ID   PEMT_RAT                Reviewed;         199 AA.
AC   Q08388; Q5BK89;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   16-JUN-2009, entry version 54.
DE   RecName: Full=Phosphatidylethanolamine N-methyltransferase;
DE            Short=PEAMT;
DE            Short=PEMT;
DE            EC=2.1.1.17;
GN   Name=Pemt; Synonyms=Pempt;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver;
RX   MEDLINE=93346418; PubMed=8344945;
RA   Cui Z., Vance J.E., Chen M.H., Voelker D.R., Vance D.E.;
RT   "Cloning and expression of a novel phosphatidylethanolamine N-
RT   methyltransferase. A specific biochemical and cytological marker for a
RT   unique membrane fraction in rat liver.";
RL   J. Biol. Chem. 268:16655-16663(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Liver;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   PROTEIN SEQUENCE OF 2-31.
RA   Ridgway N.D.;
RL   Thesis (1988), University of British Columbia, Canada.
CC   -!- FUNCTION: Catalyzes three sequential methylation of
CC       phosphatidylethanolamine (PE) by AdoMet, thus producing
CC       phosphatidylcholine (PC).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine +
CC       phosphatidylethanolamine = S-adenosyl-L-homocysteine +
CC       phosphatidyl-N-methylethanolamine.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC       membrane protein (Potential). Mitochondrion membrane; Multi-pass
CC       membrane protein (Potential).
CC   -!- TISSUE SPECIFICITY: Liver.
CC   -!- SIMILARITY: Belongs to the PEMT/PEM2 methyltransferase family.
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DR   EMBL; L14441; AAA03154.1; -; mRNA.
DR   EMBL; BC091162; AAH91162.1; -; mRNA.
DR   IPI; IPI00231668; -.
DR   PIR; A47353; A47353.
DR   RefSeq; NP_037135.1; -.
DR   UniGene; Rn.9875; -.
DR   PRIDE; Q08388; -.
DR   GeneID; 25511; -.
DR   KEGG; rno:25511; -.
DR   RGD; 3297; Pemt.
DR   HOVERGEN; Q08388; -.
DR   BRENDA; 2.1.1.17; 248.
DR   NextBio; 606945; -.
DR   GO; GO:0031526; C:brush border membrane; IDA:RGD.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005624; C:membrane fraction; IDA:RGD.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042383; C:sarcolemma; IDA:RGD.
DR   GO; GO:0008429; F:phosphatidylethanolamine binding; IDA:RGD.
DR   GO; GO:0004608; F:phosphatidylethanolamine N-methyltransferas...; IDA:RGD.
DR   GO; GO:0008285; P:negative regulation of cell proliferation; IMP:RGD.
DR   GO; GO:0006656; P:phosphatidylcholine biosynthetic process; IDA:RGD.
DR   GO; GO:0050747; P:positive regulation of lipoprotein metaboli...; IMP:RGD.
DR   GO; GO:0043200; P:response to amino acid stimulus; IEP:RGD.
DR   GO; GO:0042493; P:response to drug; IDA:RGD.
DR   GO; GO:0045471; P:response to ethanol; IEP:RGD.
DR   GO; GO:0033273; P:response to vitamin; IDA:RGD.
DR   GO; GO:0046498; P:S-adenosylhomocysteine metabolic process; IDA:RGD.
DR   GO; GO:0046500; P:S-adenosylmethionine metabolic process; IDA:RGD.
DR   InterPro; IPR007318; PEMT.
DR   PANTHER; PTHR15458; PEMT; 1.
DR   Pfam; PF04191; PEMT; 1.
DR   PIRSF; PIRSF005444; PEMT; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Endoplasmic reticulum; Membrane;
KW   Methyltransferase; Mitochondrion; Phospholipid biosynthesis;
KW   S-adenosyl-L-methionine; Transferase; Transmembrane.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    199       Phosphatidylethanolamine N-
FT                                methyltransferase.
FT                                /FTId=PRO_0000193922.
FT   TRANSMEM     13     33       Potential.
FT   TRANSMEM     46     66       Potential.
FT   TRANSMEM     91    111       Potential.
FT   TRANSMEM    159    179       Potential.
SQ   SEQUENCE   199 AA;  22486 MW;  8F1A938ED883B0FA CRC64;
     MSWLLGYVDP TEPSFVAAVL TIVFNPLFWN VVARWEQRTR KLSRAFGSPY LACYSLGSII
     LLLNILRSHC FTQAMMSQPK MEGLDSHTIY FLGLALLGWG LVFVLSSFYA LGFTGTFLGD
     YFGILKESRV TTFPFSVLDN PMYWGSTANY LGWALMHASP TGLLLTVLVA LVYVVALLFE
     EPFTAEIYRR KATRLHKRS
//