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Protein

Protein DIA2

Gene

DIA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

F-box protein component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins (By similarity). The SCF(DIA2) complex is specifically involved in the pheromone induced degradation of phosphorylated TEC1. The SCF(DIA2) complex binds to DNA replication origins. Involved in DNA replication, genome stability, and the control of cell cycle, probably through its association to replication origins to facilitate the ubiquitination of another origin-binding protein. Required for invasive growth and growth under alkaline conditions.By similarity7 Publications

GO - Molecular functioni

  • DNA replication origin binding Source: SGD

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cellular protein complex disassembly Source: SGD
  • chromatin silencing at silent mating-type cassette Source: SGD
  • chromatin silencing at telomere Source: SGD
  • invasive growth in response to glucose limitation Source: SGD
  • protein ubiquitination Source: SGD
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: SGD
  • regulation of DNA replication Source: SGD
  • SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD

Keywordsi

Biological processCell cycle, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-33617-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein DIA2
Alternative name(s):
Digs into agar protein 2
Gene namesi
Name:DIA2
Ordered Locus Names:YOR080W
ORF Names:YOR29-31
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR080W.
SGDiS000005606. DIA2.

Subcellular locationi

GO - Cellular componenti

  • chromosome, telomeric region Source: GOC
  • nucleus Source: SGD
  • SCF ubiquitin ligase complex Source: SGD

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002330031 – 732Protein DIA2Add BLAST732

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei393PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ08496.

PTM databases

iPTMnetiQ08496.

Interactioni

Subunit structurei

Component of the SCF(DIA2) complex containing CDC53, SKP1, RBX1 and DIA2. Interacts with SKP1.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34479. 645 interactors.
DIPiDIP-6496N.
IntActiQ08496. 5 interactors.
MINTiMINT-672797.
STRINGi4932.YOR080W.

Structurei

3D structure databases

ProteinModelPortaliQ08496.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati15 – 48TPR 1Add BLAST34
Repeati78 – 111TPR 2Add BLAST34
Repeati113 – 145TPR 3Add BLAST33
Domaini204 – 251F-boxPROSITE-ProRule annotationAdd BLAST48
Repeati425 – 449LRR 1Add BLAST25
Repeati480 – 505LRR 2Add BLAST26
Repeati509 – 532LRR 3Add BLAST24
Repeati550 – 574LRR 4Add BLAST25
Repeati579 – 602LRR 5Add BLAST24
Repeati616 – 637LRR 6Add BLAST22
Repeati645 – 669LRR 7Add BLAST25

Sequence similaritiesi

Belongs to the DIA2 family.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, TPR repeat

Phylogenomic databases

HOGENOMiHOG000142330.
InParanoidiQ08496.
KOiK15068.
OMAiISCKGYL.
OrthoDBiEOG092C3LA4.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
3.80.10.10. 2 hits.
InterProiView protein in InterPro
IPR001810. F-box_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR006553. Leu-rich_rpt_Cys-con_subtyp.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
PfamiView protein in Pfam
PF00646. F-box. 1 hit.
SMARTiView protein in SMART
SM00256. FBOX. 1 hit.
SM00367. LRR_CC. 3 hits.
SUPFAMiSSF48452. SSF48452. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiView protein in PROSITE
PS50181. FBOX. 1 hit.
PS51450. LRR. 4 hits.
PS50293. TPR_REGION. 2 hits.

Sequencei

Sequence statusi: Complete.

Q08496-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPGNSGVA IDSTVLKAIE LGTRLFKSGE YLQAKRIFTN ALRVCDSYSQ
60 70 80 90 100
EQIMRIRNAY QLDTARPDNK RLYHPRYIKI LDNICACYEK LNDLKSCLDV
110 120 130 140 150
SQRLLKLEPG NIKCYIRCTR TLIKLKDWKR AYKTCSRGLQ LCNNDSNHLR
160 170 180 190 200
QQKQFIKNNM VQKQDGKRSY IDPLEETKIA KKKKNNNVLE SLPKKKIKGS
210 220 230 240 250
TKKTDLVGNL PIEILPIIFQ RFTTKELVTL SLVCNKWRDK ILYHLDCFQE
260 270 280 290 300
FNLAPINFKN FVKFMDFLQQ NFTRTYRKYI LSQVKVSSRI TSEELRITQL
310 320 330 340 350
LFSKMPKCIN IERLILSMPT LTTTQIFKLM VRGGTDFFTR LLELSLMITY
360 370 380 390 400
RPDKQHELEI LQTCPLLKKI ELIFVNSLVP IFDGNNSVGR DGSFNVMARH
410 420 430 440 450
TNMQISTADN DEQGIVEEKV IYSELEKITL ICDKKKIKNF PLCRALLRGQ
460 470 480 490 500
FPLLQKLTIT GVTFPMNNQD IMNFQWLLNF PDLKELWIED NDNCELSKFL
510 520 530 540 550
QLLKFSNVWK NLEKLTFREN KLYPIVNLDE DQPVTNDDEV PSMLFYKENL
560 570 580 590 600
QNLEKLDLMG TSISGSALTR LCEQEYLDGR KLRSLNIGNC PNIQFPNNHA
610 620 630 640 650
HTARMILDVN AVLKRLSKLE EINLSHLSSL NDSTMKSFII NVPFLENLKR
660 670 680 690 700
LDISHNFEIT GISIYEFLKK FQMDHDNEAG GQPLAYLNID GCSQVSHITV
710 720 730
NMIRAQNLVT QVDCVYERDV WRKFGINSYS YS
Length:732
Mass (Da):85,070
Last modified:October 5, 2010 - v2
Checksum:i98B610994BA2F803
GO

Sequence cautioni

The sequence CAA94565 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA99273 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA99275 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70678 Genomic DNA. Translation: CAA94565.1. Different initiation.
Z74988 Genomic DNA. Translation: CAA99273.1. Different initiation.
Z74989 Genomic DNA. Translation: CAA99275.1. Different initiation.
BK006948 Genomic DNA. Translation: DAA10859.2.
PIRiS66963.
RefSeqiNP_014723.2. NM_001183499.1.

Genome annotation databases

EnsemblFungiiYOR080W; YOR080W; YOR080W.
GeneIDi854247.
KEGGisce:YOR080W.

Similar proteinsi

Entry informationi

Entry nameiDIA2_YEAST
AccessioniPrimary (citable) accession number: Q08496
Secondary accession number(s): D6W2E3, O00034, Q7LGN4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: October 5, 2010
Last modified: August 30, 2017
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names