Leukotriene-B(4) omega-hydroxylase 2

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Reviewed, UniProtKB/Swiss-Prot Q08477 (CP4F3_HUMAN)

Last modified July 7, 2009. Version 93. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Leukotriene-B(4) omega-hydroxylase 2
    EC=1.14.13.30
Alternative name(s):
    Cytochrome P450 4F3
    CYPIVF3
    Leukotriene-B(4) 20-monooxygenase 2
    Cytochrome P450-LTB-omega

Gene names

Name:	CYP4F3
Synonyms:	LTB4H

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. This enzyme requires molecular oxygen and NADPH for the omega-hydroxylation of LTB4, a potent chemoattractant for polymorphonuclear leukocytes.
Catalytic activity	(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O₂ = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP⁺ + H₂O.
Cofactor	Heme group By similarity.
Enzyme regulation	Inhibited by carbon monoxide (CO).
Pathway	Lipid metabolism; leukotriene B4 degradation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Microsome membrane; Single-pass membrane protein By similarity.
Tissue specificity	Expressed in the polymorphonuclear leukocytes as well as leukocytes.
Sequence similarities	Belongs to the cytochrome P450 family.
Sequence caution	The sequence AAC08589.1 differs from that shown. Reason: Frameshift at positions 67 and 114.

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Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leukotriene metabolic process Ref.1 Traceable author statement. Source: ProtInc oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro leukotriene-B4 20-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 520

520

Leukotriene-B(4) omega-hydroxylase 2

PRO_0000051851

Regions

Transmembrane

11 – 31

Potential

Sites

Metal binding

468

Iron (heme axial ligand) By similarity

Binding site

328

Heme (covalent; via 1 link) By similarity

Natural variations

Natural variant

H → Q: dbSNP rs34923393.

VAR_048457

Natural variant

106

Y → C: dbSNP rs35888783.

VAR_048458

Natural variant

269

A → D: dbSNP rs1805040. Ref.1 Ref.2 Ref.4

VAR_001258

Natural variant

270

V → I Ref.4

VAR_020664

Natural variant

271

I → T Ref.4

VAR_020665

Experimental info

Sequence conflict

184 – 186

EGS → KGY in AAC08589. Ref.3

Sequence conflict

488

R → A Ref.1

Sequence conflict

488

R → A Ref.2

Sequence conflict

512

L → I in AAC08589. Ref.3

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Sequences

Sequence

Length

Mass (Da)

Tools

Q08477-1 [UniParc].

Last modified December 21, 2004. Version 2.
Checksum: 2519D875280CF9DC
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FASTA

520

59,847

        10         20         30         40         50         60 
MPQLSLSSLG LWPMAASPWL LLLLVGASWL LARILAWTYT FYDNCCRLRC FPQPPKRNWF 

        70         80         90        100        110        120 
LGHLGLIHSS EEGLLYTQSL ACTFGDMCCW WVGPWHAIVR IFHPTYIKPV LFAPAAIVPK 

       130        140        150        160        170        180 
DKVFYSFLKP WLGDGLLLSA GEKWSRHRRM LTPAFHFNIL KPYMKIFNES VNIMHAKWQL 

       190        200        210        220        230        240 
LASEGSARLD MFEHISLMTL DSLQKCVFSF DSHCQEKPSE YIAAILELSA LVTKRHQQIL 

       250        260        270        280        290        300 
LYIDFLYYLT PDGQRFRRAC RLVHDFTDAV IQERRRTLPS QGVDDFLQAK AKSKTLDFID 

       310        320        330        340        350        360 
VLLLSKDEDG KKLSDEDIRA EADTFMFEGH DTTASGLSWV LYHLAKHPEY QERCRQEVQE 

       370        380        390        400        410        420 
LLKDREPKEI EWDDLAQLPF LTMCIKESLR LHPPVPAVSR CCTQDIVLPD GRVIPKGIIC 

       430        440        450        460        470        480 
LISVFGTHHN PAVWPDPEVY DPFRFDPKNI KERSPLAFIP FSAGPRNCIG QAFAMAEMKV 

       490        500        510        520 
VLGLTLLRFR VLPDHTEPRR KPELVLRAEG GLWLRVEPLS

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References

[1]	"A novel form of cytochrome P-450 family 4 in human polymorphonuclear leukocytes. cDNA cloning and expression of leukotriene B4 omega-hydroxylase." Kikuta Y., Kusunose E., Endo K., Yamamoto S., Sogawa K., Fujii-Kuriyama Y., Kusunose M. J. Biol. Chem. 268:9376-9380(1993) [PubMed: 8486631] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASP-269. Tissue: Leukocyte.
[2]	"Human leukotriene B4 omega-hydroxylase (CYP4F3) gene: molecular cloning and chromosomal localization." Kikuta Y., Kato M., Yamashita Y., Miyauchi Y., Tanaka K., Kamada N., Kusunose M. DNA Cell Biol. 17:221-230(1998) [PubMed: 9539102] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT ASP-269.
[3]	"A novel form of cytochrome P-450 family 4 in human fetal liver." Peng X., Morgan K., Morgan T.R. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal liver.
[4]	SeattleSNPs variation discovery resource Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ASP-269; ILE-270 AND THR-271.
+	Additional computationally mapped references.

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Web resources

SeattleSNPs

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Cross-references

Sequence databases
	D12620 mRNA. Translation: BAA02144.1. D12621 mRNA. Translation: BAA02145.1. AB002454 mRNA. Translation: BAA25990.1. AB002461 Genomic DNA. Translation: BAA25991.1. AF054821 mRNA. Translation: AAC08589.1. Frameshift. AY792513 Genomic DNA. Translation: AAV40834.1.
IPI	IPI00002370.
PIR	A46661.
RefSeq	NP_000887.2.
UniGene	Hs.106242
3D structure databases
HSSP	HSSP built from PDB template 1JME based on UniProtKB P14779.
ModBase	Search...
Proteomic databases
PRIDE	Q08477.
Genome annotation databases
Ensembl	ENSG00000186529. Homo sapiens. [Contig view]
GeneID	4051.
KEGG	hsa:4051.
NMPDR	fig\|9606.3.peg.15898.
UCSC	uc002nbj.1. human.
Organism-specific databases
GeneCards	GC19P015612.
H-InvDB	HIX0058774.
HGNC	HGNC:2646. CYP4F3.
MIM	601270. gene.
PharmGKB	PA234.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q08477.
HOVERGEN	Q08477.
OMA	Q08477. QHHGERR.
Enzyme and pathway databases
BRENDA	1.14.13.30. 247.
Reactome	REACT_13433. Biological oxidations.
Gene expression databases
ArrayExpress	Q08477.
Bgee	Q08477.
CleanEx	HS_CYP4F3.
GermOnline	ENSG00000186529. Homo sapiens.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	15870.
SOURCE	Search...

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Entry information

Entry name

CP4F3_HUMAN

Accession

Primary (citable) accession number: Q08477
Secondary accession number(s): O60634, Q5U740

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	December 21, 2004
Last modified:	July 7, 2009
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Natural variations

Experimental info

Sequences

References

Web resources

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents