Q08388 (PEMT_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylethanolamine N-methyltransferase
Short name=PEAMT
Short name=PEMT
EC=2.1.1.17

Gene names

Name:	Pemt
Synonyms:	Pempt

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes three sequential methylation of phosphatidylethanolamine (PE) by AdoMet, thus producing phosphatidylcholine (PC).
Catalytic activity	S-adenosyl-L-methionine + phosphatidylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential. Mitochondrion membrane; Multi-pass membrane protein Potential.
Tissue specificity	Liver.
Sequence similarities	Belongs to the PEMT/PEM2 methyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion
Domain	Transmembrane Transmembrane helix
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	S-adenosylhomocysteine metabolic process Inferred from direct assay Ref.1. Source: RGD S-adenosylmethionine metabolic process Inferred from direct assay Ref.1. Source: RGD negative regulation of cell proliferation Inferred from mutant phenotype. Source: RGD phosphatidylcholine biosynthetic process Inferred from direct assay Ref.1. Source: RGD positive regulation of lipoprotein metabolic process Inferred from mutant phenotype. Source: RGD response to amino acid stimulus Inferred from expression pattern. Source: RGD response to drug Inferred from direct assay. Source: RGD response to ethanol Inferred from expression pattern. Source: RGD response to vitamin Inferred from direct assay. Source: RGD
Cellular component	brush border membrane Inferred from direct assay. Source: RGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane fraction Inferred from direct assay Ref.1. Source: RGD mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell sarcolemma Inferred from direct assay. Source: RGD
Molecular function	phosphatidylethanolamine N-methyltransferase activity Inferred from direct assay Ref.1. Source: RGD phosphatidylethanolamine binding Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 199	198	Phosphatidylethanolamine N-methyltransferase		PRO_0000193922
Regions
	Transmembrane	13 – 33	21	Helical; Potential
	Transmembrane	46 – 66	21	Helical; Potential
	Transmembrane	91 – 111	21	Helical; Potential
	Transmembrane	159 – 179	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q08388 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 8F1A938ED883B0FA	FASTA	199	22,486
	10 20 30 40 50 60 MSWLLGYVDP TEPSFVAAVL TIVFNPLFWN VVARWEQRTR KLSRAFGSPY LACYSLGSII 70 80 90 100 110 120 LLLNILRSHC FTQAMMSQPK MEGLDSHTIY FLGLALLGWG LVFVLSSFYA LGFTGTFLGD 130 140 150 160 170 180 YFGILKESRV TTFPFSVLDN PMYWGSTANY LGWALMHASP TGLLLTVLVA LVYVVALLFE 190 EPFTAEIYRR KATRLHKRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a novel phosphatidylethanolamine N-methyltransferase. A specific biochemical and cytological marker for a unique membrane fraction in rat liver." Cui Z., Vance J.E., Chen M.H., Voelker D.R., Vance D.E. J. Biol. Chem. 268:16655-16663(1993) [PubMed: 8344945] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[3]	Ridgway N.D. Thesis (1988), University of British Columbia, Canada Cited for: PROTEIN SEQUENCE OF 2-31.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L14441 mRNA. Translation: AAA03154.1. BC091162 mRNA. Translation: AAH91162.1.
IPI	IPI00231668.
PIR	A47353.
RefSeq	NP_037135.1. NM_013003.1.
UniGene	Rn.9875.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q08388.
Proteomic databases
PRIDE	Q08388.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25511.
KEGG	rno:25511.
Organism-specific databases
CTD	10400.
RGD	3297. Pemt.
Phylogenomic databases
GeneTree	ENSGT00390000007041.
HOVERGEN	HBG000990.
OrthoDB	EOG4V9TRP.
PhylomeDB	Q08388.
Gene expression databases
Genevestigator	Q08388.
Family and domain databases
InterPro	IPR007318. PEMT. [Graphical view]
KO	K00551.
PANTHER	PTHR15458. PEMT. 1 hit.
Pfam	PF04191. PEMT. 1 hit. [Graphical view]
PIRSF	PIRSF005444. PEMT. 1 hit.
ProtoNet	Search...
Other
NextBio	606945.

Entry information

Entry name

PEMT_RAT

Accession

Primary (citable) accession number: Q08388
Secondary accession number(s): Q5BK89

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program