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Q08490 (SGO1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Shugoshin
Gene names
Name:SGO1
Ordered Locus Names:YOR073W
ORF Names:YOR29-24
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length590 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a central role in chromosome cohesion during mitosis and meiosis divisions by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. Probably act by protecting REC8 and RAD21 from separase degradation during anaphase. Also acts as a spindle checkpoint component required for sensing tension between sister chromatids during mitosis, its degradation when they separate preventing cell cycle arrest and chromosome loss in anaphase, a time when sister chromatids are no longer under tension. Ref.5 Ref.6 Ref.8

Subcellular location

Nucleus. Chromosomecentromere. Chromosomecentromerekinetochore. Cytoplasmcytoskeletonspindle pole. Note: Localizes to the centromere during S and M phases. Colocalizes with kinetochores. Associates with the spindle pole. Ref.4 Ref.5 Ref.6

Developmental stage

Appears in S phase cells and remains present during mitosis until metaphase. Degraded at the onset of anaphase. During meiosis it is present until anaphase II and is then degraded. Not present in G1 cells. Ref.6

Post-translational modification

Ubiquitinated by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation Probable. Ref.6 Ref.7 Ref.8

Sequence similarities

Belongs to the shugoshin family.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Chromosome partition
Meiosis
Mitosis
   Cellular componentCentromere
Chromosome
Cytoplasm
Cytoskeleton
Kinetochore
Nucleus
   DomainCoiled coil
   PTMUbl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcentromere complex assembly

Inferred from mutant phenotype PubMed 22365852. Source: SGD

kinetochore organization

Inferred from direct assay PubMed 22365852. Source: SGD

maintenance of meiotic sister chromatid cohesion

Inferred from mutant phenotype Ref.6. Source: SGD

meiotic sister chromatid segregation

Inferred from mutant phenotype PubMed 14730319Ref.5. Source: SGD

meiotic sister chromatid separation

Inferred from mutant phenotype Ref.5. Source: SGD

mitotic sister chromatid segregation

Inferred from mutant phenotype PubMed 14730319Ref.5Ref.8. Source: SGD

mitotic spindle assembly checkpoint

Inferred from mutant phenotype Ref.8. Source: SGD

positive regulation of maintenance of meiotic sister chromatid cohesion

Inferred from mutant phenotype Ref.5. Source: SGD

sister chromatid biorientation

Inferred from mutant phenotype PubMed 17980598PubMed 18081426. Source: SGD

   Cellular_componentcondensed nuclear chromosome kinetochore

Inferred from direct assay PubMed 14730319Ref.5. Source: SGD

condensed nuclear chromosome, centromeric region

Inferred from direct assay PubMed 14730319PubMed 16357219. Source: SGD

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

spindle pole

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 590590Shugoshin
PRO_0000055450

Regions

Coiled coil25 – 8662 Potential

Experimental info

Mutagenesis3791T → I in sgo1-100; induces a lack of response when chromosomes that are not under tension. Ref.8
Mutagenesis3901P → H in sgo1-700; induces a lack of response when chromosomes that are not under tension. Ref.8

Sequences

Sequence LengthMass (Da)Tools
Q08490 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: EBC61416CD8E4263

FASTA59066,706
        10         20         30         40         50         60 
MPKRKIAPNK ESSRRTVSHD DLTPQIQEFQ NLMDLESQKV ENIRQSYSRQ NSLLAKDNSI 

        70         80         90        100        110        120 
LKIKVNSLEK KISQLVQENV TLRSKTSISE AIYRERLSNQ LQVIENGIIQ RFDEIFYMFE 

       130        140        150        160        170        180 
NVRKNENLPS SSLRTMLKRT SSRSRSCSLS SPTYSKSYTR LSNHENNLSH ESSFNKDDGP 

       190        200        210        220        230        240 
DLEPKAKKRK SSRRQSMFVS TSLEPEDETG ENEPMMENSS VEVPAESHES AQVEETIDAL 

       250        260        270        280        290        300 
NPEEENSDSV SNFTNSIIEY SIPEENPTEP EHSSSKLEIF NDSTNMLSTV PSNPLPLPLP 

       310        320        330        340        350        360 
GPSATLPTTT SDASTVYPSS SSSTNSHPKT KIKHSMKPPR IELKKKVIDE VMPVSNMSSN 

       370        380        390        400        410        420 
SEISFTRTRR TRGKAVDYTL PSLRAKMRRP SEKLVDATTV IDIHDLQVSK RNRETSHKRK 

       430        440        450        460        470        480 
SLSQDSIPDE PQLREVVVSK DYGTPKGKKT EDEIHEDTAH LMTTSNNNSN NKNEKKLTSN 

       490        500        510        520        530        540 
NSPKKSSPLL DITNKSENKK KSTRTKKLFK NAIVNNLSDE NSTTRPSKSS KGTSNNNNNY 

       550        560        570        580        590 
NNFDNNNSNI NNVNNKSVSF RLNEDDLAVF DLFGNGKAVK HQPKTYRTKK 

« Hide

References

« Hide 'large scale' references
[1]"The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames."
Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M.
Yeast 13:379-390(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Maintenance of cohesin at centromeres after meiosis I in budding yeast requires a kinetochore-associated protein related to MEI-S332."
Katis V.L., Galova M., Rabitsch K.P., Gregan J., Nasmyth K.
Curr. Biol. 14:560-572(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"A genome-wide screen identifies genes required for centromeric cohesion."
Marston A.L., Tham W.-H., Shah H., Amon A.
Science 303:1367-1370(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, PROBABLE UBIQUITINATION.
[7]"Mnd2, an essential antagonist of the anaphase-promoting complex during meiotic prophase."
Penkner A.M., Prinz S., Ferscha S., Klein F.
Cell 120:789-801(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: DEGRADATION.
[8]"The centromeric protein Sgo1 is required to sense lack of tension on mitotic chromosomes."
Indjeian V.B., Stern B.M., Murray A.W.
Science 307:130-133(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PROBABLE UBIQUITINATION, MUTAGENESIS OF THR-379 AND PRO-390.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z74981 Genomic DNA. Translation: CAA99266.1.
Z70678 Genomic DNA. Translation: CAA94558.1.
BK006948 Genomic DNA. Translation: DAA10852.1.
PIRS66956.
RefSeqNP_014716.1. NM_001183492.1.

3D structure databases

ProteinModelPortalQ08490.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34472. 115 interactions.
DIPDIP-4161N.
MINTMINT-491724.

Proteomic databases

PaxDbQ08490.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR073W; YOR073W; YOR073W.
GeneID854240.
KEGGsce:YOR073W.

Organism-specific databases

CYGDYOR073w.
SGDS000005599. SGO1.

Phylogenomic databases

eggNOGNOG39343.
HOGENOMHOG000154308.
KOK11582.
OMATTVTDIH.
OrthoDBEOG7P8PJN.

Enzyme and pathway databases

BioCycYEAST:G3O-33611-MONOMER.

Gene expression databases

GenevestigatorQ08490.

Family and domain databases

InterProIPR011515. Shugoshin_C.
IPR011516. Shugoshin_N.
[Graphical view]
PfamPF07557. Shugoshin_C. 1 hit.
PF07558. Shugoshin_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio976138.

Entry information

Entry nameSGO1_YEAST
AccessionPrimary (citable) accession number: Q08490
Secondary accession number(s): D6W2D6, O00031
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families