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Protein

Superkiller protein 7

Gene

SKI7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Represses the expression of non-poly(A) mRNAs like L-A or M viruses and is therefore involved in antiviral system. Mediates interactions via its N-terminus between the exosome and the SKI complex which operate in the 3'-to-5' mRNA-decay pathway. By interacting with NAM7, is also required for nonsense-mediated 3'-to-5' mRNA-decay (NMD). May recognize a stalled 80S ribosome at the 3'-end of a nonstop mRNA which leads to the recruitment of the exosome and SKI complexes to the mRNAs to be degraded.10 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi274 – 281GTPBy similarity8
Nucleotide bindingi356 – 360GTPBy similarity5
Nucleotide bindingi427 – 430GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: GO_Central
  • GTP binding Source: SGD
  • protein binding, bridging Source: SGD

GO - Biological processi

  • mitochondrial translational initiation Source: GO_Central
  • nonfunctional rRNA decay Source: SGD
  • nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay Source: SGD
  • nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' Source: SGD
  • nuclear-transcribed mRNA catabolic process, non-stop decay Source: SGD
  • regulation of translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Nonsense-mediated mRNA decay, Protein biosynthesis, Translation regulation

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33613-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Superkiller protein 7
Gene namesi
Name:SKI7
Ordered Locus Names:YOR076C
ORF Names:YOR29-27
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR076C.
SGDiS000005602. SKI7.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • mitochondrion Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002696481 – 747Superkiller protein 7Add BLAST747

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei88PhosphoserineCombined sources1
Modified residuei90PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08491.
PRIDEiQ08491.

PTM databases

iPTMnetiQ08491.

Interactioni

Subunit structurei

Interacts with the exosome and with the SKI complex composed of at least SKI2, SKI3 and SKI8. Interacts directly with SKI3 and SKI8.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CSL4P538595EBI-1389,EBI-1731
SKI3P178832EBI-1389,EBI-1861
SKI8Q027933EBI-1389,EBI-17260
UPF1Q929002EBI-1389,EBI-373471From a different organism.

GO - Molecular functioni

  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi34475. 105 interactors.
DIPiDIP-995N.
IntActiQ08491. 17 interactors.
MINTiMINT-518978.

Structurei

Secondary structure

1747
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi121 – 131Combined sources11
Helixi150 – 155Combined sources6
Beta strandi185 – 189Combined sources5
Helixi197 – 208Combined sources12
Helixi212 – 225Combined sources14
Helixi258 – 262Combined sources5
Beta strandi266 – 273Combined sources8
Helixi280 – 290Combined sources11
Helixi296 – 305Combined sources10
Helixi306 – 309Combined sources4
Turni311 – 313Combined sources3
Helixi318 – 321Combined sources4
Helixi325 – 330Combined sources6
Beta strandi337 – 341Combined sources5
Beta strandi350 – 356Combined sources7
Helixi361 – 364Combined sources4
Turni365 – 367Combined sources3
Helixi368 – 375Combined sources8
Beta strandi378 – 385Combined sources8
Helixi390 – 393Combined sources4
Helixi401 – 409Combined sources9
Turni410 – 415Combined sources6
Beta strandi422 – 427Combined sources6
Helixi429 – 432Combined sources4
Helixi436 – 452Combined sources17
Helixi458 – 460Combined sources3
Beta strandi461 – 465Combined sources5
Turni468 – 471Combined sources4
Helixi504 – 515Combined sources12
Turni522 – 524Combined sources3
Beta strandi528 – 536Combined sources9
Beta strandi542 – 558Combined sources17
Beta strandi562 – 566Combined sources5
Beta strandi568 – 571Combined sources4
Beta strandi573 – 583Combined sources11
Beta strandi593 – 595Combined sources3
Beta strandi597 – 600Combined sources4
Beta strandi604 – 612Combined sources9
Turni617 – 619Combined sources3
Beta strandi629 – 632Combined sources4
Beta strandi648 – 660Combined sources13
Beta strandi673 – 678Combined sources6
Beta strandi681 – 691Combined sources11
Beta strandi696 – 698Combined sources3
Beta strandi703 – 710Combined sources8
Beta strandi717 – 722Combined sources6
Beta strandi728 – 732Combined sources5
Beta strandi738 – 744Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ZKDX-ray2.18A254-747[»]
4ZKEX-ray2.25A254-747[»]
5G06electron microscopy4.20P1-747[»]
5JEAX-ray2.65K116-225[»]
ProteinModelPortaliQ08491.
SMRiQ08491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini265 – 503tr-type GPROSITE-ProRule annotationAdd BLAST239

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni274 – 281G1PROSITE-ProRule annotation8
Regioni331 – 335G2PROSITE-ProRule annotation5
Regioni356 – 359G3PROSITE-ProRule annotation4
Regioni427 – 430G4PROSITE-ProRule annotation4
Regioni467 – 469G5PROSITE-ProRule annotation3

Domaini

The N-terminal domain (residues 1 to 264) is required and sufficient for interaction with the exosome and SKI complexes and for 3'-to-5' mRNA degradation.1 Publication

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00600000085333.
InParanoidiQ08491.
KOiK12595.
OMAiTERENGF.
OrthoDBiEOG092C0R0J.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08491-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLEQLARK RIEKSKGLLS ADQSHSTSKS ASLLERLHKN RETKDNNAET
60 70 80 90 100
KRKDLKTLLA KDKVKRSDFT PNQHSVSLSL KLSALKKSNS DLEKQGKSVT
110 120 130 140 150
LDSKENELPT KRKSPDDKLN LEESWKAIKE MNHYCFLKND PCINQTDDFA
160 170 180 190 200
FTNFIIKDKK NSLSTSIPLS SQNSSFLSLK KHNNELLGIF VPCNLPKTTR
210 220 230 240 250
KVAIENFNRP SPDDIIQSAQ LNAFNEKLEN LNIKSVPKAE KKEPINLQTP
260 270 280 290 300
PTESIDIHSF IATHPLNLTC LFLGDTNAGK STLLGHLLYD LNEISMSSMR
310 320 330 340 350
ELQKKSSNLD PSSSNSFKVI LDNTKTEREN GFSMFKKVIQ VENDLLPPSS
360 370 380 390 400
TLTLIDTPGS IKYFNKETLN SILTFDPEVY VLVIDCNYDS WEKSLDGPNN
410 420 430 440 450
QIYEILKVIS YLNKNSACKK HLIILLNKAD LISWDKHRLE MIQSELNYVL
460 470 480 490 500
KENFQWTDAE FQFIPCSGLL GSNLNKTENI TKSKYKSEFD SINYVPEWYE
510 520 530 540 550
GPTFFSQLYL LVEHNMNKIE TTLEEPFVGT ILQSSVLQPI AEINYVSLKV
560 570 580 590 600
LINSGYIQSG QTIEIHTQYE DFHYYGIVSR MKNSKQILET NTKNNISVGL
610 620 630 640 650
NPDILEVLVK IHNTEDFTKK QFHIRKGDII IHSRKTNTLS PNLPNTLKLL
660 670 680 690 700
ALRLIKLSIQ THALSDPVDL GSELLLYHNL THNAVKLVKI LGTNDISINP
710 720 730 740
NQSLIVEVEI IEPDFALNVI DSKYITNNIV LTSIDHKVIA VGRIACQ
Length:747
Mass (Da):84,779
Last modified:November 1, 1996 - v1
Checksum:iD93B956D02050BFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70678 Genomic DNA. Translation: CAA94561.1.
Z74984 Genomic DNA. Translation: CAA99269.1.
BK006948 Genomic DNA. Translation: DAA10855.1.
PIRiS66959.
RefSeqiNP_014719.1. NM_001183495.1.

Genome annotation databases

EnsemblFungiiYOR076C; YOR076C; YOR076C.
GeneIDi854243.
KEGGisce:YOR076C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70678 Genomic DNA. Translation: CAA94561.1.
Z74984 Genomic DNA. Translation: CAA99269.1.
BK006948 Genomic DNA. Translation: DAA10855.1.
PIRiS66959.
RefSeqiNP_014719.1. NM_001183495.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ZKDX-ray2.18A254-747[»]
4ZKEX-ray2.25A254-747[»]
5G06electron microscopy4.20P1-747[»]
5JEAX-ray2.65K116-225[»]
ProteinModelPortaliQ08491.
SMRiQ08491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34475. 105 interactors.
DIPiDIP-995N.
IntActiQ08491. 17 interactors.
MINTiMINT-518978.

PTM databases

iPTMnetiQ08491.

Proteomic databases

MaxQBiQ08491.
PRIDEiQ08491.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR076C; YOR076C; YOR076C.
GeneIDi854243.
KEGGisce:YOR076C.

Organism-specific databases

EuPathDBiFungiDB:YOR076C.
SGDiS000005602. SKI7.

Phylogenomic databases

GeneTreeiENSGT00600000085333.
InParanoidiQ08491.
KOiK12595.
OMAiTERENGF.
OrthoDBiEOG092C0R0J.

Enzyme and pathway databases

BioCyciYEAST:G3O-33613-MONOMER.

Miscellaneous databases

PROiQ08491.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSKI7_YEAST
AccessioniPrimary (citable) accession number: Q08491
Secondary accession number(s): D6W2D9, O00032
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 233 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.