ID   BUD21_YEAST             Reviewed;         214 AA.
AC   Q08492; O00033;
DT   01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 60.
DE   RecName: Full=Bud site selection protein 21;
DE   AltName: Full=U3 small nucleolar RNA-associated protein 16;
DE            Short=U3 snoRNA-associated protein 16;
DE   AltName: Full=U three protein 16;
GN   Name=BUD21; Synonyms=UTP16; OrderedLocusNames=YOR078W;
GN   ORFNames=YOR29-29;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=97279235; PubMed=9133743;
RX   DOI=10.1002/(SICI)1097-0061(19970330)13:4<379::AID-YEA85>3.3.CO;2-7;
RA   Valens M., Bohn C., Daignan-Fornier B., Dang V.-D.,
RA   Bolotin-Fukuhara M.;
RT   "The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the
RT   presence of two tRNAs and 24 new open reading frames.";
RL   Yeast 13:379-390(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   MEDLINE=97313270; PubMed=9169874;
RA   Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W.,
RA   Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R.,
RA   Boyer J., Camasses A., Casamayor A., Casas C., Cheret G.,
RA   Cziepluch C., Daignan-Fornier B., Dang V.-D., de Haan M., Delius H.,
RA   Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F.,
RA   Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A.,
RA   Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA   Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA   Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA   Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J.,
RA   Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P.,
RA   Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M.,
RA   Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R.,
RA   Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S.,
RA   Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A.,
RA   Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M.,
RA   Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C.,
RA   Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S.,
RA   Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL   Nature 387:98-102(1997).
RN   [3]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   MEDLINE=21345175; PubMed=11452010;
RA   Ni L., Snyder M.;
RT   "A genomic study of the bipolar bud site selection pattern in
RT   Saccharomyces cerevisiae.";
RL   Mol. Biol. Cell 12:2147-2170(2001).
RN   [4]
RP   FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU
RP   PROCESSOME BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX   MEDLINE=22082292; PubMed=12068309; DOI=10.1038/nature00769;
RA   Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M.,
RA   Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E.,
RA   Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J.;
RT   "A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA
RT   biogenesis.";
RL   Nature 417:967-970(2002).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-144, AND MASS
RP   SPECTROMETRY.
RX   PubMed=17330950; DOI=10.1021/pr060559j;
RA   Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA   Elias J.E., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of alpha-factor-arrested
RT   Saccharomyces cerevisiae.";
RL   J. Proteome Res. 6:1190-1197(2007).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39; SER-40; SER-41 AND
RP   SER-45, AND MASS SPECTROMETRY.
RX   PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA   Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA   Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT   "Analysis of phosphorylation sites on proteins from Saccharomyces
RT   cerevisiae by electron transfer dissociation (ETD) mass
RT   spectrometry.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-144, AND MASS
RP   SPECTROMETRY.
RX   PubMed=17563356; DOI=10.1073/pnas.0701622104;
RA   Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.;
RT   "Proteome-wide identification of in vivo targets of DNA damage
RT   checkpoint kinases.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65 AND SER-144, AND MASS
RP   SPECTROMETRY.
RX   PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth
RT   phosphoproteome analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
CC   -!- FUNCTION: Involved in nucleolar processing of pre-18S ribosomal
CC       RNA. Has a role in bud site selection maybe via the regulation of
CC       expression of bipolar budding components.
CC   -!- SUBUNIT: Interacts with snoRNA U3. Interacts with MPP10. Component
CC       of the ribosomal small subunit (SSU) processome composed of at
CC       least 40 protein subunits and snoRNA U3.
CC   -!- INTERACTION:
CC       P22696:ESS1; NbExp=1; IntAct=EBI-37880, EBI-6679;
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus.
CC   -!- MISCELLANEOUS: Present with 2120 molecules/cell in log phase SD
CC       medium.
CC   -!- SIMILARITY: Belongs to the UTP16 family.
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DR   EMBL; Z74986; CAA99271.1; -; Genomic_DNA.
DR   EMBL; Z70678; CAA94563.1; -; Genomic_DNA.
DR   PIR; S66961; S66961.
DR   RefSeq; NP_014721.1; -.
DR   DIP; DIP:1392N; -.
DR   IntAct; Q08492; 56.
DR   Ensembl; YOR078W; Saccharomyces cerevisiae.
DR   GeneID; 854245; -.
DR   GenomeReviews; Y13140_GR; YOR078W.
DR   KEGG; sce:YOR078W; -.
DR   NMPDR; fig|4932.3.peg.5824; -.
DR   CYGD; YOR078w; -.
DR   SGD; S000005604; BUD21.
DR   HOGENOM; Q08492; -.
DR   OMA; Q08492; PEEESTS.
DR   NextBio; 976153; -.
DR   GermOnline; YOR078W; Saccharomyces cerevisiae.
DR   GO; GO:0030686; C:90S preribosome; IDA:SGD.
DR   GO; GO:0005730; C:nucleolus; IDA:SGD.
DR   GO; GO:0032040; C:small-subunit processome; IDA:SGD.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0030515; F:snoRNA binding; IPI:SGD.
DR   GO; GO:0000447; P:endonucleolytic cleavage in ITS1 to separat...; IMP:SGD.
DR   GO; GO:0000472; P:endonucleolytic cleavage to generate mature...; IMP:SGD.
DR   InterPro; IPR013268; U3_snoRNA_assoc.
DR   Pfam; PF08297; U3_snoRNA_assoc; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Nucleus; Phosphoprotein; Ribonucleoprotein;
KW   Ribosome biogenesis; rRNA processing.
FT   CHAIN         1    214       Bud site selection protein 21.
FT                                /FTId=PRO_0000065013.
FT   MOD_RES      39     39       Phosphoserine.
FT   MOD_RES      40     40       Phosphoserine.
FT   MOD_RES      41     41       Phosphoserine.
FT   MOD_RES      45     45       Phosphoserine.
FT   MOD_RES      65     65       Phosphoserine.
FT   MOD_RES     144    144       Phosphoserine.
SQ   SEQUENCE   214 AA;  24385 MW;  6840F16B355693E4 CRC64;
     MSNGHVKFDA DESQASASAV TDRQDDVLVI SKKDKEVHSS SDEESDDDDA PQEEGLHSGK
     SEVESQITQR EEAIRLEQSQ LRSKRRKQNE LYAKQKKSVN ETEVTDEVIA ELPEELLKNI
     DQKDEGSTQY SSSRHVTFDK LDESDENEEA LAKAIKTKKR KTLKNLRKDS VKRGKFRVQL
     LSTTQDSKTL PPKKESSIIR SKDRWLNRKA LNKG
//