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Q08380

- LG3BP_HUMAN

UniProt

Q08380 - LG3BP_HUMAN

Protein

Galectin-3-binding protein

Gene

LGALS3BP

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.3 Publications

    GO - Molecular functioni

    1. scavenger receptor activity Source: ProtInc

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW
    2. cellular defense response Source: ProtInc
    3. receptor-mediated endocytosis Source: GOC
    4. signal transduction Source: ProtInc

    Keywords - Biological processi

    Cell adhesion

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Galectin-3-binding protein
    Alternative name(s):
    Basement membrane autoantigen p105
    Lectin galactoside-binding soluble 3-binding protein
    Mac-2-binding protein
    Short name:
    MAC2BP
    Short name:
    Mac-2 BP
    Tumor-associated antigen 90K
    Gene namesi
    Name:LGALS3BP
    Synonyms:M2BP
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:6564. LGALS3BP.

    Subcellular locationi

    GO - Cellular componenti

    1. blood microparticle Source: UniProt
    2. extracellular space Source: UniProt
    3. extracellular vesicular exosome Source: UniProtKB
    4. membrane Source: InterPro
    5. proteinaceous extracellular matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30341.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 18183 PublicationsAdd
    BLAST
    Chaini19 – 585567Galectin-3-binding proteinPRO_0000033230Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi49 ↔ 113
    Disulfide bondi62 ↔ 123
    Glycosylationi69 – 691N-linked (GlcNAc...) (complex)6 Publications
    Disulfide bondi93 ↔ 103
    Glycosylationi125 – 1251N-linked (GlcNAc...)2 Publications
    Glycosylationi192 – 1921N-linked (GlcNAc...)2 Publications
    Glycosylationi362 – 3621N-linked (GlcNAc...)
    Glycosylationi398 – 3981N-linked (GlcNAc...) (complex)4 Publications
    Glycosylationi551 – 5511N-linked (GlcNAc...) (complex)5 Publications
    Glycosylationi580 – 5801N-linked (GlcNAc...) (complex)3 Publications

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiQ08380.
    PaxDbiQ08380.
    PeptideAtlasiQ08380.
    PRIDEiQ08380.

    PTM databases

    PhosphoSiteiQ08380.

    Miscellaneous databases

    PMAP-CutDBQ08380.

    Expressioni

    Tissue specificityi

    Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts.3 Publications

    Gene expression databases

    ArrayExpressiQ08380.
    BgeeiQ08380.
    CleanExiHS_LGALS3BP.
    GenevestigatoriQ08380.

    Organism-specific databases

    HPAiCAB002158.
    HPA000554.

    Interactioni

    Subunit structurei

    Homodimers and homomultimers. The multimers form ring-like structures with a diameter of 30-40 nm. Binds LGALS1 and LGALS3. Binds ITGB1, COL4A1, COL5A1, COL6A1, FN1 and NID.1 Publication

    Protein-protein interaction databases

    BioGridi110150. 39 interactions.
    IntActiQ08380. 27 interactions.
    MINTiMINT-1153112.
    STRINGi9606.ENSP00000262776.

    Structurei

    Secondary structure

    1
    585
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi24 – 285
    Beta strandi34 – 418
    Beta strandi44 – 496
    Helixi55 – 6410
    Beta strandi70 – 745
    Turni76 – 794
    Beta strandi86 – 883
    Helixi100 – 1023
    Helixi115 – 1173
    Beta strandi120 – 1234

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BY2X-ray2.00A19-133[»]
    ProteinModelPortaliQ08380.
    SMRiQ08380. Positions 18-127.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ08380.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 124101SRCRPROSITE-ProRule annotationAdd
    BLAST
    Domaini153 – 22169BTBPROSITE-ProRule annotationAdd
    BLAST
    Domaini260 – 360101BACKAdd
    BLAST

    Sequence similaritiesi

    Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
    Contains 1 SRCR domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG41083.
    HOGENOMiHOG000113318.
    HOVERGENiHBG052323.
    InParanoidiQ08380.
    KOiK17300.
    OMAiQHPSFLF.
    OrthoDBiEOG7JDQXP.
    PhylomeDBiQ08380.
    TreeFamiTF331368.

    Family and domain databases

    Gene3Di3.10.250.10. 1 hit.
    3.30.710.10. 1 hit.
    InterProiIPR011705. BACK.
    IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR001190. SRCR.
    IPR017448. SRCR-like_dom.
    [Graphical view]
    PfamiPF07707. BACK. 1 hit.
    PF00530. SRCR. 1 hit.
    [Graphical view]
    PRINTSiPR00258. SPERACTRCPTR.
    SMARTiSM00875. BACK. 1 hit.
    SM00202. SR. 1 hit.
    [Graphical view]
    SUPFAMiSSF56487. SSF56487. 1 hit.
    PROSITEiPS50097. BTB. 1 hit.
    PS00420. SRCR_1. 1 hit.
    PS50287. SRCR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q08380-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTPPRLFWVW LLVAGTQGVN DGDMRLADGG ATNQGRVEIF YRGQWGTVCD    50
    NLWDLTDASV VCRALGFENA TQALGRAAFG QGSGPIMLDE VQCTGTEASL 100
    ADCKSLGWLK SNCRHERDAG VVCTNETRST HTLDLSRELS EALGQIFDSQ 150
    RGCDLSISVN VQGEDALGFC GHTVILTANL EAQALWKEPG SNVTMSVDAE 200
    CVPMVRDLLR YFYSRRIDIT LSSVKCFHKL ASAYGARQLQ GYCASLFAIL 250
    LPQDPSFQMP LDLYAYAVAT GDALLEKLCL QFLAWNFEAL TQAEAWPSVP 300
    TDLLQLLLPR SDLAVPSELA LLKAVDTWSW GERASHEEVE GLVEKIRFPM 350
    MLPEELFELQ FNLSLYWSHE ALFQKKTLQA LEFHTVPFQL LARYKGLNLT 400
    EDTYKPRIYT SPTWSAFVTD SSWSARKSQL VYQSRRGPLV KYSSDYFQAP 450
    SDYRYYPYQS FQTPQHPSFL FQDKRVSWSL VYLPTIQSCW NYGFSCSSDE 500
    LPVLGLTKSG GSDRTIAYEN KALMLCEGLF VADVTDFEGW KAAIPSALDT 550
    NSSKSTSSFP CPAGHFNGFR TVIRPFYLTN SSGVD 585
    Length:585
    Mass (Da):65,331
    Last modified:November 1, 1996 - v1
    Checksum:iC488C2E99D77435B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti19 – 191V → S AA sequence (PubMed:8757764)Curated
    Sequence conflicti25 – 251R → C AA sequence (PubMed:8454062)Curated
    Sequence conflicti25 – 251R → P AA sequence (PubMed:8454062)Curated
    Sequence conflicti26 – 261L → M AA sequence (PubMed:8757764)Curated
    Sequence conflicti30 – 312GA → ED AA sequence (PubMed:8757764)Curated
    Sequence conflicti36 – 361R → H AA sequence (PubMed:8757764)Curated
    Sequence conflicti36 – 361R → L AA sequence (PubMed:8454062)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L13210 mRNA. Translation: AAA36193.1.
    X79089 mRNA. Translation: CAA55699.1.
    BC002403 mRNA. Translation: AAH02403.1.
    BC002998 mRNA. Translation: AAH02998.1.
    BC015761 mRNA. Translation: AAH15761.1.
    CCDSiCCDS11759.1.
    PIRiA47161.
    A55899.
    RefSeqiNP_005558.1. NM_005567.3.
    UniGeneiHs.514535.

    Genome annotation databases

    EnsembliENST00000262776; ENSP00000262776; ENSG00000108679.
    GeneIDi3959.
    KEGGihsa:3959.
    UCSCiuc002jwh.3. human.

    Polymorphism databases

    DMDMi47115668.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L13210 mRNA. Translation: AAA36193.1 .
    X79089 mRNA. Translation: CAA55699.1 .
    BC002403 mRNA. Translation: AAH02403.1 .
    BC002998 mRNA. Translation: AAH02998.1 .
    BC015761 mRNA. Translation: AAH15761.1 .
    CCDSi CCDS11759.1.
    PIRi A47161.
    A55899.
    RefSeqi NP_005558.1. NM_005567.3.
    UniGenei Hs.514535.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BY2 X-ray 2.00 A 19-133 [» ]
    ProteinModelPortali Q08380.
    SMRi Q08380. Positions 18-127.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110150. 39 interactions.
    IntActi Q08380. 27 interactions.
    MINTi MINT-1153112.
    STRINGi 9606.ENSP00000262776.

    PTM databases

    PhosphoSitei Q08380.

    Polymorphism databases

    DMDMi 47115668.

    Proteomic databases

    MaxQBi Q08380.
    PaxDbi Q08380.
    PeptideAtlasi Q08380.
    PRIDEi Q08380.

    Protocols and materials databases

    DNASUi 3959.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000262776 ; ENSP00000262776 ; ENSG00000108679 .
    GeneIDi 3959.
    KEGGi hsa:3959.
    UCSCi uc002jwh.3. human.

    Organism-specific databases

    CTDi 3959.
    GeneCardsi GC17M076970.
    HGNCi HGNC:6564. LGALS3BP.
    HPAi CAB002158.
    HPA000554.
    MIMi 600626. gene.
    neXtProti NX_Q08380.
    PharmGKBi PA30341.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG41083.
    HOGENOMi HOG000113318.
    HOVERGENi HBG052323.
    InParanoidi Q08380.
    KOi K17300.
    OMAi QHPSFLF.
    OrthoDBi EOG7JDQXP.
    PhylomeDBi Q08380.
    TreeFami TF331368.

    Miscellaneous databases

    EvolutionaryTracei Q08380.
    GeneWikii LGALS3BP.
    GenomeRNAii 3959.
    NextBioi 15536.
    PMAP-CutDB Q08380.
    PROi Q08380.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q08380.
    Bgeei Q08380.
    CleanExi HS_LGALS3BP.
    Genevestigatori Q08380.

    Family and domain databases

    Gene3Di 3.10.250.10. 1 hit.
    3.30.710.10. 1 hit.
    InterProi IPR011705. BACK.
    IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR001190. SRCR.
    IPR017448. SRCR-like_dom.
    [Graphical view ]
    Pfami PF07707. BACK. 1 hit.
    PF00530. SRCR. 1 hit.
    [Graphical view ]
    PRINTSi PR00258. SPERACTRCPTR.
    SMARTi SM00875. BACK. 1 hit.
    SM00202. SR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56487. SSF56487. 1 hit.
    PROSITEi PS50097. BTB. 1 hit.
    PS00420. SRCR_1. 1 hit.
    PS50287. SRCR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a human Mac-2-binding protein, a new member of the superfamily defined by the macrophage scavenger receptor cysteine-rich domain."
      Koths K., Taylor E., Halenbeck R., Casipit C., Wang A.
      J. Biol. Chem. 268:14245-14249(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-34; 188-191; 193-200; 202-207; 230-242; 244-259; 261-267; 324-328 AND 437-440, INTERACTION WITH LGALS3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    2. "The secreted tumor-associated antigen 90K is a potent immune stimulator."
      Ullrich A., Sures I., D'Egido M., Jallal B., Powell T.J., Herbst R., Dreps A., Azam M., Rubinstein M., Natoli C.
      J. Biol. Chem. 269:18401-18407(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, GLYCOSYLATION, TISSUE SPECIFICITY.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Colon and Lung.
    4. "Purification and characterization of a 90 kDa protein released from human tumors and tumor cell lines."
      Iacobelli S., Bucci I., D'Egidio M., Giuliani C., Natoli C., Tinari N., Rubistein M., Schlessinger J.
      FEBS Lett. 319:59-65(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 19-40.
      Tissue: Ascites and Serum.
    5. "The 105-kDa basement membrane autoantigen p105 is N-terminally homologous to a tumor-associated antigen."
      Chan L.S., Woodley D.T.
      J. Invest. Dermatol. 107:209-214(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 19-38, TISSUE SPECIFICITY.
      Tissue: Fibroblast.
    6. "Functional studies on recombinant domains of Mac-2-binding protein."
      Hellstern S., Sasaki T., Fauser C., Lustig A., Timpl R., Engel J.
      J. Biol. Chem. 277:15690-15696(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 442-446, GLYCOSYLATION, INTERACTION WITH LGALS3, SUBUNIT STRUCTURE.
    7. "Glycoprotein 90K/MAC-2BP interacts with galectin-1 and mediates galectin-1-induced cell aggregation."
      Tinari N., Kuwabara I., Huflejt M.E., Shen P.F., Iacobelli S., Liu F.-T.
      Int. J. Cancer 91:167-172(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH LGALS1 AND LGALS3.
    8. Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-69.
      Tissue: Bile.
    9. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
      Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
      J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-69; ASN-125; ASN-192; ASN-398; ASN-551 AND ASN-580.
      Tissue: Plasma.
    10. "Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."
      Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A.
      J. Proteome Res. 5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-69; ASN-398; ASN-551 AND ASN-580.
      Tissue: Saliva.
    11. "Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry."
      Picariello G., Ferranti P., Mamone G., Roepstorff P., Addeo F.
      Proteomics 8:3833-3847(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-69 AND ASN-551.
      Tissue: Milk.
    12. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
      Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
      J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-69; ASN-125; ASN-192; ASN-398 AND ASN-551.
      Tissue: Liver.
    13. Cited for: GLYCOSYLATION AT ASN-69; ASN-398; ASN-551 AND ASN-580.
    14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. "Mac-2 binding protein is a cell-adhesive protein of the extracellular matrix which self-assembles into ring-like structures and binds beta1 integrins, collagens and fibronectin."
      Sasaki T., Brakebusch C., Engel J., Timpl R.
      EMBO J. 17:1606-1613(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 18-133, FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, INTERACTION WITH ITGB1; COL4A1; COL5A1; COL6A1; LGALS3; FN1 AND NID.

    Entry informationi

    Entry nameiLG3BP_HUMAN
    AccessioniPrimary (citable) accession number: Q08380
    Secondary accession number(s): Q7M4S0
    , Q9UCH8, Q9UCH9, Q9UCI0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 134 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3