Reviewed, UniProtKB/Swiss-Prot Q08554 (DSC1_HUMAN)

Names and origin

Protein names

Recommended name:
    Desmocollin-1
Alternative name(s):
    Desmosomal glycoprotein 2/3
    DG2/DG3

Gene names

Name:	DSC1
Synonyms:	CDHF1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	894 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. Linked to the keratinization of epithelial tissues.
Subcellular location	Cell membrane; Single-pass type I membrane protein. Cell junction › desmosome.
Tissue specificity	Strongly expressed in epidermis, less in lymph node and tongue.
Domain	Calcium may be bound by the cadherin-like repeats Potential.
Sequence similarities	Contains 5 cadherin domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell junction Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal Transmembrane
Ligand	Calcium
PTM	Cleavage on pair of basic residues Glycoprotein Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	homophilic cell adhesion Inferred from electronic annotation. Source: InterPro
Cellular component	gap junction Traceable author statement. Source: ProtInc integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane fraction Traceable author statement. Source: ProtInc
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1A (identifier: Q08554-1)

Also known as: DG2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1B (identifier: Q08554-2)

Also known as: DG3;

The sequence of this isoform differs from the canonical sequence as follows:
     830-840: KVYLCGQDEEH → ESIRGHTLIKN
     841-894: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Propeptide	30 – 134	105	Potential		PRO_0000003863
	Chain	135 – 894	760	Desmocollin-1		PRO_0000003864
Regions
	Topological domain	135 – 691	557	Extracellular Potential
	Transmembrane	692 – 714	23	Potential
	Topological domain	715 – 894	180	Cytoplasmic Potential
	Domain	135 – 242	108	Cadherin 1
	Domain	243 – 354	112	Cadherin 2
	Domain	355 – 471	117	Cadherin 3
	Domain	472 – 575	104	Cadherin 4
	Domain	576 – 682	107	Cadherin 5
Amino acid modifications
	Modified residue	385	1	Phosphothreonine
	Glycosylation	165	1	N-linked (GlcNAc...) Potential
	Glycosylation	546	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	830 – 840	11	KVYLCGQDEEH → ESIRGHTLIKN in isoform 1B.		VSP_000651
	Alternative sequence	841 – 894	54	Missing in isoform 1B.		VSP_000652
Experimental info
	Sequence conflict	132	1	T → S in CAA51428 and CAA51429. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1A (DG2) [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 44BA33038699E3E1	FASTA	894	100,045
	10 20 30 40 50 60 MALASAAPGS IFCKQLLFSL LVLTLLCDAC QKVYLRVPSH LQAETLVGKV NLEECLKSAS 70 80 90 100 110 120 LIRSSDPAFR ILEDGSIYTT HDLILSSERK SFSIFLSDGQ RREQQEIKVV LSARENKSPK 130 140 150 160 170 180 KRHTKDTALK RTKRRWAPIP ASLMENSLGP FPQHVQQIQS DAAQNYTIFY SISGPGVDKE 190 200 210 220 230 240 PFNLFYIEKD TGDIFCTRSI DREKYEQFAL YGYATTADGY APEYPLPLII KIEDDNDNAP 250 260 270 280 290 300 YFEHRVTIFT VPENCRSGTS VGKVTATDLD EPDTLHTRLK YKILQQIPDH PKHFSIHPDT 310 320 330 340 350 360 GVITTTTPFL DREKCDTYQL IMEVRDMGGQ PFGLFNTGTI TISLEDENDN PPSFTETSYV 370 380 390 400 410 420 TEVEENRIDV EILRMKVQDQ DLPNTPHSKA VYKILQGNEN GNFIISTDPN TNEGVLCVVK 430 440 450 460 470 480 PLNYEVNRQV ILQVGVINEA QFSKAASSQT PTMCTTTVTV KIIDSDEGPE CHPPVKVIQS 490 500 510 520 530 540 QDGFPAGQEL LGYKALDPEI SSGEGLRYQK LGDEDNWFEI NQHTGDLRTL KVLDRESKFV 550 560 570 580 590 600 KNNQYNISVV AVDAVGRSCT GTLVVHLDDY NDHAPQIDKE VTICQNNEDF AVLKPVDPDG 610 620 630 640 650 660 PENGPPFQFF LDNSASKNWN IEEKDGKTAI LRQRQNLDYN YYSVPIQIKD RHGLVATHML 670 680 690 700 710 720 TVRVCDCSTP SECRMKDKST RDVRPNVILG RWAILAMVLG SVLLLCILFT CFCVTAKRTV 730 740 750 760 770 780 KKCFPEDIAQ QNLIVSNTEG PGEEVTEANI RLPMQTSNIC DTSMSVGTVG GQGIKTQQSF 790 800 810 820 830 840 EMVKGGYTLD SNKGGGHQTL ESVKGVGQGD TGRYAYTDWQ SFTQPRLGEK VYLCGQDEEH 850 860 870 880 890 KHCEDYVFSY NYEGKGSLAG SVGCCSDRQE EEGLEFLDHL EPKFRTLAKT CIKK
	Isoform 1B (DG3) [UniParc]. Checksum: 702473A68425A45F		840	93,849
	10 20 30 40 50 60 MALASAAPGS IFCKQLLFSL LVLTLLCDAC QKVYLRVPSH LQAETLVGKV NLEECLKSAS 70 80 90 100 110 120 LIRSSDPAFR ILEDGSIYTT HDLILSSERK SFSIFLSDGQ RREQQEIKVV LSARENKSPK 130 140 150 160 170 180 KRHTKDTALK RTKRRWAPIP ASLMENSLGP FPQHVQQIQS DAAQNYTIFY SISGPGVDKE 190 200 210 220 230 240 PFNLFYIEKD TGDIFCTRSI DREKYEQFAL YGYATTADGY APEYPLPLII KIEDDNDNAP 250 260 270 280 290 300 YFEHRVTIFT VPENCRSGTS VGKVTATDLD EPDTLHTRLK YKILQQIPDH PKHFSIHPDT 310 320 330 340 350 360 GVITTTTPFL DREKCDTYQL IMEVRDMGGQ PFGLFNTGTI TISLEDENDN PPSFTETSYV 370 380 390 400 410 420 TEVEENRIDV EILRMKVQDQ DLPNTPHSKA VYKILQGNEN GNFIISTDPN TNEGVLCVVK 430 440 450 460 470 480 PLNYEVNRQV ILQVGVINEA QFSKAASSQT PTMCTTTVTV KIIDSDEGPE CHPPVKVIQS 490 500 510 520 530 540 QDGFPAGQEL LGYKALDPEI SSGEGLRYQK LGDEDNWFEI NQHTGDLRTL KVLDRESKFV 550 560 570 580 590 600 KNNQYNISVV AVDAVGRSCT GTLVVHLDDY NDHAPQIDKE VTICQNNEDF AVLKPVDPDG 610 620 630 640 650 660 PENGPPFQFF LDNSASKNWN IEEKDGKTAI LRQRQNLDYN YYSVPIQIKD RHGLVATHML 670 680 690 700 710 720 TVRVCDCSTP SECRMKDKST RDVRPNVILG RWAILAMVLG SVLLLCILFT CFCVTAKRTV 730 740 750 760 770 780 KKCFPEDIAQ QNLIVSNTEG PGEEVTEANI RLPMQTSNIC DTSMSVGTVG GQGIKTQQSF 790 800 810 820 830 840 EMVKGGYTLD SNKGGGHQTL ESVKGVGQGD TGRYAYTDWQ SFTQPRLGEE SIRGHTLIKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differential synthesis of type 1 and type 2 desmocollin mRNAs in human stratified epithelia." Theis D.G., Koch P.J., Franke W.W. Int. J. Dev. Biol. 37:101-110(1993) [PubMed: 8507556] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Foreskin.
[2]	Zimbelmann R. Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Foreskin.
[3]	"Cloning of the cDNA (DSC1) coding for human type 1 desmocollin and its assignment to chromosome 18." King I.A., Arnemann J., Spurr N.K., Buxton R.S. Genomics 18:185-194(1993) [PubMed: 8288219] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skin.
[4]	"Keratinization is associated with the expression of a new protein related to the desmosomal cadherins DGII/III." King I.A., Magee A.I., Rees D.A., Buxton R.S. FEBS Lett. 286:9-12(1991) [PubMed: 1713860] [Abstract] Cited for: PROTEIN SEQUENCE OF 135-151 AND 283-292.
[5]	"Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line." Giorgianni F., Zhao Y., Desiderio D.M., Beranova-Giorgianni S. Electrophoresis 28:2027-2034(2007) [PubMed: 17487921] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-385, MASS SPECTROMETRY.
[6]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-385, MASS SPECTROMETRY. Tissue: Epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z34522 mRNA. Translation: CAA84279.1. Z34522 mRNA. Translation: CAA84278.1. X72925 mRNA. Translation: CAA51428.1. X72925 mRNA. Translation: CAA51429.1.
PIR	I37281. I37282.
UniGene	Hs.567260
3D structure databases
HSSP	HSSP built from PDB template 1NCJ based on UniProtKB P15116.
ModBase	Search...
PTM databases
PhosphoSite	Q08554.
Genome annotation databases
Ensembl	ENSG00000134765. Homo sapiens. [Contig view]
Organism-specific databases
H-InvDB	HIX0039739.
HGNC	HGNC:3035. DSC1.
HPA	HPA012891.
MIM	125643. gene.
PharmGKB	PA27488.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q08554.
HOVERGEN	Q08554.
Gene expression databases
ArrayExpress	Q08554.
CleanEx	HS_DSC1.
GermOnline	ENSG00000134765. Homo sapiens.
Family and domain databases
InterPro	IPR002126. Cadherin. IPR014868. Cadherin_pro. IPR009122. Desmo_cadherin. IPR009124. Desmocollin. [Graphical view]
Gene3D	G3DSA:2.60.40.60. Cadherin. 4 hits.
Pfam	PF00028. Cadherin. 5 hits. PF08758. Cadherin_pro. 1 hit. [Graphical view]
PRINTS	PR00205. CADHERIN. PR01818. DESMOCADHERN. PR01820. DESMOCOLLIN.
SMART	SM00112. CA. 5 hits. [Graphical view]
PROSITE	PS00232. CADHERIN_1. 3 hits. PS50268. CADHERIN_2. 5 hits. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

DSC1_HUMAN

Accession

Primary (citable) accession number: Q08554

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)