Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Platelet endothelial cell adhesion molecule

Gene

Pecam1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Modulates BDKRB2 activation (By similarity). Induces susceptibility to atherosclerosis.By similarity1 Publication

GO - Biological processi

  • angiogenesis Source: MGI
  • diapedesis Source: MGI
  • endothelial cell-matrix adhesion Source: MGI
  • endothelial cell migration Source: MGI
  • endothelial cell morphogenesis Source: MGI
  • phagocytosis Source: MGI
  • positive regulation of tyrosine phosphorylation of Stat5 protein Source: MGI
  • regulation of cell migration Source: MGI
  • Rho protein signal transduction Source: MGI
  • wound healing Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet endothelial cell adhesion molecule
Short name:
PECAM-1
Alternative name(s):
CD_antigen: CD31
Gene namesi
Name:Pecam1
Synonyms:Pecam, Pecam-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:97537. Pecam1.

Subcellular locationi

  • Cell membrane By similarity; Single-pass type I membrane protein By similarity
  • Membrane raft By similarity
  • Cell junction By similarity

  • Note: Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini18 – 590ExtracellularSequence analysisAdd BLAST573
Transmembranei591 – 609HelicalSequence analysisAdd BLAST19
Topological domaini610 – 727CytoplasmicSequence analysisAdd BLAST118

GO - Cellular componenti

  • cell junction Source: UniProtKB-SubCell
  • cell periphery Source: MGI
  • external side of plasma membrane Source: BHF-UCL
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: MGI
  • membrane raft Source: MGI
  • plasma membrane Source: MGI
  • smooth muscle contractile fiber Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice show reduced atherosclerotic lesions. There is down-regulation of ICAM-1 in endothelial cells at the lesion periphery, and reduced disruption of Cx43 junctional staining at arterial branch points and in the descending aorta.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi679Y → F: Reduces tyrosine phosphorylation by FER by about 60%. 1 Publication1
Mutagenesisi702Y → F: Reduces tyrosine phosphorylation by FER by about 60%. 1 Publication1
Mutagenesisi717Y → F: No significant effect on phosphorylation by FER. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 171 PublicationAdd BLAST17
ChainiPRO_000001489618 – 727Platelet endothelial cell adhesion moleculeAdd BLAST710

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi47 ↔ 99PROSITE-ProRule annotation
Glycosylationi74N-linked (GlcNAc...)1 Publication1
Glycosylationi141N-linked (GlcNAc...)1 Publication1
Disulfide bondi142 ↔ 195PROSITE-ProRule annotation
Disulfide bondi245 ↔ 293PROSITE-ProRule annotation
Glycosylationi309N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi336 ↔ 375PROSITE-ProRule annotation
Glycosylationi345N-linked (GlcNAc...)Sequence analysis1
Glycosylationi360N-linked (GlcNAc...)1 Publication1
Disulfide bondi420 ↔ 465PROSITE-ProRule annotation
Glycosylationi424N-linked (GlcNAc...)1 Publication1
Disulfide bondi512 ↔ 561PROSITE-ProRule annotation
Glycosylationi540N-linked (GlcNAc...)1 Publication1
Lipidationi611S-palmitoyl cysteineBy similarity1
Modified residuei679Phosphotyrosine; by FER1 Publication1
Modified residuei702Phosphotyrosine; by FERCombined sources1 Publication1

Post-translational modificationi

Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation (By similarity). Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation.By similarity1 Publication
Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiQ08481.
PaxDbiQ08481.
PeptideAtlasiQ08481.
PRIDEiQ08481.

PTM databases

iPTMnetiQ08481.
PhosphoSitePlusiQ08481.
SwissPalmiQ08481.

Expressioni

Tissue specificityi

Isoform 1 and isoform 3 are expressed in lung and platelets.1 Publication

Gene expression databases

BgeeiENSMUSG00000020717.
CleanExiMM_PECAM1.
ExpressionAtlasiQ08481. baseline and differential.
GenevisibleiQ08481. MM.

Interactioni

Subunit structurei

Interacts with PTPN11; Tyr-702 is critical for PTPN11 recruitment. Forms a complex with BDKRB2 and GNAQ. Interacts with BDKRB2 and GNAQ (By similarity).By similarity

Protein-protein interaction databases

IntActiQ08481. 5 interactors.
MINTiMINT-4106994.
STRINGi10090.ENSMUSP00000079664.

Structurei

3D structure databases

ProteinModelPortaliQ08481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 126Ig-like C2-type 1Add BLAST87
Domaini135 – 213Ig-like C2-type 2Add BLAST79
Domaini225 – 309Ig-like C2-type 3Add BLAST85
Domaini315 – 391Ig-like C2-type 4Add BLAST77
Domaini413 – 472Ig-like C2-type 5Add BLAST60
Domaini488 – 578Ig-like C2-type 6Add BLAST91

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni710 – 727May play a role in cytoprotective signalingBy similarityAdd BLAST18

Domaini

The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.By similarity

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II37. Eukaryota.
ENOG4111F9A. LUCA.
GeneTreeiENSGT00440000034155.
HOGENOMiHOG000049132.
HOVERGENiHBG059434.
InParanoidiQ08481.
KOiK06471.
TreeFamiTF338229.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF13895. Ig_2. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 4 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 4 hits.
PROSITEiPS50835. IG_LIKE. 3 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q08481-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLALGLTLV LYASLQAEEN SFTINSIHME SLPSWEVMNG QQLTLECLVD
60 70 80 90 100
ISTTSKSRSQ HRVLFYKDDA MVYNVTSREH TESYVIPQAR VFHSGKYKCT
110 120 130 140 150
VMLNNKEKTT IEYEVKVHGV SKPKVTLDKK EVTEGGVVTV NCSLQEEKPP
160 170 180 190 200
IFFKIEKLEV GTKFVKRRID KTSNENFVLM EFPIEAQDHV LVFRCQAGIL
210 220 230 240 250
SGFKLQESEP IRSEYVTVQE SFSTPKFEIK PPGMIIEGDQ LHIRCIVQVT
260 270 280 290 300
HLVQEFTEII IQKDKAIVAT SKQSSEAVYS VMAMVEYSGH YTCKVESNRI
310 320 330 340 350
SKASSIMVNI TELFPKPKLE FSSSRLDQGE LLDLSCSVSG TPVANFTIQK
360 370 380 390 400
EETVLSQYQN FSKIAEESDS GEYSCTAGIG KVVKRSGLVP IQVCEMLSKP
410 420 430 440 450
SIFHDAKSEI IKGHAIGISC QSENGTAPIT YHLMKAKSDF QTLEVTSNDP
460 470 480 490 500
ATFTDKPTRD MEYQCRADNC HSHPAVFSEI LRVRVIAPVD EVVISILSSN
510 520 530 540 550
EVQSGSEMVL RCSVKEGTSP ITFQFYKEKE DRPFHQAVVN DTQAFWHNKQ
560 570 580 590 600
ASKKQEGQYY CTASNRASSM RTSPRSSTLA VRVFLAPWKK GLIAVVVIGV
610 620 630 640 650
VIATLIVAAK CYFLRKAKAK QKPVEMSRPA APLLNSNSEK ISEPSVEANS
660 670 680 690 700
HYGYDDVSGN DAVKPINQNK DPQNMDVEYT EVEVSSLEPH QALGTRATET
710 720
VYSEIRKVDP NLMENRYSRT EGSLNGT
Length:727
Mass (Da):81,263
Last modified:October 1, 1994 - v1
Checksum:i34C04752D199BAA5
GO
Isoform 2 (identifier: Q08481-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     692-727: ALGTRATETVYSEIRKVDPNLMENRYSRTEGSLNGT → ENGRLP

Show »
Length:697
Mass (Da):77,917
Checksum:iEF8538025C1AB55F
GO
Isoform 3 (identifier: Q08481-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     711-727: NLMENRYSRTEGSLNGT → KNGRLP

Show »
Length:716
Mass (Da):80,004
Checksum:iE4D281B5523017B6
GO
Isoform 4 (identifier: Q08481-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     119-219: Missing.

Show »
Length:626
Mass (Da):69,823
Checksum:i0CF4D64C49CE468B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18E → T AA sequence (PubMed:1415479).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038723119 – 219Missing in isoform 4. 1 PublicationAdd BLAST101
Alternative sequenceiVSP_038724692 – 727ALGTR…SLNGT → ENGRLP in isoform 2. 1 PublicationAdd BLAST36
Alternative sequenceiVSP_038725711 – 727NLMEN…SLNGT → KNGRLP in isoform 3. CuratedAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06039 mRNA. Translation: AAA16230.1.
AK169431 mRNA. Translation: BAE41172.1.
AL603664 Genomic DNA. Translation: CAM18558.1.
AL603664 Genomic DNA. Translation: CAM18559.1.
AL603664 Genomic DNA. Translation: CAM18560.1.
CH466558 Genomic DNA. Translation: EDL34303.1.
CH466558 Genomic DNA. Translation: EDL34304.1.
CH466558 Genomic DNA. Translation: EDL34305.1.
BC008519 mRNA. Translation: AAH08519.1.
BC085502 mRNA. Translation: AAH85502.1.
CCDSiCCDS25558.1. [Q08481-2]
CCDS25559.1. [Q08481-3]
CCDS79068.1. [Q08481-4]
CCDS79069.1. [Q08481-1]
RefSeqiNP_001027550.1. NM_001032378.2. [Q08481-2]
NP_001292086.1. NM_001305157.1. [Q08481-1]
NP_001292087.1. NM_001305158.1. [Q08481-4]
NP_032842.2. NM_008816.3. [Q08481-3]
XP_011247094.1. XM_011248792.1. [Q08481-1]
UniGeneiMm.343951.

Genome annotation databases

EnsembliENSMUST00000080853; ENSMUSP00000079664; ENSMUSG00000020717. [Q08481-3]
ENSMUST00000103069; ENSMUSP00000099358; ENSMUSG00000020717. [Q08481-2]
ENSMUST00000106796; ENSMUSP00000102408; ENSMUSG00000020717. [Q08481-1]
ENSMUST00000183610; ENSMUSP00000138959; ENSMUSG00000020717. [Q08481-4]
GeneIDi18613.
KEGGimmu:18613.
UCSCiuc007lze.2. mouse. [Q08481-1]
uc007lzf.2. mouse. [Q08481-3]
uc007lzg.2. mouse. [Q08481-2]
uc007lzh.2. mouse. [Q08481-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

PECAM-1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06039 mRNA. Translation: AAA16230.1.
AK169431 mRNA. Translation: BAE41172.1.
AL603664 Genomic DNA. Translation: CAM18558.1.
AL603664 Genomic DNA. Translation: CAM18559.1.
AL603664 Genomic DNA. Translation: CAM18560.1.
CH466558 Genomic DNA. Translation: EDL34303.1.
CH466558 Genomic DNA. Translation: EDL34304.1.
CH466558 Genomic DNA. Translation: EDL34305.1.
BC008519 mRNA. Translation: AAH08519.1.
BC085502 mRNA. Translation: AAH85502.1.
CCDSiCCDS25558.1. [Q08481-2]
CCDS25559.1. [Q08481-3]
CCDS79068.1. [Q08481-4]
CCDS79069.1. [Q08481-1]
RefSeqiNP_001027550.1. NM_001032378.2. [Q08481-2]
NP_001292086.1. NM_001305157.1. [Q08481-1]
NP_001292087.1. NM_001305158.1. [Q08481-4]
NP_032842.2. NM_008816.3. [Q08481-3]
XP_011247094.1. XM_011248792.1. [Q08481-1]
UniGeneiMm.343951.

3D structure databases

ProteinModelPortaliQ08481.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ08481. 5 interactors.
MINTiMINT-4106994.
STRINGi10090.ENSMUSP00000079664.

PTM databases

iPTMnetiQ08481.
PhosphoSitePlusiQ08481.
SwissPalmiQ08481.

Proteomic databases

MaxQBiQ08481.
PaxDbiQ08481.
PeptideAtlasiQ08481.
PRIDEiQ08481.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000080853; ENSMUSP00000079664; ENSMUSG00000020717. [Q08481-3]
ENSMUST00000103069; ENSMUSP00000099358; ENSMUSG00000020717. [Q08481-2]
ENSMUST00000106796; ENSMUSP00000102408; ENSMUSG00000020717. [Q08481-1]
ENSMUST00000183610; ENSMUSP00000138959; ENSMUSG00000020717. [Q08481-4]
GeneIDi18613.
KEGGimmu:18613.
UCSCiuc007lze.2. mouse. [Q08481-1]
uc007lzf.2. mouse. [Q08481-3]
uc007lzg.2. mouse. [Q08481-2]
uc007lzh.2. mouse. [Q08481-4]

Organism-specific databases

CTDi5175.
MGIiMGI:97537. Pecam1.

Phylogenomic databases

eggNOGiENOG410II37. Eukaryota.
ENOG4111F9A. LUCA.
GeneTreeiENSGT00440000034155.
HOGENOMiHOG000049132.
HOVERGENiHBG059434.
InParanoidiQ08481.
KOiK06471.
TreeFamiTF338229.

Miscellaneous databases

ChiTaRSiPecam1. mouse.
PROiQ08481.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020717.
CleanExiMM_PECAM1.
ExpressionAtlasiQ08481. baseline and differential.
GenevisibleiQ08481. MM.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF13895. Ig_2. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 4 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 4 hits.
PROSITEiPS50835. IG_LIKE. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPECA1_MOUSE
AccessioniPrimary (citable) accession number: Q08481
Secondary accession number(s): B1ARB1
, B1ARB2, Q3TES6, Q922E0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.