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Q08558

- DCI1_YEAST

UniProt

Q08558 - DCI1_YEAST

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Protein

Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase

Gene
DCI1, ECI2, EHD2, YOR180C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Converts 3,5-dienoyl-CoAs to the corresponding 2,4-dienoyl-CoAs. Involved in fatty acid metabolism. Required for ECI1 to be peroxisomal located.3 Publications

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei120 – 1201Substrate; via amide nitrogen By similarity
Sitei152 – 1521Important for catalytic activity By similarity

GO - Molecular functioni

  1. dodecenoyl-CoA delta-isomerase activity Source: SGD

GO - Biological processi

  1. fatty acid beta-oxidation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:YOR180C-MONOMER.
YEAST:YOR180C-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase (EC:5.3.3.-)
Gene namesi
Name:DCI1
Synonyms:ECI2, EHD2
Ordered Locus Names:YOR180C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR180c.
SGDiS000005706. DCI1.

Subcellular locationi

Peroxisome 3 Publications

GO - Cellular componenti

  1. peroxisomal matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerasePRO_0000232996Add
BLAST

Proteomic databases

PaxDbiQ08558.
PeptideAtlasiQ08558.

Expressioni

Inductioni

By oleate, through the binding of the OAF1-PIP2 transcriptional activator complex to its promoter ORE element. Expression is also induced during ammonia pulses in yeast colonies, at the beginning of detectable ammonia production.4 Publications

Gene expression databases

GenevestigatoriQ08558.

Interactioni

Subunit structurei

Interacts with ECI1.1 Publication

Protein-protein interaction databases

BioGridi34575. 21 interactions.
DIPiDIP-4132N.
IntActiQ08558. 2 interactions.
MINTiMINT-386432.
STRINGi4932.YOR180C.

Structurei

3D structure databases

ProteinModelPortaliQ08558.
SMRiQ08558. Positions 2-262.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni62 – 665Substrate binding By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi269 – 2713Microbody targeting signal Reviewed prediction

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1024.
GeneTreeiENSGT00670000097595.
HOGENOMiHOG000027948.
OMAiGQKKHKL.
OrthoDBiEOG70PC7K.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
[Graphical view]
PfamiPF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 1 hit.

Sequencei

Sequence statusi: Complete.

Q08558-1 [UniParc]FASTAAdd to Basket

« Hide

MSSRVCYHIN GPFFIIKLID PKHLNSLTFE DFVYIALLLH KANDIDSVLF    50
TVLQSSGKYF SSGGKFSAVN KLNDGDVTSE VEKVSKLVSA ISSPNIFVAN 100
AFAIHKKVLV CCLNGPAIGL SASLVALCDI VYSQNDSVFL LFPFSNLGFV 150
AEVGTSVTLT QKLGINSANE HMIFSTPVLF KELIGTIITK NYQLTNTETF 200
NEKVLQDIKQ NLEGLYPKSV LGMKELLHSE MKQKLIKAQA METNGTLPFW 250
ASGEPFKRFK QLQEGNRRHK L 271
Length:271
Mass (Da):30,058
Last modified:November 1, 1996 - v1
Checksum:i0AA0BF3A981F733F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z75088 Genomic DNA. Translation: CAA99389.1.
AY558432 Genomic DNA. Translation: AAS56758.1.
BK006948 Genomic DNA. Translation: DAA10952.1.
PIRiS67072.
RefSeqiNP_014823.3. NM_001183599.3.

Genome annotation databases

EnsemblFungiiYOR180C; YOR180C; YOR180C.
GeneIDi854352.
KEGGisce:YOR180C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z75088 Genomic DNA. Translation: CAA99389.1 .
AY558432 Genomic DNA. Translation: AAS56758.1 .
BK006948 Genomic DNA. Translation: DAA10952.1 .
PIRi S67072.
RefSeqi NP_014823.3. NM_001183599.3.

3D structure databases

ProteinModelPortali Q08558.
SMRi Q08558. Positions 2-262.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34575. 21 interactions.
DIPi DIP-4132N.
IntActi Q08558. 2 interactions.
MINTi MINT-386432.
STRINGi 4932.YOR180C.

Proteomic databases

PaxDbi Q08558.
PeptideAtlasi Q08558.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR180C ; YOR180C ; YOR180C .
GeneIDi 854352.
KEGGi sce:YOR180C.

Organism-specific databases

CYGDi YOR180c.
SGDi S000005706. DCI1.

Phylogenomic databases

eggNOGi COG1024.
GeneTreei ENSGT00670000097595.
HOGENOMi HOG000027948.
OMAi GQKKHKL.
OrthoDBi EOG70PC7K.

Enzyme and pathway databases

UniPathwayi UPA00659 .
BioCyci MetaCyc:YOR180C-MONOMER.
YEAST:YOR180C-MONOMER.

Miscellaneous databases

NextBioi 976443.

Gene expression databases

Genevestigatori Q08558.

Family and domain databases

Gene3Di 3.90.226.10. 1 hit.
InterProi IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
[Graphical view ]
Pfami PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF52096. SSF52096. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Global regulatory functions of Oaf1p and Pip2p (Oaf2p), transcription factors that regulate genes encoding peroxisomal proteins in Saccharomyces cerevisiae."
    Karpichev I.V., Small G.M.
    Mol. Cell. Biol. 18:6560-6570(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  5. "Preliminary characterization of Yor180Cp: identification of a novel peroxisomal protein of Saccharomyces cerevisiae involved in fatty acid metabolism."
    Geisbrecht B.V., Schulz K., Nau K., Geraghty M.T., Schulz H., Erdmann R., Gould S.J.
    Biochem. Biophys. Res. Commun. 260:28-34(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH ECI1.
  6. "Alternatives to the isomerase-dependent pathway for the beta-oxidation of oleic acid are dispensable in Saccharomyces cerevisiae. Identification of YOR180c/DCI1 encoding peroxisomal delta(3,5)-delta(2,4)-dienoyl-CoA isomerase."
    Gurvitz A., Mursula A.M., Yagi A.I., Hartig A., Ruis H., Rottensteiner H., Hiltunen J.K.
    J. Biol. Chem. 274:24514-24521(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, SUBCELLULAR LOCATION, FUNCTION.
  7. "Evidence for a novel pathway for the targeting of a Saccharomyces cerevisiae peroxisomal protein belonging to the isomerase/hydratase family."
    Karpichev I.V., Small G.M.
    J. Cell Sci. 113:533-544(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, SUBCELLULAR LOCATION.
  8. "Eci1p uses a PTS1 to enter peroxisomes: either its own or that of a partner, Dci1p."
    Yang X., Purdue P.E., Lazarow P.B.
    Eur. J. Cell Biol. 80:126-138(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Ammonia pulses and metabolic oscillations guide yeast colony development."
    Palkova Z., Devaux F., Icicova M., Minarikova L., Le Crom S., Jacq C.
    Mol. Biol. Cell 13:3901-3914(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiDCI1_YEAST
AccessioniPrimary (citable) accession number: Q08558
Secondary accession number(s): D6W2N6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

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