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Q08435 (PMA1_NICPL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Plasma membrane ATPase 1
EC=3.6.3.6
Alternative name(s):
Proton pump 1
Gene names
Name:PMA1
OrganismNicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco)
Taxonomic identifier4092 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length957 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Catalytic activity

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Expressed in roots, stems, leaves from both vegetative and flowering plants, and flowers at early and late stages of development with highest expression levels found in flowers and stem.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 957957Plasma membrane ATPase 1
PRO_0000046291

Regions

Topological domain1 – 6666Cytoplasmic Potential
Transmembrane67 – 8620Helical; Name=1; Potential
Topological domain87 – 9812Extracellular Potential
Transmembrane99 – 11921Helical; Name=2; Potential
Topological domain120 – 248129Cytoplasmic Potential
Transmembrane249 – 26921Helical; Name=3; Potential
Topological domain270 – 27910Extracellular Potential
Transmembrane280 – 30122Helical; Name=4; Potential
Topological domain302 – 648347Cytoplasmic Potential
Transmembrane649 – 67022Helical; Name=5; Potential
Topological domain671 – 6755Extracellular Potential
Transmembrane676 – 69823Helical; Name=6; Potential
Topological domain699 – 71416Cytoplasmic Potential
Transmembrane715 – 73521Helical; Name=7; Potential
Topological domain736 – 76025Extracellular Potential
Transmembrane761 – 78121Helical; Name=8; Potential
Topological domain782 – 79312Cytoplasmic Potential
Transmembrane794 – 81421Helical; Name=9; Potential
Topological domain815 – 8239Extracellular Potential
Transmembrane824 – 84421Helical; Name=10; Potential
Topological domain845 – 957113Cytoplasmic Potential

Sites

Active site33414-aspartylphosphate intermediate By similarity
Metal binding5931Magnesium By similarity
Metal binding5971Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q08435 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 1A2B0F8A5420BEB1

FASTA957105,155
        10         20         30         40         50         60 
MGEEKPEVLD AVLKEAVDLE NIPIEEVFEN LRCTKEGLTA TAAQERLAIF GYNKLEEKKD 

        70         80         90        100        110        120 
SKLLKFLGFM WNPLSWVMEA AAIMAIALAN GGGKPPDWQD FVGIITLLII NSTISFIEEN 

       130        140        150        160        170        180 
NAGNAAAALM ARLAPKAKVL RDGRWKEEDA AVLVPGDIIS IKLGDIIPAD ARLLEGDPLK 

       190        200        210        220        230        240 
IDQSALTGES LPVTKGPGDG VYSGSTCKQG EIEAIVIATG VHTFFGKAAH LVDSTNQVGH 

       250        260        270        280        290        300 
FQKVLTAIGN FCICSIAVGM IIEIIVMYPI QHRAYRPGID NLLVLLIGGI PIAMPTVLSV 

       310        320        330        340        350        360 
TMAIGSHRLA QQGAITKRMT AIEEMAGMDV LCSDKTGTLT LNKLTVDKNL IEVFAKGVDA 

       370        380        390        400        410        420 
DMVVLMAARA SRTENQDAID AAIVGMLADP KEARAGIREI HFLPFNPTDK RTALTYLDGE 

       430        440        450        460        470        480 
GKMHRVSKGA PEQILNLAHN KSDIERRVHA VIDKFAERGL RSLGVAYQEV PEGRKESAGG 

       490        500        510        520        530        540 
PWQFIGLLPL FDPPRHDSAE TIRRALNLGV NVKMVTGDQL AIGKETGRRL GMGTNMYPSS 

       550        560        570        580        590        600 
ALLGQTKDES ISALPIDELI EKADGFAGVF PEHKYEIVKR LQARKHICGM TGDGVNDAPA 

       610        620        630        640        650        660 
LKKADIGIAV DDATDAARSA SDIVLTEPGL SVIISAVLTS RAIFQRMKNY TIYAVSITIR 

       670        680        690        700        710        720 
IVLGFMLLAL IWKFDFPPFM VLIIAILNDG TIMTISKDRV KPSPLPDSWK LAEIFTTGIV 

       730        740        750        760        770        780 
LGGYLAMMTV IFFWAAYKTN FFPHVFGVST LEKTATDDFR KLASAIYLQV SIISQALIFV 

       790        800        810        820        830        840 
TRSRSWSFVE RPGFLLVIAF VIAQLVATLI AVYANWSFAA IEGIGWGWAG VIWIYNLVFY 

       850        860        870        880        890        900 
IPLDIIKFFI RYALSGRAWD LVFERRIAFT RKKDFGKEQR ELQWAHAQRT LHGLQVPDTK 

       910        920        930        940        950 
LFSEATNFNE LNQLAEEAKR RAEIARLREL HTLKGHVESV VKLKGLDIET IQQAYTV

« Hide

References

[1]"Differential expression within a three-gene subfamily encoding a plasma membrane H(+)-ATPase in Nicotiana plumbaginifolia."
Perez C., Michelet B., Ferrant V., Bogaerts P., Boutry M.
J. Biol. Chem. 267:1204-1211(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Root.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M80489 Genomic DNA. Translation: AAA34094.1.
PIRA41779.

3D structure databases

ProteinModelPortalQ08435.
SMRQ08435. Positions 17-852, 906-957.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR006534. H+_ATPase_P-typ_IIIA.
IPR023214. HAD-like_dom.
[Graphical view]
PANTHERPTHR24093. PTHR24093. 1 hit.
PfamPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
PR00120. HATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF56784. HAD-like_dom. 1 hit.
TIGRFAMsTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePMA1_NICPL
AccessionPrimary (citable) accession number: Q08435
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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