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Protein

Protein SGT1

Gene

SGT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Required for both entry into S phase and kinetochore function. Also involved in cyclic AMP (cAMP) pathway, possibly by participating in the assembly or the conformational activation of specific multiprotein complexes.2 Publications

GO - Molecular functioni

  • chaperone binding Source: SGD
  • protein binding, bridging Source: SGD

GO - Biological processi

  • cAMP-mediated signaling Source: SGD
  • cell cycle Source: UniProtKB-KW
  • kinetochore assembly Source: SGD
  • protein complex assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-33597-MONOMER.
ReactomeiR-SCE-844456. The NLRP3 inflammasome.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SGT12 Publications
Alternative name(s):
Suppressor of G2 allele of SKP11 Publication
Gene namesi
Name:SGT1Imported
Ordered Locus Names:YOR057W
ORF Names:YOR29-08
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR057W.
SGDiS000005583. SGT1.

Subcellular locationi

GO - Cellular componenti

  • ubiquitin ligase complex Source: SGD
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31L → P in sgt1-3; induces arrest of cell division in G2/M; when associated with L-99 and I-213. 1
Mutagenesisi99F → L in sgt1-3; induces arrest of cell division in G2/M; when associated with P-31 I-213. 1
Mutagenesisi213N → I in sgt1-3; induces arrest of cell division in G2/M; when associated with P-31 and L-99. 1
Mutagenesisi220D → V in sgt1-5; induces arrest of cell division in G2/M; when associated with K-364. 1
Mutagenesisi364E → K in sgt1-5; induces arrest of cell division in G2/M; when associated with V-220. 1
Mutagenesisi371S → N in A364a; suppressor of the cdc35-1 allele. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001853931 – 395Protein SGT1Add BLAST395

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei168PhosphoserineCombined sources1
Modified residuei171PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ08446.
PRIDEiQ08446.

PTM databases

iPTMnetiQ08446.

Interactioni

Subunit structurei

Interacts with SKP1/CBF3D. Part of SCF E3 ubiquitin ligase complexes containing SKP1, CDC53, HRT1 and some F-box proteins. Interacts with CIR1/CDC35.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CTF13P352033EBI-17070,EBI-4085
HSP82P028292EBI-17070,EBI-8659
SKP1P522866EBI-17070,EBI-4090

GO - Molecular functioni

  • chaperone binding Source: SGD
  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi34455. 41 interactors.
DIPiDIP-1628N.
IntActiQ08446. 10 interactors.
MINTiMINT-400978.

Structurei

3D structure databases

ProteinModelPortaliQ08446.
SMRiQ08446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini182 – 277CSPROSITE-ProRule annotationAdd BLAST96
Domaini312 – 395SGSPROSITE-ProRule annotationAdd BLAST84

Sequence similaritiesi

Belongs to the SGT1 family.Curated
Contains 1 CS domain.PROSITE-ProRule annotation
Contains 1 SGS domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000013700.
HOGENOMiHOG000141743.
InParanoidiQ08446.
KOiK12795.
OMAiIITIYAK.
OrthoDBiEOG092C5UO0.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
2.60.40.790. 2 hits.
InterProiIPR007052. CS_dom.
IPR008978. HSP20-like_chaperone.
IPR007699. SGS_dom.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF04969. CS. 1 hit.
PF05002. SGS. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
SSF49764. SSF49764. 2 hits.
PROSITEiPS51203. CS. 1 hit.
PS51048. SGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08446-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVEKDLKTA YKALYDEKEP LKALHLYDEI LKGSPTNLTA LIFKAACLEK
60 70 80 90 100
LYFGFSDWHS DATMENAKEL LDKALMTAEG RGDRSKIGLV NFRYFVHFFN
110 120 130 140 150
IKDYELAQSY FKKAKNLGYV DDTLPLWEDR LETKLNKKNK KQKDSTNKHT
160 170 180 190 200
IKPVESIENR GDNNSSHSPI SPLKIETAPQ ESPKFKIDWY QSSTSVTISL
210 220 230 240 250
FTVNLPESKE QVNIYISPND RRTLSISYQV PKSGSEFQYN AKLSHEVDPK
260 270 280 290 300
AVSLKIFPKK LEITLSKIDS TQWKKLEEDI LTESSRLSDE GKNSDSATRL
310 320 330 340 350
LSAETASKER LSYPSSSKKK IDWSKLDIDE EADEEAGSAD SFFQKLYAGA
360 370 380 390
DPDTKRAMMK SFIESNGTAL STDWEDVSKG TVKTSPPEGM EPKHW
Length:395
Mass (Da):44,860
Last modified:November 1, 1996 - v1
Checksum:i5DE65874BE155552
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13A → T in AAS56369 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88830 Genomic DNA. Translation: AAB48841.1.
Z70678 Genomic DNA. Translation: CAA94542.1.
Z74965 Genomic DNA. Translation: CAA99250.1.
AY558043 Genomic DNA. Translation: AAS56369.1.
BK006948 Genomic DNA. Translation: DAA10836.1.
PIRiS66940.
RefSeqiNP_014700.1. NM_001183476.1.

Genome annotation databases

EnsemblFungiiYOR057W; YOR057W; YOR057W.
GeneIDi854222.
KEGGisce:YOR057W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88830 Genomic DNA. Translation: AAB48841.1.
Z70678 Genomic DNA. Translation: CAA94542.1.
Z74965 Genomic DNA. Translation: CAA99250.1.
AY558043 Genomic DNA. Translation: AAS56369.1.
BK006948 Genomic DNA. Translation: DAA10836.1.
PIRiS66940.
RefSeqiNP_014700.1. NM_001183476.1.

3D structure databases

ProteinModelPortaliQ08446.
SMRiQ08446.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34455. 41 interactors.
DIPiDIP-1628N.
IntActiQ08446. 10 interactors.
MINTiMINT-400978.

PTM databases

iPTMnetiQ08446.

Proteomic databases

MaxQBiQ08446.
PRIDEiQ08446.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR057W; YOR057W; YOR057W.
GeneIDi854222.
KEGGisce:YOR057W.

Organism-specific databases

EuPathDBiFungiDB:YOR057W.
SGDiS000005583. SGT1.

Phylogenomic databases

GeneTreeiENSGT00390000013700.
HOGENOMiHOG000141743.
InParanoidiQ08446.
KOiK12795.
OMAiIITIYAK.
OrthoDBiEOG092C5UO0.

Enzyme and pathway databases

BioCyciYEAST:G3O-33597-MONOMER.
ReactomeiR-SCE-844456. The NLRP3 inflammasome.

Miscellaneous databases

PROiQ08446.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
2.60.40.790. 2 hits.
InterProiIPR007052. CS_dom.
IPR008978. HSP20-like_chaperone.
IPR007699. SGS_dom.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF04969. CS. 1 hit.
PF05002. SGS. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
SSF49764. SSF49764. 2 hits.
PROSITEiPS51203. CS. 1 hit.
PS51048. SGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSGT1_YEAST
AccessioniPrimary (citable) accession number: Q08446
Secondary accession number(s): D6W2C0, E9P8U7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1340 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.