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Q08579 (THI72_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thiamine transporter THI72
Gene names
Name:THI72
Ordered Locus Names:YOR192C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length599 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Low affinity thiamine transporter responsible for intake of thiamine. It is possible that the primary function is the uptake of closely related compounds and that thiamine transport is a secondary activity of these proteins. Ref.3

Subcellular location

Membrane; Multi-pass membrane protein.

Induction

Induced by limited extracellular thiamine levels. Ref.4

Sequence similarities

Belongs to the purine-cytosine permease family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 599599Thiamine transporter THI72
PRO_0000197928

Regions

Transmembrane42 – 6221Helical; Potential
Transmembrane78 – 9821Helical; Potential
Transmembrane112 – 13221Helical; Potential
Transmembrane174 – 19421Helical; Potential
Transmembrane197 – 21721Helical; Potential
Transmembrane280 – 30021Helical; Potential
Transmembrane333 – 35321Helical; Potential
Transmembrane372 – 39221Helical; Potential
Transmembrane395 – 41521Helical; Potential
Transmembrane447 – 46721Helical; Potential
Transmembrane484 – 50421Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
Q08579 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 43872C34C352A2AA

FASTA59967,186
        10         20         30         40         50         60 
MSFGTRISRA LRFLEIPVKN RASVNFLRNP DLQPIKSVNQ TWGFWSNFAY WGVLSFNVGM 

        70         80         90        100        110        120 
WIGGSSALTV GLSYSETIGA FIIADLLTIL FALANSCPGY DWKVGFTLAQ RFVFGIYGSA 

       130        140        150        160        170        180 
LGIIIRILMS IVYYGSNAWL GGLCVNMILD SWSHHYLHLP NTLSSKVAMT TKELIGFIIF 

       190        200        210        220        230        240 
HILTAFCYFM KPYHMNYILI WSCVGTFFAM LGMVIYLTKS AHGVGDLFTS THSTVTGSKK 

       250        260        270        280        290        300 
AWAWVYTISY WYGSVSPGCT NQSDFSRFGS SNCAIWTGTI VALLIPATLI PVFGIIGASA 

       310        320        330        340        350        360 
CEKLYGQTFW MPMDIFDNWL TTNYSAGARA ATFFCGFCFV MSQISYTISN CGFASGMDLA 

       370        380        390        400        410        420 
GLLPKYVDIK RGAIFAACVS WACLPWNFYN SSSTFLTVMS SFGVVMTPII TVMICDNFLI 

       430        440        450        460        470        480 
RKRQYSVTNA FVLKGEYYFT KGVNWRAIVA WVCGMAPGLP GIAWEVNNDY FHNTGIINFF 

       490        500        510        520        530        540 
YGDSFFSFLI SFFVYWGLCL LFPFKITVKH DDKDYYGAFT DEEARKKGMV PYSEISEEEI 

       550        560        570        580        590 
RAYTLGECFT SGHEYKPESS DDELPELTKT SSENTKVFEI VHQKDNEKES STSSEKQIA 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Isolation and characterization of a thiamin transport gene, THI10, from Saccharomyces cerevisiae."
Enjo F., Nosaka K., Ogata M., Iwashima A., Nishimura H.
J. Biol. Chem. 272:19165-19170(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Genetic regulation mediated by thiamin pyrophosphate-binding motif in Saccharomyces cerevisiae."
Nosaka K., Onozuka M., Konno H., Kawasaki Y., Nishimura H., Sano M., Akaji K.
Mol. Microbiol. 58:467-479(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z75100 Genomic DNA. Translation: CAA99401.1.
BK006948 Genomic DNA. Translation: DAA10964.1.
PIRS67084.
RefSeqNP_014835.3. NM_001183611.3.

3D structure databases

ProteinModelPortalQ08579.
SMRQ08579. Positions 27-445.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34587. 34 interactions.
DIPDIP-7926N.
IntActQ08579. 2 interactions.
MINTMINT-2493622.
STRING4932.YOR192C.

Proteomic databases

PaxDbQ08579.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR192C; YOR192C; YOR192C.
GeneID854364.
KEGGsce:YOR192C.

Organism-specific databases

CYGDYOR192c.
SGDS000005718. THI72.

Phylogenomic databases

eggNOGCOG1953.
GeneTreeENSGT00530000065049.
HOGENOMHOG000066000.
OMAICCGLSE.
OrthoDBEOG7C2R9F.

Enzyme and pathway databases

BioCycYEAST:G3O-33701-MONOMER.

Gene expression databases

GenevestigatorQ08579.

Family and domain databases

InterProIPR001248. Cyt_pur_permease.
IPR012681. NCS1.
[Graphical view]
PfamPF02133. Transp_cyt_pur. 1 hit.
[Graphical view]
TIGRFAMsTIGR00800. ncs1. 1 hit.
ProtoNetSearch...

Other

NextBio976479.

Entry information

Entry nameTHI72_YEAST
AccessionPrimary (citable) accession number: Q08579
Secondary accession number(s): D6W2P8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families