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Protein

Lactadherin

Gene

MFGE8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.By similarity1 Publication
Medin is the main constituent of aortic medial amyloid.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Angiogenesis, Cell adhesion, Fertilization, Host-virus interaction

Enzyme and pathway databases

ReactomeiR-HSA-977225. Amyloid fiber formation.

Names & Taxonomyi

Protein namesi
Recommended name:
Lactadherin
Alternative name(s):
Breast epithelial antigen BA46
HMFG
MFGM
Milk fat globule-EGF factor 8
Short name:
MFG-E8
SED1
Cleaved into the following 2 chains:
Gene namesi
Name:MFGE8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:7036. MFGE8.

Subcellular locationi

GO - Cellular componenti

  • external side of plasma membrane Source: Ensembl
  • extracellular exosome Source: UniProtKB
  • extracellular region Source: Reactome
  • extracellular space Source: UniProtKB
  • extracellular vesicle Source: UniProtKB
  • extrinsic component of plasma membrane Source: Ensembl
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Amyloid, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30772.

Polymorphism and mutation databases

BioMutaiMFGE8.
DMDMi2506380.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Add
BLAST
Chaini24 – 387364LactadherinPRO_0000007650Add
BLAST
Chaini202 – 387186Lactadherin short formPRO_0000007651Add
BLAST
Chaini268 – 31750MedinPRO_0000007652Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi27 ↔ 38By similarity
Disulfide bondi32 ↔ 55By similarity
Modified residuei42 – 421Phosphoserine; by FAM20C1 Publication
Disulfide bondi57 ↔ 66By similarity
Disulfide bondi70 ↔ 225By similarity
Disulfide bondi212 ↔ 216By similarity
Glycosylationi228 – 2281N-linked (GlcNAc...); atypical1 Publication
Disulfide bondi230 ↔ 387By similarity
Glycosylationi238 – 2381N-linked (GlcNAc...)1 Publication
Glycosylationi325 – 3251N-linked (GlcNAc...)1 Publication
Glycosylationi329 – 3291N-linked (GlcNAc...)1 Publication
Glycosylationi350 – 3501N-linked (GlcNAc...)1 Publication

Post-translational modificationi

Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ08431.
MaxQBiQ08431.
PaxDbiQ08431.
PeptideAtlasiQ08431.
PRIDEiQ08431.

PTM databases

iPTMnetiQ08431.
PhosphoSiteiQ08431.

Expressioni

Tissue specificityi

Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.

Gene expression databases

BgeeiENSG00000140545.
CleanExiHS_MFGE8.
ExpressionAtlasiQ08431. baseline and differential.
GenevisibleiQ08431. HS.

Organism-specific databases

HPAiCAB002753.
HPA002807.

Interactioni

Protein-protein interaction databases

BioGridi110398. 5 interactions.
IntActiQ08431. 1 interaction.
MINTiMINT-5004478.
STRINGi9606.ENSP00000268150.

Structurei

3D structure databases

ProteinModelPortaliQ08431.
SMRiQ08431. Positions 27-387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 6744EGF-likePROSITE-ProRule annotationAdd
BLAST
Domaini70 – 225156F5/8 type C 1PROSITE-ProRule annotationAdd
BLAST
Domaini230 – 387158F5/8 type C 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 483Cell attachment site

Domaini

The F5/8 type C 2 domain mediates high-affinity binding to phosphatidylserine-containing membranes.By similarity

Sequence similaritiesi

Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 2 F5/8 type C domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IFBC. Eukaryota.
ENOG41114BV. LUCA.
HOGENOMiHOG000236278.
HOVERGENiHBG002385.
InParanoidiQ08431.
KOiK17253.
OrthoDBiEOG091G071G.
PhylomeDBiQ08431.
TreeFamiTF330156.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000421. FA58C.
IPR008979. Galactose-bd-like.
IPR027060. Lactadherin.
[Graphical view]
PANTHERiPTHR10127:SF303. PTHR10127:SF303. 1 hit.
PfamiPF00008. EGF. 1 hit.
PF00754. F5_F8_type_C. 2 hits.
[Graphical view]
SMARTiSM00181. EGF. 1 hit.
SM00231. FA58C. 2 hits.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 2 hits.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS01285. FA58C_1. 2 hits.
PS01286. FA58C_2. 2 hits.
PS50022. FA58C_3. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q08431-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPRPRLLAAL CGALLCAPSL LVALDICSKN PCHNGGLCEE ISQEVRGDVF
60 70 80 90 100
PSYTCTCLKG YAGNHCETKC VEPLGMENGN IANSQIAASS VRVTFLGLQH
110 120 130 140 150
WVPELARLNR AGMVNAWTPS SNDDNPWIQV NLLRRMWVTG VVTQGASRLA
160 170 180 190 200
SHEYLKAFKV AYSLNGHEFD FIHDVNKKHK EFVGNWNKNA VHVNLFETPV
210 220 230 240 250
EAQYVRLYPT SCHTACTLRF ELLGCELNGC ANPLGLKNNS IPDKQITASS
260 270 280 290 300
SYKTWGLHLF SWNPSYARLD KQGNFNAWVA GSYGNDQWLQ VDLGSSKEVT
310 320 330 340 350
GIITQGARNF GSVQFVASYK VAYSNDSANW TEYQDPRTGS SKIFPGNWDN
360 370 380
HSHKKNLFET PILARYVRIL PVAWHNRIAL RLELLGC
Length:387
Mass (Da):43,123
Last modified:November 1, 1997 - v2
Checksum:i2EE6571DEC83782D
GO
Isoform 2 (identifier: Q08431-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-75: Missing.

Note: No experimental confirmation available.
Show »
Length:312
Mass (Da):35,189
Checksum:i9E3857A7E7096C8B
GO
Isoform 3 (identifier: Q08431-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     291-342: Missing.

Show »
Length:335
Mass (Da):37,523
Checksum:i20E84E5DB78E8E25
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti268 – 2681R → W in CAD97938 (Ref. 5) Curated
Sequence conflicti352 – 3521S → T in CAD97938 (Ref. 5) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti3 – 31R → S.
Corresponds to variant rs4945 [ dbSNP | Ensembl ].
VAR_029794
Natural varianti76 – 761M → L.3 Publications
Corresponds to variant rs1878326 [ dbSNP | Ensembl ].
VAR_024263

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7575Missing in isoform 2. 1 PublicationVSP_039108Add
BLAST
Alternative sequencei291 – 34252Missing in isoform 3. 2 PublicationsVSP_039953Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58516 mRNA. Translation: AAC50549.1.
AY141974 mRNA. Translation: AAN08508.1.
BT006948 mRNA. Translation: AAP35594.1.
AK312640 mRNA. Translation: BAG35524.1.
AK223182 mRNA. Translation: BAD96902.1.
AK222735 mRNA. Translation: BAD96455.1.
BX537974 mRNA. Translation: CAD97938.1.
AC067805 Genomic DNA. No translation available.
CH471101 Genomic DNA. Translation: EAX02026.1.
BC003610 mRNA. Translation: AAH03610.1.
S56151 mRNA. Translation: AAB19771.1.
CCDSiCCDS10347.1. [Q08431-1]
CCDS45345.1. [Q08431-3]
PIRiA47285.
RefSeqiNP_001108086.1. NM_001114614.2.
NP_001297248.1. NM_001310319.1.
NP_001297249.1. NM_001310320.1.
NP_001297250.1. NM_001310321.1.
NP_005919.2. NM_005928.3.
UniGeneiHs.3745.

Genome annotation databases

EnsembliENST00000268150; ENSP00000268150; ENSG00000140545.
ENST00000566497; ENSP00000456281; ENSG00000140545.
GeneIDi4240.
KEGGihsa:4240.
UCSCiuc002bng.5. human. [Q08431-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58516 mRNA. Translation: AAC50549.1.
AY141974 mRNA. Translation: AAN08508.1.
BT006948 mRNA. Translation: AAP35594.1.
AK312640 mRNA. Translation: BAG35524.1.
AK223182 mRNA. Translation: BAD96902.1.
AK222735 mRNA. Translation: BAD96455.1.
BX537974 mRNA. Translation: CAD97938.1.
AC067805 Genomic DNA. No translation available.
CH471101 Genomic DNA. Translation: EAX02026.1.
BC003610 mRNA. Translation: AAH03610.1.
S56151 mRNA. Translation: AAB19771.1.
CCDSiCCDS10347.1. [Q08431-1]
CCDS45345.1. [Q08431-3]
PIRiA47285.
RefSeqiNP_001108086.1. NM_001114614.2.
NP_001297248.1. NM_001310319.1.
NP_001297249.1. NM_001310320.1.
NP_001297250.1. NM_001310321.1.
NP_005919.2. NM_005928.3.
UniGeneiHs.3745.

3D structure databases

ProteinModelPortaliQ08431.
SMRiQ08431. Positions 27-387.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110398. 5 interactions.
IntActiQ08431. 1 interaction.
MINTiMINT-5004478.
STRINGi9606.ENSP00000268150.

PTM databases

iPTMnetiQ08431.
PhosphoSiteiQ08431.

Polymorphism and mutation databases

BioMutaiMFGE8.
DMDMi2506380.

Proteomic databases

EPDiQ08431.
MaxQBiQ08431.
PaxDbiQ08431.
PeptideAtlasiQ08431.
PRIDEiQ08431.

Protocols and materials databases

DNASUi4240.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000268150; ENSP00000268150; ENSG00000140545.
ENST00000566497; ENSP00000456281; ENSG00000140545.
GeneIDi4240.
KEGGihsa:4240.
UCSCiuc002bng.5. human. [Q08431-1]

Organism-specific databases

CTDi4240.
GeneCardsiMFGE8.
H-InvDBHIX0012558.
HGNCiHGNC:7036. MFGE8.
HPAiCAB002753.
HPA002807.
MIMi602281. gene.
neXtProtiNX_Q08431.
PharmGKBiPA30772.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFBC. Eukaryota.
ENOG41114BV. LUCA.
HOGENOMiHOG000236278.
HOVERGENiHBG002385.
InParanoidiQ08431.
KOiK17253.
OrthoDBiEOG091G071G.
PhylomeDBiQ08431.
TreeFamiTF330156.

Enzyme and pathway databases

ReactomeiR-HSA-977225. Amyloid fiber formation.

Miscellaneous databases

ChiTaRSiMFGE8. human.
GeneWikiiMFGE8.
GenomeRNAii4240.
PROiQ08431.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000140545.
CleanExiHS_MFGE8.
ExpressionAtlasiQ08431. baseline and differential.
GenevisibleiQ08431. HS.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000421. FA58C.
IPR008979. Galactose-bd-like.
IPR027060. Lactadherin.
[Graphical view]
PANTHERiPTHR10127:SF303. PTHR10127:SF303. 1 hit.
PfamiPF00008. EGF. 1 hit.
PF00754. F5_F8_type_C. 2 hits.
[Graphical view]
SMARTiSM00181. EGF. 1 hit.
SM00231. FA58C. 2 hits.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 2 hits.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS01285. FA58C_1. 2 hits.
PS01286. FA58C_2. 2 hits.
PS50022. FA58C_3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMFGM_HUMAN
AccessioniPrimary (citable) accession number: Q08431
Secondary accession number(s): B2R6M7
, Q53FU9, Q7Z3D2, Q9BTL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.