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Q084I8

- BIOB_SHEFN

UniProt

Q084I8 - BIOB_SHEFN

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Protein

Biotin synthase

Gene
bioB, Sfri_1475
Organism
Shewanella frigidimarina (strain NCIMB 400)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi100 – 1001Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi131 – 1311Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi191 – 1911Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi263 – 2631Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSFRI318167:GIXS-1534-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Sfri_1475
OrganismiShewanella frigidimarina (strain NCIMB 400)
Taxonomic identifieri318167 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000000684: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350Biotin synthaseUniRule annotationPRO_0000381618Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi318167.Sfri_1475.

Structurei

3D structure databases

ProteinModelPortaliQ084I8.
SMRiQ084I8. Positions 7-317.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q084I8-1 [UniParc]FASTAAdd to Basket

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MSLAEVRHDW QKAQIEALFA LPMNDLLFKA HSIHRETFDP NEVQISRLLS    50
IKTGACPEDC KYCPQSARYD TGLEKERLLE IDKVLTEAKS AKAAGASRFC 100
MGAAWRNPRD KDMPYLTQMV KDVRALGMET CMTLGMLSSE QAGKLADAGL 150
DYYNHNLDTS PEYYGDVITT RTYQSRLDTL TNVRASGMKV CSGGIVGMGE 200
KATDRAGLLQ QLANLEQHPD SVPINMLVKV AGTPFENIDD LDPLEFVRTI 250
AVARILMPKS RVRLSAGRES MSDELQSMCF FAGANSIFYG CKLLTTPNPE 300
ENDDMSLFRR LGLHPEQGPQ ANIENDSALL AKASAKQDKS TKQFFDAAAL 350
Length:350
Mass (Da):38,745
Last modified:October 31, 2006 - v1
Checksum:i5ECC79B2CAF0BD5A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000447 Genomic DNA. Translation: ABI71327.1.
RefSeqiWP_011636948.1. NC_008345.1.
YP_750165.1. NC_008345.1.

Genome annotation databases

EnsemblBacteriaiABI71327; ABI71327; Sfri_1475.
GeneIDi4277571.
KEGGisfr:Sfri_1475.
PATRICi23496875. VBISheFri14343_1530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000447 Genomic DNA. Translation: ABI71327.1 .
RefSeqi WP_011636948.1. NC_008345.1.
YP_750165.1. NC_008345.1.

3D structure databases

ProteinModelPortali Q084I8.
SMRi Q084I8. Positions 7-317.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 318167.Sfri_1475.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABI71327 ; ABI71327 ; Sfri_1475 .
GeneIDi 4277571.
KEGGi sfr:Sfri_1475.
PATRICi 23496875. VBISheFri14343_1530.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci SFRI318167:GIXS-1534-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCIMB 400.

Entry informationi

Entry nameiBIOB_SHEFN
AccessioniPrimary (citable) accession number: Q084I8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 31, 2006
Last modified: September 3, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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