Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

20S-pre-rRNA D-site endonuclease NOB1

Gene

NOB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the synthesis of 40S ribosome subunits. Has a role in processing 20S pre-rRNA into the mature 18S rRNA, where it is required for cleavage at the 3' end of the mature 18S rRNA (D-site). Accompanies the 20S pre-rRNA from the nucleus to the cytoplasm. In association with NIN1, may promote the recruitment of the proteasome to the ribosomal subunits stalled in maturation.2 Publications

Miscellaneous

Present with 4490 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi304ZincBy similarity1
Metal bindingi307ZincBy similarity1
Metal bindingi323ZincBy similarity1
Metal bindingi326ZincBy similarity1

GO - Molecular functioni

  • endoribonuclease activity Source: SGD
  • metal ion binding Source: UniProtKB-KW
  • small ribosomal subunit rRNA binding Source: SGD

GO - Biological processi

  • cleavage involved in rRNA processing Source: GO_Central
  • maturation of SSU-rRNA Source: SGD
  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • proteasome assembly Source: SGD
  • ribosomal small subunit biogenesis Source: MGI

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processRibosome biogenesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33596-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
20S-pre-rRNA D-site endonuclease NOB1 (EC:3.1.-.-)
Alternative name(s):
NIN1-binding protein
Pre-rRNA-processing endonuclease NOB1
Gene namesi
Name:NOB1
Ordered Locus Names:YOR056C
ORF Names:YOR29-07
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR056C
SGDiS000005582 NOB1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi92D → N: No 20S cleavage. 1 Publication1
Mutagenesisi110D → N: No change in activity or growth. 1 Publication1
Mutagenesisi279L → R: Temperature-sensitive. 1 Publication1
Mutagenesisi280Q → G: Temperature-sensitive. 1 Publication1
Mutagenesisi281M → G: Temperature-sensitive. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002705541 – 45920S-pre-rRNA D-site endonuclease NOB1Add BLAST459

Proteomic databases

MaxQBiQ08444
PaxDbiQ08444
PRIDEiQ08444

PTM databases

iPTMnetiQ08444

Interactioni

Subunit structurei

Component of the small ribosomal subunit, ribosomal RNA processing complex (SSU RRP complex). Interacts with PNO1.2 Publications

Protein-protein interaction databases

BioGridi34454, 226 interactors
DIPiDIP-6440N
IntActiQ08444, 35 interactors
MINTiQ08444
STRINGi4932.YOR056C

Structurei

3D structure databases

ProteinModelPortaliQ08444
SMRiQ08444
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 115PINcAdd BLAST106

Sequence similaritiesi

Belongs to the NOB1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000015857
HOGENOMiHOG000159692
InParanoidiQ08444
KOiK11883
OMAiPHLTEDQ
OrthoDBiEOG092C221B

Family and domain databases

Gene3Di3.30.40.120, 1 hit
InterProiView protein in InterPro
IPR017117 Nob1_euk
IPR036283 NOB1_Zf-like_sf
IPR014881 NOB1_Zn-bd
IPR002716 PIN_dom
IPR033411 Ribonuclease_PIN
PfamiView protein in Pfam
PF08772 NOB1_Zn_bind, 1 hit
PF17146 PIN_6, 1 hit
PIRSFiPIRSF037125 D-site_20S_pre-rRNA_nuclease, 1 hit
SMARTiView protein in SMART
SM00670 PINc, 1 hit
SUPFAMiSSF144206 SSF144206, 1 hit

Sequencei

Sequence statusi: Complete.

Q08444-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTENQTAHVR ALILDATPLI TQSYTHYQNY AQSFYTTPTV FQEIKDAQAR
60 70 80 90 100
KNLEIWQSLG TLKLVHPSEN SIAKVSTFAK LTGDYSVLSA NDLHILALTY
110 120 130 140 150
ELEIKLNNGD WRLRKKPGDA LDASKADVGT DGKQKLTEDN KKEEDSESVP
160 170 180 190 200
KKKNKRRGGK KQKAKREARE AREAENANLE LESKAEEHVE EAGSKEQICN
210 220 230 240 250
DENIKESSDL NEVFEDADDD GDWITPENLT EAIIKDSGED TTGSLGVEAS
260 270 280 290 300
EEDRHVALNR PENQVALATG DFAVQNVALQ MNLNLMNFMS GLKIKRIRNY
310 320 330 340 350
MLRCHACFKI FPLPKDGKPK HFCASCGGQG TLLRCAVSVD SRTGNVTPHL
360 370 380 390 400
KSNFQWNNRG NRYSVASPLS KNSQKRYGKK GHVHSKPQEN VILREDQKEY
410 420 430 440 450
EKVIKQEEWT RRHNEKILNN WIGGGSADNY ISPFAITGLK QHNVRIGKGR

YVNSSKRRS
Length:459
Mass (Da):51,742
Last modified:November 1, 1996 - v1
Checksum:i96DEB818BB490A98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70678 Genomic DNA Translation: CAA94541.1
Z74964 Genomic DNA Translation: CAA99249.1
BK006948 Genomic DNA Translation: DAA10835.1
PIRiS66939
RefSeqiNP_014699.1, NM_001183475.1

Genome annotation databases

EnsemblFungiiYOR056C; YOR056C; YOR056C
GeneIDi854221
KEGGisce:YOR056C

Similar proteinsi

Entry informationi

Entry nameiNOB1_YEAST
AccessioniPrimary (citable) accession number: Q08444
Secondary accession number(s): D6W2B9, O00021
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health