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Protein

G1-specific transcription factors activator MSA1

Gene

MSA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Activator of G1-specific transcription factors, MBF and SBF. Promotes both the timing of G1-specific gene transcription and cell cycle initiation. Associates with SBF- and MBF-regulated target promoters and this binding is maximal during the G1 phase, prior to maximum budding. Affects cell cycle initiation by advancing the timing of transcription of G1-specific genes. Overexpression advances the timing of SBF-dependent transcription and budding. Depletion delays both indicators of cell cycle initiation.2 Publications

GO - Biological processi

  1. G1/S transition of mitotic cell cycle Source: SGD
  2. regulation of cell size Source: SGD
  3. regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-33606-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
G1-specific transcription factors activator MSA1
Alternative name(s):
MBF and SBF-associated protein 1
Gene namesi
Name:MSA1
Ordered Locus Names:YOR066W
ORF Names:YOR29-17
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR066w.
SGDiS000005592. MSA1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 629629G1-specific transcription factors activator MSA1PRO_0000237651Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei268 – 2681Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated by CDC28.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08471.
PaxDbiQ08471.

Expressioni

Developmental stagei

Expressed periodically during the cell cycle with peak mRNA levels occurring at the late M/early G1 phase, prior to budding, and levels decrease rapidly as cells enter into the S phase. In early G1 peak occurs (at protein level).1 Publication

Gene expression databases

GenevestigatoriQ08471.

Interactioni

Subunit structurei

Interacts with transcription complexes SCB-binding factor (SBF) and MCB-binding factor (MBF) at their target promoters. Interacts with MBP1 and SWI6.1 Publication

Protein-protein interaction databases

BioGridi34465. 43 interactions.
DIPiDIP-2714N.
IntActiQ08471. 4 interactions.
MINTiMINT-553172.
STRINGi4932.YOR066W.

Structurei

3D structure databases

ProteinModelPortaliQ08471.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi317 – 33317Asn-richAdd
BLAST
Compositional biasi446 – 47530Gln-richAdd
BLAST

Phylogenomic databases

eggNOGiNOG45219.
HOGENOMiHOG000113659.
InParanoidiQ08471.
OMAiPPSWINF.
OrthoDBiEOG7R2BXD.

Sequencei

Sequence statusi: Complete.

Q08471-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDKSMIKKRG RPPITKDYPN PLQSPMAHSS MQVQKQGPHS FAKPLMKVGQ
60 70 80 90 100
SSPSPNKRRL SIDHHHNLAA TTRKGRYRGV LLSTPTKKSS TNGSTPISTP
110 120 130 140 150
SSNDSYNNTV FSETRKTFLQ SSPPIMTSSP AFQKKNDYMF PSQEQFKLSL
160 170 180 190 200
TITESGKAVI AGSLPFSPSS KSSHLMNNNN KKIMQNEKIH KGSKKNAPKF
210 220 230 240 250
EKRRILSLLK QMKNEKYCDT DTLPEAPPAK PSRSDIIDTE LPTIIETSAS
260 270 280 290 300
PIGSARNNNI LLSQPPQSPP SSAQLKPPST PKSSLQFRMG FTPNVALNSV
310 320 330 340 350
SLSDTISKST NAVGASNNNN QNGNSISNIA DANTLLTLTN SPGVFLSPRN
360 370 380 390 400
KMLPKSTTAS NEQQQEFVFK FSSGDPLLLT DDADGNWPEM LFNVSNTPRR
410 420 430 440 450
QKCFNTPPSW INFGSPGLFS PPRSSNVMVN GTTVATASDS GNVHRQLQAQ
460 470 480 490 500
LEAQVQVQSQ SNSPTQRQQQ QRQFQIPPPH INMNSSPPQI NIASPPHQSM
510 520 530 540 550
SRVSSIYFNK EKTTTGVANM LGNTKSENLQ PPANLFTAAH GPSTPRNQEF
560 570 580 590 600
QLPTLIECTP LIQQTMNGSL GTKYIPGTSI SNSATPNLHG FPVGTGKAPS
610 620
SFDDSLKQNP YSNKQDDART ALKRLIDDQ
Length:629
Mass (Da):68,691
Last modified:November 1, 1996 - v1
Checksum:i302204DCC8ECFD0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70678 Genomic DNA. Translation: CAA94551.1.
Z74974 Genomic DNA. Translation: CAA99259.1.
AY692621 Genomic DNA. Translation: AAT92640.1.
BK006948 Genomic DNA. Translation: DAA10845.1.
PIRiS66949.
RefSeqiNP_014709.1. NM_001183485.1.

Genome annotation databases

EnsemblFungiiYOR066W; YOR066W; YOR066W.
GeneIDi854232.
KEGGisce:YOR066W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70678 Genomic DNA. Translation: CAA94551.1.
Z74974 Genomic DNA. Translation: CAA99259.1.
AY692621 Genomic DNA. Translation: AAT92640.1.
BK006948 Genomic DNA. Translation: DAA10845.1.
PIRiS66949.
RefSeqiNP_014709.1. NM_001183485.1.

3D structure databases

ProteinModelPortaliQ08471.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34465. 43 interactions.
DIPiDIP-2714N.
IntActiQ08471. 4 interactions.
MINTiMINT-553172.
STRINGi4932.YOR066W.

Proteomic databases

MaxQBiQ08471.
PaxDbiQ08471.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR066W; YOR066W; YOR066W.
GeneIDi854232.
KEGGisce:YOR066W.

Organism-specific databases

CYGDiYOR066w.
SGDiS000005592. MSA1.

Phylogenomic databases

eggNOGiNOG45219.
HOGENOMiHOG000113659.
InParanoidiQ08471.
OMAiPPSWINF.
OrthoDBiEOG7R2BXD.

Enzyme and pathway databases

BioCyciYEAST:G3O-33606-MONOMER.

Miscellaneous databases

NextBioi976118.

Gene expression databases

GenevestigatoriQ08471.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames."
    Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M.
    Yeast 13:379-390(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: PHOSPHORYLATION BY CDC28.
  6. Cited for: FUNCTION.
  7. "The SBF- and MBF-associated protein Msa1 is required for proper timing of G1-specific transcription in Saccharomyces cerevisiae."
    Ashe M., de Bruin R.A.M., Kalashnikova T., McDonald W.H., Yates J.R. III, Wittenberg C.
    J. Biol. Chem. 283:6040-6049(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH MBP1; SWI6; SBF AND MBF COMPLEXES, DEVELOPMENTAL STAGE.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-268, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMSA1_YEAST
AccessioniPrimary (citable) accession number: Q08471
Secondary accession number(s): D6W2C9, O00026
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: November 1, 1996
Last modified: February 4, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.