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Protein

Kinetochore protein SLK19

Gene

SLK19

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in spindle assembly and stability. Required to ensure a timely exit form mitosis. Essential to maintain pre-anaphase spindle polarity. Associates to the plus ends of the microtubules at the kinetochore and spindle midzone. A component of the FEAR (CDC14 Early Anaphase Release) network which promotes CDC14 release from the nucleolus during early anaphase. Required for proper chromosome segregation during meiosis I where it prevents premature sister chromatid separation.7 Publications

Miscellaneous

Present with 314 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • centromere clustering Source: SGD
  • chromosome segregation Source: SGD
  • meiotic cell cycle Source: SGD
  • mitotic spindle organization in nucleus Source: SGD
  • nuclear migration Source: SGD
  • positive regulation of exit from mitosis Source: SGD

Keywordsi

Biological processCell cycle, Cell division, Chromosome partition, Meiosis, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-33704-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein SLK19
Alternative name(s):
Synthetic lethal KAR3 protein 19
Gene namesi
Name:SLK19
Ordered Locus Names:YOR195W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR195W.
SGDiS000005721. SLK19.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi77R → E: No cleavage by ESP1. 1 Publication1
Mutagenesisi78S → R: Destabilizes; not detectable in anaphase cells; spindle becomes thin and fragile. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002705731 – 821Kinetochore protein SLK19Add BLAST821

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7Phosphothreonine; by CDC28Curated1
Modified residuei188PhosphoserineCombined sources1
Modified residuei189PhosphoserineCombined sources1
Modified residuei201Phosphoserine; by CDC28Combined sources1
Modified residuei216PhosphoserineCombined sources1
Modified residuei273PhosphothreonineCombined sources1
Modified residuei283PhosphoserineCombined sources1

Post-translational modificationi

Cleaved by ESP1 at the onset of anaphase.
Phosphorylated by CDC5/Polo-like kinase at the onset of anaphase. Phosphorylation takes places at proximity to cleavage sites and is required for an efficient cleavage by ESP1. Phosphorylated also by CDC28.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei77 – 78Cleavage; by ESP12

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08581.
PRIDEiQ08581.

PTM databases

iPTMnetiQ08581.

Miscellaneous databases

PMAP-CutDBiQ08581.

Interactioni

Protein-protein interaction databases

BioGridi34593. 179 interactors.
DIPiDIP-2595N.
IntActiQ08581. 5 interactors.
MINTiMINT-435601.
STRINGi4932.YOR195W.

Structurei

3D structure databases

ProteinModelPortaliQ08581.
SMRiQ08581.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili310 – 821Sequence analysisAdd BLAST512

Keywords - Domaini

Coiled coil

Phylogenomic databases

InParanoidiQ08581.
KOiK12575.
OMAiRECRISA.
OrthoDBiEOG092C4CIO.

Family and domain databases

InterProiView protein in InterPro
IPR024312. TACC_fungi.
PfamiView protein in Pfam
PF12709. Kinetocho_Slk19. 1 hit.

Sequencei

Sequence statusi: Complete.

Q08581-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEVPTTPVR LILGQAQQRE QNSENCSQER NPRTFNSEPD SSFNSPGSSQ
60 70 80 90 100
FVIHPHEPLE KEKDEKQDLD RSIDYGRSSA LNNKNNANPL ENIDINKMFD
110 120 130 140 150
DKKSDSGTND DKGGASTSDK HVLALNYSPI RVEMNSSEKR SDKNVDVDEN
160 170 180 190 200
DKEGSHINKK LKLQLESVPD LKQSSTKDII NDKEEIMSSP MAIDMIETNI
210 220 230 240 250
SPNKFIINDG VERNDSFNIN TDTLKLENDI NEKQQEEDFI KSNSNNVVNI
260 270 280 290 300
DNAYKEKEDE ENDITNSHIN RLTPLYETSA RESNSNEEGR NDYDDDNQLD
310 320 330 340 350
IRHDNFQIVA KRNEELTDQI YHLNQMLNSL ISKNESLSFQ YEKLNKNHQL
360 370 380 390 400
LIDLTNEKLD KLNTERESDI AKVEKFKKRI KELNTEIKVL NSNQKILQEK
410 420 430 440 450
FDASITEVNH IKGEHENTVN TLQQNEKILN DKNVELENMK AELKGNNDKL
460 470 480 490 500
SEYETTLNDL NSRIVQLNDK IESTDIVLKS KENELDNLKL SLKETLSISK
510 520 530 540 550
DFNDSDLIGQ INELISTKNN LQQKMDDLNN LNDDNLKVVQ DKLIKNEETL
560 570 580 590 600
KLKEAEIDSL NSEMDELKKQ ITSKDDEFKM WQSKYETVED EAKIRNAEVT
610 620 630 640 650
ELNGDIEDLK ESKLHLEETI TELENKVHKL ENECELEKQK FEKTSLELES
660 670 680 690 700
LQLKNSNIQA EHIKELENLH ENLISLQNEL KISSDRITTL TKENEVLMEQ
710 720 730 740 750
NNNNNNSVTL SNDQKDRDDE KIKSLGKQVQ DWKEKYEAKE KDTNKRLKLL
760 770 780 790 800
AEDLYIQYSS KHEQKVKLLK KGYENKYQNK FDQLNLENKT LSEEIEQLNK
810 820
QLSSEREEKQ ELLKLLENEK K
Length:821
Mass (Da):95,380
Last modified:November 1, 1996 - v1
Checksum:i994CCE2EFB7C4B67
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75103 Genomic DNA. Translation: CAA99408.1.
BK006948 Genomic DNA. Translation: DAA10970.1.
PIRiS67087.
RefSeqiNP_014838.3. NM_001183614.3.

Genome annotation databases

EnsemblFungiiYOR195W; YOR195W; YOR195W.
GeneIDi854370.
KEGGisce:YOR195W.

Similar proteinsi

Entry informationi

Entry nameiSLK19_YEAST
AccessioniPrimary (citable) accession number: Q08581
Secondary accession number(s): D6W2Q4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: November 22, 2017
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names