Reviewed, UniProtKB/Swiss-Prot Q08492 (BUD21_YEAST)

Names and origin

Protein names

Recommended name:
    Bud site selection protein 21
Alternative name(s):
    U3 small nucleolar RNA-associated protein 16
      Short name=U3 snoRNA-associated protein 16
    U three protein 16

Gene names

Name:	BUD21
Synonyms:	UTP16
Ordered Locus Names:	YOR078W
ORF Names:	YOR29-29

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in bud site selection maybe via the regulation of expression of bipolar budding components. Ref.3 Ref.4
Subunit structure	Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.4
Subcellular location	Nucleus › nucleolus. Ref.3 Ref.4
Miscellaneous	Present with 2120 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the UTP16 family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Molecular function	Ribonucleoprotein
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD
Cellular component	90S preribosome Inferred from direct assay. Source: SGD nucleolus Ref.4 Inferred from direct assay. Source: SGD small-subunit processome Ref.4 Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct snoRNA binding Ref.4 Inferred from physical interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ESS1	P22696	1	EBI-37880,EBI-6679

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 214	214	Bud site selection protein 21		PRO_0000065013
Amino acid modifications
	Modified residue	39	1	Phosphoserine Ref.7
	Modified residue	40	1	Phosphoserine Ref.7
	Modified residue	41	1	Phosphoserine Ref.7
	Modified residue	45	1	Phosphoserine Ref.7
	Modified residue	65	1	Phosphoserine Ref.9
	Modified residue	144	1	Phosphoserine Ref.9 Ref.6 Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q08492-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6840F16B355693E4	FASTA	214	24,385
	10 20 30 40 50 60 MSNGHVKFDA DESQASASAV TDRQDDVLVI SKKDKEVHSS SDEESDDDDA PQEEGLHSGK 70 80 90 100 110 120 SEVESQITQR EEAIRLEQSQ LRSKRRKQNE LYAKQKKSVN ETEVTDEVIA ELPEELLKNI 130 140 150 160 170 180 DQKDEGSTQY SSSRHVTFDK LDESDENEEA LAKAIKTKKR KTLKNLRKDS VKRGKFRVQL 190 200 210 LSTTQDSKTL PPKKESSIIR SKDRWLNRKA LNKG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames." Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M. Yeast 13:379-390(1997) [PubMed: 9133743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae." Ni L., Snyder M. Mol. Biol. Cell 12:2147-2170(2001) [PubMed: 11452010] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]	"A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis." Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M., Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J. Nature 417:967-970(2002) [PubMed: 12068309] [Abstract] Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-144, MASS SPECTROMETRY.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39; SER-40; SER-41 AND SER-45, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-144, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65 AND SER-144, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z74986 Genomic DNA. Translation: CAA99271.1. Z70678 Genomic DNA. Translation: CAA94563.1.
PIR	S66961.
RefSeq	NP_014721.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1392N.
IntAct	Q08492. 56 interactions.
Genome annotation databases
Ensembl	YOR078W. Saccharomyces cerevisiae. [Contig view]
GeneID	854245.
GenomeReviews	Gene locus YOR078W in contig Y13140_GR.
KEGG	sce:YOR078W.
NMPDR	fig|4932.3.peg.5824.
Organism-specific databases
CYGD	YOR078w.
SGD	S000005604. BUD21.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q08492.
OMA	Q08492. PEEESTS.
Gene expression databases
GermOnline	YOR078W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013268. U3_snoRNA_assoc. [Graphical view]
Pfam	PF08297. U3_snoRNA_assoc. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	976153.

Entry information

Entry name

BUD21_YEAST

Accession

Primary (citable) accession number: Q08492
Secondary accession number(s): O00033

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)