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Q08558

- DCI1_YEAST

UniProt

Q08558 - DCI1_YEAST

Protein

Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase

Gene

DCI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Converts 3,5-dienoyl-CoAs to the corresponding 2,4-dienoyl-CoAs. Involved in fatty acid metabolism. Required for ECI1 to be peroxisomal located.3 Publications

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei120 – 1201Substrate; via amide nitrogenBy similarity
    Sitei152 – 1521Important for catalytic activityBy similarity

    GO - Molecular functioni

    1. dodecenoyl-CoA delta-isomerase activity Source: SGD

    GO - Biological processi

    1. fatty acid beta-oxidation Source: SGD

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:YOR180C-MONOMER.
    YEAST:YOR180C-MONOMER.
    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase (EC:5.3.3.-)
    Gene namesi
    Name:DCI1
    Synonyms:ECI2, EHD2
    Ordered Locus Names:YOR180C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR180c.
    SGDiS000005706. DCI1.

    Subcellular locationi

    Peroxisome 3 Publications

    GO - Cellular componenti

    1. peroxisomal matrix Source: SGD

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 271271Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerasePRO_0000232996Add
    BLAST

    Proteomic databases

    PaxDbiQ08558.
    PeptideAtlasiQ08558.

    Expressioni

    Inductioni

    By oleate, through the binding of the OAF1-PIP2 transcriptional activator complex to its promoter ORE element. Expression is also induced during ammonia pulses in yeast colonies, at the beginning of detectable ammonia production.4 Publications

    Gene expression databases

    GenevestigatoriQ08558.

    Interactioni

    Subunit structurei

    Interacts with ECI1.1 Publication

    Protein-protein interaction databases

    BioGridi34575. 21 interactions.
    DIPiDIP-4132N.
    IntActiQ08558. 2 interactions.
    MINTiMINT-386432.
    STRINGi4932.YOR180C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08558.
    SMRiQ08558. Positions 2-262.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni62 – 665Substrate bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi269 – 2713Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1024.
    GeneTreeiENSGT00670000097595.
    HOGENOMiHOG000027948.
    OMAiGQKKHKL.
    OrthoDBiEOG70PC7K.

    Family and domain databases

    Gene3Di3.90.226.10. 1 hit.
    InterProiIPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    [Graphical view]
    PfamiPF00378. ECH. 1 hit.
    [Graphical view]
    SUPFAMiSSF52096. SSF52096. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q08558-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSRVCYHIN GPFFIIKLID PKHLNSLTFE DFVYIALLLH KANDIDSVLF    50
    TVLQSSGKYF SSGGKFSAVN KLNDGDVTSE VEKVSKLVSA ISSPNIFVAN 100
    AFAIHKKVLV CCLNGPAIGL SASLVALCDI VYSQNDSVFL LFPFSNLGFV 150
    AEVGTSVTLT QKLGINSANE HMIFSTPVLF KELIGTIITK NYQLTNTETF 200
    NEKVLQDIKQ NLEGLYPKSV LGMKELLHSE MKQKLIKAQA METNGTLPFW 250
    ASGEPFKRFK QLQEGNRRHK L 271
    Length:271
    Mass (Da):30,058
    Last modified:November 1, 1996 - v1
    Checksum:i0AA0BF3A981F733F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z75088 Genomic DNA. Translation: CAA99389.1.
    AY558432 Genomic DNA. Translation: AAS56758.1.
    BK006948 Genomic DNA. Translation: DAA10952.1.
    PIRiS67072.
    RefSeqiNP_014823.3. NM_001183599.3.

    Genome annotation databases

    EnsemblFungiiYOR180C; YOR180C; YOR180C.
    GeneIDi854352.
    KEGGisce:YOR180C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z75088 Genomic DNA. Translation: CAA99389.1 .
    AY558432 Genomic DNA. Translation: AAS56758.1 .
    BK006948 Genomic DNA. Translation: DAA10952.1 .
    PIRi S67072.
    RefSeqi NP_014823.3. NM_001183599.3.

    3D structure databases

    ProteinModelPortali Q08558.
    SMRi Q08558. Positions 2-262.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34575. 21 interactions.
    DIPi DIP-4132N.
    IntActi Q08558. 2 interactions.
    MINTi MINT-386432.
    STRINGi 4932.YOR180C.

    Proteomic databases

    PaxDbi Q08558.
    PeptideAtlasi Q08558.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR180C ; YOR180C ; YOR180C .
    GeneIDi 854352.
    KEGGi sce:YOR180C.

    Organism-specific databases

    CYGDi YOR180c.
    SGDi S000005706. DCI1.

    Phylogenomic databases

    eggNOGi COG1024.
    GeneTreei ENSGT00670000097595.
    HOGENOMi HOG000027948.
    OMAi GQKKHKL.
    OrthoDBi EOG70PC7K.

    Enzyme and pathway databases

    UniPathwayi UPA00659 .
    BioCyci MetaCyc:YOR180C-MONOMER.
    YEAST:YOR180C-MONOMER.

    Miscellaneous databases

    NextBioi 976443.

    Gene expression databases

    Genevestigatori Q08558.

    Family and domain databases

    Gene3Di 3.90.226.10. 1 hit.
    InterProi IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    [Graphical view ]
    Pfami PF00378. ECH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52096. SSF52096. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Global regulatory functions of Oaf1p and Pip2p (Oaf2p), transcription factors that regulate genes encoding peroxisomal proteins in Saccharomyces cerevisiae."
      Karpichev I.V., Small G.M.
      Mol. Cell. Biol. 18:6560-6570(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    5. "Preliminary characterization of Yor180Cp: identification of a novel peroxisomal protein of Saccharomyces cerevisiae involved in fatty acid metabolism."
      Geisbrecht B.V., Schulz K., Nau K., Geraghty M.T., Schulz H., Erdmann R., Gould S.J.
      Biochem. Biophys. Res. Commun. 260:28-34(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH ECI1.
    6. "Alternatives to the isomerase-dependent pathway for the beta-oxidation of oleic acid are dispensable in Saccharomyces cerevisiae. Identification of YOR180c/DCI1 encoding peroxisomal delta(3,5)-delta(2,4)-dienoyl-CoA isomerase."
      Gurvitz A., Mursula A.M., Yagi A.I., Hartig A., Ruis H., Rottensteiner H., Hiltunen J.K.
      J. Biol. Chem. 274:24514-24521(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, SUBCELLULAR LOCATION, FUNCTION.
    7. "Evidence for a novel pathway for the targeting of a Saccharomyces cerevisiae peroxisomal protein belonging to the isomerase/hydratase family."
      Karpichev I.V., Small G.M.
      J. Cell Sci. 113:533-544(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, SUBCELLULAR LOCATION.
    8. "Eci1p uses a PTS1 to enter peroxisomes: either its own or that of a partner, Dci1p."
      Yang X., Purdue P.E., Lazarow P.B.
      Eur. J. Cell Biol. 80:126-138(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Ammonia pulses and metabolic oscillations guide yeast colony development."
      Palkova Z., Devaux F., Icicova M., Minarikova L., Le Crom S., Jacq C.
      Mol. Biol. Cell 13:3901-3914(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiDCI1_YEAST
    AccessioniPrimary (citable) accession number: Q08558
    Secondary accession number(s): D6W2N6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3