Epidermal growth factor receptor kinase substrate 8

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Reviewed, UniProtKB/Swiss-Prot Q08509 (EPS8_MOUSE)

Last modified June 16, 2009. Version 83. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Epidermal growth factor receptor kinase substrate 8

Gene names

Name:

Eps8

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	821 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Upon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets.
Subunit structure	Homodimer. Interacts with BAIAP2 By similarity. Interacts with SHB.
Domain	The SH3 domain mediates interaction with SHB By similarity.
Post-translational modification	Phosphorylated by several receptor tyrosine kinases.
Sequence similarities	Belongs to the EPS8 family. Contains 1 PH domain. Contains 1 SH3 domain.

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Ontologies

Keywords
Domain	SH3 domain
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	actin cytoskeleton reorganization Inferred from mutant phenotype. Source: MGI adult locomotory behavior Inferred from mutant phenotype. Source: MGI behavioral response to ethanol Inferred from mutant phenotype. Source: MGI
Cellular component	N-methyl-D-aspartate selective glutamate receptor complex Inferred from direct assay. Source: MGI postsynaptic density Inferred from direct assay. Source: MGI synaptosome Inferred from direct assay. Source: MGI
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
ABI1	Q8IZP0	2	EBI-375596,EBI-375446	From a different organism.
Abi1	Q8CBW3	1	EBI-375596,EBI-375511
BAIAP2	Q9UQB8	6	EBI-375596,EBI-525456	From a different organism.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 821

821

Epidermal growth factor receptor kinase substrate 8

PRO_0000086995

Regions

Domain

69 – 129

PH; first part

Domain

381 – 414

PH; second part

Domain

532 – 591

SH3

Compositional bias

210 – 213

Poly-Pro

Compositional bias

322 – 325

Poly-Pro

Compositional bias

421 – 440

Pro-rich

Compositional bias

620 – 650

Pro-rich

Compositional bias

658 – 663

Poly-Ser

Amino acid modifications

Modified residue

524

Phosphotyrosine By similarity

Modified residue

810

Phosphoserine By similarity

Modified residue

814

Phosphoserine By similarity

Secondary structure

821

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

Q08509-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 6B9EB95DD22D910C
Show »

FASTA

821

91,738

        10         20         30         40         50         60 
MNGHMSNRSS GYGVYPSQLN GYGSSPPYSQ MDREHSSRTS AKALYEQRKN YARDSVSSVS 

        70         80         90        100        110        120 
DVSQYRVEHL TTFVLDRKDA MITVEDGIRK LKLLDAKGKV WTQDMILQVD DRAVSLIDLE 

       130        140        150        160        170        180 
SKNELENFPL NTISHCQAVV HACSYDSILA LVCKEPTQSK PDLHLFQCDE VKANLISEDI 

       190        200        210        220        230        240 
ESAISDSKGG KQKRRPEALR MIAKADPGIP PPPRAPAPVP PGTVTQVDVR SRVAAWSAWA 

       250        260        270        280        290        300 
ADQGDFEKPR QYHEQEETPE MMAARIDRDV QILNHILDDI EFFITKLQKA AEAFSELSKR 

       310        320        330        340        350        360 
KKSKKSKRKG PGEGVLTLRA KPPPPDEFVD CFQKFKHGFN LLAKLKSHIQ NPSASDLVHF 

       370        380        390        400        410        420 
LFTPLNMVVQ ATGGPELASS VLSPLLTKDT VDFLNYTATA EERKLWMSLG DSWVKVRAEW 

       430        440        450        460        470        480 
PKEQFIPPYV PRFRNGWEPP MLNFMGAPTE QDMYQLAESV ANAEHQRKQD SKRLSTEHSN 

       490        500        510        520        530        540 
VSDYPPADGY AYSSSMYHRG PHADHGEAAM PFKSTPNHQV DRNYDAVKTQ PKKYAKSKYD 

       550        560        570        580        590        600 
FVARNSSELS VMKDDVLEIL DDRRQWWKVR NASGDSGFVP NNILDIMRTP ESGVGRADPP 

       610        620        630        640        650        660 
YTHTIQKQRT EYGLRSADTP SAPSPPPTPA PVPVPLPPSV PAPVSVPKVP ADVTRQNSSS 

       670        680        690        700        710        720 
SDSGGSIVRD SQRYKQLPVD RRKSQMEEVQ DELFQRLTIG RSAAQRKFHV PRQNVPVINI 

       730        740        750        760        770        780 
TYDSSPEEVK TWLQSKGFNP VTVNSLGVLN GAQLFSLNKD ELRSVCPEGA RVFNQITVQK 

       790        800        810        820 
AALEDSNGSS ELQEIMRRRQ EKISAAASDS GVESFDEGSS H

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Eps8, a substrate for the epidermal growth factor receptor kinase, enhances EGF-dependent mitogenic signals." Fazioli F., Minichiello L., Matoska V., Castagnino P., Miki T., Wong W.T., di Fiore P.P. EMBO J. 12:3799-3808(1993) [PubMed: 8404850] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
[3]	"Molecular interactions of the Src homology 2 domain protein Shb with phosphotyrosine residues, tyrosine kinase receptors and Src homology 3 domain proteins." Karlsson T., Songyang Z., Landgren E., Lavergne C., Di Fiore P.P., Anafi M., Pawson T., Cantley L.C., Claesson-Welsh L., Welsh M. Oncogene 10:1475-1483(1995) [PubMed: 7537362] [Abstract] Cited for: INTERACTION WITH SHB.
[4]	"The SH3 domain of Eps8 exists as a novel intertwined dimer." Kishan K.V.R., Scita G., Wong W.T., di Fiore P.P., Newcomer M.E. Nat. Struct. Biol. 4:739-743(1997) [PubMed: 9303002] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 532-591, HOMODIMERIZATION.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

L21671 mRNA. Translation: AAA16358.1.
BC016890 mRNA. Translation: AAH16890.1.

IPI

IPI00622390.

PIR

S39983.

UniGene

Mm.235346
Mm.412332

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AOJ	X-ray	2.50	A/B	532-591	[»]
1I07	X-ray	1.80	A/B	532-591	[»]
1I0C	X-ray	2.00	A/B	532-591	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q08509. 3 interactions.

PTM databases

PhosphoSite

Q08509.

Genome annotation databases

Ensembl

ENSMUSG00000015766. Mus musculus. [Contig view]

Organism-specific databases

MGI

MGI:104684. Eps8.

Phylogenomic databases

HOVERGEN

Q08509.

Gene expression databases

ArrayExpress

Q08509.

Bgee

Q08509.

GermOnline

ENSMUSG00000015766. Mus musculus.

Family and domain databases

InterPro

IPR013625. PTB.
IPR006020. PTB_PID.
IPR001452. SH3_domain.
[Graphical view]

Pfam

PF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]

ProDom

PD000066. SH3. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00462. PTB. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]

PROSITE

PS50003. PH_DOMAIN. False negative.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

SOURCE

Search...

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Entry information

Entry name

EPS8_MOUSE

Accession

Primary (citable) accession number: Q08509

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents