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Q08436 (PMA3_NICPL) Reviewed, UniProtKB/Swiss-Prot

Last modified October 19, 2011. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Plasma membrane ATPase 3
EC=3.6.3.6
Alternative name(s):
Proton pump 3
Gene names
Name:PMA3
OrganismNicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco)
Taxonomic identifier4092 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length956 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Catalytic activity

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Expressed in roots, stems, leaves from both vegetative and flowering plants, and flowers at early and late stages of development with highest expression levels found in flowers and root tissue.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 956956Plasma membrane ATPase 3
PRO_0000046292

Regions

Topological domain1 – 6565Cytoplasmic Potential
Transmembrane66 – 8520Helical; Name=1; Potential
Topological domain86 – 9712Extracellular Potential
Transmembrane98 – 11821Helical; Name=2; Potential
Topological domain119 – 247129Cytoplasmic Potential
Transmembrane248 – 26821Helical; Name=3; Potential
Topological domain269 – 27810Extracellular Potential
Transmembrane279 – 30022Helical; Name=4; Potential
Topological domain301 – 647347Cytoplasmic Potential
Transmembrane648 – 66922Helical; Name=5; Potential
Topological domain670 – 6745Extracellular Potential
Transmembrane675 – 69723Helical; Name=6; Potential
Topological domain698 – 71316Cytoplasmic Potential
Transmembrane714 – 73421Helical; Name=7; Potential
Topological domain735 – 75925Extracellular Potential
Transmembrane760 – 78021Helical; Name=8; Potential
Topological domain781 – 79212Cytoplasmic Potential
Transmembrane793 – 81321Helical; Name=9; Potential
Topological domain814 – 8229Extracellular Potential
Transmembrane823 – 84321Helical; Name=10; Potential
Topological domain844 – 956113Cytoplasmic Potential

Sites

Active site33314-aspartylphosphate intermediate By similarity
Metal binding5921Magnesium By similarity
Metal binding5961Magnesium By similarity

Natural variations

Natural variant631S → L.

Secondary structure

1 ..... 956
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q08436 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 94916371CB434BA4

FASTA956105,116
        10         20         30         40         50         60 
MGEKPEVLDA VLKETVDLEN IPIEEVFENL RCTKEGLTAT AAQERLSIFG YNKLEEKKES 

        70         80         90        100        110        120 
KFSKFLGFMW NPLSWVMEAA AIMAIALANG GGKPPDWQDF VGIITLLIIN STISFIEENN 

       130        140        150        160        170        180 
AGNAAAALMA RLAPKAKVLR DGKWKEEDAA VLVPGDIISI KLGDIIPADA RLLEGDPLKI 

       190        200        210        220        230        240 
DQSALTGESL PVTKGPGDGV YSGSTCKQGE IEAVVIATGV HTFFGKAAHL VDSTNQVGHF 

       250        260        270        280        290        300 
QKVLTAIGNF CICSIAVGMI IEIIVMYPIQ HRKYRPGIDN LLVLLIGGIP IAMPTVLSVT 

       310        320        330        340        350        360 
MAIGSHRLAQ QGAITKRMTA IEEMAGMDVL CSDKTGTLTL NKLTVDKYLI EVFARGVDAD 

       370        380        390        400        410        420 
TVVLMAARAS RTENQDAIDA AIVGMLADPK EARAGIREIH FLPFNPTDKR TALTYLDGEG 

       430        440        450        460        470        480 
KMHRVSKGAP EQILHLAHNK SDIERRVHAV IDKFAERGLR SLAVAYQEVP EGRKESAGGP 

       490        500        510        520        530        540 
WQFIALLPLF DPPRHDSAET IRRALNLGVN VKMITGDQLA IGKETGRRLG MGTNMYPSSA 

       550        560        570        580        590        600 
LLGQTKDESI SALPVDELIE KADGFAGVFP EHKYEIVKRL QARKHICGMT GDGVNDAPAL 

       610        620        630        640        650        660 
KKADIGIAVD DATDAARSAS DIVLTEPGLS VIISAVLTSR AIFQRMKNYT IYAVSITIRI 

       670        680        690        700        710        720 
VLGFMLLALI WQFDFPPFMV LIIAILNDGT IMTISKDRVK PSPLPDSWKL AEIFTTGVVL 

       730        740        750        760        770        780 
GGYLAMMTVI FFWAAYKTNF FPRVFGVSTL EKTATDDFRK LASAIYLQVS TISQALIFVT 

       790        800        810        820        830        840 
RSRSWSFMER PGLLLVVAFF IAQLVATLIA VYANWSFAAI EGIGWGWAGV IWLYNIVFYI 

       850        860        870        880        890        900 
PLDLXXFLIR YALSGKAWDL VIEQRIAFTR KKDFGKEQRE LQWAHAQRTL HGLQVPDPKI 

       910        920        930        940        950 
FSETTNFNEL NQLAEEAKRR AEIARLRELH TLKGHVESVV KLKGLDIETI QQAYTV

« Hide

References

[1]"Differential expression within a three-gene subfamily encoding a plasma membrane H(+)-ATPase in Nicotiana plumbaginifolia."
Perez C., Michelet B., Ferrant V., Bogaerts P., Boutry M.
J. Biol. Chem. 267:1204-1211(1992) [PubMed: 1530935] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Tissue: Root.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M80490 mRNA. Translation: AAA34098.1.
M80491 Genomic DNA. Translation: AAA34099.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2O98X-ray2.70P/Q905-956[»]
ProteinModelPortalQ08436.
SMRQ08436. Positions 905-956.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR008250. ATPase_P-typ_ATPase-assoc-dom.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023300. ATPase_P-typ_cyto_domA.
IPR023299. ATPase_P-typ_cyto_domN.
IPR000695. ATPase_P-typ_H-transp.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR006534. ATPase_P-typ_PM_proton-efflux.
IPR023298. ATPase_P-typ_TM_dom.
IPR005834. Dehalogen-like_hydro.
IPR023214. HAD-like_dom.
[Graphical view]
Gene3DG3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 2 hits.
G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit.
G3DSA:1.20.1110.10. ATPase_P-typ_TM_dom. 3 hits.
PANTHERPTHR24093:SF61. PTHR24093:SF61. 1 hit.
PfamPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
PR00120. HATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF56784. HAD-like_dom. 1 hit.
TIGRFAMsTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePMA3_NICPL
AccessionPrimary (citable) accession number: Q08436
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 19, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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