Reviewed,
UniProtKB/Swiss-Prot Q08509 (EPS8_MOUSE)
Last modified
June 16, 2009.
Version 83.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Epidermal growth factor receptor kinase substrate 8 | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 821 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Upon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets. |
| Subunit structure | Homodimer. Interacts with BAIAP2 By similarity. Interacts with SHB. |
| Domain | The SH3 domain mediates interaction with SHB By similarity. |
| Post-translational modification | Phosphorylated by several receptor tyrosine kinases. |
| Sequence similarities | Belongs to the EPS8 family. Contains 1 PH domain. Contains 1 SH3 domain. |
Ontologies
| Keywords | |
|---|---|
| Domain | SH3 domain |
| PTM | Phosphoprotein |
| Technical term | 3D-structure |
| Gene Ontology (GO) | |
| Biological process | actin cytoskeleton reorganization Inferred from mutant phenotype. Source: MGI adult locomotory behaviorInferred from mutant phenotype. Source: MGI behavioral response to ethanolInferred from mutant phenotype. Source: MGI |
| Cellular component | N-methyl-D-aspartate selective glutamate receptor complex Inferred from direct assay. Source: MGI postsynaptic densityInferred from direct assay. Source: MGI synaptosomeInferred from direct assay. Source: MGI |
| Molecular function | protein binding Inferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| ABI1 | Q8IZP0 | 2 | EBI-375596,EBI-375446 | From a different organism. |
| Abi1 | Q8CBW3 | 1 | EBI-375596,EBI-375511 | |
| BAIAP2 | Q9UQB8 | 6 | EBI-375596,EBI-525456 | From a different organism. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||
Molecule processing | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 821 | 821 | Epidermal growth factor receptor kinase substrate 8 | PRO_0000086995 | |||||||||||||||||
Regions | |||||||||||||||||||||
| Domain | 69 – 129 | 61 | PH; first part | ||||||||||||||||||
| Domain | 381 – 414 | 34 | PH; second part | ||||||||||||||||||
| Domain | 532 – 591 | 60 | SH3 | ||||||||||||||||||
| Compositional bias | 210 – 213 | 4 | Poly-Pro | ||||||||||||||||||
| Compositional bias | 322 – 325 | 4 | Poly-Pro | ||||||||||||||||||
| Compositional bias | 421 – 440 | 20 | Pro-rich | ||||||||||||||||||
| Compositional bias | 620 – 650 | 31 | Pro-rich | ||||||||||||||||||
| Compositional bias | 658 – 663 | 6 | Poly-Ser | ||||||||||||||||||
Amino acid modifications | |||||||||||||||||||||
| Modified residue | 524 | 1 | Phosphotyrosine By similarity | ||||||||||||||||||
| Modified residue | 810 | 1 | Phosphoserine By similarity | ||||||||||||||||||
| Modified residue | 814 | 1 | Phosphoserine By similarity | ||||||||||||||||||
Secondary structure | |||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||
| Beta strand | 534 – 539 | 6 | |||||||||||||||||||
| Beta strand | 556 – 559 | 4 | |||||||||||||||||||
| Helix | 561 – 563 | 3 | |||||||||||||||||||
| Beta strand | 566 – 570 | 5 | |||||||||||||||||||
| Beta strand | 576 – 580 | 5 | |||||||||||||||||||
| Helix | 581 – 583 | 3 | |||||||||||||||||||
| Beta strand | 584 – 587 | 4 | |||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Eps8, a substrate for the epidermal growth factor receptor kinase, enhances EGF-dependent mitogenic signals." Fazioli F., Minichiello L., Matoska V., Castagnino P., Miki T., Wong W.T., di Fiore P.P. EMBO J. 12:3799-3808(1993) [PubMed: 8404850] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney. |
| [3] | "Molecular interactions of the Src homology 2 domain protein Shb with phosphotyrosine residues, tyrosine kinase receptors and Src homology 3 domain proteins." Karlsson T., Songyang Z., Landgren E., Lavergne C., Di Fiore P.P., Anafi M., Pawson T., Cantley L.C., Claesson-Welsh L., Welsh M. Oncogene 10:1475-1483(1995) [PubMed: 7537362] [Abstract] Cited for: INTERACTION WITH SHB. |
| [4] | "The SH3 domain of Eps8 exists as a novel intertwined dimer." Kishan K.V.R., Scita G., Wong W.T., di Fiore P.P., Newcomer M.E. Nat. Struct. Biol. 4:739-743(1997) [PubMed: 9303002] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 532-591, HOMODIMERIZATION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| L21671 mRNA. Translation: AAA16358.1. BC016890 mRNA. Translation: AAH16890.1. | |||||||||||||||||||||||||
| IPI | IPI00622390. | ||||||||||||||||||||||||
| PIR | S39983. | ||||||||||||||||||||||||
| UniGene | Mm.235346 Mm.412332 | ||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||
| IntAct | Q08509. 3 interactions. | ||||||||||||||||||||||||
PTM databases | |||||||||||||||||||||||||
| PhosphoSite | Q08509. | ||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||
| Ensembl | ENSMUSG00000015766. Mus musculus. [Contig view] | ||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||
| MGI | MGI:104684. Eps8. | ||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||
| HOVERGEN | Q08509. | ||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||
| ArrayExpress | Q08509. | ||||||||||||||||||||||||
| Bgee | Q08509. | ||||||||||||||||||||||||
| GermOnline | ENSMUSG00000015766. Mus musculus. | ||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||
| InterPro | IPR013625. PTB. IPR006020. PTB_PID. IPR001452. SH3_domain. [Graphical view] | ||||||||||||||||||||||||
| Pfam | PF08416. PTB. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view] | ||||||||||||||||||||||||
| ProDom | PD000066. SH3. 1 hit. [Graphical view] [Entries sharing at least one domain] | ||||||||||||||||||||||||
| SMART | SM00462. PTB. 1 hit. SM00326. SH3. 1 hit. [Graphical view] | ||||||||||||||||||||||||
| PROSITE | PS50003. PH_DOMAIN. False negative. PS50002. SH3. 1 hit. [Graphical view] | ||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||
Other Resources | |||||||||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||||||||
Entry information
| Entry name | EPS8_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q08509 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


