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Q08471

- MSA1_YEAST

UniProt

Q08471 - MSA1_YEAST

Protein

G1-specific transcription factors activator MSA1

Gene

MSA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Activator of G1-specific transcription factors, MBF and SBF. Promotes both the timing of G1-specific gene transcription and cell cycle initiation. Associates with SBF- and MBF-regulated target promoters and this binding is maximal during the G1 phase, prior to maximum budding. Affects cell cycle initiation by advancing the timing of transcription of G1-specific genes. Overexpression advances the timing of SBF-dependent transcription and budding. Depletion delays both indicators of cell cycle initiation.2 Publications

    GO - Biological processi

    1. G1/S transition of mitotic cell cycle Source: SGD
    2. regulation of cell size Source: SGD
    3. regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Cell cycle, Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33606-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G1-specific transcription factors activator MSA1
    Alternative name(s):
    MBF and SBF-associated protein 1
    Gene namesi
    Name:MSA1
    Ordered Locus Names:YOR066W
    ORF Names:YOR29-17
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR066w.
    SGDiS000005592. MSA1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nucleus Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 629629G1-specific transcription factors activator MSA1PRO_0000237651Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei268 – 2681Phosphoserine2 Publications

    Post-translational modificationi

    Phosphorylated by CDC28.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ08471.
    PaxDbiQ08471.

    Expressioni

    Developmental stagei

    Expressed periodically during the cell cycle with peak mRNA levels occurring at the late M/early G1 phase, prior to budding, and levels decrease rapidly as cells enter into the S phase. In early G1 peak occurs (at protein level).1 Publication

    Gene expression databases

    GenevestigatoriQ08471.

    Interactioni

    Subunit structurei

    Interacts with transcription complexes SCB-binding factor (SBF) and MCB-binding factor (MBF) at their target promoters. Interacts with MBP1 and SWI6.1 Publication

    Protein-protein interaction databases

    BioGridi34465. 41 interactions.
    DIPiDIP-2714N.
    IntActiQ08471. 4 interactions.
    MINTiMINT-553172.
    STRINGi4932.YOR066W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08471.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi317 – 33317Asn-richAdd
    BLAST
    Compositional biasi446 – 47530Gln-richAdd
    BLAST

    Phylogenomic databases

    eggNOGiNOG45219.
    HOGENOMiHOG000113659.
    OMAiPPSWINF.
    OrthoDBiEOG7R2BXD.

    Sequencei

    Sequence statusi: Complete.

    Q08471-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDKSMIKKRG RPPITKDYPN PLQSPMAHSS MQVQKQGPHS FAKPLMKVGQ    50
    SSPSPNKRRL SIDHHHNLAA TTRKGRYRGV LLSTPTKKSS TNGSTPISTP 100
    SSNDSYNNTV FSETRKTFLQ SSPPIMTSSP AFQKKNDYMF PSQEQFKLSL 150
    TITESGKAVI AGSLPFSPSS KSSHLMNNNN KKIMQNEKIH KGSKKNAPKF 200
    EKRRILSLLK QMKNEKYCDT DTLPEAPPAK PSRSDIIDTE LPTIIETSAS 250
    PIGSARNNNI LLSQPPQSPP SSAQLKPPST PKSSLQFRMG FTPNVALNSV 300
    SLSDTISKST NAVGASNNNN QNGNSISNIA DANTLLTLTN SPGVFLSPRN 350
    KMLPKSTTAS NEQQQEFVFK FSSGDPLLLT DDADGNWPEM LFNVSNTPRR 400
    QKCFNTPPSW INFGSPGLFS PPRSSNVMVN GTTVATASDS GNVHRQLQAQ 450
    LEAQVQVQSQ SNSPTQRQQQ QRQFQIPPPH INMNSSPPQI NIASPPHQSM 500
    SRVSSIYFNK EKTTTGVANM LGNTKSENLQ PPANLFTAAH GPSTPRNQEF 550
    QLPTLIECTP LIQQTMNGSL GTKYIPGTSI SNSATPNLHG FPVGTGKAPS 600
    SFDDSLKQNP YSNKQDDART ALKRLIDDQ 629
    Length:629
    Mass (Da):68,691
    Last modified:November 1, 1996 - v1
    Checksum:i302204DCC8ECFD0D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z70678 Genomic DNA. Translation: CAA94551.1.
    Z74974 Genomic DNA. Translation: CAA99259.1.
    AY692621 Genomic DNA. Translation: AAT92640.1.
    BK006948 Genomic DNA. Translation: DAA10845.1.
    PIRiS66949.
    RefSeqiNP_014709.1. NM_001183485.1.

    Genome annotation databases

    EnsemblFungiiYOR066W; YOR066W; YOR066W.
    GeneIDi854232.
    KEGGisce:YOR066W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z70678 Genomic DNA. Translation: CAA94551.1 .
    Z74974 Genomic DNA. Translation: CAA99259.1 .
    AY692621 Genomic DNA. Translation: AAT92640.1 .
    BK006948 Genomic DNA. Translation: DAA10845.1 .
    PIRi S66949.
    RefSeqi NP_014709.1. NM_001183485.1.

    3D structure databases

    ProteinModelPortali Q08471.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34465. 41 interactions.
    DIPi DIP-2714N.
    IntActi Q08471. 4 interactions.
    MINTi MINT-553172.
    STRINGi 4932.YOR066W.

    Proteomic databases

    MaxQBi Q08471.
    PaxDbi Q08471.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR066W ; YOR066W ; YOR066W .
    GeneIDi 854232.
    KEGGi sce:YOR066W.

    Organism-specific databases

    CYGDi YOR066w.
    SGDi S000005592. MSA1.

    Phylogenomic databases

    eggNOGi NOG45219.
    HOGENOMi HOG000113659.
    OMAi PPSWINF.
    OrthoDBi EOG7R2BXD.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33606-MONOMER.

    Miscellaneous databases

    NextBioi 976118.

    Gene expression databases

    Genevestigatori Q08471.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames."
      Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M.
      Yeast 13:379-390(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: PHOSPHORYLATION BY CDC28.
    6. Cited for: FUNCTION.
    7. "The SBF- and MBF-associated protein Msa1 is required for proper timing of G1-specific transcription in Saccharomyces cerevisiae."
      Ashe M., de Bruin R.A.M., Kalashnikova T., McDonald W.H., Yates J.R. III, Wittenberg C.
      J. Biol. Chem. 283:6040-6049(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH MBP1; SWI6; SBF AND MBF COMPLEXES, DEVELOPMENTAL STAGE.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-268, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMSA1_YEAST
    AccessioniPrimary (citable) accession number: Q08471
    Secondary accession number(s): D6W2C9, O00026
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3