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Protein

Lactadherin

Gene

MFGE8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.By similarity1 Publication
Medin is the main constituent of aortic medial amyloid.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAngiogenesis, Cell adhesion, Fertilization, Host-virus interaction

Enzyme and pathway databases

ReactomeiR-HSA-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-HSA-8957275 Post-translational protein phosphorylation
R-HSA-977225 Amyloid fiber formation

Names & Taxonomyi

Protein namesi
Recommended name:
Lactadherin
Alternative name(s):
Breast epithelial antigen BA46
HMFG
MFGM
Milk fat globule-EGF factor 8
Short name:
MFG-E8
SED1
Cleaved into the following 2 chains:
Gene namesi
Name:MFGE8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000140545.14
HGNCiHGNC:7036 MFGE8
MIMi602281 gene
neXtProtiNX_Q08431

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Amyloid, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi4240
PharmGKBiPA30772

Polymorphism and mutation databases

BioMutaiMFGE8
DMDMi2506380

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Add BLAST23
ChainiPRO_000000765024 – 387LactadherinAdd BLAST364
ChainiPRO_0000007651202 – 387Lactadherin short formAdd BLAST186
ChainiPRO_0000007652268 – 317MedinAdd BLAST50

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi27 ↔ 38By similarity
Disulfide bondi32 ↔ 55By similarity
Modified residuei42Phosphoserine; by FAM20C1 Publication1
Disulfide bondi57 ↔ 66By similarity
Disulfide bondi70 ↔ 225By similarity
Disulfide bondi212 ↔ 216By similarity
Glycosylationi228N-linked (GlcNAc...) asparagine; atypical1 Publication1
Disulfide bondi230 ↔ 387By similarity
Glycosylationi238N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi325N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi329N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi350N-linked (GlcNAc...) asparagine1 Publication1

Post-translational modificationi

Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ08431
MaxQBiQ08431
PaxDbiQ08431
PeptideAtlasiQ08431
PRIDEiQ08431

PTM databases

iPTMnetiQ08431
PhosphoSitePlusiQ08431

Expressioni

Tissue specificityi

Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.

Gene expression databases

BgeeiENSG00000140545
CleanExiHS_MFGE8
ExpressionAtlasiQ08431 baseline and differential
GenevisibleiQ08431 HS

Organism-specific databases

HPAiCAB002753
HPA002807

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110398, 6 interactors
IntActiQ08431, 2 interactors
STRINGi9606.ENSP00000268150

Structurei

3D structure databases

ProteinModelPortaliQ08431
SMRiQ08431
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 67EGF-likePROSITE-ProRule annotationAdd BLAST44
Domaini70 – 225F5/8 type C 1PROSITE-ProRule annotationAdd BLAST156
Domaini230 – 387F5/8 type C 2PROSITE-ProRule annotationAdd BLAST158

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi46 – 48Cell attachment site3

Domaini

The F5/8 type C 2 domain mediates high-affinity binding to phosphatidylserine-containing membranes.By similarity

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IFBC Eukaryota
ENOG41114BV LUCA
HOGENOMiHOG000236278
HOVERGENiHBG002385
InParanoidiQ08431
KOiK17253
OrthoDBiEOG091G071G
PhylomeDBiQ08431
TreeFamiTF330156

Family and domain databases

Gene3Di2.60.120.260, 2 hits
InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000421 FA58C
IPR008979 Galactose-bd-like_sf
IPR027060 Lactadherin
PANTHERiPTHR44122:SF1 PTHR44122:SF1, 2 hits
PfamiView protein in Pfam
PF00008 EGF, 1 hit
PF00754 F5_F8_type_C, 2 hits
SMARTiView protein in SMART
SM00181 EGF, 1 hit
SM00231 FA58C, 2 hits
SUPFAMiSSF49785 SSF49785, 2 hits
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 1 hit
PS01285 FA58C_1, 2 hits
PS01286 FA58C_2, 2 hits
PS50022 FA58C_3, 2 hits

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q08431-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPRPRLLAAL CGALLCAPSL LVALDICSKN PCHNGGLCEE ISQEVRGDVF
60 70 80 90 100
PSYTCTCLKG YAGNHCETKC VEPLGMENGN IANSQIAASS VRVTFLGLQH
110 120 130 140 150
WVPELARLNR AGMVNAWTPS SNDDNPWIQV NLLRRMWVTG VVTQGASRLA
160 170 180 190 200
SHEYLKAFKV AYSLNGHEFD FIHDVNKKHK EFVGNWNKNA VHVNLFETPV
210 220 230 240 250
EAQYVRLYPT SCHTACTLRF ELLGCELNGC ANPLGLKNNS IPDKQITASS
260 270 280 290 300
SYKTWGLHLF SWNPSYARLD KQGNFNAWVA GSYGNDQWLQ VDLGSSKEVT
310 320 330 340 350
GIITQGARNF GSVQFVASYK VAYSNDSANW TEYQDPRTGS SKIFPGNWDN
360 370 380
HSHKKNLFET PILARYVRIL PVAWHNRIAL RLELLGC
Length:387
Mass (Da):43,123
Last modified:November 1, 1997 - v2
Checksum:i2EE6571DEC83782D
GO
Isoform 2 (identifier: Q08431-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-75: Missing.

Note: No experimental confirmation available.
Show »
Length:312
Mass (Da):35,189
Checksum:i9E3857A7E7096C8B
GO
Isoform 3 (identifier: Q08431-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     291-342: Missing.

Show »
Length:335
Mass (Da):37,523
Checksum:i20E84E5DB78E8E25
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti268R → W in CAD97938 (Ref. 5) Curated1
Sequence conflicti352S → T in CAD97938 (Ref. 5) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0297943R → S. Corresponds to variant dbSNP:rs4945Ensembl.1
Natural variantiVAR_02426376M → L3 PublicationsCorresponds to variant dbSNP:rs1878326Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0391081 – 75Missing in isoform 2. 1 PublicationAdd BLAST75
Alternative sequenceiVSP_039953291 – 342Missing in isoform 3. 2 PublicationsAdd BLAST52

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58516 mRNA Translation: AAC50549.1
AY141974 mRNA Translation: AAN08508.1
BT006948 mRNA Translation: AAP35594.1
AK312640 mRNA Translation: BAG35524.1
AK223182 mRNA Translation: BAD96902.1
AK222735 mRNA Translation: BAD96455.1
BX537974 mRNA Translation: CAD97938.1
AC067805 Genomic DNA No translation available.
CH471101 Genomic DNA Translation: EAX02026.1
BC003610 mRNA Translation: AAH03610.1
S56151 mRNA Translation: AAB19771.1
CCDSiCCDS10347.1 [Q08431-1]
CCDS45345.1 [Q08431-3]
PIRiA47285
RefSeqiNP_001108086.1, NM_001114614.2
NP_001297248.1, NM_001310319.1
NP_001297249.1, NM_001310320.1
NP_001297250.1, NM_001310321.1
NP_005919.2, NM_005928.3
UniGeneiHs.3745
Hs.720851

Genome annotation databases

EnsembliENST00000268150; ENSP00000268150; ENSG00000140545
ENST00000566497; ENSP00000456281; ENSG00000140545
GeneIDi4240
KEGGihsa:4240
UCSCiuc002bng.5 human [Q08431-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiMFGM_HUMAN
AccessioniPrimary (citable) accession number: Q08431
Secondary accession number(s): B2R6M7
, Q53FU9, Q7Z3D2, Q9BTL9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

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