Reviewed, UniProtKB/Swiss-Prot Q08410 (TYRO_COTJA)

Names and origin

Protein names

Recommended name:
    Tyrosinase
    EC=1.14.18.1
Alternative name(s):
    Monophenol monooxygenase

Gene names

Name:

TYR

Organism

Coturnix coturnix japonica (Japanese quail)

Taxonomic identifier

93934 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Perdicinae › Coturnix

Protein attributes

Sequence length	273 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
Catalytic activity	L-tyrosine + L-dopa + O2 = L-dopa + dopaquinone + H2O.
Cofactor	Binds 2 copper ions per subunit.
Subcellular location	Melanosome membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the tyrosinase family.

Ontologies

Keywords
Biological process	Melanin biosynthesis
Cellular component	Membrane
Domain	Signal Transmembrane
Ligand	Copper Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	melanin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW monophenol monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – ›273	›255	Tyrosinase		PRO_0000035882
Sites
	Metal binding	180	1	Copper A By similarity
	Metal binding	202	1	Copper A By similarity
	Metal binding	211	1	Copper A By similarity
Amino acid modifications
	Glycosylation	86	1	N-linked (GlcNAc...) Potential
	Glycosylation	111	1	N-linked (GlcNAc...) Potential
	Glycosylation	161	1	N-linked (GlcNAc...) Potential
	Glycosylation	230	1	N-linked (GlcNAc...) Potential
Experimental info
	Non-terminal residue	273	1

Sequences

	Sequence		Length	Mass (Da)
	Q08410-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 0EA3DE55BE11EA1A	FASTA	273	31,500
	10 20 30 40 50 60 MLLFTMGLLL AILQPSTGQF PRVCANTQSL LRKECCPPWE GDGSPCGERS NRGTCQRILL 70 80 90 100 110 120 SQAPLGPQFP FSGVDDREDW PSVFYNRTCR CRGNFMGFNC GECKFGFSGQ NCTERRLRTR 130 140 150 160 170 180 RNIFQLTIRE KDKFLAYLNL AKNIPSKDYV IATGTYAQMN NGSNPMFRNI NVYDLFVWMH 190 200 210 220 230 240 YYASRDTLLG GSNVWRDIDF AHEAPGFLPW HRAFLLLWER EIQKITGDEN FTIPYWDWRD 250 260 270 AEDCVICTDE YMGGQHPTNP NLLSPASFFS SWQ

References

[1]	"Phylogeny of regulatory regions of vertebrate tyrosinase genes." Yamamoto H., Kudo T., Masuko N., Miura H., Sato S., Tanaka M., Tanaka S., Takeuchi S., Shibahara S., Takeuchi T. Pigment Cell Res. 5:284-294(1992) [PubMed: 1292011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB024279 Genomic DNA. Translation: BAB21535.1. S56788 Genomic DNA. Translation: AAB25510.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q08410.
Enzyme and pathway databases
BRENDA	1.14.18.1. 142881.
Family and domain databases
InterPro	IPR008922. Di-copper_centre. IPR002227. Tyrosinase. [Graphical view]
Gene3D	G3DSA:1.10.1280.10. Di-copper_centre. 1 hit.
Pfam	PF00264. Tyrosinase. 1 hit. [Graphical view]
PRINTS	PR00092. TYROSINASE.
PROSITE	PS00497. TYROSINASE_1. 1 hit. PS00498. TYROSINASE_2. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYRO_COTJA

Accession

Primary (citable) accession number: Q08410
Secondary accession number(s): Q54AH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	October 13, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)