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Protein

Superkiller protein 7

Gene

SKI7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Represses the expression of non-poly(A) mRNAs like L-A or M viruses and is therefore involved in antiviral system. Mediates interactions via its N-terminus between the exosome and the SKI complex which operate in the 3'-to-5' mRNA-decay pathway. By interacting with NAM7, is also required for nonsense-mediated 3'-to-5' mRNA-decay (NMD). May recognize a stalled 80S ribosome at the 3'-end of a nonstop mRNA which leads to the recruitment of the exosome and SKI complexes to the mRNAs to be degraded.10 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi274 – 2818GTPBy similarity
Nucleotide bindingi356 – 3605GTPBy similarity
Nucleotide bindingi427 – 4304GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: GO_Central
  • GTP binding Source: SGD
  • protein binding, bridging Source: SGD

GO - Biological processi

  • mitochondrial translational initiation Source: GO_Central
  • nonfunctional rRNA decay Source: SGD
  • nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay Source: SGD
  • nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' Source: SGD
  • nuclear-transcribed mRNA catabolic process, non-stop decay Source: SGD
  • regulation of translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Nonsense-mediated mRNA decay, Protein biosynthesis, Translation regulation

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33613-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Superkiller protein 7
Gene namesi
Name:SKI7
Ordered Locus Names:YOR076C
ORF Names:YOR29-27
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR076C.
SGDiS000005602. SKI7.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • mitochondrion Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 747747Superkiller protein 7PRO_0000269648Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei88 – 881PhosphoserineCombined sources
Modified residuei90 – 901PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08491.

PTM databases

iPTMnetiQ08491.

Interactioni

Subunit structurei

Interacts with the exosome and with the SKI complex composed of at least SKI2, SKI3 and SKI8. Interacts directly with SKI3 and SKI8.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CSL4P538595EBI-1389,EBI-1731
SKI3P178832EBI-1389,EBI-1861
SKI8Q027933EBI-1389,EBI-17260
UPF1Q929002EBI-1389,EBI-373471From a different organism.

GO - Molecular functioni

  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi34475. 105 interactions.
DIPiDIP-995N.
IntActiQ08491. 17 interactions.
MINTiMINT-518978.

Structurei

Secondary structure

1
747
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi258 – 2625Combined sources
Beta strandi266 – 2738Combined sources
Helixi280 – 29011Combined sources
Helixi296 – 30510Combined sources
Helixi306 – 3094Combined sources
Turni311 – 3133Combined sources
Helixi318 – 3214Combined sources
Helixi325 – 3306Combined sources
Beta strandi337 – 3415Combined sources
Beta strandi350 – 3567Combined sources
Helixi361 – 3644Combined sources
Turni365 – 3673Combined sources
Helixi368 – 3758Combined sources
Beta strandi378 – 3858Combined sources
Helixi390 – 3934Combined sources
Helixi401 – 4099Combined sources
Turni410 – 4156Combined sources
Beta strandi422 – 4276Combined sources
Helixi429 – 4324Combined sources
Helixi436 – 45217Combined sources
Helixi458 – 4603Combined sources
Beta strandi461 – 4655Combined sources
Turni468 – 4714Combined sources
Helixi504 – 51512Combined sources
Turni522 – 5243Combined sources
Beta strandi528 – 5369Combined sources
Beta strandi542 – 55817Combined sources
Beta strandi562 – 5665Combined sources
Beta strandi568 – 5714Combined sources
Beta strandi573 – 58311Combined sources
Beta strandi593 – 5953Combined sources
Beta strandi597 – 6004Combined sources
Beta strandi604 – 6129Combined sources
Turni617 – 6193Combined sources
Beta strandi629 – 6324Combined sources
Beta strandi648 – 66013Combined sources
Beta strandi673 – 6786Combined sources
Beta strandi681 – 69111Combined sources
Beta strandi696 – 6983Combined sources
Beta strandi703 – 7108Combined sources
Beta strandi717 – 7226Combined sources
Beta strandi728 – 7325Combined sources
Beta strandi738 – 7447Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4ZKDX-ray2.18A254-747[»]
4ZKEX-ray2.25A254-747[»]
5G06electron microscopy4.20P1-747[»]
ProteinModelPortaliQ08491.
SMRiQ08491. Positions 255-747.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini265 – 503239tr-type GPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni274 – 2818G1PROSITE-ProRule annotation
Regioni331 – 3355G2PROSITE-ProRule annotation
Regioni356 – 3594G3PROSITE-ProRule annotation
Regioni427 – 4304G4PROSITE-ProRule annotation
Regioni467 – 4693G5PROSITE-ProRule annotation

Domaini

The N-terminal domain (residues 1 to 264) is required and sufficient for interaction with the exosome and SKI complexes and for 3'-to-5' mRNA degradation.1 Publication

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00600000085333.
InParanoidiQ08491.
KOiK12595.
OMAiTERENGF.
OrthoDBiEOG092C0R0J.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08491-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLEQLARK RIEKSKGLLS ADQSHSTSKS ASLLERLHKN RETKDNNAET
60 70 80 90 100
KRKDLKTLLA KDKVKRSDFT PNQHSVSLSL KLSALKKSNS DLEKQGKSVT
110 120 130 140 150
LDSKENELPT KRKSPDDKLN LEESWKAIKE MNHYCFLKND PCINQTDDFA
160 170 180 190 200
FTNFIIKDKK NSLSTSIPLS SQNSSFLSLK KHNNELLGIF VPCNLPKTTR
210 220 230 240 250
KVAIENFNRP SPDDIIQSAQ LNAFNEKLEN LNIKSVPKAE KKEPINLQTP
260 270 280 290 300
PTESIDIHSF IATHPLNLTC LFLGDTNAGK STLLGHLLYD LNEISMSSMR
310 320 330 340 350
ELQKKSSNLD PSSSNSFKVI LDNTKTEREN GFSMFKKVIQ VENDLLPPSS
360 370 380 390 400
TLTLIDTPGS IKYFNKETLN SILTFDPEVY VLVIDCNYDS WEKSLDGPNN
410 420 430 440 450
QIYEILKVIS YLNKNSACKK HLIILLNKAD LISWDKHRLE MIQSELNYVL
460 470 480 490 500
KENFQWTDAE FQFIPCSGLL GSNLNKTENI TKSKYKSEFD SINYVPEWYE
510 520 530 540 550
GPTFFSQLYL LVEHNMNKIE TTLEEPFVGT ILQSSVLQPI AEINYVSLKV
560 570 580 590 600
LINSGYIQSG QTIEIHTQYE DFHYYGIVSR MKNSKQILET NTKNNISVGL
610 620 630 640 650
NPDILEVLVK IHNTEDFTKK QFHIRKGDII IHSRKTNTLS PNLPNTLKLL
660 670 680 690 700
ALRLIKLSIQ THALSDPVDL GSELLLYHNL THNAVKLVKI LGTNDISINP
710 720 730 740
NQSLIVEVEI IEPDFALNVI DSKYITNNIV LTSIDHKVIA VGRIACQ
Length:747
Mass (Da):84,779
Last modified:November 1, 1996 - v1
Checksum:iD93B956D02050BFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70678 Genomic DNA. Translation: CAA94561.1.
Z74984 Genomic DNA. Translation: CAA99269.1.
BK006948 Genomic DNA. Translation: DAA10855.1.
PIRiS66959.
RefSeqiNP_014719.1. NM_001183495.1.

Genome annotation databases

EnsemblFungiiYOR076C; YOR076C; YOR076C.
GeneIDi854243.
KEGGisce:YOR076C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70678 Genomic DNA. Translation: CAA94561.1.
Z74984 Genomic DNA. Translation: CAA99269.1.
BK006948 Genomic DNA. Translation: DAA10855.1.
PIRiS66959.
RefSeqiNP_014719.1. NM_001183495.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4ZKDX-ray2.18A254-747[»]
4ZKEX-ray2.25A254-747[»]
5G06electron microscopy4.20P1-747[»]
ProteinModelPortaliQ08491.
SMRiQ08491. Positions 255-747.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34475. 105 interactions.
DIPiDIP-995N.
IntActiQ08491. 17 interactions.
MINTiMINT-518978.

PTM databases

iPTMnetiQ08491.

Proteomic databases

MaxQBiQ08491.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR076C; YOR076C; YOR076C.
GeneIDi854243.
KEGGisce:YOR076C.

Organism-specific databases

EuPathDBiFungiDB:YOR076C.
SGDiS000005602. SKI7.

Phylogenomic databases

GeneTreeiENSGT00600000085333.
InParanoidiQ08491.
KOiK12595.
OMAiTERENGF.
OrthoDBiEOG092C0R0J.

Enzyme and pathway databases

BioCyciYEAST:G3O-33613-MONOMER.

Miscellaneous databases

PROiQ08491.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSKI7_YEAST
AccessioniPrimary (citable) accession number: Q08491
Secondary accession number(s): D6W2D9, O00032
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 233 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.