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Protein

Prolactin receptor

Gene

Prlr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is a receptor for the anterior pituitary hormone prolactin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi206ZincBy similarity1
Metal bindingi207ZincBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • peptide hormone binding Source: MGI
  • prolactin receptor activity Source: MGI

GO - Biological processi

  • activation of transmembrane receptor protein tyrosine kinase activity Source: MGI
  • JAK-STAT cascade Source: MGI
  • lactation Source: MGI
  • mammary gland alveolus development Source: MGI
  • mammary gland epithelial cell differentiation Source: MGI
  • mammary gland epithelium development Source: MGI
  • prostate gland growth Source: MGI
  • regulation of cell adhesion Source: MGI
  • regulation of epithelial cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1170546. Prolactin receptor signaling.
R-MMU-982772. Growth hormone receptor signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolactin receptor
Short name:
PRL-R
Gene namesi
Name:Prlr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:97763. Prlr.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 229ExtracellularBy similarityAdd BLAST210
Transmembranei230 – 253HelicalBy similarityAdd BLAST24
Topological domaini254 – 608CytoplasmicBy similarityAdd BLAST355

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

GuidetoPHARMACOLOGYi1721.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000001097820 – 608Prolactin receptorAdd BLAST589

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 41By similarity
Glycosylationi54N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi70 ↔ 81By similarity
Glycosylationi99N-linked (GlcNAc...)Sequence analysis1
Glycosylationi127N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ08501.
PRIDEiQ08501.

PTM databases

iPTMnetiQ08501.
PhosphoSitePlusiQ08501.

Expressioni

Gene expression databases

BgeeiENSMUSG00000005268.
CleanExiMM_PRLR.
ExpressionAtlasiQ08501. baseline and differential.
GenevisibleiQ08501. MM.

Interactioni

Subunit structurei

Interacts with SMARCA1. Interacts with NEK3 and VAV2 and this interaction is prolactin-dependent.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
AGAP2Q99490-22EBI-7737664,EBI-7737644From a different organism.

Protein-protein interaction databases

BioGridi202385. 3 interactors.
IntActiQ08501. 3 interactors.
MINTiMINT-4596793.
STRINGi10090.ENSMUSP00000122219.

Structurei

3D structure databases

ProteinModelPortaliQ08501.
SMRiQ08501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 122Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST101
Domaini124 – 224Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST101

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi210 – 214WSXWS motif5
Motifi262 – 270Box 1 motif9

Domaini

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.

Sequence similaritiesi

Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IUSW. Eukaryota.
ENOG410XRJS. LUCA.
GeneTreeiENSGT00530000063112.
HOGENOMiHOG000059569.
HOVERGENiHBG007314.
InParanoidiQ08501.
KOiK05081.
OMAiSGPNSCY.
OrthoDBiEOG091G07CK.
PhylomeDBiQ08501.
TreeFamiTF330851.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR015152. Growth/epo_recpt_lig-bind.
IPR013783. Ig-like_fold.
IPR003528. Long_hematopoietin_rcpt_CS.
IPR033230. PRLR.
[Graphical view]
PANTHERiPTHR23036:SF86. PTHR23036:SF86. 1 hit.
PfamiPF09067. EpoR_lig-bind. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 2 hits.
PS01352. HEMATOPO_REC_L_F1. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform PRL-R3 (identifier: Q08501-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSALAYMLL VLSISLLNGQ SPPGKPEIHK CRSPDKETFT CWWNPGSDGG
60 70 80 90 100
LPTNYSLTYS KEGEKNTYEC PDYKTSGPNS CFFSKQYTSI WKIYIITVNA
110 120 130 140 150
TNEMGSSTSD PLYVDVTYIV EPEPPRNLTL EVKQLKDKKT YLWVKWLPPT
160 170 180 190 200
ITDVKTGWFT MEYEIRLKSE EADEWEIHFT GHQTQFKVFD LYPGQKYLVQ
210 220 230 240 250
TRCKPDHGYW SRWGQEKSIE IPNDFTLKDT TVWIIVAVLS AVICLIMVWA
260 270 280 290 300
VALKGYSMMT CIFPPVPGPK IKGFDTHLLE KGKSEELLSA LGCQDFPPTS
310 320 330 340 350
DCEDLLVEFL EVDDNEDERL MPSHSKEYPG QGVKPTHLDP DSDSGHGSYD
360 370 380 390 400
SHSLLSEKCE EPQAYPPAFH IPEITEKPEN PEANIPPTPN PQNNTPNCHT
410 420 430 440 450
DTSKSTTWPL PPGQHTRRSP YHSIADVCKL AGSPGDTLDS FLDKAEENVL
460 470 480 490 500
KLSEDAGEEE VAVQEGAKSF PSDKQNTSWP PLQEKGPIVY AKPPDYVEIH
510 520 530 540 550
KVNKDGVLSL LPKQRENHQT ENPGVPETSK EYAKVSGVTD NNILVLVPDS
560 570 580 590 600
RAQNTALLEE SAKKVPPSLE QNQSEKDLAS FTATSSNCRL QLGRLDYLDP

TCFMHSFH
Length:608
Mass (Da):68,241
Last modified:February 1, 1995 - v1
Checksum:iB8CE202B2EFC9FC6
GO
Isoform PRL-R1 (identifier: Q08501-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     281-303: KGKSEELLSALGCQDFPPTSDCE → LWCSILQLTSLVKIPTTEFLCDL
     304-608: Missing.

Show »
Length:303
Mass (Da):34,734
Checksum:iE22B50EFDB77B755
GO
Isoform PRL-R2 (identifier: Q08501-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     281-292: KGKSEELLSALG → VHNKEQLENYVY
     293-608: Missing.

Show »
Length:292
Mass (Da):33,633
Checksum:iF02BA23FE6AC74F3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti558L → F in CAA51789 (PubMed:8319571).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001723281 – 303KGKSE…TSDCE → LWCSILQLTSLVKIPTTEFL CDL in isoform PRL-R1. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_001721281 – 292KGKSE…LSALG → VHNKEQLENYVY in isoform PRL-R2. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_001722293 – 608Missing in isoform PRL-R2. 1 PublicationAdd BLAST316
Alternative sequenceiVSP_001724304 – 608Missing in isoform PRL-R1. 1 PublicationAdd BLAST305

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13593 mRNA. Translation: AAC37641.1.
L14811 mRNA. Translation: AAA02686.1.
D10214 mRNA. Translation: BAA01066.1.
X73372 mRNA. Translation: CAA51789.1.
M22959 mRNA. Translation: AAA39977.1.
M22958 mRNA. Translation: AAA39976.1.
CCDSiCCDS27377.1. [Q08501-1]
CCDS56975.1. [Q08501-2]
PIRiI53269.
I77524.
I77525.
RefSeqiNP_001240711.1. NM_001253782.2. [Q08501-2]
NP_035299.4. NM_011169.5. [Q08501-1]
XP_006520097.1. XM_006520034.3. [Q08501-1]
XP_006520098.1. XM_006520035.1. [Q08501-1]
XP_006520099.1. XM_006520036.1. [Q08501-1]
XP_006520100.1. XM_006520037.2. [Q08501-1]
XP_006520101.1. XM_006520038.1. [Q08501-2]
UniGeneiMm.10516.
Mm.442446.

Genome annotation databases

EnsembliENSMUST00000124470; ENSMUSP00000122219; ENSMUSG00000005268. [Q08501-1]
ENSMUST00000128450; ENSMUSP00000122209; ENSMUSG00000005268. [Q08501-3]
ENSMUST00000137867; ENSMUSP00000121935; ENSMUSG00000005268. [Q08501-3]
ENSMUST00000148257; ENSMUSP00000118355; ENSMUSG00000005268. [Q08501-2]
GeneIDi19116.
KEGGimmu:19116.
UCSCiuc007vfw.2. mouse. [Q08501-3]
uc007vfy.2. mouse. [Q08501-1]
uc029snt.1. mouse. [Q08501-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13593 mRNA. Translation: AAC37641.1.
L14811 mRNA. Translation: AAA02686.1.
D10214 mRNA. Translation: BAA01066.1.
X73372 mRNA. Translation: CAA51789.1.
M22959 mRNA. Translation: AAA39977.1.
M22958 mRNA. Translation: AAA39976.1.
CCDSiCCDS27377.1. [Q08501-1]
CCDS56975.1. [Q08501-2]
PIRiI53269.
I77524.
I77525.
RefSeqiNP_001240711.1. NM_001253782.2. [Q08501-2]
NP_035299.4. NM_011169.5. [Q08501-1]
XP_006520097.1. XM_006520034.3. [Q08501-1]
XP_006520098.1. XM_006520035.1. [Q08501-1]
XP_006520099.1. XM_006520036.1. [Q08501-1]
XP_006520100.1. XM_006520037.2. [Q08501-1]
XP_006520101.1. XM_006520038.1. [Q08501-2]
UniGeneiMm.10516.
Mm.442446.

3D structure databases

ProteinModelPortaliQ08501.
SMRiQ08501.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202385. 3 interactors.
IntActiQ08501. 3 interactors.
MINTiMINT-4596793.
STRINGi10090.ENSMUSP00000122219.

Chemistry databases

GuidetoPHARMACOLOGYi1721.

PTM databases

iPTMnetiQ08501.
PhosphoSitePlusiQ08501.

Proteomic databases

PaxDbiQ08501.
PRIDEiQ08501.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000124470; ENSMUSP00000122219; ENSMUSG00000005268. [Q08501-1]
ENSMUST00000128450; ENSMUSP00000122209; ENSMUSG00000005268. [Q08501-3]
ENSMUST00000137867; ENSMUSP00000121935; ENSMUSG00000005268. [Q08501-3]
ENSMUST00000148257; ENSMUSP00000118355; ENSMUSG00000005268. [Q08501-2]
GeneIDi19116.
KEGGimmu:19116.
UCSCiuc007vfw.2. mouse. [Q08501-3]
uc007vfy.2. mouse. [Q08501-1]
uc029snt.1. mouse. [Q08501-2]

Organism-specific databases

CTDi5618.
MGIiMGI:97763. Prlr.

Phylogenomic databases

eggNOGiENOG410IUSW. Eukaryota.
ENOG410XRJS. LUCA.
GeneTreeiENSGT00530000063112.
HOGENOMiHOG000059569.
HOVERGENiHBG007314.
InParanoidiQ08501.
KOiK05081.
OMAiSGPNSCY.
OrthoDBiEOG091G07CK.
PhylomeDBiQ08501.
TreeFamiTF330851.

Enzyme and pathway databases

ReactomeiR-MMU-1170546. Prolactin receptor signaling.
R-MMU-982772. Growth hormone receptor signaling.

Miscellaneous databases

ChiTaRSiPrlr. mouse.
PROiQ08501.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005268.
CleanExiMM_PRLR.
ExpressionAtlasiQ08501. baseline and differential.
GenevisibleiQ08501. MM.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR015152. Growth/epo_recpt_lig-bind.
IPR013783. Ig-like_fold.
IPR003528. Long_hematopoietin_rcpt_CS.
IPR033230. PRLR.
[Graphical view]
PANTHERiPTHR23036:SF86. PTHR23036:SF86. 1 hit.
PfamiPF09067. EpoR_lig-bind. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 2 hits.
PS01352. HEMATOPO_REC_L_F1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRLR_MOUSE
AccessioniPrimary (citable) accession number: Q08501
Secondary accession number(s): P15212, P15213, Q62099
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.