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Protein

Shugoshin

Gene

SGO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a central role in chromosome cohesion during mitosis and meiosis divisions by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. Probably act by protecting REC8 and RAD21 from separase degradation during anaphase. Also acts as a spindle checkpoint component required for sensing tension between sister chromatids during mitosis, its degradation when they separate preventing cell cycle arrest and chromosome loss in anaphase, a time when sister chromatids are no longer under tension.3 Publications

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. centromere complex assembly Source: SGD
  3. establishment of protein localization Source: SGD
  4. establishment of protein localization to chromosome Source: SGD
  5. kinetochore organization Source: SGD
  6. maintenance of meiotic sister chromatid cohesion Source: SGD
  7. meiotic sister chromatid segregation Source: SGD
  8. meiotic sister chromatid separation Source: SGD
  9. mitotic sister chromatid segregation Source: SGD
  10. mitotic spindle assembly checkpoint Source: SGD
  11. positive regulation of maintenance of meiotic sister chromatid cohesion Source: SGD
  12. sister chromatid biorientation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Meiosis, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-33611-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Shugoshin
Gene namesi
Name:SGO1
Ordered Locus Names:YOR073W
ORF Names:YOR29-24
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XV

Organism-specific databases

CYGDiYOR073w.
EuPathDBiFungiDB:YOR073W.
SGDiS000005599. SGO1.

Subcellular locationi

  1. Nucleus
  2. Chromosomecentromere
  3. Chromosomecentromerekinetochore
  4. Cytoplasmcytoskeletonspindle pole

  5. Note: Localizes to the centromere during S and M phases. Colocalizes with kinetochores. Associates with the spindle pole.

GO - Cellular componenti

  1. condensed nuclear chromosome, centromeric region Source: SGD
  2. condensed nuclear chromosome kinetochore Source: SGD
  3. cytoplasm Source: UniProtKB-KW
  4. spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi379 – 3791T → I in sgo1-100; induces a lack of response when chromosomes that are not under tension. 1 Publication
Mutagenesisi390 – 3901P → H in sgo1-700; induces a lack of response when chromosomes that are not under tension. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 590590ShugoshinPRO_0000055450Add
BLAST

Post-translational modificationi

Ubiquitinated by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation.Curated

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ08490.

Expressioni

Developmental stagei

Appears in S phase cells and remains present during mitosis until metaphase. Degraded at the onset of anaphase. During meiosis it is present until anaphase II and is then degraded. Not present in G1 cells.1 Publication

Gene expression databases

GenevestigatoriQ08490.

Interactioni

Protein-protein interaction databases

BioGridi34472. 115 interactions.
DIPiDIP-4161N.
MINTiMINT-491724.

Structurei

3D structure databases

ProteinModelPortaliQ08490.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili25 – 8662Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the shugoshin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG39343.
HOGENOMiHOG000154308.
InParanoidiQ08490.
KOiK11582.
OMAiKMRRPTE.
OrthoDBiEOG7P8PJN.

Family and domain databases

InterProiIPR011515. Shugoshin_C.
IPR011516. Shugoshin_N.
[Graphical view]
PfamiPF07557. Shugoshin_C. 1 hit.
PF07558. Shugoshin_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08490-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKRKIAPNK ESSRRTVSHD DLTPQIQEFQ NLMDLESQKV ENIRQSYSRQ
60 70 80 90 100
NSLLAKDNSI LKIKVNSLEK KISQLVQENV TLRSKTSISE AIYRERLSNQ
110 120 130 140 150
LQVIENGIIQ RFDEIFYMFE NVRKNENLPS SSLRTMLKRT SSRSRSCSLS
160 170 180 190 200
SPTYSKSYTR LSNHENNLSH ESSFNKDDGP DLEPKAKKRK SSRRQSMFVS
210 220 230 240 250
TSLEPEDETG ENEPMMENSS VEVPAESHES AQVEETIDAL NPEEENSDSV
260 270 280 290 300
SNFTNSIIEY SIPEENPTEP EHSSSKLEIF NDSTNMLSTV PSNPLPLPLP
310 320 330 340 350
GPSATLPTTT SDASTVYPSS SSSTNSHPKT KIKHSMKPPR IELKKKVIDE
360 370 380 390 400
VMPVSNMSSN SEISFTRTRR TRGKAVDYTL PSLRAKMRRP SEKLVDATTV
410 420 430 440 450
IDIHDLQVSK RNRETSHKRK SLSQDSIPDE PQLREVVVSK DYGTPKGKKT
460 470 480 490 500
EDEIHEDTAH LMTTSNNNSN NKNEKKLTSN NSPKKSSPLL DITNKSENKK
510 520 530 540 550
KSTRTKKLFK NAIVNNLSDE NSTTRPSKSS KGTSNNNNNY NNFDNNNSNI
560 570 580 590
NNVNNKSVSF RLNEDDLAVF DLFGNGKAVK HQPKTYRTKK
Length:590
Mass (Da):66,706
Last modified:November 1, 1996 - v1
Checksum:iEBC61416CD8E4263
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74981 Genomic DNA. Translation: CAA99266.1.
Z70678 Genomic DNA. Translation: CAA94558.1.
BK006948 Genomic DNA. Translation: DAA10852.1.
PIRiS66956.
RefSeqiNP_014716.1. NM_001183492.1.

Genome annotation databases

EnsemblFungiiYOR073W; YOR073W; YOR073W.
GeneIDi854240.
KEGGisce:YOR073W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74981 Genomic DNA. Translation: CAA99266.1.
Z70678 Genomic DNA. Translation: CAA94558.1.
BK006948 Genomic DNA. Translation: DAA10852.1.
PIRiS66956.
RefSeqiNP_014716.1. NM_001183492.1.

3D structure databases

ProteinModelPortaliQ08490.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34472. 115 interactions.
DIPiDIP-4161N.
MINTiMINT-491724.

Proteomic databases

PaxDbiQ08490.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR073W; YOR073W; YOR073W.
GeneIDi854240.
KEGGisce:YOR073W.

Organism-specific databases

CYGDiYOR073w.
EuPathDBiFungiDB:YOR073W.
SGDiS000005599. SGO1.

Phylogenomic databases

eggNOGiNOG39343.
HOGENOMiHOG000154308.
InParanoidiQ08490.
KOiK11582.
OMAiKMRRPTE.
OrthoDBiEOG7P8PJN.

Enzyme and pathway databases

BioCyciYEAST:G3O-33611-MONOMER.

Miscellaneous databases

NextBioi976138.
PROiQ08490.

Gene expression databases

GenevestigatoriQ08490.

Family and domain databases

InterProiIPR011515. Shugoshin_C.
IPR011516. Shugoshin_N.
[Graphical view]
PfamiPF07557. Shugoshin_C. 1 hit.
PF07558. Shugoshin_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames."
    Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M.
    Yeast 13:379-390(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. "Maintenance of cohesin at centromeres after meiosis I in budding yeast requires a kinetochore-associated protein related to MEI-S332."
    Katis V.L., Galova M., Rabitsch K.P., Gregan J., Nasmyth K.
    Curr. Biol. 14:560-572(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "A genome-wide screen identifies genes required for centromeric cohesion."
    Marston A.L., Tham W.-H., Shah H., Amon A.
    Science 303:1367-1370(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, PROBABLE UBIQUITINATION.
  7. "Mnd2, an essential antagonist of the anaphase-promoting complex during meiotic prophase."
    Penkner A.M., Prinz S., Ferscha S., Klein F.
    Cell 120:789-801(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEGRADATION.
  8. "The centromeric protein Sgo1 is required to sense lack of tension on mitotic chromosomes."
    Indjeian V.B., Stern B.M., Murray A.W.
    Science 307:130-133(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PROBABLE UBIQUITINATION, MUTAGENESIS OF THR-379 AND PRO-390.

Entry informationi

Entry nameiSGO1_YEAST
AccessioniPrimary (citable) accession number: Q08490
Secondary accession number(s): D6W2D6, O00031
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: November 1, 1996
Last modified: April 29, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.