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Reviewed, UniProtKB/Swiss-Prot Q08493 (PDE4C_HUMAN)

Last modified November 3, 2009. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    cAMP-specific 3',5'-cyclic phosphodiesterase 4C
    EC=3.1.4.17
Alternative name(s):
    DPDE1
    PDE21
Gene names
Name: PDE4C
Synonyms: DPDE1
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length712 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Enzyme regulation

Inhibited by rolipram.

Pathway

Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.

Tissue specificity

Expressed in various tissues but not in cells of the immune system.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
Polymorphism
   LigandcAMP
   Molecular functionHydrolase
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processsignal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytosol

Inferred from Experiment. Source: Reactome

   Molecular function3',5'-cyclic-AMP phosphodiesterase activity

Inferred from Experiment. Source: Reactome

Complete GO annotation...

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]
Isoform PDE4C1 (identifier: Q08493-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform PDE4C2 (identifier: Q08493-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-106: Missing.
Isoform PDE4C3 (identifier: Q08493-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: MENLGVGEGA...LSWPVSSCRR → MQGPPAPAPV...RRFTVAHPLC
Isoform PDE4C4 (identifier: Q08493-4)

The sequence of this isoform is not available.
Isoform PDE4C5 (identifier: Q08493-5)

The sequence of this isoform is not available.
Isoform PDE4C6 (identifier: Q08493-6)

The sequence of this isoform is not available.
Isoform PDE4C7 (identifier: Q08493-7)

The sequence of this isoform is not available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 712712cAMP-specific 3',5'-cyclic phosphodiesterase 4C
PRO_0000198811

Amino acid modifications

Modified residue1641Phosphothreonine Ref.6
Modified residue6461Phosphothreonine Ref.5

Natural variations

Alternative sequence1 – 106106Missing in isoform PDE4C2.
VSP_004575
Alternative sequence1 – 8181MENLG…SSCRR → MQGPPAPAPVPGPGSPRGSP RGSPGLFRKLLVNQSIRLQR RFTVAHPLC in isoform PDE4C3.
VSP_004574
Natural variant1311S → L: dbSNP rs10413646.
VAR_050473
Natural variant2891R → Q: dbSNP rs34503849.
VAR_050474
Natural variant3441R → Q: dbSNP rs2229228.
VAR_034374

Experimental info

Sequence conflict2041K → N in AAD47053. Ref.2
Sequence conflict2041K → N in AAD47054. Ref.2
Sequence conflict2111D → Y in AAD47053. Ref.2
Sequence conflict2111D → Y in AAD47054. Ref.2
Sequence conflict339 – 3402EL → DV in CAA86601. Ref.1
Sequence conflict444 – 4463NSE → K in AAC83047. Ref.3
Sequence conflict446 – 4472EL → DV in CAA86601. Ref.1

Secondary structure

....................................................... 712
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform PDE4C1 [UniParc].

Last modified March 27, 2002. Version 2.
Checksum: 1932116C9CE0322C

FASTA71279,902
        10         20         30         40         50         60 
MENLGVGEGA EACSRLSRSR GRHSMTRAPK HLWRQPRRPI RIQQRFYSDP DKSAGCRERD 

        70         80         90        100        110        120 
LSPRPELRKS RLSWPVSSCR RFDLENGLSC GRRALDPQSS PGLGRIMQAP VPHSQRRESF 

       130        140        150        160        170        180 
LYRSDSDYEL SPKAMSRNSS VASDLHGEDM IVTPFAQVLA SLRTVRSNVA ALARQQCLGA 

       190        200        210        220        230        240 
AKQGPVGNPS SSNQLPPAED TGQKLALETL DELDWCLDQL ETLQTRHSVG EMASNKFKRI 

       250        260        270        280        290        300 
LNRELTHLSE TSRSGNQVSE YISRTFLDQQ TEVELPKVTA EEAPQPMSRI SGLHGLCHSA 

       310        320        330        340        350        360 
SLSSATVPRF GVQTDQEEQL AKELEDTNKW GLDVFKVAEL SGNRPLTAII FSIFQERDLL 

       370        380        390        400        410        420 
KTFQIPADTL ATYLLMLEGH YHANVAYHNS LHAADVAQST HVLLATPALE AVFTDLEILA 

       430        440        450        460        470        480 
ALFASAIHDV DHPGVSNQFL INTNSELALM YNDASVLENH HLAVGFKLLQ AENCDIFQNL 

       490        500        510        520        530        540 
SAKQRLSLRR MVIDMVLATD MSKHMNLLAD LKTMVETKKV TSLGVLLLDN YSDRIQVLQN 

       550        560        570        580        590        600 
LVHCADLSNP TKPLPLYRQW TDRIMAEFFQ QGDRERESGL DISPMCDKHT ASVEKSQVGF 

       610        620        630        640        650        660 
IDYIAHPLWE TWADLVHPDA QDLLDTLEDN REWYQSKIPR SPSDLTNPER DGPDRFQFEL 

       670        680        690        700        710 
TLEEAEEEDE EEEEEGEETA LAKEALELPD TELLSPEAGP DPGDLPLDNQ RT 

« Hide

Isoform PDE4C2.

Checksum: 3C42FC9F237333E1
Show »

FASTA60667,791
Isoform PDE4C3.

Checksum: E555883F32D17B64
Show »

FASTA68075,614
Isoform PDE4C4 (Sequence not available). FASTA
Isoform PDE4C5 (Sequence not available). FASTA
Isoform PDE4C6 (Sequence not available). FASTA
Isoform PDE4C7 (Sequence not available). FASTA

References

« Hide 'large scale' references
[1]"Molecular cloning and functional expression in yeast of a human cAMP-specific phosphodiesterase subtype (PDE IV-C)."
Engels P., Sullivan M., Mueller T., Luebbert H.
FEBS Lett. 358:305-310(1995) [PubMed: 7843419] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PDE4C1).
Tissue: Substantia nigra.
[2]"Genomic organisation of the human cyclic AMP-specific phosphodiesterase PDE4C gene and its chromosomal localisation to 19p13.1, between RAB3A and JUND."
Sullivan M., Olsen A.S., Houslay M.D.
Cell. Signal. 11:735-742(1999) [PubMed: 10574328] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS PDE4C1; PDE4C2 AND PDE4C3).
[3]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed: 15057824] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"A family of human phosphodiesterases homologous to the dunce learning and memory gene product of Drosophila melanogaster are potential targets for antidepressant drugs."
Bolger G., Michaeli T., Martins T., St John T., Steiner B., Rodgers L., Riggs M., Wigler M., Ferguson K.
Mol. Cell. Biol. 13:6558-6571(1993) [PubMed: 8413254] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 462-712.
[5]"Large-scale characterization of HeLa cell nuclear phosphoproteins."
Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-646, MASS SPECTROMETRY.
Tissue: Epithelium.
[6]"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column."
Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y.
Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-164, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

Z46632 mRNA. Translation: CAA86601.1.
AF157816 expand/collapse EMBL AC list , AF157811, AF157814, AF157815 Genomic DNA. Translation: AAD47053.1.
AF157816 expand/collapse EMBL AC list , AF157812, AF157814, AF157815 Genomic DNA. Translation: AAD47054.1.
AF157816, AF157814, AF157815 Genomic DNA. Translation: AAD47055.1.
AC005759 Genomic DNA. Translation: AAC83047.1.
L20968 mRNA. Translation: AAA03591.1.
IPIIPI00298070.
IPI00328206.
IPI00472785.
PIRS71626.
RefSeqNP_000914.2.
UniGeneHs.132584

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1LXUmodel-A306-649[»]
2QYMX-ray1.90A306-663[»]
ModBaseSearch...

Protein-protein interaction databases

STRINGQ08493.

PTM databases

PhosphoSiteQ08493.

Proteomic databases

PRIDEQ08493.

Genome annotation databases

EnsemblENST00000262805; ENSP00000262805; ENSG00000105650; Homo sapiens. [Genome view]
ENST00000336173; ENSP00000336624; ENSG00000105650; Homo sapiens. [Genome view]
ENST00000355502; ENSP00000347689; ENSG00000105650; Homo sapiens. [Genome view]
ENST00000447275; ENSP00000402091; ENSG00000105650; Homo sapiens. [Genome view]
GeneID5143.
KEGGhsa:5143.
UCSCuc002nik.2. human.

Organism-specific databases

CTD5143.
GeneCardsGC19M018181.
H-InvDBHIX0014907.
HGNCHGNC:8782. PDE4C.
MIM600128. gene.
PharmGKBPA264.
GenAtlasSearch...

Phylogenomic databases

HOVERGENQ08493.
OMAAIMFSIF.

Enzyme and pathway databases

BRENDA3.1.4.17. 247.
ReactomeREACT_14797. Signaling by GPCR.
REACT_15295. Opioid Signalling.

Gene expression databases

ArrayExpressQ08493.
BgeeQ08493.
CleanExHS_PDE4C.
GenevestigatorQ08493.
GermOnlineENSG00000105650. Homo sapiens.

Family and domain databases

InterProIPR003607. Met-dep_phosphohydro_HD.
IPR002073. PDEase.
[Graphical view]
PfamPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00471. HDc. 1 hit.
[Graphical view]
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

DrugBankDB00651. Dyphylline.
NextBio19838.
SOURCESearch...

Entry information

Entry namePDE4C_HUMAN
AccessionPrimary (citable) accession number: Q08493
Secondary accession number(s): Q9UN44 expand/collapse secondary AC list , Q9UN45, Q9UN46, Q9UPJ6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 27, 2002
Last modified: November 3, 2009
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents