Q08380 (LG3BP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galectin-3-binding protein

Alternative name(s):
Basement membrane autoantigen p105
Lectin galactoside-binding soluble 3-binding protein
Mac-2-binding protein
Short name=MAC2BP
Short name=Mac-2 BP
Tumor-associated antigen 90K

Gene names

Name:	LGALS3BP
Synonyms:	M2BP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells. Ref.2 Ref.7 Ref.14
Subunit structure	Homodimers and homomultimers. The multimers form ring-like structures with a diameter of 30-40 nm. Binds LGALS1 and LGALS3. Binds ITGB1, COL4A1, COL5A1, COL6A1, FN1 and NID. Ref.6
Subcellular location	Secreted. Secreted › extracellular space › extracellular matrix Ref.1 Ref.14.
Tissue specificity	Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts. Ref.1 Ref.2 Ref.5
Sequence similarities	Contains 1 BACK (BTB/Kelch associated) domain. Contains 1 BTB (POZ) domain. Contains 1 SRCR domain.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Extracellular matrix Secreted
Domain	Signal
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW cellular defense response Traceable author statement Ref.2. Source: ProtInc signal transduction Traceable author statement Ref.1. Source: ProtInc
Cellular_component	extracellular space Traceable author statement Ref.1. Source: ProtInc extracellular vesicular exosome Inferred from direct assay PubMed 21362503. Source: UniProtKB membrane Inferred from electronic annotation. Source: InterPro proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	scavenger receptor activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

18

Ref.1 Ref.4 Ref.5

Chain

19 – 585

567

Galectin-3-binding protein

PRO_0000033230

Regions

Domain

24 – 124

101

SRCR

Domain

153 – 221

69

BTB

Domain

260 – 360

101

BACK

Amino acid modifications

Glycosylation

69

1

N-linked (GlcNAc...) Ref.8 Ref.9 Ref.10 Ref.11 Ref.12

Glycosylation

125

1

N-linked (GlcNAc...) Ref.9 Ref.12

Glycosylation

192

1

N-linked (GlcNAc...) Ref.9 Ref.12

Glycosylation

362

1

N-linked (GlcNAc...)

Glycosylation

398

1

N-linked (GlcNAc...) Ref.9 Ref.10 Ref.12

Glycosylation

551

1

N-linked (GlcNAc...) Ref.9 Ref.10 Ref.11 Ref.12

Glycosylation

580

1

N-linked (GlcNAc...) Ref.9 Ref.10

Disulfide bond

49 ↔ 113

Disulfide bond

62 ↔ 123

Disulfide bond

93 ↔ 103

Experimental info

Sequence conflict

19

1

V → S AA sequence Ref.5

Sequence conflict

25

1

R → C AA sequence Ref.4

Sequence conflict

25

1

R → P AA sequence Ref.4

Sequence conflict

26

1

L → M AA sequence Ref.5

Sequence conflict

30 – 31

2

GA → ED AA sequence Ref.5

Sequence conflict

36

1

R → H AA sequence Ref.5

Sequence conflict

36

1

R → L AA sequence Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

585

Helix Strand Turn

Details...

Beta strand

24 – 28

5

Beta strand

34 – 41

8

Beta strand

44 – 49

6

Helix

55 – 64

10

Beta strand

70 – 74

5

Turn

76 – 79

4

Beta strand

86 – 88

3

Helix

100 – 102

3

Helix

115 – 117

3

Beta strand

120 – 123

4

Sequences

	Sequence		Length	Mass (Da)
	Q08380 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C488C2E99D77435B	FASTA	585	65,331
	10 20 30 40 50 60 MTPPRLFWVW LLVAGTQGVN DGDMRLADGG ATNQGRVEIF YRGQWGTVCD NLWDLTDASV 70 80 90 100 110 120 VCRALGFENA TQALGRAAFG QGSGPIMLDE VQCTGTEASL ADCKSLGWLK SNCRHERDAG 130 140 150 160 170 180 VVCTNETRST HTLDLSRELS EALGQIFDSQ RGCDLSISVN VQGEDALGFC GHTVILTANL 190 200 210 220 230 240 EAQALWKEPG SNVTMSVDAE CVPMVRDLLR YFYSRRIDIT LSSVKCFHKL ASAYGARQLQ 250 260 270 280 290 300 GYCASLFAIL LPQDPSFQMP LDLYAYAVAT GDALLEKLCL QFLAWNFEAL TQAEAWPSVP 310 320 330 340 350 360 TDLLQLLLPR SDLAVPSELA LLKAVDTWSW GERASHEEVE GLVEKIRFPM MLPEELFELQ 370 380 390 400 410 420 FNLSLYWSHE ALFQKKTLQA LEFHTVPFQL LARYKGLNLT EDTYKPRIYT SPTWSAFVTD 430 440 450 460 470 480 SSWSARKSQL VYQSRRGPLV KYSSDYFQAP SDYRYYPYQS FQTPQHPSFL FQDKRVSWSL 490 500 510 520 530 540 VYLPTIQSCW NYGFSCSSDE LPVLGLTKSG GSDRTIAYEN KALMLCEGLF VADVTDFEGW 550 560 570 580 KAAIPSALDT NSSKSTSSFP CPAGHFNGFR TVIRPFYLTN SSGVD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a human Mac-2-binding protein, a new member of the superfamily defined by the macrophage scavenger receptor cysteine-rich domain." Koths K., Taylor E., Halenbeck R., Casipit C., Wang A. J. Biol. Chem. 268:14245-14249(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-34; 188-191; 193-200; 202-207; 230-242; 244-259; 261-267; 324-328 AND 437-440, INTERACTION WITH LGALS3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"The secreted tumor-associated antigen 90K is a potent immune stimulator." Ullrich A., Sures I., D'Egido M., Jallal B., Powell T.J., Herbst R., Dreps A., Azam M., Rubinstein M., Natoli C. J. Biol. Chem. 269:18401-18407(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, GLYCOSYLATION, TISSUE SPECIFICITY.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon and Lung.
[4]	"Purification and characterization of a 90 kDa protein released from human tumors and tumor cell lines." Iacobelli S., Bucci I., D'Egidio M., Giuliani C., Natoli C., Tinari N., Rubistein M., Schlessinger J. FEBS Lett. 319:59-65(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-40. Tissue: Ascites and Serum.
[5]	"The 105-kDa basement membrane autoantigen p105 is N-terminally homologous to a tumor-associated antigen." Chan L.S., Woodley D.T. J. Invest. Dermatol. 107:209-214(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-38, TISSUE SPECIFICITY. Tissue: Fibroblast.
[6]	"Functional studies on recombinant domains of Mac-2-binding protein." Hellstern S., Sasaki T., Fauser C., Lustig A., Timpl R., Engel J. J. Biol. Chem. 277:15690-15696(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 442-446, GLYCOSYLATION, INTERACTION WITH LGALS3, SUBUNIT STRUCTURE.
[7]	"Glycoprotein 90K/MAC-2BP interacts with galectin-1 and mediates galectin-1-induced cell aggregation." Tinari N., Kuwabara I., Huflejt M.E., Shen P.F., Iacobelli S., Liu F.-T. Int. J. Cancer 91:167-172(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH LGALS1 AND LGALS3.
[8]	"A proteomic analysis of human bile." Kristiansen T.Z., Bunkenborg J., Gronborg M., Molina H., Thuluvath P.J., Argani P., Goggins M.G., Maitra A., Pandey A. Mol. Cell. Proteomics 3:715-728(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-69, MASS SPECTROMETRY. Tissue: Bile.
[9]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-69; ASN-125; ASN-192; ASN-398; ASN-551 AND ASN-580, MASS SPECTROMETRY. Tissue: Plasma.
[10]	"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry." Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A. J. Proteome Res. 5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-69; ASN-398; ASN-551 AND ASN-580, MASS SPECTROMETRY. Tissue: Saliva.
[11]	"Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry." Picariello G., Ferranti P., Mamone G., Roepstorff P., Addeo F. Proteomics 8:3833-3847(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-69 AND ASN-551, MASS SPECTROMETRY. Tissue: Milk.
[12]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-69; ASN-125; ASN-192; ASN-398 AND ASN-551, MASS SPECTROMETRY. Tissue: Liver.
[13]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]	"Mac-2 binding protein is a cell-adhesive protein of the extracellular matrix which self-assembles into ring-like structures and binds beta1 integrins, collagens and fibronectin." Sasaki T., Brakebusch C., Engel J., Timpl R. EMBO J. 17:1606-1613(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 18-133, FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, INTERACTION WITH ITGB1; COL4A1; COL5A1; COL6A1; LGALS3; FN1 AND NID.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L13210 mRNA. Translation: AAA36193.1.
X79089 mRNA. Translation: CAA55699.1.
BC002403 mRNA. Translation: AAH02403.1.
BC002998 mRNA. Translation: AAH02998.1.
BC015761 mRNA. Translation: AAH15761.1.

IPI

IPI00023673.

PIR

A47161.
A55899.

RefSeq

NP_005558.1. NM_005567.3.

UniGene

Hs.514535.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BY2	X-ray	2.00	A	19-133	[»]

ProteinModelPortal

Q08380.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q08380. 27 interactions.

MINT

MINT-1153112.

STRING

9606.ENSP00000262776.

PTM databases

PhosphoSite

Q08380.

Polymorphism databases

DMDM

47115668.

Proteomic databases

PaxDb

Q08380.

PeptideAtlas

Q08380.

PRIDE

Q08380.

Protocols and materials databases

DNASU

3959.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000262776; ENSP00000262776; ENSG00000108679.

GeneID

3959.

KEGG

hsa:3959.

UCSC

uc002jwh.3. human.

Organism-specific databases

CTD

3959.

GeneCards

GC17M076970.

HGNC

HGNC:6564. LGALS3BP.

HPA

CAB002158.
HPA000554.

MIM

600626. gene.

neXtProt

NX_Q08380.

PharmGKB

PA30341.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG41083.

HOGENOM

HOG000113318.

HOVERGEN

HBG052323.

InParanoid

Q08380.

OMA

QHPSFLF.

OrthoDB

EOG4B8JCN.

PhylomeDB

Q08380.

Gene expression databases

ArrayExpress

Q08380.

Bgee

Q08380.

CleanEx

HS_LGALS3BP.

Genevestigator

Q08380.

GermOnline

ENSG00000108679. Homo sapiens.

Family and domain databases

Gene3D

3.30.710.10. 1 hit.

InterPro

IPR011705. BACK.
IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR001190. Srcr_rcpt.
IPR017448. Srcr_rcpt-rel.
[Graphical view]

Pfam

PF07707. BACK. 1 hit.
PF00530. SRCR. 1 hit.
[Graphical view]

PRINTS

PR00258. SPERACTRCPTR.

SMART

SM00875. BACK. 1 hit.
SM00202. SR. 1 hit.
[Graphical view]

SUPFAM

SSF54695. BTB/POZ_fold. 1 hit.
SSF56487. Srcr_receptor. 1 hit.

PROSITE

PS50097. BTB. 1 hit.
PS00420. SRCR_1. 1 hit.
PS50287. SRCR_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q08380.

GenomeRNAi

3959.

NextBio

15536.

PMAP-CutDB

Q08380.

SOURCE

Search...

Entry information

Entry name

LG3BP_HUMAN

Accession

Primary (citable) accession number: Q08380
Secondary accession number(s): Q7M4S0 , Q9UCH8, Q9UCH9, Q9UCI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.