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Q08446 (SGT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein SGT1
Alternative name(s):
Suppressor of G2 allele of SKP1
Gene names
Name:SGT1
Ordered Locus Names:YOR057W
ORF Names:YOR29-08
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length395 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Required for both entry into S phase and kinetochore function. Also involved in cyclic AMP (cAMP) pathway, possibly by participating in the assembly or the conformational activation of specific multiprotein complexes. Ref.5 Ref.6

Subunit structure

Interacts with SKP1/CBF3D. Part of SCF E3 ubiquitin ligase complexes containing SKP1, CDC53, HRT1 and some F-box proteins. Interacts with CIR1/CDC35. Ref.5 Ref.6

Miscellaneous

Present with 1340 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 CS domain.

Contains 1 SGS domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 395395Protein SGT1
PRO_0000185393

Regions

Domain182 – 27796CS
Domain312 – 39584SGS

Amino acid modifications

Modified residue1681Phosphoserine Ref.8 Ref.9 Ref.10
Modified residue1711Phosphoserine Ref.8 Ref.9 Ref.10

Experimental info

Mutagenesis311L → P in sgt1-3; induces arrest of cell division in G2/M; when associated with L-99 and I-213.
Mutagenesis991F → L in sgt1-3; induces arrest of cell division in G2/M; when associated with P-31 I-213.
Mutagenesis2131N → I in sgt1-3; induces arrest of cell division in G2/M; when associated with P-31 and L-99.
Mutagenesis2201D → V in sgt1-5; induces arrest of cell division in G2/M; when associated with K-364.
Mutagenesis3641E → K in sgt1-5; induces arrest of cell division in G2/M; when associated with V-220.
Mutagenesis3711S → N in A364a; suppressor of the cdc35-1 allele.
Sequence conflict131A → T in AAS56369. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q08446 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 5DE65874BE155552

FASTA39544,860
        10         20         30         40         50         60 
MPVEKDLKTA YKALYDEKEP LKALHLYDEI LKGSPTNLTA LIFKAACLEK LYFGFSDWHS 

        70         80         90        100        110        120 
DATMENAKEL LDKALMTAEG RGDRSKIGLV NFRYFVHFFN IKDYELAQSY FKKAKNLGYV 

       130        140        150        160        170        180 
DDTLPLWEDR LETKLNKKNK KQKDSTNKHT IKPVESIENR GDNNSSHSPI SPLKIETAPQ 

       190        200        210        220        230        240 
ESPKFKIDWY QSSTSVTISL FTVNLPESKE QVNIYISPND RRTLSISYQV PKSGSEFQYN 

       250        260        270        280        290        300 
AKLSHEVDPK AVSLKIFPKK LEITLSKIDS TQWKKLEEDI LTESSRLSDE GKNSDSATRL 

       310        320        330        340        350        360 
LSAETASKER LSYPSSSKKK IDWSKLDIDE EADEEAGSAD SFFQKLYAGA DPDTKRAMMK 

       370        380        390 
SFIESNGTAL STDWEDVSKG TVKTSPPEGM EPKHW 

« Hide

References

« Hide 'large scale' references
[1]"The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames."
Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M.
Yeast 13:379-390(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"SGT1 encodes an essential component of the yeast kinetochore assembly pathway and a novel subunit of the SCF ubiquitin ligase complex."
Kitagawa K., Skowyra D., Elledge S.J., Harper J.W., Hieter P.
Mol. Cell 4:21-33(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SKP1, INTERACTION WITH THE SCF COMPLEX, MUTANTS SGT1-3 AND SGT1-5.
[6]"Sgt1p contributes to cyclic AMP pathway activity and physically interacts with the adenylyl cyclase Cyr1p/Cdc35p in budding yeast."
Dubacq C., Guerois R., Courbeyrette R., Kitagawa K., Mann C.
Eukaryot. Cell 1:568-582(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CIR1, MUTANT A364A.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168 AND SER-171, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168 AND SER-171, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168 AND SER-171, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U88830 Genomic DNA. Translation: AAB48841.1.
Z70678 Genomic DNA. Translation: CAA94542.1.
Z74965 Genomic DNA. Translation: CAA99250.1.
AY558043 Genomic DNA. Translation: AAS56369.1.
BK006948 Genomic DNA. Translation: DAA10836.1.
PIRS66940.
RefSeqNP_014700.1. NM_001183476.1.

3D structure databases

ProteinModelPortalQ08446.
SMRQ08446. Positions 11-77, 92-140, 184-277.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34455. 34 interactions.
DIPDIP-1628N.
IntActQ08446. 10 interactions.
MINTMINT-400978.
STRING4932.YOR057W.

Proteomic databases

MaxQBQ08446.
PaxDbQ08446.
PeptideAtlasQ08446.
PRIDEQ08446.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR057W; YOR057W; YOR057W.
GeneID854222.
KEGGsce:YOR057W.

Organism-specific databases

CYGDYOR057w.
SGDS000005583. SGT1.

Phylogenomic databases

eggNOGCOG5091.
GeneTreeENSGT00390000013700.
HOGENOMHOG000141743.
KOK12795.
OMAITIYAKN.
OrthoDBEOG7RBZMH.

Enzyme and pathway databases

BioCycYEAST:G3O-33597-MONOMER.

Gene expression databases

GenevestigatorQ08446.

Family and domain databases

Gene3D1.25.40.10. 1 hit.
2.60.40.790. 2 hits.
InterProIPR007052. CS_dom.
IPR008978. HSP20-like_chaperone.
IPR007699. SGS.
IPR011990. TPR-like_helical.
[Graphical view]
PfamPF04969. CS. 1 hit.
PF05002. SGS. 1 hit.
[Graphical view]
SUPFAMSSF49764. SSF49764. 2 hits.
PROSITEPS51203. CS. 1 hit.
PS51048. SGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio976089.

Entry information

Entry nameSGT1_YEAST
AccessionPrimary (citable) accession number: Q08446
Secondary accession number(s): D6W2C0, E9P8U7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families