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Protein

GTPase-activating protein GYP1

Gene

GYP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates specifically the GTPase activity of YPT1. Functions on the Golgi as a negative regulator of YPT1.1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: SGD
  • Rab GTPase binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

BioCyciYEAST:G3O-33609-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase-activating protein GYP1
Alternative name(s):
GAP for YPT1
Gene namesi
Name:GYP1
Ordered Locus Names:YOR070C
ORF Names:YOR29-21
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR070C.
SGDiS000005596. GYP1.

Subcellular locationi

GO - Cellular componenti

  • Golgi apparatus Source: SGD
  • Golgi stack Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 637637GTPase-activating protein GYP1PRO_0000208010Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei69 – 691PhosphoserineCombined sources
Modified residuei250 – 2501PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08484.

PTM databases

iPTMnetiQ08484.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi34469. 317 interactions.
DIPiDIP-1258N.
IntActiQ08484. 6 interactions.
MINTiMINT-409462.

Structurei

Secondary structure

1
637
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi251 – 26212Combined sources
Helixi270 – 2778Combined sources
Helixi283 – 2853Combined sources
Helixi286 – 2938Combined sources
Helixi301 – 3033Combined sources
Helixi304 – 32118Combined sources
Beta strandi323 – 3264Combined sources
Helixi330 – 34011Combined sources
Helixi341 – 3433Combined sources
Helixi349 – 3524Combined sources
Helixi354 – 37017Combined sources
Turni372 – 3743Combined sources
Helixi380 – 39213Combined sources
Helixi393 – 3953Combined sources
Helixi398 – 4036Combined sources
Turni404 – 4063Combined sources
Helixi409 – 4113Combined sources
Helixi415 – 43319Combined sources
Helixi434 – 4396Combined sources
Helixi445 – 46117Combined sources
Helixi463 – 4719Combined sources
Helixi477 – 4859Combined sources
Turni486 – 4883Combined sources
Helixi489 – 4913Combined sources
Helixi494 – 50815Combined sources
Helixi571 – 58515Combined sources
Helixi587 – 5904Combined sources
Helixi595 – 6028Combined sources
Helixi612 – 62918Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FKMX-ray1.90A249-637[»]
2G77X-ray2.26A244-637[»]
ProteinModelPortaliQ08484.
SMRiQ08484. Positions 249-509, 569-630.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ08484.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini280 – 508229Rab-GAP TBCPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi117 – 12913His-richAdd
BLAST

Sequence similaritiesi

Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00840000129806.
HOGENOMiHOG000162526.
InParanoidiQ08484.
OMAiHQIEIDI.
OrthoDBiEOG7XSTQT.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 3 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08484-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVRSAAKEM HERDHNSDSS SLVTSLMKSW RISSASSSKK PSLYKMNTTE
60 70 80 90 100
STSLPSGYAS SADRDRRTSD GNFEAMAKQQ ASTRRTSNSY SPLRYVNPTL
110 120 130 140 150
STASNESPRP ALLLRQHHQR HHHHQQPRHS SSGSVGNNCS NSTEPNKKGD
160 170 180 190 200
RYFKDLDEDW SAVIDDYNMP IPILTNGGFG TPVAPTRTLS RKSTSSSINS
210 220 230 240 250
ISNMGTSAVR NSSSSFTYPQ LPQLQKEKTN DSKKTQLEIE NERDVQELNS
260 270 280 290 300
IIQRISKFDN ILKDKTIINQ QDLRQISWNG IPKIHRPVVW KLLIGYLPVN
310 320 330 340 350
TKRQEGFLQR KRKEYRDSLK HTFSDQHSRD IPTWHQIEID IPRTNPHIPL
360 370 380 390 400
YQFKSVQNSL QRILYLWAIR HPASGYVQGI NDLVTPFFET FLTEYLPPSQ
410 420 430 440 450
IDDVEIKDPS TYMVDEQITD LEADTFWCLT KLLEQITDNY IHGQPGILRQ
460 470 480 490 500
VKNLSQLVKR IDADLYNHFQ NEHVEFIQFA FRWMNCLLMR EFQMGTVIRM
510 520 530 540 550
WDTYLSETSQ EVTSSYSMSS NDIKPPVTPT EPRVASFVTP TKDFQSPTTA
560 570 580 590 600
LSNMTPNNAV EDSGKMRQSS LNEFHVFVCA AFLIKWSDQL MEMDFQETIT
610 620 630
FLQNPPTKDW TETDIEMLLS EAFIWQSLYK DATSHWL
Length:637
Mass (Da):73,289
Last modified:November 1, 1996 - v1
Checksum:i08C1A26B6ED37E13
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70678 Genomic DNA. Translation: CAA94555.1.
Z74978 Genomic DNA. Translation: CAA99263.1.
BK006948 Genomic DNA. Translation: DAA10849.1.
PIRiS66953.
RefSeqiNP_014713.1. NM_001183489.1.

Genome annotation databases

EnsemblFungiiYOR070C; YOR070C; YOR070C.
GeneIDi854236.
KEGGisce:YOR070C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70678 Genomic DNA. Translation: CAA94555.1.
Z74978 Genomic DNA. Translation: CAA99263.1.
BK006948 Genomic DNA. Translation: DAA10849.1.
PIRiS66953.
RefSeqiNP_014713.1. NM_001183489.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FKMX-ray1.90A249-637[»]
2G77X-ray2.26A244-637[»]
ProteinModelPortaliQ08484.
SMRiQ08484. Positions 249-509, 569-630.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34469. 317 interactions.
DIPiDIP-1258N.
IntActiQ08484. 6 interactions.
MINTiMINT-409462.

PTM databases

iPTMnetiQ08484.

Proteomic databases

MaxQBiQ08484.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR070C; YOR070C; YOR070C.
GeneIDi854236.
KEGGisce:YOR070C.

Organism-specific databases

EuPathDBiFungiDB:YOR070C.
SGDiS000005596. GYP1.

Phylogenomic databases

GeneTreeiENSGT00840000129806.
HOGENOMiHOG000162526.
InParanoidiQ08484.
OMAiHQIEIDI.
OrthoDBiEOG7XSTQT.

Enzyme and pathway databases

BioCyciYEAST:G3O-33609-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ08484.
PROiQ08484.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 3 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames."
    Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M.
    Yeast 13:379-390(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Yeast rab GTPase-activating protein Gyp1p localizes to the Golgi apparatus and is a negative regulator of Ypt1p."
    Du L.-L., Novick P.
    Mol. Biol. Cell 12:1215-1226(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-250, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-69 AND SER-250, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Crystal structure of the GAP domain of Gyp1p: first insights into interaction with Ypt/Rab proteins."
    Rak A., Fedorov R., Alexandrov K., Albert S., Goody R.S., Gallwitz D., Scheidig A.J.
    EMBO J. 19:5105-5113(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF RAB-GAP TBC DOMAIN.

Entry informationi

Entry nameiGYP1_YEAST
AccessioniPrimary (citable) accession number: Q08484
Secondary accession number(s): D6W2D3, O00028
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 206 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.