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Protein

Shugoshin

Gene

SGO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a central role in chromosome cohesion during mitosis and meiosis divisions by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. Probably act by protecting REC8 and RAD21 from separase degradation during anaphase. Also acts as a spindle checkpoint component required for sensing tension between sister chromatids during mitosis, its degradation when they separate preventing cell cycle arrest and chromosome loss in anaphase, a time when sister chromatids are no longer under tension.3 Publications

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • centromere complex assembly Source: SGD
  • establishment of protein localization Source: SGD
  • establishment of protein localization to chromosome Source: SGD
  • kinetochore organization Source: SGD
  • maintenance of meiotic sister chromatid cohesion Source: SGD
  • meiotic sister chromatid segregation Source: SGD
  • meiotic sister chromatid separation Source: SGD
  • mitotic sister chromatid segregation Source: SGD
  • mitotic spindle assembly checkpoint Source: SGD
  • positive regulation of maintenance of meiotic sister chromatid cohesion Source: SGD
  • sister chromatid biorientation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Meiosis, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-33611-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Shugoshin
Gene namesi
Name:SGO1
Ordered Locus Names:YOR073W
ORF Names:YOR29-24
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR073W.
SGDiS000005599. SGO1.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome, centromeric region Source: SGD
  • condensed nuclear chromosome kinetochore Source: SGD
  • cytoplasm Source: UniProtKB-KW
  • spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi379T → I in sgo1-100; induces a lack of response when chromosomes that are not under tension. 1 Publication1
Mutagenesisi390P → H in sgo1-700; induces a lack of response when chromosomes that are not under tension. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000554501 – 590ShugoshinAdd BLAST590

Post-translational modificationi

Ubiquitinated by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation.Curated

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiQ08490.

Expressioni

Developmental stagei

Appears in S phase cells and remains present during mitosis until metaphase. Degraded at the onset of anaphase. During meiosis it is present until anaphase II and is then degraded. Not present in G1 cells.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi34472. 121 interactors.
DIPiDIP-4161N.
MINTiMINT-491724.

Structurei

3D structure databases

ProteinModelPortaliQ08490.
SMRiQ08490.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili25 – 86Sequence analysisAdd BLAST62

Sequence similaritiesi

Belongs to the shugoshin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000154308.
InParanoidiQ08490.
KOiK11582.
OMAiQSKIKHS.
OrthoDBiEOG092C335D.

Family and domain databases

InterProiIPR011515. Shugoshin_C.
IPR011516. Shugoshin_N.
[Graphical view]
PfamiPF07557. Shugoshin_C. 1 hit.
PF07558. Shugoshin_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08490-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKRKIAPNK ESSRRTVSHD DLTPQIQEFQ NLMDLESQKV ENIRQSYSRQ
60 70 80 90 100
NSLLAKDNSI LKIKVNSLEK KISQLVQENV TLRSKTSISE AIYRERLSNQ
110 120 130 140 150
LQVIENGIIQ RFDEIFYMFE NVRKNENLPS SSLRTMLKRT SSRSRSCSLS
160 170 180 190 200
SPTYSKSYTR LSNHENNLSH ESSFNKDDGP DLEPKAKKRK SSRRQSMFVS
210 220 230 240 250
TSLEPEDETG ENEPMMENSS VEVPAESHES AQVEETIDAL NPEEENSDSV
260 270 280 290 300
SNFTNSIIEY SIPEENPTEP EHSSSKLEIF NDSTNMLSTV PSNPLPLPLP
310 320 330 340 350
GPSATLPTTT SDASTVYPSS SSSTNSHPKT KIKHSMKPPR IELKKKVIDE
360 370 380 390 400
VMPVSNMSSN SEISFTRTRR TRGKAVDYTL PSLRAKMRRP SEKLVDATTV
410 420 430 440 450
IDIHDLQVSK RNRETSHKRK SLSQDSIPDE PQLREVVVSK DYGTPKGKKT
460 470 480 490 500
EDEIHEDTAH LMTTSNNNSN NKNEKKLTSN NSPKKSSPLL DITNKSENKK
510 520 530 540 550
KSTRTKKLFK NAIVNNLSDE NSTTRPSKSS KGTSNNNNNY NNFDNNNSNI
560 570 580 590
NNVNNKSVSF RLNEDDLAVF DLFGNGKAVK HQPKTYRTKK
Length:590
Mass (Da):66,706
Last modified:November 1, 1996 - v1
Checksum:iEBC61416CD8E4263
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74981 Genomic DNA. Translation: CAA99266.1.
Z70678 Genomic DNA. Translation: CAA94558.1.
BK006948 Genomic DNA. Translation: DAA10852.1.
PIRiS66956.
RefSeqiNP_014716.1. NM_001183492.1.

Genome annotation databases

EnsemblFungiiYOR073W; YOR073W; YOR073W.
GeneIDi854240.
KEGGisce:YOR073W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74981 Genomic DNA. Translation: CAA99266.1.
Z70678 Genomic DNA. Translation: CAA94558.1.
BK006948 Genomic DNA. Translation: DAA10852.1.
PIRiS66956.
RefSeqiNP_014716.1. NM_001183492.1.

3D structure databases

ProteinModelPortaliQ08490.
SMRiQ08490.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34472. 121 interactors.
DIPiDIP-4161N.
MINTiMINT-491724.

Proteomic databases

PRIDEiQ08490.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR073W; YOR073W; YOR073W.
GeneIDi854240.
KEGGisce:YOR073W.

Organism-specific databases

EuPathDBiFungiDB:YOR073W.
SGDiS000005599. SGO1.

Phylogenomic databases

HOGENOMiHOG000154308.
InParanoidiQ08490.
KOiK11582.
OMAiQSKIKHS.
OrthoDBiEOG092C335D.

Enzyme and pathway databases

BioCyciYEAST:G3O-33611-MONOMER.

Miscellaneous databases

PROiQ08490.

Family and domain databases

InterProiIPR011515. Shugoshin_C.
IPR011516. Shugoshin_N.
[Graphical view]
PfamiPF07557. Shugoshin_C. 1 hit.
PF07558. Shugoshin_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSGO1_YEAST
AccessioniPrimary (citable) accession number: Q08490
Secondary accession number(s): D6W2D6, O00031
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.