##gff-version 3
Q08509	UniProtKB	Chain	1	821	.	.	.	ID=PRO_0000086995;Note=Epidermal growth factor receptor kinase substrate 8	
Q08509	UniProtKB	Domain	64	194	.	.	.	Note=PTB;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q08509	UniProtKB	Domain	530	589	.	.	.	Note=SH3;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00192	
Q08509	UniProtKB	Region	1	39	.	.	.	Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
Q08509	UniProtKB	Region	204	224	.	.	.	Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
Q08509	UniProtKB	Region	295	320	.	.	.	Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
Q08509	UniProtKB	Region	461	525	.	.	.	Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
Q08509	UniProtKB	Region	610	683	.	.	.	Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
Q08509	UniProtKB	Region	648	821	.	.	.	Note=Effector region	
Q08509	UniProtKB	Region	679	697	.	.	.	Note=Amphipathic helix	
Q08509	UniProtKB	Region	717	737	.	.	.	Note=Helix bundle 1	
Q08509	UniProtKB	Region	751	756	.	.	.	Note=Helix bundle 2	
Q08509	UniProtKB	Region	761	766	.	.	.	Note=Helix bundle 3	
Q08509	UniProtKB	Region	765	784	.	.	.	Note=Helix bundle 4	
Q08509	UniProtKB	Region	800	821	.	.	.	Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
Q08509	UniProtKB	Compositional bias	1	34	.	.	.	Note=Polar residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
Q08509	UniProtKB	Compositional bias	206	220	.	.	.	Note=Pro residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
Q08509	UniProtKB	Compositional bias	463	477	.	.	.	Note=Basic and acidic residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
Q08509	UniProtKB	Compositional bias	620	645	.	.	.	Note=Pro residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
Q08509	UniProtKB	Compositional bias	650	670	.	.	.	Note=Polar residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
Q08509	UniProtKB	Modified residue	58	58	.	.	.	Note=Phosphoserine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:F1M3L7	
Q08509	UniProtKB	Modified residue	223	223	.	.	.	Note=Phosphothreonine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q12929	
Q08509	UniProtKB	Modified residue	317	317	.	.	.	Note=Phosphothreonine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:21183079;Dbxref=PMID:21183079	
Q08509	UniProtKB	Modified residue	475	475	.	.	.	Note=Phosphoserine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q12929	
Q08509	UniProtKB	Modified residue	624	624	.	.	.	Note=Phosphoserine%3B by MAPK;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19564905;Dbxref=PMID:19564905	
Q08509	UniProtKB	Modified residue	628	628	.	.	.	Note=Phosphothreonine%3B by MAPK;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19564905;Dbxref=PMID:19564905	
Q08509	UniProtKB	Modified residue	658	658	.	.	.	Note=Phosphoserine;Ontology_term=ECO:0007744,ECO:0007744;evidence=ECO:0007744|PubMed:19144319,ECO:0007744|PubMed:21183079;Dbxref=PMID:19144319,PMID:21183079	
Q08509	UniProtKB	Modified residue	661	661	.	.	.	Note=Phosphoserine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:21183079;Dbxref=PMID:21183079	
Q08509	UniProtKB	Modified residue	684	684	.	.	.	Note=Phosphoserine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:21183079;Dbxref=PMID:21183079	
Q08509	UniProtKB	Modified residue	810	810	.	.	.	Note=Phosphoserine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:21183079;Dbxref=PMID:21183079	
Q08509	UniProtKB	Modified residue	814	814	.	.	.	Note=Phosphoserine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:21183079;Dbxref=PMID:21183079	
Q08509	UniProtKB	Mutagenesis	624	624	.	.	.	Note=Does not detach from actin following BDNF treatment%3B when associated with A-628. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19564905;Dbxref=PMID:19564905	
Q08509	UniProtKB	Mutagenesis	624	624	.	.	.	Note=Mimicks phosphorylation state%3B promotes detachment from actin in absence of BDNF treatment%3B when associated with E-628. S->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19564905;Dbxref=PMID:19564905	
Q08509	UniProtKB	Mutagenesis	628	628	.	.	.	Note=Does not detach from actin following BDNF treatment%3B when associated with A-624. T->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19564905;Dbxref=PMID:19564905	
Q08509	UniProtKB	Mutagenesis	628	628	.	.	.	Note=Mimicks phosphorylation state%3B promotes detachment from actin in absence of BDNF treatment%3B when associated with E-624. T->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19564905;Dbxref=PMID:19564905	
Q08509	UniProtKB	Mutagenesis	689	689	.	.	.	Note=Abolishes barbed-end actin-binding without affecting actin bundling activity. V->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20532239;Dbxref=PMID:20532239	
Q08509	UniProtKB	Mutagenesis	693	693	.	.	.	Note=Abolishes barbed-end actin-binding without affecting actin bundling activity. L->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20532239;Dbxref=PMID:20532239	
Q08509	UniProtKB	Mutagenesis	706	706	.	.	.	Note=Impairs both actin capping and bundling activities. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20532239;Dbxref=PMID:20532239	
Q08509	UniProtKB	Mutagenesis	708	708	.	.	.	Note=Impairs both actin capping and bundling activities. F->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20532239;Dbxref=PMID:20532239	
Q08509	UniProtKB	Sequence conflict	652	652	.	.	.	Note=N->D;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q08509	UniProtKB	Beta strand	534	539	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1I07	
Q08509	UniProtKB	Beta strand	556	559	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1I07	
Q08509	UniProtKB	Helix	561	563	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1I07	
Q08509	UniProtKB	Beta strand	566	570	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1I07	
Q08509	UniProtKB	Beta strand	576	580	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1I07	
Q08509	UniProtKB	Helix	581	583	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1I07	
Q08509	UniProtKB	Beta strand	584	587	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1I07