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Q08469 (S6A15_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium-dependent neutral amino acid transporter B(0)AT2
Alternative name(s):
Solute carrier family 6 member 15
Transporter v7-3
sodium- and chloride-dependent neurotransmitter transporter NTT73
Gene names
Name:Slc6a15
Synonyms:Ntt73
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length729 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Functions as a sodium-dependent neutral amino acid transporter. Exhibits preference for methionine and for the branched-chain amino acids, particularly leucine, valine and isoleucine. Mediates the saturable, pH-sensitive and electrogenic cotransport of proline and sodium ions with a stoichiometry of 1:1. May have a role as transporter for neurotransmitter precursors into neurons. In contrast to other members of the neurotransmitter transporter family, does not appear to be chloride-dependent By similarity.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Tissue specificity

Widely distributed in the central nervous system, including the olfactory bulb, the hypothalamus, the cerebral cortex, the hippocampus, and the cerebellum. In addition, intense expression is found in the motor nuclei including the oculomotor nucleus, abducens nucleus, trigeminal motor nucleus, facial nucleus, hypoglossal nucleus and ventral horn of spinal cord. Intense hybridization signals are also observed in the nuclei containing monoaminergic neurons, such as locus coeruleus, the substantia nigra pars compacta, the ventral tegmental area, the dorsal raphe nucleus and the median raphe nucleus. Ref.2

Sequence similarities

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A15 subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 729729Sodium-dependent neutral amino acid transporter B(0)AT2
PRO_0000214800

Regions

Topological domain1 – 6969Cytoplasmic Potential
Transmembrane70 – 9021Helical; Name=1; Potential
Transmembrane98 – 11720Helical; Name=2; Potential
Transmembrane142 – 16221Helical; Name=3; Potential
Topological domain163 – 22563Extracellular Potential
Transmembrane226 – 24419Helical; Name=4; Potential
Transmembrane253 – 27018Helical; Name=5; Potential
Transmembrane306 – 32318Helical; Name=6; Potential
Transmembrane335 – 35622Helical; Name=7; Potential
Topological domain357 – 45296Extracellular Potential
Transmembrane453 – 47220Helical; Name=8; Potential
Transmembrane496 – 51419Helical; Name=9; Potential
Transmembrane530 – 55021Helical; Name=10; Potential
Transmembrane571 – 59222Helical; Name=11; Potential
Transmembrane620 – 64223Helical; Name=12; Potential
Topological domain643 – 72987Cytoplasmic Potential

Amino acid modifications

Modified residue551Phosphoserine By similarity
Modified residue6871Phosphoserine By similarity
Glycosylation1871N-linked (GlcNAc...) Potential
Glycosylation3831N-linked (GlcNAc...) Potential
Glycosylation3941N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict1 – 33MPK → IP in AAB25532. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q08469 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: ECDDC6403741B53E

FASTA72981,596
        10         20         30         40         50         60 
MPKNSKVVKR DLDDDVIESV KDLLSNEDSV EDVSKKSELI VDVQEEKDTD AEDGSEVDDE 

        70         80         90        100        110        120 
RPAWNSKLQY ILAQVGFSVG LGNVWRFPYL CQKNGGGAYL LPYLILLLVI GIPLFFLELS 

       130        140        150        160        170        180 
VGQRIRRGSI GVWNYISPKL GGIGFASCVV CYFVALYYNV IIGWTLFYFS QSFQQPLPWD 

       190        200        210        220        230        240 
QCPLVKNASH TYIEPECEKS SATTYYWYRE ALAISSSISE SGGLNWKMTG CLLAAWVMVC 

       250        260        270        280        290        300 
LAMIKGIQSS GKIMYFSSLF PYVVLICFLI RSLLLNGSID GIRHMFTPKL EMMLEPKVWR 

       310        320        330        340        350        360 
EAATQVFFAL GLGFGGVIAF SSYNKRDNNC HFDAVLVSFI NFFTSVLATL VVFAVLGFKA 

       370        380        390        400        410        420 
NIVNEKCISQ NSEMILKLLK TGNVSWDVIP RHINLSAVTA EDYHVVYDII QKVKEEEFAV 

       430        440        450        460        470        480 
LHLKACQIED ELNKAVQGTG LAFIAFTEAM THFPASPFWS VMFFLMLINL GLGSMFGTIE 

       490        500        510        520        530        540 
GIITPVVDTF KVRKEILTVI CCLLAFCIGL MFVQRSGNYF VTMFDDYSAT LPLLIVVILE 

       550        560        570        580        590        600 
NIAVSFVYGI DKFLEDLTDM LGFAPSKYYY YMWKYISPLM LVTLLIASIV NMGLSPPGYN 

       610        620        630        640        650        660 
AWIKEKASEE FLSYPMWGMV VCFSLMVLAI LPVPVVFVIR RCNLIDDSSG NLASVTYKRG 

       670        680        690        700        710        720 
RVLKEPVNLD GDDASLIHGK IPSEMSSPNF GKNIYRKQSG SPTLDTAPNG RYGIGYLMAD 


MPDMPESDL

« Hide

References

[1]"Neurotransmitter transporter family cDNAs in a rat midbrain library: 'orphan transporters' suggest sizable structural variations."
Uhl G.R., Kitayama S., Gregor P., Nanthakumar E., Persico A.M., Shimada S.
Brain Res. Mol. Brain Res. 16:353-359(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Widespread brain distribution of mRNA encoding the orphan neurotransmitter transporter v7-3."
Inoue K., Sato K., Tohyama M., Shimada S., Uhl G.R.
Brain Res. Mol. Brain Res. 37:217-223(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L22022 mRNA. Translation: AAA41729.1.
S56968 mRNA. Translation: AAB25532.1.
IPIIPI00200616.
PIRI65413.
RefSeqNP_758824.1. NM_172321.1.
UniGeneRn.9716.

3D structure databases

ProteinModelPortalQ08469.
ModBaseSearch...

Proteomic databases

PRIDEQ08469.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000029208; ENSRNOP00000032996; ENSRNOG00000027468.
GeneID282712.
KEGGrno:282712.
UCSCRGD:628664. rat.

Organism-specific databases

CTD55117.
RGD628664. Slc6a15.

Phylogenomic databases

eggNOGCOG0733.
GeneTreeENSGT00660000095315.
HOGENOMHOG000116406.
HOVERGENHBG071421.
InParanoidQ08469.
KOK05048.
OMANSCQIED.
OrthoDBEOG4N04DJ.

Gene expression databases

GenevestigatorQ08469.
GermOnlineENSRNOG00000027468. Rattus norvegicus.

Family and domain databases

InterProIPR000175. Na/ntran_symport.
IPR002438. Na/ntran_symport_orphan.
[Graphical view]
PANTHERPTHR11616. PTHR11616. 1 hit.
PfamPF00209. SNF. 1 hit.
[Graphical view]
PRINTSPR00176. NANEUSMPORT.
PR01206. ORPHTRNSPORT.
PROSITEPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio624845.

Entry information

Entry nameS6A15_RAT
AccessionPrimary (citable) accession number: Q08469
Secondary accession number(s): Q63838
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 3, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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