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Protein

Prolactin receptor

Gene

Prlr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is a receptor for the anterior pituitary hormone prolactin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi206 – 2061ZincBy similarity
Metal bindingi207 – 2071ZincBy similarity

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • peptide hormone binding Source: MGI
  • prolactin receptor activity Source: MGI

GO - Biological processi

  • activation of transmembrane receptor protein tyrosine kinase activity Source: MGI
  • JAK-STAT cascade Source: MGI
  • lactation Source: MGI
  • mammary gland alveolus development Source: MGI
  • mammary gland epithelial cell differentiation Source: MGI
  • mammary gland epithelium development Source: MGI
  • prolactin signaling pathway Source: GOC
  • prostate gland growth Source: MGI
  • regulation of cell adhesion Source: MGI
  • regulation of epithelial cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1170546. Prolactin receptor signaling.
R-MMU-982772. Growth hormone receptor signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolactin receptor
Short name:
PRL-R
Gene namesi
Name:Prlr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:97763. Prlr.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 229210ExtracellularBy similarityAdd
BLAST
Transmembranei230 – 25324HelicalBy similarityAdd
BLAST
Topological domaini254 – 608355CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • cell surface Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry

GuidetoPHARMACOLOGYi1721.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 608589Prolactin receptorPRO_0000010978Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 41By similarity
Glycosylationi54 – 541N-linked (GlcNAc...)Sequence analysis
Disulfide bondi70 ↔ 81By similarity
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence analysis
Glycosylationi127 – 1271N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ08501.
PRIDEiQ08501.

PTM databases

iPTMnetiQ08501.
PhosphoSiteiQ08501.

Expressioni

Gene expression databases

BgeeiQ08501.
CleanExiMM_PRLR.
ExpressionAtlasiQ08501. baseline and differential.
GenevisibleiQ08501. MM.

Interactioni

Subunit structurei

Interacts with SMARCA1. Interacts with NEK3 and VAV2 and this interaction is prolactin-dependent.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
AGAP2Q99490-22EBI-7737664,EBI-7737644From a different organism.

Protein-protein interaction databases

BioGridi202385. 3 interactions.
IntActiQ08501. 3 interactions.
MINTiMINT-4596793.
STRINGi10090.ENSMUSP00000122219.

Structurei

3D structure databases

ProteinModelPortaliQ08501.
SMRiQ08501. Positions 20-221.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 122101Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini124 – 224101Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi210 – 2145WSXWS motif
Motifi262 – 2709Box 1 motif

Domaini

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.

Sequence similaritiesi

Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IUSW. Eukaryota.
ENOG410XRJS. LUCA.
GeneTreeiENSGT00530000063112.
HOGENOMiHOG000059569.
HOVERGENiHBG007314.
InParanoidiQ08501.
KOiK05081.
OMAiSGPNSCY.
OrthoDBiEOG7Q5HCH.
PhylomeDBiQ08501.
TreeFamiTF330851.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR015152. Growth/epo_recpt_lig-bind.
IPR013783. Ig-like_fold.
IPR003528. Long_hematopoietin_rcpt_CS.
IPR033230. PRLR.
[Graphical view]
PANTHERiPTHR23036:SF86. PTHR23036:SF86. 1 hit.
PfamiPF09067. EpoR_lig-bind. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 2 hits.
PS01352. HEMATOPO_REC_L_F1. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform PRL-R3 (identifier: Q08501-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSALAYMLL VLSISLLNGQ SPPGKPEIHK CRSPDKETFT CWWNPGSDGG
60 70 80 90 100
LPTNYSLTYS KEGEKNTYEC PDYKTSGPNS CFFSKQYTSI WKIYIITVNA
110 120 130 140 150
TNEMGSSTSD PLYVDVTYIV EPEPPRNLTL EVKQLKDKKT YLWVKWLPPT
160 170 180 190 200
ITDVKTGWFT MEYEIRLKSE EADEWEIHFT GHQTQFKVFD LYPGQKYLVQ
210 220 230 240 250
TRCKPDHGYW SRWGQEKSIE IPNDFTLKDT TVWIIVAVLS AVICLIMVWA
260 270 280 290 300
VALKGYSMMT CIFPPVPGPK IKGFDTHLLE KGKSEELLSA LGCQDFPPTS
310 320 330 340 350
DCEDLLVEFL EVDDNEDERL MPSHSKEYPG QGVKPTHLDP DSDSGHGSYD
360 370 380 390 400
SHSLLSEKCE EPQAYPPAFH IPEITEKPEN PEANIPPTPN PQNNTPNCHT
410 420 430 440 450
DTSKSTTWPL PPGQHTRRSP YHSIADVCKL AGSPGDTLDS FLDKAEENVL
460 470 480 490 500
KLSEDAGEEE VAVQEGAKSF PSDKQNTSWP PLQEKGPIVY AKPPDYVEIH
510 520 530 540 550
KVNKDGVLSL LPKQRENHQT ENPGVPETSK EYAKVSGVTD NNILVLVPDS
560 570 580 590 600
RAQNTALLEE SAKKVPPSLE QNQSEKDLAS FTATSSNCRL QLGRLDYLDP

TCFMHSFH
Length:608
Mass (Da):68,241
Last modified:February 1, 1995 - v1
Checksum:iB8CE202B2EFC9FC6
GO
Isoform PRL-R1 (identifier: Q08501-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     281-303: KGKSEELLSALGCQDFPPTSDCE → LWCSILQLTSLVKIPTTEFLCDL
     304-608: Missing.

Show »
Length:303
Mass (Da):34,734
Checksum:iE22B50EFDB77B755
GO
Isoform PRL-R2 (identifier: Q08501-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     281-292: KGKSEELLSALG → VHNKEQLENYVY
     293-608: Missing.

Show »
Length:292
Mass (Da):33,633
Checksum:iF02BA23FE6AC74F3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti558 – 5581L → F in CAA51789 (PubMed:8319571).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei281 – 30323KGKSE…TSDCE → LWCSILQLTSLVKIPTTEFL CDL in isoform PRL-R1. 1 PublicationVSP_001723Add
BLAST
Alternative sequencei281 – 29212KGKSE…LSALG → VHNKEQLENYVY in isoform PRL-R2. 1 PublicationVSP_001721Add
BLAST
Alternative sequencei293 – 608316Missing in isoform PRL-R2. 1 PublicationVSP_001722Add
BLAST
Alternative sequencei304 – 608305Missing in isoform PRL-R1. 1 PublicationVSP_001724Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13593 mRNA. Translation: AAC37641.1.
L14811 mRNA. Translation: AAA02686.1.
D10214 mRNA. Translation: BAA01066.1.
X73372 mRNA. Translation: CAA51789.1.
M22959 mRNA. Translation: AAA39977.1.
M22958 mRNA. Translation: AAA39976.1.
CCDSiCCDS27377.1. [Q08501-1]
CCDS56975.1. [Q08501-2]
PIRiI53269.
I77524.
I77525.
RefSeqiNP_001240711.1. NM_001253782.2. [Q08501-2]
NP_035299.4. NM_011169.5. [Q08501-1]
XP_006520097.1. XM_006520034.2. [Q08501-1]
XP_006520098.1. XM_006520035.1. [Q08501-1]
XP_006520099.1. XM_006520036.1. [Q08501-1]
XP_006520100.1. XM_006520037.1. [Q08501-1]
XP_006520101.1. XM_006520038.1. [Q08501-2]
UniGeneiMm.10516.
Mm.442446.

Genome annotation databases

EnsembliENSMUST00000124470; ENSMUSP00000122219; ENSMUSG00000005268. [Q08501-1]
ENSMUST00000128450; ENSMUSP00000122209; ENSMUSG00000005268. [Q08501-3]
ENSMUST00000137867; ENSMUSP00000121935; ENSMUSG00000005268. [Q08501-3]
ENSMUST00000148257; ENSMUSP00000118355; ENSMUSG00000005268. [Q08501-2]
GeneIDi19116.
KEGGimmu:19116.
UCSCiuc007vfw.2. mouse. [Q08501-3]
uc007vfy.2. mouse. [Q08501-1]
uc029snt.1. mouse. [Q08501-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13593 mRNA. Translation: AAC37641.1.
L14811 mRNA. Translation: AAA02686.1.
D10214 mRNA. Translation: BAA01066.1.
X73372 mRNA. Translation: CAA51789.1.
M22959 mRNA. Translation: AAA39977.1.
M22958 mRNA. Translation: AAA39976.1.
CCDSiCCDS27377.1. [Q08501-1]
CCDS56975.1. [Q08501-2]
PIRiI53269.
I77524.
I77525.
RefSeqiNP_001240711.1. NM_001253782.2. [Q08501-2]
NP_035299.4. NM_011169.5. [Q08501-1]
XP_006520097.1. XM_006520034.2. [Q08501-1]
XP_006520098.1. XM_006520035.1. [Q08501-1]
XP_006520099.1. XM_006520036.1. [Q08501-1]
XP_006520100.1. XM_006520037.1. [Q08501-1]
XP_006520101.1. XM_006520038.1. [Q08501-2]
UniGeneiMm.10516.
Mm.442446.

3D structure databases

ProteinModelPortaliQ08501.
SMRiQ08501. Positions 20-221.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202385. 3 interactions.
IntActiQ08501. 3 interactions.
MINTiMINT-4596793.
STRINGi10090.ENSMUSP00000122219.

Chemistry

GuidetoPHARMACOLOGYi1721.

PTM databases

iPTMnetiQ08501.
PhosphoSiteiQ08501.

Proteomic databases

PaxDbiQ08501.
PRIDEiQ08501.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000124470; ENSMUSP00000122219; ENSMUSG00000005268. [Q08501-1]
ENSMUST00000128450; ENSMUSP00000122209; ENSMUSG00000005268. [Q08501-3]
ENSMUST00000137867; ENSMUSP00000121935; ENSMUSG00000005268. [Q08501-3]
ENSMUST00000148257; ENSMUSP00000118355; ENSMUSG00000005268. [Q08501-2]
GeneIDi19116.
KEGGimmu:19116.
UCSCiuc007vfw.2. mouse. [Q08501-3]
uc007vfy.2. mouse. [Q08501-1]
uc029snt.1. mouse. [Q08501-2]

Organism-specific databases

CTDi5618.
MGIiMGI:97763. Prlr.

Phylogenomic databases

eggNOGiENOG410IUSW. Eukaryota.
ENOG410XRJS. LUCA.
GeneTreeiENSGT00530000063112.
HOGENOMiHOG000059569.
HOVERGENiHBG007314.
InParanoidiQ08501.
KOiK05081.
OMAiSGPNSCY.
OrthoDBiEOG7Q5HCH.
PhylomeDBiQ08501.
TreeFamiTF330851.

Enzyme and pathway databases

ReactomeiR-MMU-1170546. Prolactin receptor signaling.
R-MMU-982772. Growth hormone receptor signaling.

Miscellaneous databases

ChiTaRSiPrlr. mouse.
PROiQ08501.
SOURCEiSearch...

Gene expression databases

BgeeiQ08501.
CleanExiMM_PRLR.
ExpressionAtlasiQ08501. baseline and differential.
GenevisibleiQ08501. MM.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR015152. Growth/epo_recpt_lig-bind.
IPR013783. Ig-like_fold.
IPR003528. Long_hematopoietin_rcpt_CS.
IPR033230. PRLR.
[Graphical view]
PANTHERiPTHR23036:SF86. PTHR23036:SF86. 1 hit.
PfamiPF09067. EpoR_lig-bind. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 2 hits.
PS01352. HEMATOPO_REC_L_F1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequencing of the cDNA encoding the murine mammary gland long-form prolactin receptor."
    Moore R.C., Oka T.
    Gene 134:263-265(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PRL-R3).
    Strain: C3H/HeJ.
    Tissue: Mammary gland.
  2. "Changes in prolactin receptor expression during pregnancy in the mouse ovary."
    Clarke D.L., Linzer D.I.H.
    Endocrinology 133:224-232(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PRL-R3).
    Strain: Swiss Webster.
    Tissue: Liver.
  3. Sasaki M.
    Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PRL-R3).
  4. Edery M., Pezet A., Nandi S., Kelly P.A.
    Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PRL-R3).
    Strain: BALB/cJ.
    Tissue: Mammary gland.
  5. "Expression of multiple forms of the prolactin receptor in mouse liver."
    Davis J.A., Linzer D.I.H.
    Mol. Endocrinol. 3:674-680(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS PRL-R2 AND PRL-R1).
    Strain: Swiss Webster.
    Tissue: Liver.

Entry informationi

Entry nameiPRLR_MOUSE
AccessioniPrimary (citable) accession number: Q08501
Secondary accession number(s): P15212, P15213, Q62099
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.