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Protein

Nicotinamide riboside transporter 1

Gene

NRT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity pH-dependent nicotinamide riboside transporter which also transports thiamine with low affinity. Involved in 5-fluorocytosine sensitivity.6 Publications

Kineticsi

  1. KM=21 µM for nicotinamide riboside1 Publication

    pH dependencei

    Optimum pH is 3.5-6.5.1 Publication

    GO - Molecular functioni

    • 5-amino-1-ribofuranosylimidazole-4-carboxamide transporter activity Source: SGD
    • nicotinamide riboside transmembrane transporter activity Source: SGD
    • nucleobase transmembrane transporter activity Source: GO_Central

    GO - Biological processi

    • 5-amino-1-ribofuranosylimidazole-4-carboxamide transmembrane transport Source: SGD
    • nicotinamide riboside transport Source: SGD
    • nucleobase transport Source: GO_Central
    • thiamine transport Source: SGD
    Complete GO annotation...

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33610-MONOMER.

    Protein family/group databases

    TCDBi2.A.39.4.2. the nucleobase:cation symporter-1 (ncs1) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nicotinamide riboside transporter 1
    Alternative name(s):
    Thiamine transport protein 71
    Gene namesi
    Name:NRT1
    Synonyms:THI71
    Ordered Locus Names:YOR071C
    ORF Names:YOR29-22
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XV

    Organism-specific databases

    EuPathDBiFungiDB:YOR071C.
    SGDiS000005597. NRT1.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei48 – 68HelicalSequence analysisAdd BLAST21
    Transmembranei71 – 91HelicalSequence analysisAdd BLAST21
    Transmembranei112 – 132HelicalSequence analysisAdd BLAST21
    Transmembranei174 – 194HelicalSequence analysisAdd BLAST21
    Transmembranei197 – 217HelicalSequence analysisAdd BLAST21
    Transmembranei241 – 261HelicalSequence analysisAdd BLAST21
    Transmembranei273 – 293HelicalSequence analysisAdd BLAST21
    Transmembranei372 – 392HelicalSequence analysisAdd BLAST21
    Transmembranei395 – 415HelicalSequence analysisAdd BLAST21
    Transmembranei447 – 467HelicalSequence analysisAdd BLAST21
    Transmembranei484 – 504HelicalSequence analysisAdd BLAST21

    GO - Cellular componenti

    • integral component of membrane Source: UniProtKB-KW
    • plasma membrane Source: SGD
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001979271 – 598Nicotinamide riboside transporter 1Add BLAST598

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei560PhosphoserineCombined sources1
    Modified residuei572PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ08485.
    PRIDEiQ08485.

    PTM databases

    iPTMnetiQ08485.

    Expressioni

    Inductioni

    Expression is controlled by the transcription repressors SUM1, HST1 and RFM1.1 Publication

    Interactioni

    Protein-protein interaction databases

    BioGridi34470. 22 interactors.
    DIPiDIP-7934N.
    IntActiQ08485. 1 interactor.
    MINTiMINT-1359499.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08485.
    SMRiQ08485.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00530000065049.
    InParanoidiQ08485.
    OMAiISASTCD.
    OrthoDBiEOG092C1JZY.

    Family and domain databases

    InterProiIPR012681. NCS1.
    IPR001248. Pur-cyt_permease.
    [Graphical view]
    PfamiPF02133. Transp_cyt_pur. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00800. ncs1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q08485-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSFSSIVSKF LRYLEIPAKN RTAVNFLRNP DLQPIKSANQ TWGFWSNLAY
    60 70 80 90 100
    WGAVSFTAGT WMSGSAALSV GLSYPETIVS FLLGNVLTII FTMANSYPGY
    110 120 130 140 150
    DWKIGFTLAQ RFVFGIYGSA FGIIIRILMS IVNYGSNAWL GGLSINMILD
    160 170 180 190 200
    SWSHHYLHLP NTLSPSVAMT TKQLVGFIIF HVLTALCYFM KPYHMNYLLI
    210 220 230 240 250
    WSCVATCFAM LGIVIYLTKN AHGVGELFTS TKSTVTGSKR AWAWVYMISY
    260 270 280 290 300
    WFGSISPGST NQSDYSRFGS SNLAIWTGSV CALLIPATLV PIFGVISAST
    310 320 330 340 350
    CDKLYGKQFW MPMDIFDYWL TNNYSAGARA GAFFCGLCFT MSQMSSTISN
    360 370 380 390 400
    CGFATGMDMA GLLPKYVDIK RGALFCACIS WACLPWNFYN SSSTFLTVMS
    410 420 430 440 450
    SFGVVMTPII AVMICDNFLI RKRQYSITNA FILKGEYYFT KGVNWRAIVA
    460 470 480 490 500
    WVCGMAPGLP GIAWEVNNNY FHDSGIVKFF YGDSFFSFLI SFFVYWGLCV
    510 520 530 540 550
    FFPFKITVRH DDKDYYGAFT DEEARKKGMI PYSEISEEEI RAYTLGECYT
    560 570 580 590
    TGHEYKPESS DNESPELIKT SSENTNVFEI VHQKDDEKHS FSTTQQVV
    Length:598
    Mass (Da):67,193
    Last modified:November 1, 1996 - v1
    Checksum:i7EB67439C7D5EB6B
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z70678 Genomic DNA. Translation: CAA94556.1.
    Z74979 Genomic DNA. Translation: CAA99264.1.
    BK006948 Genomic DNA. Translation: DAA10850.1.
    PIRiS66954.
    RefSeqiNP_014714.1. NM_001183490.1.

    Genome annotation databases

    EnsemblFungiiYOR071C; YOR071C; YOR071C.
    GeneIDi854237.
    KEGGisce:YOR071C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z70678 Genomic DNA. Translation: CAA94556.1.
    Z74979 Genomic DNA. Translation: CAA99264.1.
    BK006948 Genomic DNA. Translation: DAA10850.1.
    PIRiS66954.
    RefSeqiNP_014714.1. NM_001183490.1.

    3D structure databases

    ProteinModelPortaliQ08485.
    SMRiQ08485.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi34470. 22 interactors.
    DIPiDIP-7934N.
    IntActiQ08485. 1 interactor.
    MINTiMINT-1359499.

    Protein family/group databases

    TCDBi2.A.39.4.2. the nucleobase:cation symporter-1 (ncs1) family.

    PTM databases

    iPTMnetiQ08485.

    Proteomic databases

    MaxQBiQ08485.
    PRIDEiQ08485.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYOR071C; YOR071C; YOR071C.
    GeneIDi854237.
    KEGGisce:YOR071C.

    Organism-specific databases

    EuPathDBiFungiDB:YOR071C.
    SGDiS000005597. NRT1.

    Phylogenomic databases

    GeneTreeiENSGT00530000065049.
    InParanoidiQ08485.
    OMAiISASTCD.
    OrthoDBiEOG092C1JZY.

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33610-MONOMER.

    Miscellaneous databases

    PROiQ08485.

    Family and domain databases

    InterProiIPR012681. NCS1.
    IPR001248. Pur-cyt_permease.
    [Graphical view]
    PfamiPF02133. Transp_cyt_pur. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00800. ncs1. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiNRT1_YEAST
    AccessioniPrimary (citable) accession number: Q08485
    Secondary accession number(s): D6W2D4, O00029
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: November 30, 2016
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.