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Q08471 (MSA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G1-specific transcription factors activator MSA1
Alternative name(s):
MBF and SBF-associated protein 1
Gene names
Name:MSA1
Ordered Locus Names:YOR066W
ORF Names:YOR29-17
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length629 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Activator of G1-specific transcription factors, MBF and SBF. Promotes both the timing of G1-specific gene transcription and cell cycle initiation. Associates with SBF- and MBF-regulated target promoters and this binding is maximal during the G1 phase, prior to maximum budding. Affects cell cycle initiation by advancing the timing of transcription of G1-specific genes. Overexpression advances the timing of SBF-dependent transcription and budding. Depletion delays both indicators of cell cycle initiation. Ref.6 Ref.7

Subunit structure

Interacts with transcription complexes SCB-binding factor (SBF) and MCB-binding factor (MBF) at their target promoters. Interacts with MBP1 and SWI6. Ref.7

Developmental stage

Expressed periodically during the cell cycle with peak mRNA levels occurring at the late M/early G1 phase, prior to budding, and levels decrease rapidly as cells enter into the S phase. In early G1 peak occurs (at protein level). Ref.7

Post-translational modification

Phosphorylated by CDC28. Ref.5

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 629629G1-specific transcription factors activator MSA1
PRO_0000237651

Regions

Compositional bias317 – 33317Asn-rich
Compositional bias446 – 47530Gln-rich

Amino acid modifications

Modified residue2681Phosphoserine Ref.9

Sequences

Sequence LengthMass (Da)Tools
Q08471 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 302204DCC8ECFD0D

FASTA62968,691
        10         20         30         40         50         60 
MDKSMIKKRG RPPITKDYPN PLQSPMAHSS MQVQKQGPHS FAKPLMKVGQ SSPSPNKRRL 

        70         80         90        100        110        120 
SIDHHHNLAA TTRKGRYRGV LLSTPTKKSS TNGSTPISTP SSNDSYNNTV FSETRKTFLQ 

       130        140        150        160        170        180 
SSPPIMTSSP AFQKKNDYMF PSQEQFKLSL TITESGKAVI AGSLPFSPSS KSSHLMNNNN 

       190        200        210        220        230        240 
KKIMQNEKIH KGSKKNAPKF EKRRILSLLK QMKNEKYCDT DTLPEAPPAK PSRSDIIDTE 

       250        260        270        280        290        300 
LPTIIETSAS PIGSARNNNI LLSQPPQSPP SSAQLKPPST PKSSLQFRMG FTPNVALNSV 

       310        320        330        340        350        360 
SLSDTISKST NAVGASNNNN QNGNSISNIA DANTLLTLTN SPGVFLSPRN KMLPKSTTAS 

       370        380        390        400        410        420 
NEQQQEFVFK FSSGDPLLLT DDADGNWPEM LFNVSNTPRR QKCFNTPPSW INFGSPGLFS 

       430        440        450        460        470        480 
PPRSSNVMVN GTTVATASDS GNVHRQLQAQ LEAQVQVQSQ SNSPTQRQQQ QRQFQIPPPH 

       490        500        510        520        530        540 
INMNSSPPQI NIASPPHQSM SRVSSIYFNK EKTTTGVANM LGNTKSENLQ PPANLFTAAH 

       550        560        570        580        590        600 
GPSTPRNQEF QLPTLIECTP LIQQTMNGSL GTKYIPGTSI SNSATPNLHG FPVGTGKAPS 

       610        620 
SFDDSLKQNP YSNKQDDART ALKRLIDDQ 

« Hide

References

« Hide 'large scale' references
[1]"The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames."
Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M.
Yeast 13:379-390(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Targets of the cyclin-dependent kinase Cdk1."
Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O.
Nature 425:859-864(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY CDC28.
[6]"Transcriptional activators in yeast."
Titz B., Thomas S., Rajagopala S.V., Chiba T., Ito T., Uetz P.
Nucleic Acids Res. 34:955-967(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"The SBF- and MBF-associated protein Msa1 is required for proper timing of G1-specific transcription in Saccharomyces cerevisiae."
Ashe M., de Bruin R.A.M., Kalashnikova T., McDonald W.H., Yates J.R. III, Wittenberg C.
J. Biol. Chem. 283:6040-6049(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH MBP1; SWI6; SBF AND MBF COMPLEXES, DEVELOPMENTAL STAGE.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-268, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z70678 Genomic DNA. Translation: CAA94551.1.
Z74974 Genomic DNA. Translation: CAA99259.1.
AY692621 Genomic DNA. Translation: AAT92640.1.
BK006948 Genomic DNA. Translation: DAA10845.1.
PIRS66949.
RefSeqNP_014709.1. NM_001183485.1.

3D structure databases

ProteinModelPortalQ08471.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34465. 38 interactions.
DIPDIP-2714N.
IntActQ08471. 4 interactions.
MINTMINT-553172.
STRING4932.YOR066W.

Proteomic databases

PaxDbQ08471.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR066W; YOR066W; YOR066W.
GeneID854232.
KEGGsce:YOR066W.

Organism-specific databases

CYGDYOR066w.
SGDS000005592. MSA1.

Phylogenomic databases

eggNOGNOG45219.
HOGENOMHOG000113659.
OMAPPSWINF.
OrthoDBEOG7R2BXD.

Enzyme and pathway databases

BioCycYEAST:G3O-33606-MONOMER.

Gene expression databases

GenevestigatorQ08471.

Family and domain databases

ProtoNetSearch...

Other

NextBio976118.

Entry information

Entry nameMSA1_YEAST
AccessionPrimary (citable) accession number: Q08471
Secondary accession number(s): D6W2C9, O00026
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD