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Protein

Lysophospholipid acyltransferase

Gene

ALE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Membrane-bound O-acyltransferase that mediates the incorporation of unsaturated acyl chains into the sn-2 position of phospholipids. Preferentially acylates lysophosphocholine, but also lysophosphoethanolamine and lysophosphatidylglycerol.4 Publications

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
Acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine = CoA + 1,2-diacyl-sn-glycero-3-phosphocholine.
Acyl-CoA + 1-acyl-sn-glycero-3-phosphoethanolamine = CoA + 1,2-diacyl-sn-glycero-3-phosphoethanolamine.

Kineticsi

  1. KM=10 µM for oleoyl-CoA (with lysophosphoethanolamine as cosubstrate)
  2. KM=17 µM for palmitoleoyl-CoA (with lysophosphoethanolamine as cosubstrate)
  3. KM=0.9 µM for palmitoyl-CoA (with lysophosphoethanolamine as cosubstrate)
  4. KM=0.4 µM for myristoyl-CoA (with lysophosphoethanolamine as cosubstrate)
  5. KM=49 µM for oleoyl-CoA (with lysophosphocholine as cosubstrate)
  6. KM=21 µM for palmitoleoyl-CoA (with lysophosphocholine as cosubstrate)
  7. KM=1.8 µM for palmitoyl-CoA (with lysophosphocholine as cosubstrate)
  8. KM=5.9 µM for stearoyl-CoA (with lysophosphocholine as cosubstrate)
  9. KM=11 µM for arachidonyl-CoA (with lysophosphocholine as cosubstrate)
  1. Vmax=38 nmol/min/mg enzyme with oleoyl-CoA as substrate (with lysophosphoethanolamine as cosubstrate)
  2. Vmax=44 nmol/min/mg enzyme with palmitoleoyl-CoA as substrate (with lysophosphoethanolamine as substrate)
  3. Vmax=3.7 nmol/min/mg enzyme with palmitoyl-CoA as substrate (with lysophosphoethanolamine as substrate)
  4. Vmax=1.2 nmol/min/mg enzyme with myristoyl-CoA as substrate (with lysophosphoethanolamine as substrate)
  5. Vmax=125 nmol/min/mg enzyme with oleoyl-CoA as substrate (with lysophosphocholine as substrate)
  6. Vmax=142 nmol/min/mg enzyme with palmitoleoyl-CoA as substrate (with lysophosphocholine as substrate)
  7. Vmax=7.8 nmol/min/mg enzyme with palmitoyl-CoA as substrate (with lysophosphocholine as substrate)
  8. Vmax=12 nmol/min/mg enzyme with stearoyl-CoA as substrate (with lysophosphocholine as substrate)
  9. Vmax=58 nmol/min/mg enzyme with arachidonyl-CoA as substrate (with lysophosphocholine as substrate)

pH dependencei

Optimum pH is 6.5-7.5.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei382Sequence analysis1

GO - Molecular functioni

  • 1-acylglycerol-3-phosphate O-acyltransferase activity Source: SGD
  • 1-acylglycerophosphocholine O-acyltransferase activity Source: SGD
  • O-acyltransferase activity Source: SGD

GO - Biological processi

  • glycerophospholipid biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:G3O-33688-MONOMER.
YEAST:G3O-33688-MONOMER.
BRENDAi2.3.1.51. 984.
ReactomeiR-SCE-1482788. Acyl chain remodelling of PC.
R-SCE-1482801. Acyl chain remodelling of PS.
R-SCE-1482839. Acyl chain remodelling of PE.
R-SCE-1482922. Acyl chain remodelling of PI.
SABIO-RKQ08548.

Chemistry databases

SwissLipidsiSLP:000000083.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipid acyltransferase (EC:2.3.1.23, EC:2.3.1.51)
Short name:
LPLAT
Alternative name(s):
1-acyl-sn-glycerol-3-phosphate acyltransferase
Short name:
AGPAT
Lysophosphatidic acid acyltransferase
Short name:
LPAAT
Lysophosphatidylcholine acyltransferase
Short name:
LPCAT
Lysophosphatidylethanolamine acyltransferase
Short name:
LPEAT
Gene namesi
Name:ALE1
Synonyms:LCA1, LPT1, SLC4
Ordered Locus Names:YOR175C
ORF Names:O3635
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR175C.
SGDiS000005701. ALE1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 19LumenalSequence analysisAdd BLAST19
Transmembranei20 – 39HelicalSequence analysisAdd BLAST20
Topological domaini40 – 51CytoplasmicSequence analysisAdd BLAST12
Transmembranei52 – 72HelicalSequence analysisAdd BLAST21
Topological domaini73 – 92LumenalSequence analysisAdd BLAST20
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Topological domaini114 – 231CytoplasmicSequence analysisAdd BLAST118
Transmembranei232 – 252HelicalSequence analysisAdd BLAST21
Topological domaini253 – 274LumenalSequence analysisAdd BLAST22
Transmembranei275 – 295HelicalSequence analysisAdd BLAST21
Topological domaini296 – 429CytoplasmicSequence analysisAdd BLAST134
Transmembranei430 – 450HelicalSequence analysisAdd BLAST21
Topological domaini451 – 456LumenalSequence analysis6
Transmembranei457 – 477HelicalSequence analysisAdd BLAST21
Topological domaini478 – 619CytoplasmicSequence analysisAdd BLAST142

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002452571 – 619Lysophospholipid acyltransferaseAdd BLAST619

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei513PhosphoserineCombined sources1
Modified residuei605PhosphoserineCombined sources1
Modified residuei610PhosphoserineCombined sources1
Modified residuei615PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08548.
PRIDEiQ08548.

PTM databases

iPTMnetiQ08548.

Interactioni

Protein-protein interaction databases

BioGridi34569. 30 interactors.
DIPiDIP-5617N.
MINTiMINT-570086.

Structurei

3D structure databases

ProteinModelPortaliQ08548.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili545 – 593Sequence analysisAdd BLAST49

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074565.
HOGENOMiHOG000200782.
InParanoidiQ08548.
KOiK13519.
OMAiECLEAWN.
OrthoDBiEOG092C26BD.

Family and domain databases

InterProiIPR004299. MBOAT_fam.
[Graphical view]
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08548-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNPVDAVLT KIITNYGIDS FTLRYAICLL GSFPLNAILK RIPEKRIGLK
60 70 80 90 100
CCFIISMSMF YLFGVLNLVS GFRTLFISTM FTYLISRFYR SKFMPHLNFM
110 120 130 140 150
FVMGHLAINH IHAQFLNEQT QTTVDITSSQ MVLAMKLTSF AWSYYDGSCT
160 170 180 190 200
SESDFKDLTE HQKSRAVRGH PPLLKFLAYA FFYSTLLTGP SFDYADFDSW
210 220 230 240 250
LNCEMFRDLP ESKKPMRRHH PGERRQIPKN GKLALWKVVQ GLAWMILSTL
260 270 280 290 300
GMKHFPVKYV LDKDGFPTRS FIFRIHYLFL LGFIHRFKYY AAWTISEGSC
310 320 330 340 350
ILCGLGYNGY DSKTQKIRWD RVRNIDIWTV ETAQNTREML EAWNMNTNKW
360 370 380 390 400
LKYSVYLRVT KKGKKPGFRS TLFTFLTSAF WHGTRPGYYL TFATGALYQT
410 420 430 440 450
CGKIYRRNFR PIFLREDGVT PLPSKKIYDL VGIYAIKLAF GYMVQPFIIL
460 470 480 490 500
DLKPSLMVWG SVYFYVHIIV AFSFFLFRGP YAKQVTEFFK SKQPKEIFIR
510 520 530 540 550
KQKKLEKDIS ASSPNLGGIL KAKIEHEKGK TAEEEEMNLG IPPIELEKWD
560 570 580 590 600
NAKEDWEDFC KDYKEWRNKN GLEIEEENLS KAFERFKQEF SNAASGSGER
610
VRKMSFSGYS PKPISKKEE
Length:619
Mass (Da):72,228
Last modified:November 1, 1996 - v1
Checksum:i87C74C9194BE0BC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75083 Genomic DNA. Translation: CAA99384.1.
U55021 Genomic DNA. Translation: AAB47420.1.
BK006948 Genomic DNA. Translation: DAA10947.1.
PIRiS67067.
RefSeqiNP_014818.1. NM_001183594.1.

Genome annotation databases

EnsemblFungiiYOR175C; YOR175C; YOR175C.
GeneIDi854346.
KEGGisce:YOR175C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75083 Genomic DNA. Translation: CAA99384.1.
U55021 Genomic DNA. Translation: AAB47420.1.
BK006948 Genomic DNA. Translation: DAA10947.1.
PIRiS67067.
RefSeqiNP_014818.1. NM_001183594.1.

3D structure databases

ProteinModelPortaliQ08548.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34569. 30 interactors.
DIPiDIP-5617N.
MINTiMINT-570086.

Chemistry databases

SwissLipidsiSLP:000000083.

PTM databases

iPTMnetiQ08548.

Proteomic databases

MaxQBiQ08548.
PRIDEiQ08548.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR175C; YOR175C; YOR175C.
GeneIDi854346.
KEGGisce:YOR175C.

Organism-specific databases

EuPathDBiFungiDB:YOR175C.
SGDiS000005701. ALE1.

Phylogenomic databases

GeneTreeiENSGT00550000074565.
HOGENOMiHOG000200782.
InParanoidiQ08548.
KOiK13519.
OMAiECLEAWN.
OrthoDBiEOG092C26BD.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-33688-MONOMER.
YEAST:G3O-33688-MONOMER.
BRENDAi2.3.1.51. 984.
ReactomeiR-SCE-1482788. Acyl chain remodelling of PC.
R-SCE-1482801. Acyl chain remodelling of PS.
R-SCE-1482839. Acyl chain remodelling of PE.
R-SCE-1482922. Acyl chain remodelling of PI.
SABIO-RKQ08548.

Miscellaneous databases

PROiQ08548.

Family and domain databases

InterProiIPR004299. MBOAT_fam.
[Graphical view]
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALE1_YEAST
AccessioniPrimary (citable) accession number: Q08548
Secondary accession number(s): D6W2N1, Q03130
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.