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Q08446

- SGT1_YEAST

UniProt

Q08446 - SGT1_YEAST

Protein

Protein SGT1

Gene

SGT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Required for both entry into S phase and kinetochore function. Also involved in cyclic AMP (cAMP) pathway, possibly by participating in the assembly or the conformational activation of specific multiprotein complexes.2 Publications

    GO - Molecular functioni

    1. chaperone binding Source: SGD
    2. protein binding Source: IntAct
    3. protein binding, bridging Source: SGD

    GO - Biological processi

    1. cAMP-mediated signaling Source: SGD
    2. cell cycle Source: UniProtKB-KW
    3. kinetochore assembly Source: SGD
    4. protein complex assembly Source: SGD
    5. protein ubiquitination Source: SGD
    6. regulation of cell cycle Source: SGD

    Keywords - Biological processi

    Cell cycle, Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33597-MONOMER.
    ReactomeiREACT_189238. The NLRP3 inflammasome.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein SGT1
    Alternative name(s):
    Suppressor of G2 allele of SKP1
    Gene namesi
    Name:SGT1
    Ordered Locus Names:YOR057W
    ORF Names:YOR29-08
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR057w.
    SGDiS000005583. SGT1.

    Subcellular locationi

    GO - Cellular componenti

    1. ubiquitin ligase complex Source: SGD

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi31 – 311L → P in sgt1-3; induces arrest of cell division in G2/M; when associated with L-99 and I-213.
    Mutagenesisi99 – 991F → L in sgt1-3; induces arrest of cell division in G2/M; when associated with P-31 I-213.
    Mutagenesisi213 – 2131N → I in sgt1-3; induces arrest of cell division in G2/M; when associated with P-31 and L-99.
    Mutagenesisi220 – 2201D → V in sgt1-5; induces arrest of cell division in G2/M; when associated with K-364.
    Mutagenesisi364 – 3641E → K in sgt1-5; induces arrest of cell division in G2/M; when associated with V-220.
    Mutagenesisi371 – 3711S → N in A364a; suppressor of the cdc35-1 allele.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 395395Protein SGT1PRO_0000185393Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei168 – 1681Phosphoserine3 Publications
    Modified residuei171 – 1711Phosphoserine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ08446.
    PaxDbiQ08446.
    PeptideAtlasiQ08446.
    PRIDEiQ08446.

    Expressioni

    Gene expression databases

    GenevestigatoriQ08446.

    Interactioni

    Subunit structurei

    Interacts with SKP1/CBF3D. Part of SCF E3 ubiquitin ligase complexes containing SKP1, CDC53, HRT1 and some F-box proteins. Interacts with CIR1/CDC35.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CTF13P352033EBI-17070,EBI-4085
    HSP82P028292EBI-17070,EBI-8659
    SKP1P522866EBI-17070,EBI-4090

    Protein-protein interaction databases

    BioGridi34455. 40 interactions.
    DIPiDIP-1628N.
    IntActiQ08446. 10 interactions.
    MINTiMINT-400978.
    STRINGi4932.YOR057W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08446.
    SMRiQ08446. Positions 11-77, 92-140, 184-277.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini182 – 27796CSPROSITE-ProRule annotationAdd
    BLAST
    Domaini312 – 39584SGSPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CS domain.PROSITE-ProRule annotation
    Contains 1 SGS domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5091.
    GeneTreeiENSGT00390000013700.
    HOGENOMiHOG000141743.
    KOiK12795.
    OMAiITIYAKN.
    OrthoDBiEOG7RBZMH.

    Family and domain databases

    Gene3Di1.25.40.10. 1 hit.
    2.60.40.790. 2 hits.
    InterProiIPR007052. CS_dom.
    IPR008978. HSP20-like_chaperone.
    IPR007699. SGS.
    IPR011990. TPR-like_helical.
    [Graphical view]
    PfamiPF04969. CS. 1 hit.
    PF05002. SGS. 1 hit.
    [Graphical view]
    SUPFAMiSSF49764. SSF49764. 2 hits.
    PROSITEiPS51203. CS. 1 hit.
    PS51048. SGS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q08446-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPVEKDLKTA YKALYDEKEP LKALHLYDEI LKGSPTNLTA LIFKAACLEK    50
    LYFGFSDWHS DATMENAKEL LDKALMTAEG RGDRSKIGLV NFRYFVHFFN 100
    IKDYELAQSY FKKAKNLGYV DDTLPLWEDR LETKLNKKNK KQKDSTNKHT 150
    IKPVESIENR GDNNSSHSPI SPLKIETAPQ ESPKFKIDWY QSSTSVTISL 200
    FTVNLPESKE QVNIYISPND RRTLSISYQV PKSGSEFQYN AKLSHEVDPK 250
    AVSLKIFPKK LEITLSKIDS TQWKKLEEDI LTESSRLSDE GKNSDSATRL 300
    LSAETASKER LSYPSSSKKK IDWSKLDIDE EADEEAGSAD SFFQKLYAGA 350
    DPDTKRAMMK SFIESNGTAL STDWEDVSKG TVKTSPPEGM EPKHW 395
    Length:395
    Mass (Da):44,860
    Last modified:November 1, 1996 - v1
    Checksum:i5DE65874BE155552
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131A → T in AAS56369. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U88830 Genomic DNA. Translation: AAB48841.1.
    Z70678 Genomic DNA. Translation: CAA94542.1.
    Z74965 Genomic DNA. Translation: CAA99250.1.
    AY558043 Genomic DNA. Translation: AAS56369.1.
    BK006948 Genomic DNA. Translation: DAA10836.1.
    PIRiS66940.
    RefSeqiNP_014700.1. NM_001183476.1.

    Genome annotation databases

    EnsemblFungiiYOR057W; YOR057W; YOR057W.
    GeneIDi854222.
    KEGGisce:YOR057W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U88830 Genomic DNA. Translation: AAB48841.1 .
    Z70678 Genomic DNA. Translation: CAA94542.1 .
    Z74965 Genomic DNA. Translation: CAA99250.1 .
    AY558043 Genomic DNA. Translation: AAS56369.1 .
    BK006948 Genomic DNA. Translation: DAA10836.1 .
    PIRi S66940.
    RefSeqi NP_014700.1. NM_001183476.1.

    3D structure databases

    ProteinModelPortali Q08446.
    SMRi Q08446. Positions 11-77, 92-140, 184-277.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34455. 40 interactions.
    DIPi DIP-1628N.
    IntActi Q08446. 10 interactions.
    MINTi MINT-400978.
    STRINGi 4932.YOR057W.

    Proteomic databases

    MaxQBi Q08446.
    PaxDbi Q08446.
    PeptideAtlasi Q08446.
    PRIDEi Q08446.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR057W ; YOR057W ; YOR057W .
    GeneIDi 854222.
    KEGGi sce:YOR057W.

    Organism-specific databases

    CYGDi YOR057w.
    SGDi S000005583. SGT1.

    Phylogenomic databases

    eggNOGi COG5091.
    GeneTreei ENSGT00390000013700.
    HOGENOMi HOG000141743.
    KOi K12795.
    OMAi ITIYAKN.
    OrthoDBi EOG7RBZMH.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33597-MONOMER.
    Reactomei REACT_189238. The NLRP3 inflammasome.

    Miscellaneous databases

    NextBioi 976089.

    Gene expression databases

    Genevestigatori Q08446.

    Family and domain databases

    Gene3Di 1.25.40.10. 1 hit.
    2.60.40.790. 2 hits.
    InterProi IPR007052. CS_dom.
    IPR008978. HSP20-like_chaperone.
    IPR007699. SGS.
    IPR011990. TPR-like_helical.
    [Graphical view ]
    Pfami PF04969. CS. 1 hit.
    PF05002. SGS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49764. SSF49764. 2 hits.
    PROSITEi PS51203. CS. 1 hit.
    PS51048. SGS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames."
      Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M.
      Yeast 13:379-390(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "SGT1 encodes an essential component of the yeast kinetochore assembly pathway and a novel subunit of the SCF ubiquitin ligase complex."
      Kitagawa K., Skowyra D., Elledge S.J., Harper J.W., Hieter P.
      Mol. Cell 4:21-33(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SKP1, INTERACTION WITH THE SCF COMPLEX, MUTANTS SGT1-3 AND SGT1-5.
    6. "Sgt1p contributes to cyclic AMP pathway activity and physically interacts with the adenylyl cyclase Cyr1p/Cdc35p in budding yeast."
      Dubacq C., Guerois R., Courbeyrette R., Kitagawa K., Mann C.
      Eukaryot. Cell 1:568-582(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CIR1, MUTANT A364A.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168 AND SER-171, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168 AND SER-171, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168 AND SER-171, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSGT1_YEAST
    AccessioniPrimary (citable) accession number: Q08446
    Secondary accession number(s): D6W2C0, E9P8U7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1340 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3