176Combined sources3
Beta strandi177 – 181Combined sources5
Helixi187 – 189Combined sources3
Helixi193 – 204Combined sources12
Beta strandi212 – 219Combined sources8
Beta strandi221 – 223Combined sources3
Beta strandi237 – 239Combined sources3
Helixi244 – 247Combined sources4
Helixi252 – 259Combined sources8
Turni260 – 262Combined sources3
Beta strandi268 – 273Combined sources6
Beta strandi275 – 277Combined sources3
Turni279 – 282Combined sources4
Beta strandi285 – 289Combined sources5
Beta strandi292 – 295Combined sources4
Beta strandi354 – 362Combined sources9
Helixi380 – 391Combined sources12
Helixi397 – 408Combined sources12
Turni409 – 411Combined sources3
Beta strandi415 – 422Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4F6OX-ray1.68A83-432[»]
4F6PX-ray1.62A83-432[»]
ProteinModelPortaliQ08601
SMRiQ08601
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni29 – 131Prion domain (PrD)Add BLAST103

Domaini

The prion domain (PrD) is a Gln/Asn (Q/N)-rich domain. It targets the protein to insoluble protein aggregates.1 Publication

Sequence similaritiesi

Belongs to the peptidase C14B family.Curated

Phylogenomic databases

HOGENOMiHOG000240109
InParanoidiQ08601
OMAiFFQYSQC
OrthoDBiEOG092C4ES8

Family and domain databases

InterProiView protein in InterPro
IPR029030 Caspase-like_dom_sf
SUPFAMiSSF52129 SSF52129, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08601-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYPGSGRYTY NNAGGNNGYQ RPMAPPPNQQ YGQQYGQQYE QQYGQQYGQQ
60 70 80 90 100
NDQQFSQQYA PPPGPPPMAY NRPVYPPPQF QQEQAKAQLS NGYNNPNVNA
110 120 130 140 150
SNMYGPPQNM SLPPPQTQTI QGTDQPYQYS QCTGRRKALI IGINYIGSKN
160 170 180 190 200
QLRGCINDAH NIFNFLTNGY GYSSDDIVIL TDDQNDLVRV PTRANMIRAM
210 220 230 240 250
QWLVKDAQPN DSLFLHYSGH GGQTEDLDGD EEDGMDDVIY PVDFETQGPI
260 270 280 290 300
IDDEMHDIMV KPLQQGVRLT ALFDSCHSGT VLDLPYTYST KGIIKEPNIW
310 320 330 340 350
KDVGQDGLQA AISYATGNRA ALIGSLGSIF KTVKGGMGNN VDRERVRQIK
360 370 380 390 400
FSAADVVMLS GSKDNQTSAD AVEDGQNTGA MSHAFIKVMT LQPQQSYLSL
410 420 430
LQNMRKELAG KYSQKPQLSS SHPIDVNLQF IM
Length:432
Mass (Da):47,982
Last modified:May 20, 2008 - v2
Checksum:i547D186E3EB481B7
GO

Sequence cautioni

The sequence AAT92851 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA99410 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75105 Genomic DNA Translation: CAA99410.1 Different initiation.
AY692832 Genomic DNA Translation: AAT92851.1 Different initiation.
BK006948 Genomic DNA Translation: DAA10972.1
PIRiS67089
RefSeqiNP_014840.4, NM_001183616.3

Genome annotation databases

EnsemblFungiiYOR197W; YOR197W; YOR197W
GeneIDi854372
KEGGisce:YOR197W

Similar proteinsi

Entry informationi

Entry nameiMCA1_YEAST
AccessioniPrimary (citable) accession number: Q08601
Secondary accession number(s): D6W2Q6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: May 20, 2008
Last modified: June 20, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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