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Protein

Casein kinase II subunit alpha-2

Gene

CKA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha chain contains the catalytic site. May act as an ectokinase that phosphorylates several extracellular proteins.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei133 – 1331ATPPROSITE-ProRule annotation
Active sitei221 – 2211Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi110 – 1189ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G50000-MONOMER.
BRENDAi2.7.11.1. 399.
ReactomeiR-ATH-2514853. Condensation of Prometaphase Chromosomes.
R-ATH-6804756. Regulation of TP53 Activity through Phosphorylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase II subunit alpha-2 (EC:2.7.11.1)
Short name:
CK II
Gene namesi
Name:CKA2
Ordered Locus Names:At3g50000
ORF Names:F3A4.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G50000.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence analysisAdd
BLAST
Chaini32 – 403372Casein kinase II subunit alpha-2PRO_0000417492Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi182 – 1821N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ08466.
PRIDEiQ08466.

Expressioni

Tissue specificityi

Seems to be present in all plant organs. But seem to be more expressed than CKA1.

Gene expression databases

GenevisibleiQ08466. AT.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.Curated

Protein-protein interaction databases

BioGridi9480. 4 interactions.
IntActiQ08466. 1 interaction.
STRINGi3702.AT3G50000.1.

Structurei

3D structure databases

ProteinModelPortaliQ08466.
SMRiQ08466. Positions 40-396.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini104 – 389286Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0668. Eukaryota.
ENOG410XNPP. LUCA.
HOGENOMiHOG000233021.
InParanoidiQ08466.
KOiK03097.
OMAiLYQYIDK.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q08466-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHLIFFFSYF LRRYLLLLCA ILILRAPLAH SLIPPLTCVN TGTVESDVTG
60 70 80 90 100
IRFDRCLDTD SLAKISLSTV MSKARVYTDV NVIRPKDYWD YESLNVQWGE
110 120 130 140 150
QDDYEVVRKV GRGKYSEVFE GINMNNNEKC IIKILKPVKK KKIRREIKIL
160 170 180 190 200
QNLCGGPNIV KLLDVVRDQH SKTPSLIFEY VNSTDFKVLY PTLTDYDIRY
210 220 230 240 250
YIYELLKALD FCHSQGIMHR DVKPHNVMID HELRKLRLID WGLAEFYHPG
260 270 280 290 300
KEYNVRVASR YFKGPELLVD LQDYDYSLDM WSLGCMFAGM IFRKEPFFYG
310 320 330 340 350
HDNQDQLVKI AKVLGTDELN AYLNKYQLEL DTQLEALVGR HSRKPWSKFI
360 370 380 390 400
NADNRHLVSP EAIDYLDKLL RYDHQDRLTA KEAMAHPYFA QVRAAESSRM

RTQ
Note: No experimental confirmation available.
Length:403
Mass (Da):47,233
Last modified:May 16, 2012 - v3
Checksum:i495EA87AF560FE83
GO
Isoform 2 (identifier: Q08466-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.

Note: No experimental confirmation available.
Show »
Length:333
Mass (Da):39,364
Checksum:iB98664E89A52A9EB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti142 – 1421K → E in BAA01091 (PubMed:7678767).Curated
Sequence conflicti232 – 2321E → G in BAA01091 (PubMed:7678767).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7070Missing in isoform 2. 1 PublicationVSP_043760Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10247 mRNA. Translation: BAA01091.1.
AL132978 Genomic DNA. Translation: CAB62108.1.
CP002686 Genomic DNA. Translation: AEE78615.1.
AF370308 mRNA. Translation: AAK44123.2.
BT000888 mRNA. Translation: AAN41288.1.
PIRiS31099.
T45853.
RefSeqiNP_190569.2. NM_114860.3. [Q08466-1]
UniGeneiAt.268.

Genome annotation databases

EnsemblPlantsiAT3G50000.1; AT3G50000.1; AT3G50000. [Q08466-1]
GeneIDi824162.
KEGGiath:AT3G50000.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10247 mRNA. Translation: BAA01091.1.
AL132978 Genomic DNA. Translation: CAB62108.1.
CP002686 Genomic DNA. Translation: AEE78615.1.
AF370308 mRNA. Translation: AAK44123.2.
BT000888 mRNA. Translation: AAN41288.1.
PIRiS31099.
T45853.
RefSeqiNP_190569.2. NM_114860.3. [Q08466-1]
UniGeneiAt.268.

3D structure databases

ProteinModelPortaliQ08466.
SMRiQ08466. Positions 40-396.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9480. 4 interactions.
IntActiQ08466. 1 interaction.
STRINGi3702.AT3G50000.1.

Proteomic databases

PaxDbiQ08466.
PRIDEiQ08466.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G50000.1; AT3G50000.1; AT3G50000. [Q08466-1]
GeneIDi824162.
KEGGiath:AT3G50000.

Organism-specific databases

TAIRiAT3G50000.

Phylogenomic databases

eggNOGiKOG0668. Eukaryota.
ENOG410XNPP. LUCA.
HOGENOMiHOG000233021.
InParanoidiQ08466.
KOiK03097.
OMAiLYQYIDK.

Enzyme and pathway databases

BioCyciARA:AT3G50000-MONOMER.
BRENDAi2.7.11.1. 399.
ReactomeiR-ATH-2514853. Condensation of Prometaphase Chromosomes.
R-ATH-6804756. Regulation of TP53 Activity through Phosphorylation.

Miscellaneous databases

PROiQ08466.

Gene expression databases

GenevisibleiQ08466. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of two cDNAs encoding casein kinase II catalytic subunits in Arabidopsis thaliana."
    Mizoguchi T., Yamaguchi-Shinozaki K., Hayashida N., Kamada H., Shinozaki K.
    Plant Mol. Biol. 21:279-289(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.

Entry informationi

Entry nameiCSK22_ARATH
AccessioniPrimary (citable) accession number: Q08466
Secondary accession number(s): Q8H120, Q8H765, Q9SN18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: May 16, 2012
Last modified: June 8, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.