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Q08491

- SKI7_YEAST

UniProt

Q08491 - SKI7_YEAST

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Protein

Superkiller protein 7

Gene

SKI7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Represses the expression of non-poly(A) mRNAs like L-A or M viruses and is therefore involved in antiviral system. Mediates interactions via its N-terminus between the exosome and the SKI complex which operate in the 3'-to-5' mRNA-decay pathway. By interacting with NAM7, is also required for nonsense-mediated 3'-to-5' mRNA-decay (NMD). May recognize a stalled 80S ribosome at the 3'-end of a nonstop mRNA which leads to the recruitment of the exosome and SKI complexes to the mRNAs to be degraded.10 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi274 – 2818GTPBy similarity
Nucleotide bindingi356 – 3605GTPBy similarity
Nucleotide bindingi427 – 4304GTPBy similarity

GO - Molecular functioni

  1. GTPase activity Source: InterPro
  2. GTP binding Source: UniProtKB-KW
  3. protein binding, bridging Source: SGD

GO - Biological processi

  1. nonfunctional rRNA decay Source: SGD
  2. nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay Source: SGD
  3. nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' Source: SGD
  4. nuclear-transcribed mRNA catabolic process, non-stop decay Source: SGD
  5. regulation of translation Source: UniProtKB-KW
  6. translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Nonsense-mediated mRNA decay, Protein biosynthesis, Translation regulation

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33613-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Superkiller protein 7
Gene namesi
Name:SKI7
Ordered Locus Names:YOR076C
ORF Names:YOR29-27
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR076c.
SGDiS000005602. SKI7.

Subcellular locationi

Cytoplasm 2 Publications

GO - Cellular componenti

  1. cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 747747Superkiller protein 7PRO_0000269648Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei88 – 881Phosphoserine1 Publication
Modified residuei90 – 901Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08491.
PaxDbiQ08491.
PeptideAtlasiQ08491.

Expressioni

Gene expression databases

GenevestigatoriQ08491.

Interactioni

Subunit structurei

Interacts with the exosome and with the SKI complex composed of at least SKI2, SKI3 and SKI8. Interacts directly with SKI3 and SKI8.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CSL4P538595EBI-1389,EBI-1731
SKI3P178832EBI-1389,EBI-1861
SKI8Q027933EBI-1389,EBI-17260
UPF1Q929002EBI-1389,EBI-373471From a different organism.

Protein-protein interaction databases

BioGridi34475. 104 interactions.
DIPiDIP-995N.
IntActiQ08491. 17 interactions.
MINTiMINT-518978.

Structurei

3D structure databases

ProteinModelPortaliQ08491.
SMRiQ08491. Positions 249-566.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini265 – 503239tr-type GPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni274 – 2818G1PROSITE-ProRule annotation
Regioni331 – 3355G2PROSITE-ProRule annotation
Regioni356 – 3594G3PROSITE-ProRule annotation
Regioni427 – 4304G4PROSITE-ProRule annotation
Regioni467 – 4693G5PROSITE-ProRule annotation

Domaini

The N-terminal domain (residues 1 to 264) is required and sufficient for interaction with the exosome and SKI complexes and for 3'-to-5' mRNA degradation.1 Publication

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5256.
GeneTreeiENSGT00600000085333.
InParanoidiQ08491.
KOiK12595.
OrthoDBiEOG7V1G03.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08491-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLLEQLARK RIEKSKGLLS ADQSHSTSKS ASLLERLHKN RETKDNNAET
60 70 80 90 100
KRKDLKTLLA KDKVKRSDFT PNQHSVSLSL KLSALKKSNS DLEKQGKSVT
110 120 130 140 150
LDSKENELPT KRKSPDDKLN LEESWKAIKE MNHYCFLKND PCINQTDDFA
160 170 180 190 200
FTNFIIKDKK NSLSTSIPLS SQNSSFLSLK KHNNELLGIF VPCNLPKTTR
210 220 230 240 250
KVAIENFNRP SPDDIIQSAQ LNAFNEKLEN LNIKSVPKAE KKEPINLQTP
260 270 280 290 300
PTESIDIHSF IATHPLNLTC LFLGDTNAGK STLLGHLLYD LNEISMSSMR
310 320 330 340 350
ELQKKSSNLD PSSSNSFKVI LDNTKTEREN GFSMFKKVIQ VENDLLPPSS
360 370 380 390 400
TLTLIDTPGS IKYFNKETLN SILTFDPEVY VLVIDCNYDS WEKSLDGPNN
410 420 430 440 450
QIYEILKVIS YLNKNSACKK HLIILLNKAD LISWDKHRLE MIQSELNYVL
460 470 480 490 500
KENFQWTDAE FQFIPCSGLL GSNLNKTENI TKSKYKSEFD SINYVPEWYE
510 520 530 540 550
GPTFFSQLYL LVEHNMNKIE TTLEEPFVGT ILQSSVLQPI AEINYVSLKV
560 570 580 590 600
LINSGYIQSG QTIEIHTQYE DFHYYGIVSR MKNSKQILET NTKNNISVGL
610 620 630 640 650
NPDILEVLVK IHNTEDFTKK QFHIRKGDII IHSRKTNTLS PNLPNTLKLL
660 670 680 690 700
ALRLIKLSIQ THALSDPVDL GSELLLYHNL THNAVKLVKI LGTNDISINP
710 720 730 740
NQSLIVEVEI IEPDFALNVI DSKYITNNIV LTSIDHKVIA VGRIACQ
Length:747
Mass (Da):84,779
Last modified:November 1, 1996 - v1
Checksum:iD93B956D02050BFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70678 Genomic DNA. Translation: CAA94561.1.
Z74984 Genomic DNA. Translation: CAA99269.1.
BK006948 Genomic DNA. Translation: DAA10855.1.
PIRiS66959.
RefSeqiNP_014719.1. NM_001183495.1.

Genome annotation databases

EnsemblFungiiYOR076C; YOR076C; YOR076C.
GeneIDi854243.
KEGGisce:YOR076C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70678 Genomic DNA. Translation: CAA94561.1 .
Z74984 Genomic DNA. Translation: CAA99269.1 .
BK006948 Genomic DNA. Translation: DAA10855.1 .
PIRi S66959.
RefSeqi NP_014719.1. NM_001183495.1.

3D structure databases

ProteinModelPortali Q08491.
SMRi Q08491. Positions 249-566.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34475. 104 interactions.
DIPi DIP-995N.
IntActi Q08491. 17 interactions.
MINTi MINT-518978.

Proteomic databases

MaxQBi Q08491.
PaxDbi Q08491.
PeptideAtlasi Q08491.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR076C ; YOR076C ; YOR076C .
GeneIDi 854243.
KEGGi sce:YOR076C.

Organism-specific databases

CYGDi YOR076c.
SGDi S000005602. SKI7.

Phylogenomic databases

eggNOGi COG5256.
GeneTreei ENSGT00600000085333.
InParanoidi Q08491.
KOi K12595.
OrthoDBi EOG7V1G03.

Enzyme and pathway databases

BioCyci YEAST:G3O-33613-MONOMER.

Miscellaneous databases

NextBioi 976147.

Gene expression databases

Genevestigatori Q08491.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00009. GTP_EFTU. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS51722. G_TR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames."
    Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M.
    Yeast 13:379-390(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Superkiller mutations in Saccharomyces cerevisiae suppress exclusion of M2 double-stranded RNA by L-A-HN and confer cold sensitivity in the presence of M and L-A-HN."
    Ridley S.P., Sommer S.S., Wickner R.B.
    Mol. Cell. Biol. 4:761-770(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "The ski7 antiviral protein is an EF1-alpha homolog that blocks expression of non-Poly(A) mRNA in Saccharomyces cerevisiae."
    Benard L., Carroll K., Valle R.C.P., Masison D.C., Wickner R.B.
    J. Virol. 73:2893-2900(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Function of the ski4p (Csl4p) and Ski7p proteins in 3'-to-5' degradation of mRNA."
    van Hoof A., Staples R.R., Baker R.E., Parker R.
    Mol. Cell. Biol. 20:8230-8243(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Ski7p G protein interacts with the exosome and the Ski complex for 3'-to-5' mRNA decay in yeast."
    Araki Y., Takahashi S., Kobayashi T., Kajiho H., Hoshino S., Katada T.
    EMBO J. 20:4684-4693(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH THE EXOSOME AND SKI COMPLEXES.
  8. "The yeast cytoplasmic LsmI/Pat1p complex protects mRNA 3' termini from partial degradation."
    He W., Parker R.
    Genetics 158:1445-1455(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Exosome-mediated recognition and degradation of mRNAs lacking a termination codon."
    van Hoof A., Frischmeyer P.A., Dietz H.C., Parker R.
    Science 295:2262-2264(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH THE EXOSOME COMPLEX.
  10. "Interaction between Ski7p and Upf1p is required for nonsense-mediated 3'-to-5' mRNA decay in yeast."
    Takahashi S., Araki Y., Sakuno T., Katada T.
    EMBO J. 22:3951-3959(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NAM7.
  11. "An NMD pathway in yeast involving accelerated deadenylation and exosome-mediated 3'-->5' degradation."
    Mitchell P., Tollervey D.
    Mol. Cell 11:1405-1413(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  13. "Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus."
    Kushner D.B., Lindenbach B.D., Grdzelishvili V.Z., Noueiry A.O., Paul S.M., Ahlquist P.
    Proc. Natl. Acad. Sci. U.S.A. 100:15764-15769(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "Decapping and decay of messenger RNA occur in cytoplasmic processing bodies."
    Sheth U., Parker R.
    Science 300:805-808(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  15. "Translation of aberrant mRNAs lacking a termination codon or with a shortened 3'-UTR is repressed after initiation in yeast."
    Inada T., Aiba H.
    EMBO J. 24:1584-1595(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  16. "Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p."
    Wang L., Lewis M.S., Johnson A.W.
    RNA 11:1291-1302(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SKI3 AND SKI8.
  17. "A single subunit, Dis3, is essentially responsible for yeast exosome core activity."
    Dziembowski A., Lorentzen E., Conti E., Seraphin B.
    Nat. Struct. Mol. Biol. 14:15-22(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH THE EXOSOME.
  18. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-88 AND SER-90, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSKI7_YEAST
AccessioniPrimary (citable) accession number: Q08491
Secondary accession number(s): D6W2D9, O00032
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 233 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3