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Protein

Protein SGT1

Gene

SGT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Required for both entry into S phase and kinetochore function. Also involved in cyclic AMP (cAMP) pathway, possibly by participating in the assembly or the conformational activation of specific multiprotein complexes.2 Publications

Miscellaneous

Present with 1340 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • chaperone binding Source: SGD
  • protein binding, bridging Source: SGD

GO - Biological processi

  • cAMP-mediated signaling Source: SGD
  • cell cycle Source: UniProtKB-KW
  • cellular protein-containing complex assembly Source: SGD
  • kinetochore assembly Source: SGD
  • protein-containing complex assembly Source: SGD

Keywordsi

Biological processCell cycle, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-33597-MONOMER
ReactomeiR-SCE-844456 The NLRP3 inflammasome

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SGT12 Publications
Alternative name(s):
Suppressor of G2 allele of SKP11 Publication
Gene namesi
Name:SGT1Imported
Ordered Locus Names:YOR057W
ORF Names:YOR29-08
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR057W
SGDiS000005583 SGT1

Subcellular locationi

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31L → P in sgt1-3; induces arrest of cell division in G2/M; when associated with L-99 and I-213. 1
Mutagenesisi99F → L in sgt1-3; induces arrest of cell division in G2/M; when associated with P-31 I-213. 1
Mutagenesisi213N → I in sgt1-3; induces arrest of cell division in G2/M; when associated with P-31 and L-99. 1
Mutagenesisi220D → V in sgt1-5; induces arrest of cell division in G2/M; when associated with K-364. 1
Mutagenesisi364E → K in sgt1-5; induces arrest of cell division in G2/M; when associated with V-220. 1
Mutagenesisi371S → N in A364a; suppressor of the cdc35-1 allele. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001853931 – 395Protein SGT1Add BLAST395

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei168PhosphoserineCombined sources1
Modified residuei171PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ08446
PaxDbiQ08446
PRIDEiQ08446

PTM databases

iPTMnetiQ08446

Interactioni

Subunit structurei

Interacts with SKP1/CBF3D. Part of SCF E3 ubiquitin ligase complexes containing SKP1, CDC53, HRT1 and some F-box proteins. Interacts with CIR1/CDC35.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • chaperone binding Source: SGD
  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi34455, 659 interactors
DIPiDIP-1628N
IntActiQ08446, 24 interactors
MINTiQ08446
STRINGi4932.YOR057W

Structurei

Secondary structure

1395
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 15Combined sources12
Helixi20 – 33Combined sources14
Helixi38 – 52Combined sources15
Helixi56 – 58Combined sources3
Helixi61 – 79Combined sources19
Helixi84 – 100Combined sources17
Helixi104 – 116Combined sources13
Helixi124 – 133Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AN3X-ray2.82A/B/C1-150[»]
ProteinModelPortaliQ08446
SMRiQ08446
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini182 – 277CSPROSITE-ProRule annotationAdd BLAST96
Domaini312 – 395SGSPROSITE-ProRule annotationAdd BLAST84

Sequence similaritiesi

Belongs to the SGT1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000013700
HOGENOMiHOG000141743
InParanoidiQ08446
KOiK12795
OMAiYYIKRST
OrthoDBiEOG092C5UO0

Family and domain databases

Gene3Di1.25.40.10, 1 hit
2.60.40.790, 1 hit
InterProiView protein in InterPro
IPR007052 CS_dom
IPR008978 HSP20-like_chaperone
IPR007699 SGS_dom
IPR011990 TPR-like_helical_dom_sf
PfamiView protein in Pfam
PF04969 CS, 1 hit
PF05002 SGS, 1 hit
SUPFAMiSSF48452 SSF48452, 1 hit
SSF49764 SSF49764, 2 hits
PROSITEiView protein in PROSITE
PS51203 CS, 1 hit
PS51048 SGS, 1 hit

Sequencei

Sequence statusi: Complete.

Q08446-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVEKDLKTA YKALYDEKEP LKALHLYDEI LKGSPTNLTA LIFKAACLEK
60 70 80 90 100
LYFGFSDWHS DATMENAKEL LDKALMTAEG RGDRSKIGLV NFRYFVHFFN
110 120 130 140 150
IKDYELAQSY FKKAKNLGYV DDTLPLWEDR LETKLNKKNK KQKDSTNKHT
160 170 180 190 200
IKPVESIENR GDNNSSHSPI SPLKIETAPQ ESPKFKIDWY QSSTSVTISL
210 220 230 240 250
FTVNLPESKE QVNIYISPND RRTLSISYQV PKSGSEFQYN AKLSHEVDPK
260 270 280 290 300
AVSLKIFPKK LEITLSKIDS TQWKKLEEDI LTESSRLSDE GKNSDSATRL
310 320 330 340 350
LSAETASKER LSYPSSSKKK IDWSKLDIDE EADEEAGSAD SFFQKLYAGA
360 370 380 390
DPDTKRAMMK SFIESNGTAL STDWEDVSKG TVKTSPPEGM EPKHW
Length:395
Mass (Da):44,860
Last modified:November 1, 1996 - v1
Checksum:i5DE65874BE155552
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13A → T in AAS56369 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88830 Genomic DNA Translation: AAB48841.1
Z70678 Genomic DNA Translation: CAA94542.1
Z74965 Genomic DNA Translation: CAA99250.1
AY558043 Genomic DNA Translation: AAS56369.1
BK006948 Genomic DNA Translation: DAA10836.1
PIRiS66940
RefSeqiNP_014700.1, NM_001183476.1

Genome annotation databases

EnsemblFungiiYOR057W; YOR057W; YOR057W
GeneIDi854222
KEGGisce:YOR057W

Similar proteinsi

Entry informationi

Entry nameiSGT1_YEAST
AccessioniPrimary (citable) accession number: Q08446
Secondary accession number(s): D6W2C0, E9P8U7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health