Q08509 (EPS8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Epidermal growth factor receptor kinase substrate 8

Gene names

Name:

Eps8

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	821 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Upon binding to EGF receptor/EGFR enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets. Ref.1
Subunit structure	Homodimer. Interacts with BAIAP2 By similarity. Interacts with SHB and LANCL1. Interacts with EGFR; mediates EPS8 phosphorylation. Ref.1 Ref.4 Ref.5 Ref.6
Domain	The SH3 domain mediates interaction with SHB By similarity.
Post-translational modification	Phosphorylated by several receptor tyrosine kinases. Ref.1
Sequence similarities	Belongs to the EPS8 family. Contains 1 PH domain. Contains 1 SH3 domain.

Ontologies

Keywords
Domain	SH3 domain
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	actin cytoskeleton reorganization Inferred from mutant phenotype. Source: MGI adult locomotory behavior Inferred from mutant phenotype. Source: MGI behavioral response to ethanol Inferred from mutant phenotype. Source: MGI
Cellular component	N-methyl-D-aspartate selective glutamate receptor complex Inferred from direct assay. Source: MGI postsynaptic density Inferred from direct assay. Source: MGI synaptosome Inferred from direct assay. Source: MGI
Molecular function	protein binding Inferred from physical interaction Ref.5. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ABI1	Q8IZP0	2	EBI-375596,EBI-375446	From a different organism.
Abi1	Q8CBW3	2	EBI-375596,EBI-375511
BAIAP2	Q9UQB8	8	EBI-375596,EBI-525456	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 821

821

Epidermal growth factor receptor kinase substrate 8

PRO_0000086995

Regions

Domain

69 – 129

61

PH; first part

Domain

381 – 414

34

PH; second part

Domain

532 – 591

60

SH3

Compositional bias

210 – 213

4

Poly-Pro

Compositional bias

322 – 325

4

Poly-Pro

Compositional bias

421 – 440

20

Pro-rich

Compositional bias

620 – 650

31

Pro-rich

Compositional bias

658 – 663

6

Poly-Ser

Amino acid modifications

Modified residue

484

1

Phosphotyrosine By similarity

Modified residue

524

1

Phosphotyrosine By similarity

Modified residue

658

1

Phosphoserine By similarity

Modified residue

660

1

Phosphoserine By similarity

Modified residue

661

1

Phosphoserine By similarity

Modified residue

810

1

Phosphoserine By similarity

Modified residue

814

1

Phosphoserine By similarity

Experimental info

Sequence conflict

652

1

N → D in AAA16358. Ref.1

Sequence conflict

652

1

N → D in AAH16890. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

821

Helix Strand Turn

Details...

Beta strand

534 – 539

6

Beta strand

556 – 559

4

Helix

561 – 563

3

Beta strand

566 – 570

5

Beta strand

576 – 580

5

Helix

581 – 583

3

Beta strand

584 – 587

4

Sequences

	Sequence		Length	Mass (Da)
	Q08509 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 50F691FAC3EF1304	FASTA	821	91,737
	10 20 30 40 50 60 MNGHMSNRSS GYGVYPSQLN GYGSSPPYSQ MDREHSSRTS AKALYEQRKN YARDSVSSVS 70 80 90 100 110 120 DVSQYRVEHL TTFVLDRKDA MITVEDGIRK LKLLDAKGKV WTQDMILQVD DRAVSLIDLE 130 140 150 160 170 180 SKNELENFPL NTISHCQAVV HACSYDSILA LVCKEPTQSK PDLHLFQCDE VKANLISEDI 190 200 210 220 230 240 ESAISDSKGG KQKRRPEALR MIAKADPGIP PPPRAPAPVP PGTVTQVDVR SRVAAWSAWA 250 260 270 280 290 300 ADQGDFEKPR QYHEQEETPE MMAARIDRDV QILNHILDDI EFFITKLQKA AEAFSELSKR 310 320 330 340 350 360 KKSKKSKRKG PGEGVLTLRA KPPPPDEFVD CFQKFKHGFN LLAKLKSHIQ NPSASDLVHF 370 380 390 400 410 420 LFTPLNMVVQ ATGGPELASS VLSPLLTKDT VDFLNYTATA EERKLWMSLG DSWVKVRAEW 430 440 450 460 470 480 PKEQFIPPYV PRFRNGWEPP MLNFMGAPTE QDMYQLAESV ANAEHQRKQD SKRLSTEHSN 490 500 510 520 530 540 VSDYPPADGY AYSSSMYHRG PHADHGEAAM PFKSTPNHQV DRNYDAVKTQ PKKYAKSKYD 550 560 570 580 590 600 FVARNSSELS VMKDDVLEIL DDRRQWWKVR NASGDSGFVP NNILDIMRTP ESGVGRADPP 610 620 630 640 650 660 YTHTIQKQRT EYGLRSADTP SAPSPPPTPA PVPVPLPPSV PAPVSVPKVP ANVTRQNSSS 670 680 690 700 710 720 SDSGGSIVRD SQRYKQLPVD RRKSQMEEVQ DELFQRLTIG RSAAQRKFHV PRQNVPVINI 730 740 750 760 770 780 TYDSSPEEVK TWLQSKGFNP VTVNSLGVLN GAQLFSLNKD ELRSVCPEGA RVFNQITVQK 790 800 810 820 AALEDSNGSS ELQEIMRRRQ EKISAAASDS GVESFDEGSS H

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Eps8, a substrate for the epidermal growth factor receptor kinase, enhances EGF-dependent mitogenic signals." Fazioli F., Minichiello L., Matoska V., Castagnino P., Miki T., Wong W.T., di Fiore P.P. EMBO J. 12:3799-3808(1993) [PubMed: 8404850] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN CELL GROWTH, INTERACTION WITH EGFR, PHOSPHORYLATION.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Bone marrow and Lung.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
[4]	"Molecular interactions of the Src homology 2 domain protein Shb with phosphotyrosine residues, tyrosine kinase receptors and Src homology 3 domain proteins." Karlsson T., Songyang Z., Landgren E., Lavergne C., Di Fiore P.P., Anafi M., Pawson T., Cantley L.C., Claesson-Welsh L., Welsh M. Oncogene 10:1475-1483(1995) [PubMed: 7537362] [Abstract] Cited for: INTERACTION WITH SHB.
[5]	"Structure of human lanthionine synthetase C-like protein 1 and its interaction with Eps8 and glutathione." Zhang W., Wang L., Liu Y., Xu J., Zhu G., Cang H., Li X., Bartlam M., Hensley K., Li G., Rao Z., Zhang X.C. Genes Dev. 23:1387-1392(2009) [PubMed: 19528316] [Abstract] Cited for: INTERACTION WITH LANCL1.
[6]	"The SH3 domain of Eps8 exists as a novel intertwined dimer." Kishan K.V.R., Scita G., Wong W.T., di Fiore P.P., Newcomer M.E. Nat. Struct. Biol. 4:739-743(1997) [PubMed: 9303002] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 532-591, HOMODIMERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L21671 mRNA. Translation: AAA16358.1.
AK149683 mRNA. Translation: BAE29023.1.
AK166156 mRNA. Translation: BAE38601.1.
BC016890 mRNA. Translation: AAH16890.1.

IPI

IPI00622390.

PIR

S39983.

RefSeq

NP_031971.2. NM_007945.2.

UniGene

Mm.235346.
Mm.412332.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AOJ	X-ray	2.50	A/B	532-591	[»]
1I07	X-ray	1.80	A/B	532-591	[»]
1I0C	X-ray	2.00	A/B	532-591	[»]

ProteinModelPortal

Q08509.

SMR

Q08509. Positions 59-188, 532-590, 698-783.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-32858N.

IntAct

Q08509. 3 interactions.

STRING

Q08509.

PTM databases

PhosphoSite

Q08509.

Proteomic databases

PRIDE

Q08509.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000058210; ENSMUSP00000052776; ENSMUSG00000015766.
ENSMUST00000100841; ENSMUSP00000098402; ENSMUSG00000015766.
ENSMUST00000111878; ENSMUSP00000107509; ENSMUSG00000015766.

GeneID

13860.

KEGG

mmu:13860.

Organism-specific databases

CTD

2059.

MGI

MGI:104684. Eps8.

Phylogenomic databases

eggNOG

roNOG10217.

HOVERGEN

HBG003090.

InParanoid

Q08509.

OrthoDB

EOG42V8FP.

Gene expression databases

ArrayExpress

Q08509.

Bgee

Q08509.

Genevestigator

Q08509.

GermOnline

ENSMUSG00000015766. Mus musculus.

Family and domain databases

InterPro

IPR011993. PH_type.
IPR013625. PTB.
IPR006020. PTyr_interaction_dom.
IPR001452. SH3_domain.
[Graphical view]

Gene3D

G3DSA:2.30.29.30. PH_type. 1 hit.

Pfam

PF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]

SMART

SM00462. PTB. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]

SUPFAM

SSF50044. SH3. 1 hit.

PROSITE

PS50003. PH_DOMAIN. False negative.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

SOURCE

Search...

Entry information

Entry name

EPS8_MOUSE

Accession

Primary (citable) accession number: Q08509
Secondary accession number(s): Q3TM41

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 27, 2011
Last modified:	December 14, 2011
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program