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Protein

Frizzled-1

Gene

Fzd1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activation by Wnt8 induces expression of beta-catenin target genes.

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: GO_Central
  2. identical protein binding Source: IntAct
  3. protein heterodimerization activity Source: BHF-UCL
  4. protein homodimerization activity Source: BHF-UCL
  5. Wnt-activated receptor activity Source: GO_Central
  6. Wnt-protein binding Source: GO_Central

GO - Biological processi

  1. canonical Wnt signaling pathway Source: BHF-UCL
  2. cellular response to growth factor stimulus Source: RGD
  3. cellular response to peptide hormone stimulus Source: RGD
  4. cellular response to vitamin D Source: RGD
  5. lung alveolus development Source: RGD
  6. non-canonical Wnt signaling pathway Source: GO_Central
  7. positive regulation of sequence-specific DNA binding transcription factor activity Source: BHF-UCL
  8. regulation of osteoblast differentiation Source: RGD
  9. Wnt signaling pathway Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Frizzled-1
Short name:
Fz-1
Short name:
rFz1
Gene namesi
Name:Fzd1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61916. Fzd1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini69 – 316248ExtracellularSequence AnalysisAdd
BLAST
Transmembranei317 – 33721Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini338 – 34811CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei349 – 36921Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini370 – 39627ExtracellularSequence AnalysisAdd
BLAST
Transmembranei397 – 41721Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini418 – 43922CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei440 – 46021Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini461 – 48323ExtracellularSequence AnalysisAdd
BLAST
Transmembranei484 – 50421Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini505 – 53026CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei531 – 55121Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini552 – 59544ExtracellularSequence AnalysisAdd
BLAST
Transmembranei596 – 61621Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini617 – 64125CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: GO_Central
  2. neuron projection Source: RGD
  3. plasma membrane Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 6868Sequence AnalysisAdd
BLAST
Chaini69 – 641573Frizzled-1PRO_0000012975Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi111 ↔ 172PROSITE-ProRule annotation
Disulfide bondi119 ↔ 165PROSITE-ProRule annotation
Glycosylationi125 – 1251N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi156 ↔ 192PROSITE-ProRule annotation
Disulfide bondi182 ↔ 221PROSITE-ProRule annotation
Disulfide bondi186 ↔ 209PROSITE-ProRule annotation
Glycosylationi225 – 2251N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ08463.
PRIDEiQ08463.

PTM databases

PhosphoSiteiQ08463.

Expressioni

Tissue specificityi

Widely expressed. Most abundant in kidney, liver, uterus, ovary and heart. Lower levels seen in brain and intestine. Extremely low in calvaria, mammary glands and testis.

Developmental stagei

Expressed predominantly in neonatal tissues, at lower levels in adult.

Gene expression databases

GenevestigatoriQ08463.

Interactioni

Subunit structurei

Interacts with MYOC.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-8766455,EBI-8766455
Fzd2Q084646EBI-8766455,EBI-7402050

Protein-protein interaction databases

IntActiQ08463. 1 interaction.
STRINGi10116.ENSRNOP00000021979.

Structurei

3D structure databases

SMRiQ08463. Positions 109-225.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini106 – 224119FZPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi619 – 6246Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family membersBy similarity
Motifi639 – 6413PDZ-binding

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi85 – 906Poly-Pro

Domaini

Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.By similarity
The FZ domain is involved in binding with Wnt ligands.By similarity

Sequence similaritiesi

Contains 1 FZ (frizzled) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG257258.
HOGENOMiHOG000233236.
HOVERGENiHBG006977.
InParanoidiQ08463.
PhylomeDBiQ08463.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026548. FZD1.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF81. PTHR11309:SF81. 1 hit.
PfamiPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSiPR00489. FRIZZLED.
SMARTiSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08463-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEEAVPSES RAAGRPSLEL CAVALPGRRE EVGHQDTAGH RRPRAHSRCW
60 70 80 90 100
ARGLLLLLWL LEAPLLLGVR AQPAGQVSGP GQQRPPPPQP QQGGQQYNGE
110 120 130 140 150
RGISIPDHGY CQPISIPLCT DIAYNQTIMP NLLGHTNQED AGLEVHQFYP
160 170 180 190 200
LVKVQCSAEL KFFLCSMYAP VCTVLEQALP PCRSLCERAQ GCEALMNKFG
210 220 230 240 250
FQWPDTLKCE KFPVHGAGEL CVGQNTSDKG TPTPSLLPEF WTSNPQHGGG
260 270 280 290 300
GYRGGYPGGA GPVERGKFSC PRALRVPSYL NYHFLGEKDC GAPCEPTKVY
310 320 330 340 350
GLMYFGPEEL RFSRTWIGIW SVLCCASTLF TVLTYLVDMR RFSYPERPII
360 370 380 390 400
FLSGCYTAVA VAYIAGFLLE DRVVCNDKFA EDGARTVAQG TKKEGCTILF
410 420 430 440 450
MMLYFFSMAS SIWWVILSLT WFLAAGMKWG HEAIEANSQY FHLAAWAVPA
460 470 480 490 500
IKTITILALG QVDGDVLSGV CFVGLNNVDA LRGFVLAPLF VYLFIGTSFL
510 520 530 540 550
LAGFVSLFRI RTIMKHDGTK TEKLEKLMVR IGVFSVLYTV PATIVIACYF
560 570 580 590 600
YEQAFRDQWE RSWVAQSCKS YAIPCPHLQG GGGVPPHPPM SPDFTVFMIK
610 620 630 640
YLMTLIVGIT SGFWIWSGKT LNSWRKFYTR LTNSKQGETT V
Length:641
Mass (Da):71,027
Last modified:November 1, 1996 - v1
Checksum:iD82E2C113E81B8B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02529 mRNA. Translation: AAA41173.1.
PIRiA45054.
UniGeneiRn.6575.

Genome annotation databases

UCSCiRGD:61916. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02529 mRNA. Translation: AAA41173.1.
PIRiA45054.
UniGeneiRn.6575.

3D structure databases

SMRiQ08463. Positions 109-225.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ08463. 1 interaction.
STRINGi10116.ENSRNOP00000021979.

Chemistry

GuidetoPHARMACOLOGYi229.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ08463.

Proteomic databases

PaxDbiQ08463.
PRIDEiQ08463.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:61916. rat.

Organism-specific databases

RGDi61916. Fzd1.

Phylogenomic databases

eggNOGiNOG257258.
HOGENOMiHOG000233236.
HOVERGENiHBG006977.
InParanoidiQ08463.
PhylomeDBiQ08463.

Miscellaneous databases

PROiQ08463.

Gene expression databases

GenevestigatoriQ08463.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026548. FZD1.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF81. PTHR11309:SF81. 1 hit.
PfamiPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSiPR00489. FRIZZLED.
SMARTiSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Two homologs of the Drosophila polarity gene frizzled (fz) are widely expressed in mammalian tissues."
    Chan S.D.H., Karpf D.B., Fowlkes M.E., Hooks M., Bradley M.S., Vuong V., Bambino T., Liu M.Y.C., Arnaud C.D., Strewler G.J., Nissenson R.A.
    J. Biol. Chem. 267:25202-25207(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Osteosarcoma.
  2. "Protein kinase C is differentially stimulated by Wnt and Frizzled homologs in a G-protein-dependent manner."
    Sheldahl L.C., Park M., Malbon C.C., Moon R.T.
    Curr. Biol. 9:695-698(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: COUPLING TO BETA-CATENIN PATHWAY.

Entry informationi

Entry nameiFZD1_RAT
AccessioniPrimary (citable) accession number: Q08463
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: November 1, 1996
Last modified: April 1, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.