Skip Header

Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q08432 (CBL_BACSU)

Last modified January 19, 2010. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Cystathionine beta-lyase patB
      Short name=CBL
    EC=4.4.1.8
Alternative name(s):
    Beta-cystathionase
    Cysteine lyase
Gene names
Name: patB
Ordered Locus Names: BSU31440
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Hide | Top

Protein attributes

Sequence length387 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Catalyzes the transformation of cystathionine to homocysteine. Also exhibits cysteine desulfhydrase activity in vitro, producing sulfide from cysteine.

Catalytic activity

L-cystathionine + H2O = L-homocysteine + NH3 + pyruvate. Ref.4

Cofactor

Pyridoxal phosphate By similarity.

Induction

Constitutively expressed at a low level. Ref.4

Disruption phenotype

No visible phenotype. Ref.4

Sequence similarities

Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. MalY/patB cystathionine beta-lyase subfamily.

Biophysicochemical properties

Kinetic parameters:

KM=1.62 mM for cystathionine

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 387387Cystathionine beta-lyase patB
PRO_0000163848

Amino acid modifications

Modified residue2341N6-(pyridoxal phosphate)lysine By similarity

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Q08432-1 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 959A4B1C500D5CFB

FASTA38742,789
        10         20         30         40         50         60 
MNFDKREERL GTQSVKWDKT GELFGVTDAL PMWVADMDFR APEAITEALK ERLDHGIFGY 

        70         80         90        100        110        120 
TTPDQKTKDA VCGWMQNRHG WKVNPESITF SPGVVTALSM AVQAFTEPGD QVVVQPPVYT 

       130        140        150        160        170        180 
PFYHMVEKNG RHILHNPLLE KDGAYAIDFE DLETKLSDPS VTLFILCNPH NPSGRSWSRE 

       190        200        210        220        230        240 
DLLKLGELCL EHGVTVVSDE IHSDLMLYGH KHTPFASLSD DFADISVTCA APSKTFNIAG 

       250        260        270        280        290        300 
LQASAIIIPD RLKRAKFSAS LQRNGLGGLN AFAVTAIEAA YSKGGPWLDE LITYIEKNMN 

       310        320        330        340        350        360 
EAEAFLSTEL PKVKMMKPDA SYLIWLDFSA YGLSDAELQQ RMLKKGKVIL EPGTKYGPGG 

       370        380 
EGFMRLNAGC SLATLQDGLR RIKAALS

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Multisensory activation of the phosphorelay initiating sporulation in Bacillus subtilis: identification and sequence of the protein kinase of the alternate pathway."
Trach K.A., Hoch J.A.
Mol. Microbiol. 8:69-79(1993) [PubMed: 8497199] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai)."
Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H., Duesterhoeft A., Pohl T.M., Weitzenegger T.
Microbiology 143:2769-2774(1997) [PubMed: 9274030] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"The PatB protein of Bacillus subtilis is a C-S-lyase."
Auger S., Gomez M.P., Danchin A., Martin-Verstraete I.
Biochimie 87:231-238(2005) [PubMed: 15760717] [Abstract]
Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION, DISRUPTION PHENOTYPE.
Strain: 168.

Hide | Top

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U63302 Genomic DNA. Translation: AAB61979.1.
Z93933 Genomic DNA. Translation: CAB07910.1.
Z93934 Genomic DNA. Translation: CAB07924.1.
AL009126 Genomic DNA. Translation: CAB15133.1.
PIRS32934.
RefSeqNP_391022.1.

3D structure databases

SMRQ08432. Positions 2-387.
ModBaseSearch...

Genome annotation databases

GeneID937170.
GenomeReviewsGene locus BSU31440 in contig AL009126_GR.
KEGGbsu:BSU31440.
NMPDRfig|224308.1.peg.3147.

Organism-specific databases

SubtiListBG10744. patB. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMHBG681988.
OMAYLMWLDC.
PhylomeDBQ08432.

Family and domain databases

InterProIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase_major_dom.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
Gene3DG3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PfamPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
PROSITEPS00599. AA_TRANSFER_CLASS_2. False negative.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameCBL_BACSU
AccessionPrimary (citable) accession number: Q08432
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: January 19, 2010
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents