Q08490 (SGO1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shugoshin

Gene names

Name:	SGO1
Ordered Locus Names:	YOR073W
ORF Names:	YOR29-24

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a central role in chromosome cohesion during mitosis and meiosis divisions by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. Probably act by protecting REC8 and RAD21 from separase degradation during anaphase. Also acts as a spindle checkpoint component required for sensing tension between sister chromatids during mitosis, its degradation when they separate preventing cell cycle arrest and chromosome loss in anaphase, a time when sister chromatids are no longer under tension. Ref.5 Ref.6 Ref.8
Subcellular location	Nucleus. Chromosome › centromere. Chromosome › centromere › kinetochore. Cytoplasm › cytoskeleton › spindle pole. Note: Localizes to the centromere during S and M phases. Colocalizes with kinetochores. Associates with the spindle pole. Ref.4 Ref.5 Ref.6
Developmental stage	Appears in S phase cells and remains present during mitosis until metaphase. Degraded at the onset of anaphase. During meiosis it is present until anaphase II and is then degraded. Not present in G1 cells. Ref.6
Post-translational modification	Ubiquitinated by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation Probable. Ref.6 Ref.7 Ref.8
Sequence similarities	Belongs to the shugoshin family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Chromosome partition Meiosis Mitosis
Cellular component	Centromere Chromosome Cytoplasm Cytoskeleton Kinetochore Nucleus
Domain	Coiled coil
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW maintenance of meiotic sister chromatid cohesion Inferred from mutant phenotype Ref.6. Source: SGD meiotic sister chromatid segregation Inferred from mutant phenotype PubMed 14730319Ref.5. Source: SGD meiotic sister chromatid separation Inferred from mutant phenotype Ref.5. Source: SGD mitotic spindle assembly checkpoint Inferred from mutant phenotype Ref.8. Source: SGD positive regulation of maintenance of meiotic sister chromatid cohesion Inferred from mutant phenotype Ref.5. Source: SGD sister chromatid biorientation Inferred from mutant phenotype PubMed 17980598PubMed 18081426. Source: SGD
Cellular_component	condensed nuclear chromosome kinetochore Inferred from direct assay PubMed 14730319Ref.5. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW spindle pole Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	Shugoshin		PRO_0000055450
Regions
	Coiled coil	25 – 86	62	Potential
Experimental info
	Mutagenesis	379	1	T → I in sgo1-100; induces a lack of response when chromosomes that are not under tension. Ref.8
	Mutagenesis	390	1	P → H in sgo1-700; induces a lack of response when chromosomes that are not under tension. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q08490 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: EBC61416CD8E4263	FASTA	590	66,706
	10 20 30 40 50 60 MPKRKIAPNK ESSRRTVSHD DLTPQIQEFQ NLMDLESQKV ENIRQSYSRQ NSLLAKDNSI 70 80 90 100 110 120 LKIKVNSLEK KISQLVQENV TLRSKTSISE AIYRERLSNQ LQVIENGIIQ RFDEIFYMFE 130 140 150 160 170 180 NVRKNENLPS SSLRTMLKRT SSRSRSCSLS SPTYSKSYTR LSNHENNLSH ESSFNKDDGP 190 200 210 220 230 240 DLEPKAKKRK SSRRQSMFVS TSLEPEDETG ENEPMMENSS VEVPAESHES AQVEETIDAL 250 260 270 280 290 300 NPEEENSDSV SNFTNSIIEY SIPEENPTEP EHSSSKLEIF NDSTNMLSTV PSNPLPLPLP 310 320 330 340 350 360 GPSATLPTTT SDASTVYPSS SSSTNSHPKT KIKHSMKPPR IELKKKVIDE VMPVSNMSSN 370 380 390 400 410 420 SEISFTRTRR TRGKAVDYTL PSLRAKMRRP SEKLVDATTV IDIHDLQVSK RNRETSHKRK 430 440 450 460 470 480 SLSQDSIPDE PQLREVVVSK DYGTPKGKKT EDEIHEDTAH LMTTSNNNSN NKNEKKLTSN 490 500 510 520 530 540 NSPKKSSPLL DITNKSENKK KSTRTKKLFK NAIVNNLSDE NSTTRPSKSS KGTSNNNNNY 550 560 570 580 590 NNFDNNNSNI NNVNNKSVSF RLNEDDLAVF DLFGNGKAVK HQPKTYRTKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames." Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M. Yeast 13:379-390(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Maintenance of cohesin at centromeres after meiosis I in budding yeast requires a kinetochore-associated protein related to MEI-S332." Katis V.L., Galova M., Rabitsch K.P., Gregan J., Nasmyth K. Curr. Biol. 14:560-572(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"A genome-wide screen identifies genes required for centromeric cohesion." Marston A.L., Tham W.-H., Shah H., Amon A. Science 303:1367-1370(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, PROBABLE UBIQUITINATION.
[7]	"Mnd2, an essential antagonist of the anaphase-promoting complex during meiotic prophase." Penkner A.M., Prinz S., Ferscha S., Klein F. Cell 120:789-801(2005) [PubMed] [Europe PMC] [Abstract] Cited for: DEGRADATION.
[8]	"The centromeric protein Sgo1 is required to sense lack of tension on mitotic chromosomes." Indjeian V.B., Stern B.M., Murray A.W. Science 307:130-133(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PROBABLE UBIQUITINATION, MUTAGENESIS OF THR-379 AND PRO-390.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z74981 Genomic DNA. Translation: CAA99266.1. Z70678 Genomic DNA. Translation: CAA94558.1. BK006948 Genomic DNA. Translation: DAA10852.1.
PIR	S66956.
RefSeq	NP_014716.1. NM_001183492.1.
3D structure databases
ProteinModelPortal	Q08490.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4161N.
IntAct	Q08490. 1 interaction.
MINT	MINT-491724.
Proteomic databases
PaxDb	Q08490.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR073W; YOR073W; YOR073W.
GeneID	854240.
KEGG	sce:YOR073W.
Organism-specific databases
CYGD	YOR073w.
SGD	S000005599. SGO1.
Phylogenomic databases
eggNOG	NOG39343.
HOGENOM	HOG000154308.
KO	K11582.
OMA	RRPTEKL.
OrthoDB	EOG49W5PQ.
Gene expression databases
Genevestigator	Q08490.
GermOnline	YOR073W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011515. Shugoshin_C. IPR011516. Shugoshin_N. [Graphical view]
Pfam	PF07557. Shugoshin_C. 1 hit. PF07558. Shugoshin_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976138.

Entry information

Entry name

SGO1_YEAST

Accession

Primary (citable) accession number: Q08490
Secondary accession number(s): D6W2D6, O00031

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program