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Protein

20S-pre-rRNA D-site endonuclease NOB1

Gene

NOB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the synthesis of 40S ribosome subunits. Has a role in processing 20S pre-rRNA into the mature 18S rRNA, where it is required for cleavage at the 3' end of the mature 18S rRNA (D-site). Accompanies the 20S pre-rRNA from the nucleus to the cytoplasm. In association with NIN1, may promote the recruitment of the proteasome to the ribosomal subunits stalled in maturation.2 Publications

Miscellaneous

Present with 4490 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi304ZincBy similarity1
Metal bindingi307ZincBy similarity1
Metal bindingi323ZincBy similarity1
Metal bindingi326ZincBy similarity1

GO - Molecular functioni

  • endoribonuclease activity Source: SGD
  • metal ion binding Source: UniProtKB-KW
  • small ribosomal subunit rRNA binding Source: SGD

GO - Biological processi

  • cleavage involved in rRNA processing Source: GO_Central
  • maturation of SSU-rRNA Source: SGD
  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • proteasome assembly Source: SGD
  • ribosomal small subunit biogenesis Source: MGI

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processRibosome biogenesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33596-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
20S-pre-rRNA D-site endonuclease NOB1 (EC:3.1.-.-)
Alternative name(s):
NIN1-binding protein
Pre-rRNA-processing endonuclease NOB1
Gene namesi
Name:NOB1
Ordered Locus Names:YOR056C
ORF Names:YOR29-07
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR056C.
SGDiS000005582. NOB1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi92D → N: No 20S cleavage. 1 Publication1
Mutagenesisi110D → N: No change in activity or growth. 1 Publication1
Mutagenesisi279L → R: Temperature-sensitive. 1 Publication1
Mutagenesisi280Q → G: Temperature-sensitive. 1 Publication1
Mutagenesisi281M → G: Temperature-sensitive. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002705541 – 45920S-pre-rRNA D-site endonuclease NOB1Add BLAST459

Proteomic databases

MaxQBiQ08444.
PRIDEiQ08444.

PTM databases

iPTMnetiQ08444.

Interactioni

Subunit structurei

Component of the small ribosomal subunit, ribosomal RNA processing complex (SSU RRP complex). Interacts with PNO1.2 Publications

Protein-protein interaction databases

BioGridi34454. 222 interactors.
DIPiDIP-6440N.
IntActiQ08444. 35 interactors.
MINTiMINT-621960.
STRINGi4932.YOR056C.

Structurei

3D structure databases

ProteinModelPortaliQ08444.
SMRiQ08444.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 115PINcAdd BLAST106

Sequence similaritiesi

Belongs to the NOB1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000015857.
HOGENOMiHOG000159692.
InParanoidiQ08444.
KOiK11883.
OMAiPHLTEDQ.
OrthoDBiEOG092C221B.

Family and domain databases

Gene3Di3.40.50.1010. 1 hit.
InterProiView protein in InterPro
IPR017117. Nob1_euk.
IPR036283. NOB1_Zf-like_sf.
IPR014881. NOB1_Zn-bd.
IPR002716. PIN_dom.
IPR029060. PIN_domain-like.
IPR033411. Ribonuclease_PIN.
PfamiView protein in Pfam
PF08772. NOB1_Zn_bind. 1 hit.
PF17146. PIN_6. 1 hit.
PIRSFiPIRSF037125. D-site_20S_pre-rRNA_nuclease. 1 hit.
SMARTiView protein in SMART
SM00670. PINc. 1 hit.
SUPFAMiSSF144206. SSF144206. 1 hit.

Sequencei

Sequence statusi: Complete.

Q08444-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTENQTAHVR ALILDATPLI TQSYTHYQNY AQSFYTTPTV FQEIKDAQAR
60 70 80 90 100
KNLEIWQSLG TLKLVHPSEN SIAKVSTFAK LTGDYSVLSA NDLHILALTY
110 120 130 140 150
ELEIKLNNGD WRLRKKPGDA LDASKADVGT DGKQKLTEDN KKEEDSESVP
160 170 180 190 200
KKKNKRRGGK KQKAKREARE AREAENANLE LESKAEEHVE EAGSKEQICN
210 220 230 240 250
DENIKESSDL NEVFEDADDD GDWITPENLT EAIIKDSGED TTGSLGVEAS
260 270 280 290 300
EEDRHVALNR PENQVALATG DFAVQNVALQ MNLNLMNFMS GLKIKRIRNY
310 320 330 340 350
MLRCHACFKI FPLPKDGKPK HFCASCGGQG TLLRCAVSVD SRTGNVTPHL
360 370 380 390 400
KSNFQWNNRG NRYSVASPLS KNSQKRYGKK GHVHSKPQEN VILREDQKEY
410 420 430 440 450
EKVIKQEEWT RRHNEKILNN WIGGGSADNY ISPFAITGLK QHNVRIGKGR

YVNSSKRRS
Length:459
Mass (Da):51,742
Last modified:November 1, 1996 - v1
Checksum:i96DEB818BB490A98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70678 Genomic DNA. Translation: CAA94541.1.
Z74964 Genomic DNA. Translation: CAA99249.1.
BK006948 Genomic DNA. Translation: DAA10835.1.
PIRiS66939.
RefSeqiNP_014699.1. NM_001183475.1.

Genome annotation databases

EnsemblFungiiYOR056C; YOR056C; YOR056C.
GeneIDi854221.
KEGGisce:YOR056C.

Similar proteinsi

Entry informationi

Entry nameiNOB1_YEAST
AccessioniPrimary (citable) accession number: Q08444
Secondary accession number(s): D6W2B9, O00021
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: October 25, 2017
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names