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Protein

Casein kinase II subunit alpha-1

Gene

CKA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha chain contains the catalytic site. May act as an ectokinase that phosphorylates several extracellular proteins.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei139 – 1391ATPPROSITE-ProRule annotation
Active sitei227 – 2271Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi116 – 1249ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G67380-MONOMER.
ARA:GQT-143-MONOMER.
BRENDAi2.7.11.1. 399.
ReactomeiR-ATH-2514853. Condensation of Prometaphase Chromosomes.

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase II subunit alpha-1 (EC:2.7.11.1)
Short name:
CK II
Alternative name(s):
Casein kinase alpha 1
Gene namesi
Name:CKA1
Ordered Locus Names:At5g67380
ORF Names:K8K14.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G67380.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Sequence analysisAdd
BLAST
Chaini36 – 409374Casein kinase II subunit alpha-1PRO_0000417491Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi72 – 721N-linked (GlcNAc...)Sequence analysis
Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ08467.
PRIDEiQ08467.

Expressioni

Tissue specificityi

Seems to be present in all plant organs. But seem to be less expressed than CKA2.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.Curated

Binary interactionsi

WithEntry#Exp.IntActNotes
CCA1P929734EBI-1644972,EBI-1644880

Protein-protein interaction databases

BioGridi22115. 7 interactions.
IntActiQ08467. 4 interactions.
STRINGi3702.AT5G67380.1.

Structurei

3D structure databases

ProteinModelPortaliQ08467.
SMRiQ08467. Positions 78-402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini110 – 395286Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0668. Eukaryota.
ENOG410XNPP. LUCA.
HOGENOMiHOG000233021.
InParanoidiQ08467.
KOiK03097.
OMAiHYERYTA.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q08467-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIDTLFFLFF LFFDSPLRRL LLLCAVLALR APTAHSPILR SSIVTPTARA
60 70 80 90 100
VSEVSGCTTI DPDFLVEISD SNQTRAMSKA RVYTEVNVIR PKDYWDYESL
110 120 130 140 150
IVQWGEQDDY EVVRKVGRGK YSEVFEGINV NSKEKCIIKI LKPVKKKKIR
160 170 180 190 200
REIKILQNLC GGPNIVKLLD VVRDQHSKTP SLIFEYVNST DFKVLYPTLT
210 220 230 240 250
DYDIRYYIYE LLKALDFCHS QGIMHRDVKP HNVMIDHELR KLRLIDWGLA
260 270 280 290 300
EFYHPGKEYN VRVASRYFKG PELLVDLQDY DYSLDMWSLG CMFAGMIFRK
310 320 330 340 350
EPFFYGHDNQ DQLVKIAKVL GTDELNAYLN KYQLELDPQL EALVGRHSRK
360 370 380 390 400
PWSKFINADN QHLVSPEAID FLDKLLRYDH QDRLTAKEAM AHAYFAQVRA

AETSRMRSQ
Note: No experimental confirmation available.
Length:409
Mass (Da):47,640
Last modified:May 16, 2012 - v3
Checksum:i35F553B9CDA53D33
GO
Isoform 2 (identifier: Q08467-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     115-147: Missing.

Note: No experimental confirmation available.
Show »
Length:376
Mass (Da):43,896
Checksum:iC3062E99125504CB
GO
Isoform 3 (identifier: Q08467-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.

Note: No experimental confirmation available.
Show »
Length:333
Mass (Da):39,258
Checksum:i8F765E82C6CD617E
GO
Isoform 4 (identifier: Q08467-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-223: Missing.

Note: No experimental confirmation available.
Show »
Length:186
Mass (Da):21,933
Checksum:i6483BDFD4F227809
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti223 – 2231I → L in BAA01090 (PubMed:7678767).Curated
Sequence conflicti264 – 2641A → V in BAA01090 (PubMed:7678767).Curated
Sequence conflicti296 – 2972MI → LL in BAA01090 (PubMed:7678767).Curated
Sequence conflicti319 – 3202VL → GV in BAA01090 (PubMed:7678767).Curated
Sequence conflicti349 – 3491R → G in BAH20017 (PubMed:19423640).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 223223Missing in isoform 4. 1 PublicationVSP_043757Add
BLAST
Alternative sequencei1 – 7676Missing in isoform 3. 1 PublicationVSP_043758Add
BLAST
Alternative sequencei115 – 14733Missing in isoform 2. 1 PublicationVSP_043759Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10246 mRNA. Translation: BAA01090.1.
AB007645 Genomic DNA. Translation: BAB09023.1.
CP002688 Genomic DNA. Translation: AED98334.1.
CP002688 Genomic DNA. Translation: AED98335.1.
AF386966 mRNA. Translation: AAK62411.1.
BT002141 mRNA. Translation: AAN72152.1.
AK317345 mRNA. Translation: BAH20017.1.
AK317458 mRNA. Translation: BAH20125.1.
PIRiS31098.
RefSeqiNP_001032165.1. NM_001037088.2. [Q08467-2]
NP_201539.2. NM_126138.3. [Q08467-1]
UniGeneiAt.24323.

Genome annotation databases

EnsemblPlantsiAT5G67380.1; AT5G67380.1; AT5G67380. [Q08467-1]
GeneIDi836873.
KEGGiath:AT5G67380.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10246 mRNA. Translation: BAA01090.1.
AB007645 Genomic DNA. Translation: BAB09023.1.
CP002688 Genomic DNA. Translation: AED98334.1.
CP002688 Genomic DNA. Translation: AED98335.1.
AF386966 mRNA. Translation: AAK62411.1.
BT002141 mRNA. Translation: AAN72152.1.
AK317345 mRNA. Translation: BAH20017.1.
AK317458 mRNA. Translation: BAH20125.1.
PIRiS31098.
RefSeqiNP_001032165.1. NM_001037088.2. [Q08467-2]
NP_201539.2. NM_126138.3. [Q08467-1]
UniGeneiAt.24323.

3D structure databases

ProteinModelPortaliQ08467.
SMRiQ08467. Positions 78-402.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22115. 7 interactions.
IntActiQ08467. 4 interactions.
STRINGi3702.AT5G67380.1.

Proteomic databases

PaxDbiQ08467.
PRIDEiQ08467.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G67380.1; AT5G67380.1; AT5G67380. [Q08467-1]
GeneIDi836873.
KEGGiath:AT5G67380.

Organism-specific databases

TAIRiAT5G67380.

Phylogenomic databases

eggNOGiKOG0668. Eukaryota.
ENOG410XNPP. LUCA.
HOGENOMiHOG000233021.
InParanoidiQ08467.
KOiK03097.
OMAiHYERYTA.

Enzyme and pathway databases

BioCyciARA:AT5G67380-MONOMER.
ARA:GQT-143-MONOMER.
BRENDAi2.7.11.1. 399.
ReactomeiR-ATH-2514853. Condensation of Prometaphase Chromosomes.

Miscellaneous databases

PROiQ08467.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of two cDNAs encoding casein kinase II catalytic subunits in Arabidopsis thaliana."
    Mizoguchi T., Yamaguchi-Shinozaki K., Hayashida N., Kamada H., Shinozaki K.
    Plant Mol. Biol. 21:279-289(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
    Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
    Strain: cv. Columbia.
  5. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: cv. Columbia.

Entry informationi

Entry nameiCSK21_ARATH
AccessioniPrimary (citable) accession number: Q08467
Secondary accession number(s): B9DH00
, B9DHA8, F4K3Q1, Q94F17, Q9FN14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: May 16, 2012
Last modified: May 11, 2016
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.