Q08558 (DCI1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase
EC=5.3.3.-

Gene names

Name:	DCI1
Synonyms:	ECI2, EHD2
Ordered Locus Names:	YOR180C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts 3,5-dienoyl-CoAs to the corresponding 2,4-dienoyl-CoAs. Involved in fatty acid metabolism. Required for ECI1 to be peroxisomal located. Ref.5 Ref.6 Ref.8
Pathway	Lipid metabolism; fatty acid beta-oxidation.
Subunit structure	Interacts with ECI1. Ref.5
Subcellular location	Peroxisome Ref.5 Ref.6 Ref.7.
Induction	By oleate, through the binding of the OAF1-PIP2 transcriptional activator complex to its promoter ORE element. Expression is also induced during ammonia pulses in yeast colonies, at the beginning of detectable ammonia production. Ref.4 Ref.6 Ref.7 Ref.9
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Peroxisome
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid beta-oxidation Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	peroxisomal matrix Inferred from direct assay Ref.5. Source: SGD
Molecular_function	dodecenoyl-CoA delta-isomerase activity Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase		PRO_0000232996
Regions
	Region	62 – 66	5	Substrate binding By similarity
	Motif	269 – 271	3	Microbody targeting signal Potential
Sites
	Binding site	120	1	Substrate; via amide nitrogen By similarity
	Site	152	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q08558 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0AA0BF3A981F733F	FASTA	271	30,058
	10 20 30 40 50 60 MSSRVCYHIN GPFFIIKLID PKHLNSLTFE DFVYIALLLH KANDIDSVLF TVLQSSGKYF 70 80 90 100 110 120 SSGGKFSAVN KLNDGDVTSE VEKVSKLVSA ISSPNIFVAN AFAIHKKVLV CCLNGPAIGL 130 140 150 160 170 180 SASLVALCDI VYSQNDSVFL LFPFSNLGFV AEVGTSVTLT QKLGINSANE HMIFSTPVLF 190 200 210 220 230 240 KELIGTIITK NYQLTNTETF NEKVLQDIKQ NLEGLYPKSV LGMKELLHSE MKQKLIKAQA 250 260 270 METNGTLPFW ASGEPFKRFK QLQEGNRRHK L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The reference genome sequence of Saccharomyces cerevisiae: Then and now." Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M. G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global regulatory functions of Oaf1p and Pip2p (Oaf2p), transcription factors that regulate genes encoding peroxisomal proteins in Saccharomyces cerevisiae." Karpichev I.V., Small G.M. Mol. Cell. Biol. 18:6560-6570(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[5]	"Preliminary characterization of Yor180Cp: identification of a novel peroxisomal protein of Saccharomyces cerevisiae involved in fatty acid metabolism." Geisbrecht B.V., Schulz K., Nau K., Geraghty M.T., Schulz H., Erdmann R., Gould S.J. Biochem. Biophys. Res. Commun. 260:28-34(1999) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH ECI1.
[6]	"Alternatives to the isomerase-dependent pathway for the beta-oxidation of oleic acid are dispensable in Saccharomyces cerevisiae. Identification of YOR180c/DCI1 encoding peroxisomal delta(3,5)-delta(2,4)-dienoyl-CoA isomerase." Gurvitz A., Mursula A.M., Yagi A.I., Hartig A., Ruis H., Rottensteiner H., Hiltunen J.K. J. Biol. Chem. 274:24514-24521(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, SUBCELLULAR LOCATION, FUNCTION.
[7]	"Evidence for a novel pathway for the targeting of a Saccharomyces cerevisiae peroxisomal protein belonging to the isomerase/hydratase family." Karpichev I.V., Small G.M. J. Cell Sci. 113:533-544(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, SUBCELLULAR LOCATION.
[8]	"Eci1p uses a PTS1 to enter peroxisomes: either its own or that of a partner, Dci1p." Yang X., Purdue P.E., Lazarow P.B. Eur. J. Cell Biol. 80:126-138(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Ammonia pulses and metabolic oscillations guide yeast colony development." Palkova Z., Devaux F., Icicova M., Minarikova L., Le Crom S., Jacq C. Mol. Biol. Cell 13:3901-3914(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z75088 Genomic DNA. Translation: CAA99389.1. AY558432 Genomic DNA. Translation: AAS56758.1. BK006948 Genomic DNA. Translation: DAA10952.1.
PIR	S67072.
RefSeq	NP_014823.3. NM_001183599.3.
3D structure databases
ProteinModelPortal	Q08558.
SMR	Q08558. Positions 2-262.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	34575. 21 interactions.
DIP	DIP-4132N.
IntAct	Q08558. 2 interactions.
MINT	MINT-386432.
STRING	4932.YOR180C.
Proteomic databases
PaxDb	Q08558.
PeptideAtlas	Q08558.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR180C; YOR180C; YOR180C.
GeneID	854352.
KEGG	sce:YOR180C.
Organism-specific databases
CYGD	YOR180c.
SGD	S000005706. DCI1.
Phylogenomic databases
eggNOG	COG1024.
GeneTree	ENSGT00670000097595.
HOGENOM	HOG000027948.
OMA	GQKKHKL.
OrthoDB	EOG70PC7K.
Enzyme and pathway databases
BioCyc	MetaCyc:YOR180C-MONOMER. YEAST:YOR180C-MONOMER.
UniPathway	UPA00659.
Gene expression databases
Genevestigator	Q08558.
Family and domain databases
Gene3D	3.90.226.10. 1 hit.
InterPro	IPR029045. ClpP/crotonase-like_dom. IPR001753. Crotonase_core_superfam. [Graphical view]
Pfam	PF00378. ECH. 1 hit. [Graphical view]
SUPFAM	SSF52096. SSF52096. 1 hit.
ProtoNet	Search...
Other
NextBio	976443.

Entry information

Entry name

DCI1_YEAST

Accession

Primary (citable) accession number: Q08558
Secondary accession number(s): D6W2N6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	November 1, 1996
Last modified:	June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program