Protein SGT1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q08446 (SGT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 108. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein SGT1

Alternative name(s):
Suppressor of G2 allele of SKP1

Gene names

Name:	SGT1
Ordered Locus Names:	YOR057W
ORF Names:	YOR29-08

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Required for both entry into S phase and kinetochore function. Also involved in cyclic AMP (cAMP) pathway, possibly by participating in the assembly or the conformational activation of specific multiprotein complexes. Ref.5 Ref.6
Subunit structure	Interacts with SKP1/CBF3D. Part of SCF E3 ubiquitin ligase complexes containing SKP1, CDC53, HRT1 and some F-box proteins. Interacts with CIR1/CDC35. Ref.5 Ref.6
Miscellaneous	Present with 1340 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 CS domain. Contains 1 SGS domain.

Ontologies

Keywords
Biological process	Cell cycle Ubl conjugation pathway
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cAMP-mediated signaling Inferred from genetic interaction Ref.6. Source: SGD cell cycle Inferred from electronic annotation. Source: UniProtKB-KW kinetochore assembly Inferred from direct assay Ref.5. Source: SGD protein ubiquitination Traceable author statement Ref.6. Source: SGD regulation of cell cycle Traceable author statement PubMed 11847307. Source: SGD
Cellular_component	ubiquitin ligase complex Inferred from direct assay Ref.5. Source: SGD
Molecular_function	chaperone binding Inferred from direct assay PubMed 16945921. Source: SGD protein binding, bridging Inferred from direct assay PubMed 16945921. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CTF13	P35203	3	EBI-17070,EBI-4085
SKP1	P52286	7	EBI-17070,EBI-4090

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 395

395

Protein SGT1

PRO_0000185393

Regions

Domain

182 – 277

Domain

312 – 395

SGS

Amino acid modifications

Modified residue

165

Phosphoserine Ref.9 Ref.10

Modified residue

166

Phosphoserine Ref.10

Modified residue

168

Phosphoserine Ref.8 Ref.9 Ref.10

Modified residue

171

Phosphoserine Ref.8 Ref.9 Ref.10

Modified residue

225

Phosphoserine Ref.10

Modified residue

305

Phosphothreonine Ref.10

Experimental info

Mutagenesis

L → P in sgt1-3; induces arrest of cell division in G2/M; when associated with L-99 and I-213.

Mutagenesis

F → L in sgt1-3; induces arrest of cell division in G2/M; when associated with P-31 I-213.

Mutagenesis

213

N → I in sgt1-3; induces arrest of cell division in G2/M; when associated with P-31 and L-99.

Mutagenesis

220

D → V in sgt1-5; induces arrest of cell division in G2/M; when associated with K-364.

Mutagenesis

364

E → K in sgt1-5; induces arrest of cell division in G2/M; when associated with V-220.

Mutagenesis

371

S → N in A364a; suppressor of the cdc35-1 allele.

Sequence conflict

A → T in AAS56369. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

Q08446 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 5DE65874BE155552
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FASTA

395

44,860

        10         20         30         40         50         60 
MPVEKDLKTA YKALYDEKEP LKALHLYDEI LKGSPTNLTA LIFKAACLEK LYFGFSDWHS 

        70         80         90        100        110        120 
DATMENAKEL LDKALMTAEG RGDRSKIGLV NFRYFVHFFN IKDYELAQSY FKKAKNLGYV 

       130        140        150        160        170        180 
DDTLPLWEDR LETKLNKKNK KQKDSTNKHT IKPVESIENR GDNNSSHSPI SPLKIETAPQ 

       190        200        210        220        230        240 
ESPKFKIDWY QSSTSVTISL FTVNLPESKE QVNIYISPND RRTLSISYQV PKSGSEFQYN 

       250        260        270        280        290        300 
AKLSHEVDPK AVSLKIFPKK LEITLSKIDS TQWKKLEEDI LTESSRLSDE GKNSDSATRL 

       310        320        330        340        350        360 
LSAETASKER LSYPSSSKKK IDWSKLDIDE EADEEAGSAD SFFQKLYAGA DPDTKRAMMK 

       370        380        390 
SFIESNGTAL STDWEDVSKG TVKTSPPEGM EPKHW

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames." Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M. Yeast 13:379-390(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"SGT1 encodes an essential component of the yeast kinetochore assembly pathway and a novel subunit of the SCF ubiquitin ligase complex." Kitagawa K., Skowyra D., Elledge S.J., Harper J.W., Hieter P. Mol. Cell 4:21-33(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SKP1, INTERACTION WITH THE SCF COMPLEX, MUTANTS SGT1-3 AND SGT1-5.
[6]	"Sgt1p contributes to cyclic AMP pathway activity and physically interacts with the adenylyl cyclase Cyr1p/Cdc35p in budding yeast." Dubacq C., Guerois R., Courbeyrette R., Kitagawa K., Mann C. Eukaryot. Cell 1:568-582(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CIR1, MUTANT A364A.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168 AND SER-171, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165; SER-168 AND SER-171, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165; SER-166; SER-168; SER-171; SER-225 AND THR-305, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U88830 Genomic DNA. Translation: AAB48841.1. Z70678 Genomic DNA. Translation: CAA94542.1. Z74965 Genomic DNA. Translation: CAA99250.1. AY558043 Genomic DNA. Translation: AAS56369.1. BK006948 Genomic DNA. Translation: DAA10836.1.
PIR	S66940.
RefSeq	NP_014700.1. NM_001183476.1.
3D structure databases
ProteinModelPortal	Q08446.
SMR	Q08446. Positions 184-277.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1628N.
IntAct	Q08446. 9 interactions.
MINT	MINT-400978.
STRING	4932.YOR057W.
Proteomic databases
PaxDb	Q08446.
PeptideAtlas	Q08446.
PRIDE	Q08446.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR057W; YOR057W; YOR057W.
GeneID	854222.
KEGG	sce:YOR057W.
Organism-specific databases
CYGD	YOR057w.
SGD	S000005583. SGT1.
Phylogenomic databases
eggNOG	COG5091.
GeneTree	ENSGT00390000013700.
HOGENOM	HOG000141743.
KO	K12795.
OMA	AMMKSFI.
OrthoDB	EOG4RJK9T.
Gene expression databases
Genevestigator	Q08446.
GermOnline	YOR057W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.25.40.10. 1 hit.
InterPro	IPR007052. CS-like_domain. IPR017447. CS_domain. IPR008978. HSP20-like_chaperone. IPR007699. SGS. IPR011990. TPR-like_helical. [Graphical view]
Pfam	PF04969. CS. 1 hit. PF05002. SGS. 1 hit. [Graphical view]
SUPFAM	SSF49764. HSP20_chap. 1 hit.
PROSITE	PS51203. CS. 1 hit. PS51048. SGS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976089.

Entry information

Entry name

SGT1_YEAST

Accession

Primary (citable) accession number: Q08446
Secondary accession number(s): D6W2C0, E9P8U7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents