Superkiller protein 7 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q08491 (SKI7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 104. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Superkiller protein 7

Gene names

Name:	SKI7
Ordered Locus Names:	YOR076C
ORF Names:	YOR29-27

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	747 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Represses the expression of non-poly(A) mRNAs like L-A or M viruses and is therefore involved in antiviral system. Mediates interactions via its N-terminus between the exosome and the SKI complex which operate in the 3'-to-5' mRNA-decay pathway. By interacting with NAM7, is also required for nonsense-mediated 3'-to-5' mRNA-decay (NMD). May recognize a stalled 80S ribosome at the 3'-end of a nonstop mRNA which leads to the recruitment of the exosome and SKI complexes to the mRNAs to be degraded. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.15
Subunit structure	Interacts with the exosome and with the SKI complex composed of at least SKI2, SKI3 and SKI8. Interacts directly with SKI3 and SKI8. Ref.7 Ref.9 Ref.10 Ref.16 Ref.17
Subcellular location	Cytoplasm Ref.7 Ref.14.
Domain	The N-terminal domain (residues 1 to 264) is required and sufficient for interaction with the exosome and SKI complexes and for 3'-to-5' mRNA degradation. Ref.7
Miscellaneous	Present with 233 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Nonsense-mediated mRNA decay Protein biosynthesis Translation regulation
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC nonfunctional rRNA decay Inferred from mutant phenotype PubMed 19481524. Source: SGD nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay Inferred from mutant phenotype Ref.10. Source: SGD nuclear-transcribed mRNA catabolic process, non-stop decay Inferred from mutant phenotype Ref.9 PubMed 17660569. Source: SGD regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay Ref.7. Source: SGD
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro protein binding, bridging Inferred from mutant phenotype Ref.7. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CSL4	P53859	5	EBI-1389,EBI-1731
UPF1	Q92900	2	EBI-1389,EBI-373471	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 747

747

Superkiller protein 7

PRO_0000269648

Regions

Nucleotide binding

274 – 281

GTP By similarity

Nucleotide binding

356 – 360

GTP By similarity

Nucleotide binding

427 – 430

GTP By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.18 Ref.19

Modified residue

Phosphoserine Ref.18 Ref.19

Sequences

Sequence

Length

Mass (Da)

Tools

Q08491 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D93B956D02050BFA
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FASTA

747

84,779

        10         20         30         40         50         60 
MSLLEQLARK RIEKSKGLLS ADQSHSTSKS ASLLERLHKN RETKDNNAET KRKDLKTLLA 

        70         80         90        100        110        120 
KDKVKRSDFT PNQHSVSLSL KLSALKKSNS DLEKQGKSVT LDSKENELPT KRKSPDDKLN 

       130        140        150        160        170        180 
LEESWKAIKE MNHYCFLKND PCINQTDDFA FTNFIIKDKK NSLSTSIPLS SQNSSFLSLK 

       190        200        210        220        230        240 
KHNNELLGIF VPCNLPKTTR KVAIENFNRP SPDDIIQSAQ LNAFNEKLEN LNIKSVPKAE 

       250        260        270        280        290        300 
KKEPINLQTP PTESIDIHSF IATHPLNLTC LFLGDTNAGK STLLGHLLYD LNEISMSSMR 

       310        320        330        340        350        360 
ELQKKSSNLD PSSSNSFKVI LDNTKTEREN GFSMFKKVIQ VENDLLPPSS TLTLIDTPGS 

       370        380        390        400        410        420 
IKYFNKETLN SILTFDPEVY VLVIDCNYDS WEKSLDGPNN QIYEILKVIS YLNKNSACKK 

       430        440        450        460        470        480 
HLIILLNKAD LISWDKHRLE MIQSELNYVL KENFQWTDAE FQFIPCSGLL GSNLNKTENI 

       490        500        510        520        530        540 
TKSKYKSEFD SINYVPEWYE GPTFFSQLYL LVEHNMNKIE TTLEEPFVGT ILQSSVLQPI 

       550        560        570        580        590        600 
AEINYVSLKV LINSGYIQSG QTIEIHTQYE DFHYYGIVSR MKNSKQILET NTKNNISVGL 

       610        620        630        640        650        660 
NPDILEVLVK IHNTEDFTKK QFHIRKGDII IHSRKTNTLS PNLPNTLKLL ALRLIKLSIQ 

       670        680        690        700        710        720 
THALSDPVDL GSELLLYHNL THNAVKLVKI LGTNDISINP NQSLIVEVEI IEPDFALNVI 

       730        740 
DSKYITNNIV LTSIDHKVIA VGRIACQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames." Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M. Yeast 13:379-390(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Superkiller mutations in Saccharomyces cerevisiae suppress exclusion of M2 double-stranded RNA by L-A-HN and confer cold sensitivity in the presence of M and L-A-HN." Ridley S.P., Sommer S.S., Wickner R.B. Mol. Cell. Biol. 4:761-770(1984) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"The ski7 antiviral protein is an EF1-alpha homolog that blocks expression of non-Poly(A) mRNA in Saccharomyces cerevisiae." Benard L., Carroll K., Valle R.C.P., Masison D.C., Wickner R.B. J. Virol. 73:2893-2900(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Function of the ski4p (Csl4p) and Ski7p proteins in 3'-to-5' degradation of mRNA." van Hoof A., Staples R.R., Baker R.E., Parker R. Mol. Cell. Biol. 20:8230-8243(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Ski7p G protein interacts with the exosome and the Ski complex for 3'-to-5' mRNA decay in yeast." Araki Y., Takahashi S., Kobayashi T., Kajiho H., Hoshino S., Katada T. EMBO J. 20:4684-4693(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH THE EXOSOME AND SKI COMPLEXES.
[8]	"The yeast cytoplasmic LsmI/Pat1p complex protects mRNA 3' termini from partial degradation." He W., Parker R. Genetics 158:1445-1455(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Exosome-mediated recognition and degradation of mRNAs lacking a termination codon." van Hoof A., Frischmeyer P.A., Dietz H.C., Parker R. Science 295:2262-2264(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH THE EXOSOME COMPLEX.
[10]	"Interaction between Ski7p and Upf1p is required for nonsense-mediated 3'-to-5' mRNA decay in yeast." Takahashi S., Araki Y., Sakuno T., Katada T. EMBO J. 22:3951-3959(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH NAM7.
[11]	"An NMD pathway in yeast involving accelerated deadenylation and exosome-mediated 3'-->5' degradation." Mitchell P., Tollervey D. Mol. Cell 11:1405-1413(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[13]	"Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus." Kushner D.B., Lindenbach B.D., Grdzelishvili V.Z., Noueiry A.O., Paul S.M., Ahlquist P. Proc. Natl. Acad. Sci. U.S.A. 100:15764-15769(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[14]	"Decapping and decay of messenger RNA occur in cytoplasmic processing bodies." Sheth U., Parker R. Science 300:805-808(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[15]	"Translation of aberrant mRNAs lacking a termination codon or with a shortened 3'-UTR is repressed after initiation in yeast." Inada T., Aiba H. EMBO J. 24:1584-1595(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[16]	"Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p." Wang L., Lewis M.S., Johnson A.W. RNA 11:1291-1302(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SKI3 AND SKI8.
[17]	"A single subunit, Dis3, is essentially responsible for yeast exosome core activity." Dziembowski A., Lorentzen E., Conti E., Seraphin B. Nat. Struct. Mol. Biol. 14:15-22(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH THE EXOSOME.
[18]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-88 AND SER-90, MASS SPECTROMETRY.
[19]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-88 AND SER-90, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z70678 Genomic DNA. Translation: CAA94561.1. Z74984 Genomic DNA. Translation: CAA99269.1. BK006948 Genomic DNA. Translation: DAA10855.1.
PIR	S66959.
RefSeq	NP_014719.1. NM_001183495.1.
3D structure databases
ProteinModelPortal	Q08491.
SMR	Q08491. Positions 249-566.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-995N.
IntAct	Q08491. 16 interactions.
MINT	MINT-518978.
Proteomic databases
PaxDb	Q08491.
PeptideAtlas	Q08491.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR076C; YOR076C; YOR076C.
GeneID	854243.
KEGG	sce:YOR076C.
Organism-specific databases
CYGD	YOR076c.
SGD	S000005602. SKI7.
Phylogenomic databases
eggNOG	COG5256.
GeneTree	ENSGT00600000085333.
KO	K12595.
OMA	NSEANTE.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33613-MONOMER.
Gene expression databases
Genevestigator	Q08491.
Family and domain databases
InterPro	IPR000795. EF_GTP-bd_dom. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF00009. GTP_EFTU. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
ProtoNet	Search...
Other
NextBio	976147.

Entry information

Entry name

SKI7_YEAST

Accession

Primary (citable) accession number: Q08491
Secondary accession number(s): D6W2D9, O00032

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents