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Q08491 (SKI7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Superkiller protein 7
Gene names
Name:SKI7
Ordered Locus Names:YOR076C
ORF Names:YOR29-27
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length747 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Represses the expression of non-poly(A) mRNAs like L-A or M viruses and is therefore involved in antiviral system. Mediates interactions via its N-terminus between the exosome and the SKI complex which operate in the 3'-to-5' mRNA-decay pathway. By interacting with NAM7, is also required for nonsense-mediated 3'-to-5' mRNA-decay (NMD). May recognize a stalled 80S ribosome at the 3'-end of a nonstop mRNA which leads to the recruitment of the exosome and SKI complexes to the mRNAs to be degraded. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.15

Subunit structure

Interacts with the exosome and with the SKI complex composed of at least SKI2, SKI3 and SKI8. Interacts directly with SKI3 and SKI8. Ref.7 Ref.9 Ref.10 Ref.16 Ref.17

Subcellular location

Cytoplasm Ref.7 Ref.14.

Domain

The N-terminal domain (residues 1 to 264) is required and sufficient for interaction with the exosome and SKI complexes and for 3'-to-5' mRNA degradation. Ref.7

Miscellaneous

Present with 233 molecules/cell in log phase SD medium.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CSL4P538595EBI-1389,EBI-1731
SKI3P178832EBI-1389,EBI-1861
SKI8Q027933EBI-1389,EBI-17260
UPF1Q929002EBI-1389,EBI-373471From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 747747Superkiller protein 7
PRO_0000269648

Regions

Nucleotide binding274 – 2818GTP By similarity
Nucleotide binding356 – 3605GTP By similarity
Nucleotide binding427 – 4304GTP By similarity

Amino acid modifications

Modified residue881Phosphoserine Ref.18
Modified residue901Phosphoserine Ref.18

Sequences

Sequence LengthMass (Da)Tools
Q08491 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D93B956D02050BFA

FASTA74784,779
        10         20         30         40         50         60 
MSLLEQLARK RIEKSKGLLS ADQSHSTSKS ASLLERLHKN RETKDNNAET KRKDLKTLLA 

        70         80         90        100        110        120 
KDKVKRSDFT PNQHSVSLSL KLSALKKSNS DLEKQGKSVT LDSKENELPT KRKSPDDKLN 

       130        140        150        160        170        180 
LEESWKAIKE MNHYCFLKND PCINQTDDFA FTNFIIKDKK NSLSTSIPLS SQNSSFLSLK 

       190        200        210        220        230        240 
KHNNELLGIF VPCNLPKTTR KVAIENFNRP SPDDIIQSAQ LNAFNEKLEN LNIKSVPKAE 

       250        260        270        280        290        300 
KKEPINLQTP PTESIDIHSF IATHPLNLTC LFLGDTNAGK STLLGHLLYD LNEISMSSMR 

       310        320        330        340        350        360 
ELQKKSSNLD PSSSNSFKVI LDNTKTEREN GFSMFKKVIQ VENDLLPPSS TLTLIDTPGS 

       370        380        390        400        410        420 
IKYFNKETLN SILTFDPEVY VLVIDCNYDS WEKSLDGPNN QIYEILKVIS YLNKNSACKK 

       430        440        450        460        470        480 
HLIILLNKAD LISWDKHRLE MIQSELNYVL KENFQWTDAE FQFIPCSGLL GSNLNKTENI 

       490        500        510        520        530        540 
TKSKYKSEFD SINYVPEWYE GPTFFSQLYL LVEHNMNKIE TTLEEPFVGT ILQSSVLQPI 

       550        560        570        580        590        600 
AEINYVSLKV LINSGYIQSG QTIEIHTQYE DFHYYGIVSR MKNSKQILET NTKNNISVGL 

       610        620        630        640        650        660 
NPDILEVLVK IHNTEDFTKK QFHIRKGDII IHSRKTNTLS PNLPNTLKLL ALRLIKLSIQ 

       670        680        690        700        710        720 
THALSDPVDL GSELLLYHNL THNAVKLVKI LGTNDISINP NQSLIVEVEI IEPDFALNVI 

       730        740 
DSKYITNNIV LTSIDHKVIA VGRIACQ 

« Hide

References

« Hide 'large scale' references
[1]"The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames."
Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M.
Yeast 13:379-390(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Superkiller mutations in Saccharomyces cerevisiae suppress exclusion of M2 double-stranded RNA by L-A-HN and confer cold sensitivity in the presence of M and L-A-HN."
Ridley S.P., Sommer S.S., Wickner R.B.
Mol. Cell. Biol. 4:761-770(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"The ski7 antiviral protein is an EF1-alpha homolog that blocks expression of non-Poly(A) mRNA in Saccharomyces cerevisiae."
Benard L., Carroll K., Valle R.C.P., Masison D.C., Wickner R.B.
J. Virol. 73:2893-2900(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Function of the ski4p (Csl4p) and Ski7p proteins in 3'-to-5' degradation of mRNA."
van Hoof A., Staples R.R., Baker R.E., Parker R.
Mol. Cell. Biol. 20:8230-8243(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Ski7p G protein interacts with the exosome and the Ski complex for 3'-to-5' mRNA decay in yeast."
Araki Y., Takahashi S., Kobayashi T., Kajiho H., Hoshino S., Katada T.
EMBO J. 20:4684-4693(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH THE EXOSOME AND SKI COMPLEXES.
[8]"The yeast cytoplasmic LsmI/Pat1p complex protects mRNA 3' termini from partial degradation."
He W., Parker R.
Genetics 158:1445-1455(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Exosome-mediated recognition and degradation of mRNAs lacking a termination codon."
van Hoof A., Frischmeyer P.A., Dietz H.C., Parker R.
Science 295:2262-2264(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH THE EXOSOME COMPLEX.
[10]"Interaction between Ski7p and Upf1p is required for nonsense-mediated 3'-to-5' mRNA decay in yeast."
Takahashi S., Araki Y., Sakuno T., Katada T.
EMBO J. 22:3951-3959(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NAM7.
[11]"An NMD pathway in yeast involving accelerated deadenylation and exosome-mediated 3'-->5' degradation."
Mitchell P., Tollervey D.
Mol. Cell 11:1405-1413(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[13]"Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus."
Kushner D.B., Lindenbach B.D., Grdzelishvili V.Z., Noueiry A.O., Paul S.M., Ahlquist P.
Proc. Natl. Acad. Sci. U.S.A. 100:15764-15769(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[14]"Decapping and decay of messenger RNA occur in cytoplasmic processing bodies."
Sheth U., Parker R.
Science 300:805-808(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[15]"Translation of aberrant mRNAs lacking a termination codon or with a shortened 3'-UTR is repressed after initiation in yeast."
Inada T., Aiba H.
EMBO J. 24:1584-1595(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[16]"Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p."
Wang L., Lewis M.S., Johnson A.W.
RNA 11:1291-1302(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SKI3 AND SKI8.
[17]"A single subunit, Dis3, is essentially responsible for yeast exosome core activity."
Dziembowski A., Lorentzen E., Conti E., Seraphin B.
Nat. Struct. Mol. Biol. 14:15-22(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH THE EXOSOME.
[18]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-88 AND SER-90, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z70678 Genomic DNA. Translation: CAA94561.1.
Z74984 Genomic DNA. Translation: CAA99269.1.
BK006948 Genomic DNA. Translation: DAA10855.1.
PIRS66959.
RefSeqNP_014719.1. NM_001183495.1.

3D structure databases

ProteinModelPortalQ08491.
SMRQ08491. Positions 249-745.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34475. 104 interactions.
DIPDIP-995N.
IntActQ08491. 17 interactions.
MINTMINT-518978.

Proteomic databases

PaxDbQ08491.
PeptideAtlasQ08491.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR076C; YOR076C; YOR076C.
GeneID854243.
KEGGsce:YOR076C.

Organism-specific databases

CYGDYOR076c.
SGDS000005602. SKI7.

Phylogenomic databases

eggNOGCOG5256.
GeneTreeENSGT00600000085333.
KOK12595.
OMATERENGF.
OrthoDBEOG7V1G03.

Enzyme and pathway databases

BioCycYEAST:G3O-33613-MONOMER.

Gene expression databases

GenevestigatorQ08491.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00009. GTP_EFTU. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Other

NextBio976147.

Entry information

Entry nameSKI7_YEAST
AccessionPrimary (citable) accession number: Q08491
Secondary accession number(s): D6W2D9, O00032
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD