Bud site selection protein 21 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q08492 (BUD21_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 83. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Bud site selection protein 21

Alternative name(s):
U three protein 16
U3 small nucleolar RNA-associated protein 16
Short name=U3 snoRNA-associated protein 16

Gene names

Name:	BUD21
Synonyms:	UTP16
Ordered Locus Names:	YOR078W
ORF Names:	YOR29-29

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in bud site selection maybe via the regulation of expression of bipolar budding components. Ref.4 Ref.5
Subunit structure	Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.5
Subcellular location	Nucleus › nucleolus Ref.4 Ref.5.
Miscellaneous	Present with 2120 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the UTP16 family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Molecular function	Ribonucleoprotein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD
Cellular component	90S preribosome Inferred from direct assay. Source: SGD nucleolus Inferred from direct assay Ref.5. Source: SGD small-subunit processome Inferred from direct assay Ref.5. Source: SGD
Molecular function	snoRNA binding Inferred from physical interaction Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ESS1	P22696	1	EBI-37880,EBI-6679

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 214

214

Bud site selection protein 21

PRO_0000065013

Amino acid modifications

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphoserine Ref.10

Modified residue

144

Phosphoserine Ref.7 Ref.9 Ref.10

Sequences

Sequence

Length

Mass (Da)

Tools

Q08492 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 6840F16B355693E4
Show »

FASTA

214

24,385

        10         20         30         40         50         60 
MSNGHVKFDA DESQASASAV TDRQDDVLVI SKKDKEVHSS SDEESDDDDA PQEEGLHSGK 

        70         80         90        100        110        120 
SEVESQITQR EEAIRLEQSQ LRSKRRKQNE LYAKQKKSVN ETEVTDEVIA ELPEELLKNI 

       130        140        150        160        170        180 
DQKDEGSTQY SSSRHVTFDK LDESDENEEA LAKAIKTKKR KTLKNLRKDS VKRGKFRVQL 

       190        200        210 
LSTTQDSKTL PPKKESSIIR SKDRWLNRKA LNKG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames." Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M. Yeast 13:379-390(1997) [PubMed: 9133743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae." Ni L., Snyder M. Mol. Biol. Cell 12:2147-2170(2001) [PubMed: 11452010] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis." Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M., Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J. Nature 417:967-970(2002) [PubMed: 12068309] [Abstract] Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-144, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39; SER-40; SER-41 AND SER-45, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-144, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65 AND SER-144, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z74986 Genomic DNA. Translation: CAA99271.1. Z70678 Genomic DNA. Translation: CAA94563.1. BK006948 Genomic DNA. Translation: DAA10857.1.
PIR	S66961.
RefSeq	NP_014721.1. NM_001183497.1.
3D structure databases
ProteinModelPortal	Q08492.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1392N.
IntAct	Q08492. 35 interactions.
MINT	MINT-402119.
STRING	Q08492.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR078W; YOR078W; YOR078W.
GeneID	854245.
KEGG	sce:YOR078W.
NMPDR	fig\|4932.3.peg.5824.
Organism-specific databases
CYGD	YOR078w.
SGD	S000005604. BUD21.
Phylogenomic databases
eggNOG	fuNOG11967.
OMA	KSKDKWL.
OrthoDB	EOG42FWTD.
Gene expression databases
ArrayExpress	Q08492.
Genevestigator	Q08492.
GermOnline	YOR078W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013268. U3_snoRNA_assoc. [Graphical view]
KO	K14770.
Pfam	PF08297. U3_snoRNA_assoc. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976153.

Entry information

Entry name

BUD21_YEAST

Accession

Primary (citable) accession number: Q08492
Secondary accession number(s): D6W2E1, O00033

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents