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Protein

Kinetochore protein SLK19

Gene

SLK19

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in spindle assembly and stability. Required to ensure a timely exit form mitosis. Essential to maintain pre-anaphase spindle polarity. Associates to the plus ends of the microtubules at the kinetochore and spindle midzone. A component of the FEAR (CDC14 Early Anaphase Release) network which promotes CDC14 release from the nucleolus during early anaphase. Required for proper chromosome segregation during meiosis I where it prevents premature sister chromatid seperation.7 Publications

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • centromere clustering Source: SGD
  • chromosome segregation Source: SGD
  • meiotic nuclear division Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
  • mitotic spindle organization in nucleus Source: SGD
  • nuclear migration Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Meiosis, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-33704-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein SLK19
Alternative name(s):
Synthetic lethal KAR3 protein 19
Gene namesi
Name:SLK19
Ordered Locus Names:YOR195W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR195W.
SGDiS000005721. SLK19.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome kinetochore Source: SGD
  • cytoplasm Source: UniProtKB-KW
  • kinetochore Source: SGD
  • microtubule Source: UniProtKB-KW
  • spindle Source: SGD
  • spindle midzone Source: SGD
  • spindle pole body Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi77R → E: No cleavage by ESP1. 1 Publication1
Mutagenesisi78S → R: Destabilizes; not detectable in anaphase cells; spindle becomes thin and fragile. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002705731 – 821Kinetochore protein SLK19Add BLAST821

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7Phosphothreonine; by CDC28Curated1
Modified residuei188PhosphoserineCombined sources1
Modified residuei189PhosphoserineCombined sources1
Modified residuei201Phosphoserine; by CDC28Combined sources1
Modified residuei216PhosphoserineCombined sources1
Modified residuei273PhosphothreonineCombined sources1
Modified residuei283PhosphoserineCombined sources1

Post-translational modificationi

Cleaved by ESP1 at the onset of anaphase.
Phosphorylated by CDC5/Polo-like kinase at the onset of anaphase. Phosphorylation takes places at proximity to cleavage sites and is required for an efficient cleavage by ESP1. Phosphorylated also by CDC28.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei77 – 78Cleavage; by ESP12

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08581.
PRIDEiQ08581.

PTM databases

iPTMnetiQ08581.

Miscellaneous databases

PMAP-CutDBQ08581.

Interactioni

Protein-protein interaction databases

BioGridi34593. 114 interactors.
DIPiDIP-2595N.
IntActiQ08581. 5 interactors.
MINTiMINT-435601.

Structurei

3D structure databases

ProteinModelPortaliQ08581.
SMRiQ08581.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili310 – 821Sequence analysisAdd BLAST512

Keywords - Domaini

Coiled coil

Phylogenomic databases

InParanoidiQ08581.
KOiK12575.
OMAiAHESEFA.
OrthoDBiEOG092C4CIO.

Family and domain databases

InterProiIPR024312. Kinetocho_Slk19.
[Graphical view]
PfamiPF12709. Kinetocho_Slk19. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08581-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEVPTTPVR LILGQAQQRE QNSENCSQER NPRTFNSEPD SSFNSPGSSQ
60 70 80 90 100
FVIHPHEPLE KEKDEKQDLD RSIDYGRSSA LNNKNNANPL ENIDINKMFD
110 120 130 140 150
DKKSDSGTND DKGGASTSDK HVLALNYSPI RVEMNSSEKR SDKNVDVDEN
160 170 180 190 200
DKEGSHINKK LKLQLESVPD LKQSSTKDII NDKEEIMSSP MAIDMIETNI
210 220 230 240 250
SPNKFIINDG VERNDSFNIN TDTLKLENDI NEKQQEEDFI KSNSNNVVNI
260 270 280 290 300
DNAYKEKEDE ENDITNSHIN RLTPLYETSA RESNSNEEGR NDYDDDNQLD
310 320 330 340 350
IRHDNFQIVA KRNEELTDQI YHLNQMLNSL ISKNESLSFQ YEKLNKNHQL
360 370 380 390 400
LIDLTNEKLD KLNTERESDI AKVEKFKKRI KELNTEIKVL NSNQKILQEK
410 420 430 440 450
FDASITEVNH IKGEHENTVN TLQQNEKILN DKNVELENMK AELKGNNDKL
460 470 480 490 500
SEYETTLNDL NSRIVQLNDK IESTDIVLKS KENELDNLKL SLKETLSISK
510 520 530 540 550
DFNDSDLIGQ INELISTKNN LQQKMDDLNN LNDDNLKVVQ DKLIKNEETL
560 570 580 590 600
KLKEAEIDSL NSEMDELKKQ ITSKDDEFKM WQSKYETVED EAKIRNAEVT
610 620 630 640 650
ELNGDIEDLK ESKLHLEETI TELENKVHKL ENECELEKQK FEKTSLELES
660 670 680 690 700
LQLKNSNIQA EHIKELENLH ENLISLQNEL KISSDRITTL TKENEVLMEQ
710 720 730 740 750
NNNNNNSVTL SNDQKDRDDE KIKSLGKQVQ DWKEKYEAKE KDTNKRLKLL
760 770 780 790 800
AEDLYIQYSS KHEQKVKLLK KGYENKYQNK FDQLNLENKT LSEEIEQLNK
810 820
QLSSEREEKQ ELLKLLENEK K
Length:821
Mass (Da):95,380
Last modified:November 1, 1996 - v1
Checksum:i994CCE2EFB7C4B67
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75103 Genomic DNA. Translation: CAA99408.1.
BK006948 Genomic DNA. Translation: DAA10970.1.
PIRiS67087.
RefSeqiNP_014838.3. NM_001183614.3.

Genome annotation databases

EnsemblFungiiYOR195W; YOR195W; YOR195W.
GeneIDi854370.
KEGGisce:YOR195W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75103 Genomic DNA. Translation: CAA99408.1.
BK006948 Genomic DNA. Translation: DAA10970.1.
PIRiS67087.
RefSeqiNP_014838.3. NM_001183614.3.

3D structure databases

ProteinModelPortaliQ08581.
SMRiQ08581.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34593. 114 interactors.
DIPiDIP-2595N.
IntActiQ08581. 5 interactors.
MINTiMINT-435601.

PTM databases

iPTMnetiQ08581.

Proteomic databases

MaxQBiQ08581.
PRIDEiQ08581.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR195W; YOR195W; YOR195W.
GeneIDi854370.
KEGGisce:YOR195W.

Organism-specific databases

EuPathDBiFungiDB:YOR195W.
SGDiS000005721. SLK19.

Phylogenomic databases

InParanoidiQ08581.
KOiK12575.
OMAiAHESEFA.
OrthoDBiEOG092C4CIO.

Enzyme and pathway databases

BioCyciYEAST:G3O-33704-MONOMER.

Miscellaneous databases

PMAP-CutDBQ08581.
PROiQ08581.

Family and domain databases

InterProiIPR024312. Kinetocho_Slk19.
[Graphical view]
PfamiPF12709. Kinetocho_Slk19. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSLK19_YEAST
AccessioniPrimary (citable) accession number: Q08581
Secondary accession number(s): D6W2Q4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 314 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.