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Protein

Golgin subfamily A member 2

Gene

GOLGA2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.

GO - Molecular functioni

  1. protein kinase binding Source: UniProtKB
  2. syntaxin binding Source: UniProtKB

GO - Biological processi

  1. mitotic cell cycle Source: Reactome
  2. negative regulation of protein binding Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_1100. Golgi Cisternae Pericentriolar Stack Reorganization.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgin subfamily A member 2
Alternative name(s):
130 kDa cis-Golgi matrix protein
Short name:
GM130
GM130 autoantigen
Golgin-95
Gene namesi
Name:GOLGA2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:4425. GOLGA2.

Subcellular locationi

GO - Cellular componenti

  1. cis-Golgi network Source: MGI
  2. ER to Golgi transport vesicle Source: UniProtKB
  3. Golgi apparatus Source: UniProtKB
  4. Golgi cisterna membrane Source: UniProtKB-SubCell
  5. Golgi membrane Source: Reactome
  6. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28805.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10021002Golgin subfamily A member 2PRO_0000190054Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei37 – 371Phosphoserine3 Publications
Modified residuei273 – 2731Phosphoserine1 Publication
Modified residuei438 – 4381Phosphoserine1 Publication
Modified residuei690 – 6901Phosphoserine1 Publication
Modified residuei937 – 9371Phosphoserine1 Publication
Modified residuei953 – 9531Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08379.
PaxDbiQ08379.
PRIDEiQ08379.

PTM databases

PhosphoSiteiQ08379.

Expressioni

Gene expression databases

BgeeiQ08379.
CleanExiHS_GOLGA2.
ExpressionAtlasiQ08379. baseline and differential.
GenevestigatoriQ08379.

Organism-specific databases

HPAiCAB015421.
HPA021178.
HPA021230.
HPA021799.

Interactioni

Subunit structurei

Part of a larger oligomeric complex. Interacts with p115 (By similarity). Interacts with RAB1B that has been activated by GTP-binding. Interacts with GORASP1/GRASP65 and ZFPL1.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P299913EBI-618309,EBI-8826488From a different organism.
Q9WMX22EBI-618309,EBI-6863748From a different organism.
AKAP9Q999963EBI-618309,EBI-1048311
STK25O005063EBI-618309,EBI-618295
ZDHHC17Q8IUH53EBI-618309,EBI-524753

Protein-protein interaction databases

BioGridi109063. 297 interactions.
DIPiDIP-34271N.
IntActiQ08379. 61 interactions.
MINTiMINT-1343711.
STRINGi9606.ENSP00000416097.

Structurei

3D structure databases

ProteinModelPortaliQ08379.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili16 – 892877Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi46 – 516Poly-Lys
Compositional biasi449 – 4557Poly-Pro
Compositional biasi704 – 71512Poly-GluAdd
BLAST

Domaini

Extended rod-like protein with coiled-coil domains.

Sequence similaritiesi

Belongs to the GOLGA2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG304043.
GeneTreeiENSGT00530000062932.
HOGENOMiHOG000218631.
HOVERGENiHBG051752.
InParanoidiQ08379.
OMAiDNESLSH.
OrthoDBiEOG71P29T.
PhylomeDBiQ08379.
TreeFamiTF316990.

Family and domain databases

InterProiIPR024858. Golgin_A.
[Graphical view]
PANTHERiPTHR10881. PTHR10881. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q08379-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWPQPRLPPR PAMSEETRQS KLAAAKKKLR EYQQRNSPGV PTGAKKKKKI
60 70 80 90 100
KNGSNPETTT SGGCHSPEDT PKDNAATLQP SDDTVLPGGV PSPGASLTSM
110 120 130 140 150
AASQNHDADN VPNLMDETKT FSSTESLRQL SQQLNGLVCE SATCVNGEGP
160 170 180 190 200
ASSANLKDLE SRYQQLAVAL DSSYVTNKQL NITIEKLKQQ NQEITDQLEE
210 220 230 240 250
EKKECHQKQG ALREQLQVHI QTIGILVSEK AELQTALAHT QHAARQKEGE
260 270 280 290 300
SEDLASRLQY SRRRVGELER ALSAVSTQQK KADRYNKELT KERDALRLEL
310 320 330 340 350
YKNTQSNEDL KQEKSELEEK LRVLVTEKAG MQLNLEELQK KLEMTELLLQ
360 370 380 390 400
QFSSRCEAPD ANQQLQQAME ERAQLEAHLG QVMESVRQLQ MERDKYAENL
410 420 430 440 450
KGESAMWRQR MQQMSEQVHT LREEKECSMS RVQELETSLA ELRNQMAEPP
460 470 480 490 500
PPEPPAGPSE VEQQLQAEAE HLRKELEGLA GQLQAQVQDN EGLSRLNREQ
510 520 530 540 550
EERLLELERA AELWGEQAEA RRQILETMQN DRTTISRALS QNRELKEQLA
560 570 580 590 600
ELQSGFVKLT NENMEITSAL QSEQHVKREL GKKLGELQEK LSELKETVEL
610 620 630 640 650
KSQEAQSLQQ QRDQYLGHLQ QYVAAYQQLT SEKEVLHNQL LLQTQLVDQL
660 670 680 690 700
QQQEAQGKAV AEMARQELQE TQERLEAATQ QNQQLRAQLS LMAHPGEGDG
710 720 730 740 750
LDREEEEDEE EEEEEAVAVP QPMPSIPEDL ESREAMVAFF NSAVASAEEE
760 770 780 790 800
QARLRGQLKE QRVRCRRLAH LLASAQKEPE AAAPAPGTGG DSVCGETHRA
810 820 830 840 850
LQGAMEKLQS RFMELMQEKA DLKERVEELE HRCIQLSGET DTIGEYIALY
860 870 880 890 900
QSQRAVLKER HREKEEYISR LAQDKEEMKV KLLELQELVL RLVGDRNEWH
910 920 930 940 950
GRFLAAAQNP ADEPTSGAPA PQELGAANQQ GDLCEVSLAG SVEPAQGEAR
960 970 980 990 1000
EGSPRDNPTA QQIMQLLREM QNPRERPGLG SNPCIPFFYR ADENDEVKIT

VI
Length:1,002
Mass (Da):113,086
Last modified:April 20, 2010 - v3
Checksum:iBD067145C5092237
GO
Isoform 2 (identifier: Q08379-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-382: Missing.

Show »
Length:620
Mass (Da):70,473
Checksum:i54B31A0FBB42BFC8
GO

Sequence cautioni

The sequence AAH69268.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAP35912.1 differs from that shown.Sequence differs from that shown after position 826 due to an internal deletion.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti709 – 7091Missing in AAP35912 (Ref. 5) Curated
Sequence conflicti762 – 7621R → S in AAP35912 (Ref. 5) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti902 – 9021R → G.
Corresponds to variant rs2240961 [ dbSNP | Ensembl ].
VAR_033974

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 382382Missing in isoform 2. 1 PublicationVSP_007727Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06147 mRNA. Translation: AAA35920.1.
AL590708 Genomic DNA. Translation: CAI13841.2.
BC014188 mRNA. Translation: AAH14188.2.
BC069268 mRNA. Translation: AAH69268.1. Different initiation.
AF248953 mRNA. Translation: AAF65550.1.
BT007248 mRNA. Translation: AAP35912.1. Sequence problems.
CCDSiCCDS6896.2. [Q08379-1]
PIRiJH0821.
RefSeqiNP_004477.3. NM_004486.4. [Q08379-1]
UniGeneiHs.155827.

Genome annotation databases

EnsembliENST00000421699; ENSP00000416097; ENSG00000167110. [Q08379-1]
ENST00000611957; ENSP00000478799; ENSG00000167110. [Q08379-1]
GeneIDi2801.
KEGGihsa:2801.
UCSCiuc011maw.2. human. [Q08379-1]

Polymorphism databases

DMDMi294862511.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06147 mRNA. Translation: AAA35920.1.
AL590708 Genomic DNA. Translation: CAI13841.2.
BC014188 mRNA. Translation: AAH14188.2.
BC069268 mRNA. Translation: AAH69268.1. Different initiation.
AF248953 mRNA. Translation: AAF65550.1.
BT007248 mRNA. Translation: AAP35912.1. Sequence problems.
CCDSiCCDS6896.2. [Q08379-1]
PIRiJH0821.
RefSeqiNP_004477.3. NM_004486.4. [Q08379-1]
UniGeneiHs.155827.

3D structure databases

ProteinModelPortaliQ08379.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109063. 297 interactions.
DIPiDIP-34271N.
IntActiQ08379. 61 interactions.
MINTiMINT-1343711.
STRINGi9606.ENSP00000416097.

PTM databases

PhosphoSiteiQ08379.

Polymorphism databases

DMDMi294862511.

Proteomic databases

MaxQBiQ08379.
PaxDbiQ08379.
PRIDEiQ08379.

Protocols and materials databases

DNASUi2801.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000421699; ENSP00000416097; ENSG00000167110. [Q08379-1]
ENST00000611957; ENSP00000478799; ENSG00000167110. [Q08379-1]
GeneIDi2801.
KEGGihsa:2801.
UCSCiuc011maw.2. human. [Q08379-1]

Organism-specific databases

CTDi2801.
GeneCardsiGC09M131018.
H-InvDBHIX0010915.
HIX0017404.
HIX0177625.
HIX0177626.
HGNCiHGNC:4425. GOLGA2.
HPAiCAB015421.
HPA021178.
HPA021230.
HPA021799.
MIMi602580. gene.
neXtProtiNX_Q08379.
PharmGKBiPA28805.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG304043.
GeneTreeiENSGT00530000062932.
HOGENOMiHOG000218631.
HOVERGENiHBG051752.
InParanoidiQ08379.
OMAiDNESLSH.
OrthoDBiEOG71P29T.
PhylomeDBiQ08379.
TreeFamiTF316990.

Enzyme and pathway databases

ReactomeiREACT_1100. Golgi Cisternae Pericentriolar Stack Reorganization.

Miscellaneous databases

ChiTaRSiGOLGA2. human.
GeneWikiiGOLGA2.
GenomeRNAii2801.
NextBioi11041.
PROiQ08379.
SOURCEiSearch...

Gene expression databases

BgeeiQ08379.
CleanExiHS_GOLGA2.
ExpressionAtlasiQ08379. baseline and differential.
GenevestigatoriQ08379.

Family and domain databases

InterProiIPR024858. Golgin_A.
[Graphical view]
PANTHERiPTHR10881. PTHR10881. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of two human autoantigens: unique cDNAs encoding 95- and 160-kD proteins of a putative family in the Golgi complex."
    Fritzler M.J., Hamel J.C., Ochs R.L., Chan E.K.L.
    J. Exp. Med. 178:49-62(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Liver.
  2. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain and Colon adenocarcinoma.
  4. "The Golgi matrix protein GM130: a specific interacting partner of the small GTPase rab1b."
    Weide T., Bayer M., Koester M., Siebrasse J.-P., Peters R., Barnekow A.
    EMBO Rep. 2:336-341(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-1002 (ISOFORM 1), INTERACTION WITH RAB1B.
    Tissue: Placenta.
  5. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 528-1002 (ISOFORM 1).
  6. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
    Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
    Nat. Biotechnol. 24:1285-1292(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "ZFPL1, a novel ring finger protein required for cis-Golgi integrity and efficient ER-to-Golgi transport."
    Chiu C.-F., Ghanekar Y., Frost L., Diao A., Morrison D., McKenzie E., Lowe M.
    EMBO J. 27:934-947(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ZFPL1.
  8. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37 AND SER-438, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-953, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-273; SER-690 AND SER-937, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiGOGA2_HUMAN
AccessioniPrimary (citable) accession number: Q08379
Secondary accession number(s): Q6GRM9, Q9BRB0, Q9NYF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 20, 2010
Last modified: March 4, 2015
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.