Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q08490

- SGO1_YEAST

UniProt

Q08490 - SGO1_YEAST

Protein

Shugoshin

Gene

SGO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Plays a central role in chromosome cohesion during mitosis and meiosis divisions by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. Probably act by protecting REC8 and RAD21 from separase degradation during anaphase. Also acts as a spindle checkpoint component required for sensing tension between sister chromatids during mitosis, its degradation when they separate preventing cell cycle arrest and chromosome loss in anaphase, a time when sister chromatids are no longer under tension.3 Publications

    GO - Biological processi

    1. centromere complex assembly Source: SGD
    2. kinetochore organization Source: SGD
    3. maintenance of meiotic sister chromatid cohesion Source: SGD
    4. meiotic sister chromatid segregation Source: SGD
    5. meiotic sister chromatid separation Source: SGD
    6. mitotic sister chromatid segregation Source: SGD
    7. mitotic spindle assembly checkpoint Source: SGD
    8. positive regulation of maintenance of meiotic sister chromatid cohesion Source: SGD
    9. sister chromatid biorientation Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Chromosome partition, Meiosis, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33611-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Shugoshin
    Gene namesi
    Name:SGO1
    Ordered Locus Names:YOR073W
    ORF Names:YOR29-24
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR073w.
    SGDiS000005599. SGO1.

    Subcellular locationi

    Nucleus. Chromosomecentromere. Chromosomecentromerekinetochore. Cytoplasmcytoskeletonspindle pole
    Note: Localizes to the centromere during S and M phases. Colocalizes with kinetochores. Associates with the spindle pole.

    GO - Cellular componenti

    1. condensed nuclear chromosome, centromeric region Source: SGD
    2. condensed nuclear chromosome kinetochore Source: SGD
    3. cytoplasm Source: UniProtKB-KW
    4. spindle pole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi379 – 3791T → I in sgo1-100; induces a lack of response when chromosomes that are not under tension. 1 Publication
    Mutagenesisi390 – 3901P → H in sgo1-700; induces a lack of response when chromosomes that are not under tension. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 590590ShugoshinPRO_0000055450Add
    BLAST

    Post-translational modificationi

    Ubiquitinated by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation.Curated

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PaxDbiQ08490.

    Expressioni

    Developmental stagei

    Appears in S phase cells and remains present during mitosis until metaphase. Degraded at the onset of anaphase. During meiosis it is present until anaphase II and is then degraded. Not present in G1 cells.1 Publication

    Gene expression databases

    GenevestigatoriQ08490.

    Interactioni

    Protein-protein interaction databases

    BioGridi34472. 115 interactions.
    DIPiDIP-4161N.
    MINTiMINT-491724.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08490.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili25 – 8662Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the shugoshin family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG39343.
    HOGENOMiHOG000154308.
    KOiK11582.
    OMAiTTVTDIH.
    OrthoDBiEOG7P8PJN.

    Family and domain databases

    InterProiIPR011515. Shugoshin_C.
    IPR011516. Shugoshin_N.
    [Graphical view]
    PfamiPF07557. Shugoshin_C. 1 hit.
    PF07558. Shugoshin_N. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q08490-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPKRKIAPNK ESSRRTVSHD DLTPQIQEFQ NLMDLESQKV ENIRQSYSRQ    50
    NSLLAKDNSI LKIKVNSLEK KISQLVQENV TLRSKTSISE AIYRERLSNQ 100
    LQVIENGIIQ RFDEIFYMFE NVRKNENLPS SSLRTMLKRT SSRSRSCSLS 150
    SPTYSKSYTR LSNHENNLSH ESSFNKDDGP DLEPKAKKRK SSRRQSMFVS 200
    TSLEPEDETG ENEPMMENSS VEVPAESHES AQVEETIDAL NPEEENSDSV 250
    SNFTNSIIEY SIPEENPTEP EHSSSKLEIF NDSTNMLSTV PSNPLPLPLP 300
    GPSATLPTTT SDASTVYPSS SSSTNSHPKT KIKHSMKPPR IELKKKVIDE 350
    VMPVSNMSSN SEISFTRTRR TRGKAVDYTL PSLRAKMRRP SEKLVDATTV 400
    IDIHDLQVSK RNRETSHKRK SLSQDSIPDE PQLREVVVSK DYGTPKGKKT 450
    EDEIHEDTAH LMTTSNNNSN NKNEKKLTSN NSPKKSSPLL DITNKSENKK 500
    KSTRTKKLFK NAIVNNLSDE NSTTRPSKSS KGTSNNNNNY NNFDNNNSNI 550
    NNVNNKSVSF RLNEDDLAVF DLFGNGKAVK HQPKTYRTKK 590
    Length:590
    Mass (Da):66,706
    Last modified:November 1, 1996 - v1
    Checksum:iEBC61416CD8E4263
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z74981 Genomic DNA. Translation: CAA99266.1.
    Z70678 Genomic DNA. Translation: CAA94558.1.
    BK006948 Genomic DNA. Translation: DAA10852.1.
    PIRiS66956.
    RefSeqiNP_014716.1. NM_001183492.1.

    Genome annotation databases

    EnsemblFungiiYOR073W; YOR073W; YOR073W.
    GeneIDi854240.
    KEGGisce:YOR073W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z74981 Genomic DNA. Translation: CAA99266.1 .
    Z70678 Genomic DNA. Translation: CAA94558.1 .
    BK006948 Genomic DNA. Translation: DAA10852.1 .
    PIRi S66956.
    RefSeqi NP_014716.1. NM_001183492.1.

    3D structure databases

    ProteinModelPortali Q08490.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34472. 115 interactions.
    DIPi DIP-4161N.
    MINTi MINT-491724.

    Proteomic databases

    PaxDbi Q08490.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR073W ; YOR073W ; YOR073W .
    GeneIDi 854240.
    KEGGi sce:YOR073W.

    Organism-specific databases

    CYGDi YOR073w.
    SGDi S000005599. SGO1.

    Phylogenomic databases

    eggNOGi NOG39343.
    HOGENOMi HOG000154308.
    KOi K11582.
    OMAi TTVTDIH.
    OrthoDBi EOG7P8PJN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33611-MONOMER.

    Miscellaneous databases

    NextBioi 976138.

    Gene expression databases

    Genevestigatori Q08490.

    Family and domain databases

    InterProi IPR011515. Shugoshin_C.
    IPR011516. Shugoshin_N.
    [Graphical view ]
    Pfami PF07557. Shugoshin_C. 1 hit.
    PF07558. Shugoshin_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames."
      Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M.
      Yeast 13:379-390(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. "Maintenance of cohesin at centromeres after meiosis I in budding yeast requires a kinetochore-associated protein related to MEI-S332."
      Katis V.L., Galova M., Rabitsch K.P., Gregan J., Nasmyth K.
      Curr. Biol. 14:560-572(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. "A genome-wide screen identifies genes required for centromeric cohesion."
      Marston A.L., Tham W.-H., Shah H., Amon A.
      Science 303:1367-1370(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, PROBABLE UBIQUITINATION.
    7. "Mnd2, an essential antagonist of the anaphase-promoting complex during meiotic prophase."
      Penkner A.M., Prinz S., Ferscha S., Klein F.
      Cell 120:789-801(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEGRADATION.
    8. "The centromeric protein Sgo1 is required to sense lack of tension on mitotic chromosomes."
      Indjeian V.B., Stern B.M., Murray A.W.
      Science 307:130-133(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PROBABLE UBIQUITINATION, MUTAGENESIS OF THR-379 AND PRO-390.

    Entry informationi

    Entry nameiSGO1_YEAST
    AccessioniPrimary (citable) accession number: Q08490
    Secondary accession number(s): D6W2D6, O00031
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3