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Protein

Nicotinamide riboside transporter 1

Gene

NRT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

High-affinity pH-dependent nicotinamide riboside transporter which also transports thiamine with low affinity. Involved in 5-fluorocytosine sensitivity.6 Publications

Kineticsi

  1. KM=21 µM for nicotinamide riboside1 Publication

    pH dependencei

    Optimum pH is 3.5-6.5.1 Publication

    GO - Molecular functioni

    • 5-amino-1-ribofuranosylimidazole-4-carboxamide transmembrane transporter activity Source: SGD
    • nicotinamide riboside transmembrane transporter activity Source: SGD
    • nucleobase transmembrane transporter activity Source: GO_Central

    GO - Biological processi

    • 5-amino-1-ribofuranosylimidazole-4-carboxamide transmembrane transport Source: SGD
    • nicotinamide riboside transport Source: SGD
    • nucleobase transport Source: GO_Central
    • thiamine transport Source: SGD

    Keywordsi

    Biological processTransport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33610-MONOMER

    Protein family/group databases

    TCDBi2.A.39.4.2 the nucleobase:cation symporter-1 (ncs1) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nicotinamide riboside transporter 1
    Alternative name(s):
    Thiamine transport protein 71
    Gene namesi
    Name:NRT1
    Synonyms:THI71
    Ordered Locus Names:YOR071C
    ORF Names:YOR29-22
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XV

    Organism-specific databases

    EuPathDBiFungiDB:YOR071C
    SGDiS000005597 NRT1

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei48 – 68HelicalSequence analysisAdd BLAST21
    Transmembranei71 – 91HelicalSequence analysisAdd BLAST21
    Transmembranei112 – 132HelicalSequence analysisAdd BLAST21
    Transmembranei174 – 194HelicalSequence analysisAdd BLAST21
    Transmembranei197 – 217HelicalSequence analysisAdd BLAST21
    Transmembranei241 – 261HelicalSequence analysisAdd BLAST21
    Transmembranei273 – 293HelicalSequence analysisAdd BLAST21
    Transmembranei372 – 392HelicalSequence analysisAdd BLAST21
    Transmembranei395 – 415HelicalSequence analysisAdd BLAST21
    Transmembranei447 – 467HelicalSequence analysisAdd BLAST21
    Transmembranei484 – 504HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001979271 – 598Nicotinamide riboside transporter 1Add BLAST598

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei560PhosphoserineCombined sources1
    Modified residuei572PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ08485
    PaxDbiQ08485
    PRIDEiQ08485

    PTM databases

    iPTMnetiQ08485

    Expressioni

    Inductioni

    Expression is controlled by the transcription repressors SUM1, HST1 and RFM1.1 Publication

    Interactioni

    Protein-protein interaction databases

    BioGridi34470, 60 interactors
    DIPiDIP-7934N
    IntActiQ08485, 1 interactor
    MINTiQ08485
    STRINGi4932.YOR071C

    Structurei

    3D structure databases

    ProteinModelPortaliQ08485
    SMRiQ08485
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00530000065049
    InParanoidiQ08485
    OMAiWYGSQAW
    OrthoDBiEOG092C1JZY

    Family and domain databases

    Gene3Di1.10.4160.10, 1 hit
    InterProiView protein in InterPro
    IPR012681 NCS1
    IPR001248 Pur-cyt_permease
    IPR038271 Pur-cyt_permease_sf
    PfamiView protein in Pfam
    PF02133 Transp_cyt_pur, 1 hit
    TIGRFAMsiTIGR00800 ncs1, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q08485-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSFSSIVSKF LRYLEIPAKN RTAVNFLRNP DLQPIKSANQ TWGFWSNLAY
    60 70 80 90 100
    WGAVSFTAGT WMSGSAALSV GLSYPETIVS FLLGNVLTII FTMANSYPGY
    110 120 130 140 150
    DWKIGFTLAQ RFVFGIYGSA FGIIIRILMS IVNYGSNAWL GGLSINMILD
    160 170 180 190 200
    SWSHHYLHLP NTLSPSVAMT TKQLVGFIIF HVLTALCYFM KPYHMNYLLI
    210 220 230 240 250
    WSCVATCFAM LGIVIYLTKN AHGVGELFTS TKSTVTGSKR AWAWVYMISY
    260 270 280 290 300
    WFGSISPGST NQSDYSRFGS SNLAIWTGSV CALLIPATLV PIFGVISAST
    310 320 330 340 350
    CDKLYGKQFW MPMDIFDYWL TNNYSAGARA GAFFCGLCFT MSQMSSTISN
    360 370 380 390 400
    CGFATGMDMA GLLPKYVDIK RGALFCACIS WACLPWNFYN SSSTFLTVMS
    410 420 430 440 450
    SFGVVMTPII AVMICDNFLI RKRQYSITNA FILKGEYYFT KGVNWRAIVA
    460 470 480 490 500
    WVCGMAPGLP GIAWEVNNNY FHDSGIVKFF YGDSFFSFLI SFFVYWGLCV
    510 520 530 540 550
    FFPFKITVRH DDKDYYGAFT DEEARKKGMI PYSEISEEEI RAYTLGECYT
    560 570 580 590
    TGHEYKPESS DNESPELIKT SSENTNVFEI VHQKDDEKHS FSTTQQVV
    Length:598
    Mass (Da):67,193
    Last modified:November 1, 1996 - v1
    Checksum:i7EB67439C7D5EB6B
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z70678 Genomic DNA Translation: CAA94556.1
    Z74979 Genomic DNA Translation: CAA99264.1
    BK006948 Genomic DNA Translation: DAA10850.1
    PIRiS66954
    RefSeqiNP_014714.1, NM_001183490.1

    Genome annotation databases

    EnsemblFungiiYOR071C; YOR071C; YOR071C
    GeneIDi854237
    KEGGisce:YOR071C

    Similar proteinsi

    Entry informationi

    Entry nameiNRT1_YEAST
    AccessioniPrimary (citable) accession number: Q08485
    Secondary accession number(s): D6W2D4, O00029
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: March 28, 2018
    This is version 128 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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