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UniProtKB/Swiss-Prot Q08378 (GOGA3_HUMAN)
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January 19, 2010.
Version 93.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Golgin subfamily A member 3 Alternative name(s): Golgin-160 Golgi complex-associated protein of 170 kDa Short name=GCP170 | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) [Complete proteome] | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 1498 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Golgi auto-antigen; probably involved in maintaining Golgi structure. |
| Subunit structure | Homodimer. Interacts with GOLGA7. Isoform 1 interacts with GOPC while isoform 3 does not. Ref.6 Ref.7 Ref.8 |
| Subcellular location | Cytoplasm. Golgi apparatus › Golgi stack membrane; Peripheral membrane protein Ref.6 Ref.7 Ref.8 Ref.1 Ref.2. |
| Tissue specificity | Expressed in all tissues tested. Expressed in liver, testis, lung, heart, salivary gland and kidney. Ref.8 |
| Domain | Extended rod-like protein with coiled-coil domains. |
| Post-translational modification | Cleaved by caspases in apoptotic cells. |
| Sequence caution | The sequence AAH60826.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence. The sequence BAA23661.1 differs from that shown. Reason: Frameshift at position 1378. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm Golgi apparatus Membrane |
| Coding sequence diversity | Alternative splicing Polymorphism |
| Domain | Coiled coil |
| PTM | Acetylation Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | intra-Golgi vesicle-mediated transport Ref.5 Non-traceable author statement. Source: UniProtKB |
| Cellular component | Golgi membrane Ref.1 Traceable author statement. Source: ProtInc Golgi transport complex Ref.5Inferred from direct assay. Source: UniProtKB extrinsic to membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | protein binding Inferred from physical interaction. Source: IntAct transporter activity Ref.5Non-traceable author statement. Source: UniProtKB |
| Complete GO annotation... | |
Alternative products
| This entry describes 3 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q08378-1) Also known as: Golgin-160B; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q08378-2) The sequence of this isoform differs from the canonical sequence as follows: 1382-1390: RKEPKGEAS → VLRPASLPG 1391-1498: Missing. | ||||||
| Note: No experimental confirmation available. May be due to an intron retention. | ||||||
| Isoform 3 (identifier: Q08378-4) The sequence of this isoform differs from the canonical sequence as follows: 1090-1134: LQESRGFRKK...GQSNAALREH → VRPGHLLWRQ...SVATFGVATF 1135-1498: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1498 | 1498 | Golgin subfamily A member 3 | PRO_0000190057 | |||||
Regions | |||||||||
| Region | 121 – 141 | 21 | Interaction with GOPC | ||||||
| Region | 172 – 257 | 86 | Golgi-targeting domain | ||||||
| Coiled coil | 394 – 1459 | 1066 | Potential | ||||||
| Compositional bias | 20 – 95 | 76 | Pro-rich | ||||||
| Compositional bias | 538 – 744 | 207 | Gln-rich | ||||||
| Compositional bias | 1231 – 1369 | 139 | Gln-rich | ||||||
Sites | |||||||||
| Site | 59 – 60 | 2 | Cleavage; by caspase-2 | ||||||
| Site | 139 – 140 | 2 | Cleavage; by caspase-3 | ||||||
| Site | 311 – 312 | 2 | Cleavage; by caspase-7 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1 | 1 | N-acetylmethionine Ref.11 | ||||||
| Modified residue | 57 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 389 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 392 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 393 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 395 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 399 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 528 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 983 | 1 | Phosphoserine Ref.12 | ||||||
Natural variations | |||||||||
| Alternative sequence | 1090 – 1134 | 45 | LQESR…ALREH → VRPGHLLWRQRGAGHVSPGH AATRETRRTKLHRVPSVATF GVATF in isoform 3. | VSP_038000 | |||||
| Alternative sequence | 1135 – 1498 | 364 | Missing in isoform 3. | VSP_038001 | |||||
| Alternative sequence | 1382 – 1390 | 9 | RKEPKGEAS → VLRPASLPG in isoform 2. | VSP_007728 | |||||
| Alternative sequence | 1391 – 1498 | 108 | Missing in isoform 2. | VSP_007729 | |||||
| Natural variant | 70 | 1 | G → E: dbSNP rs2291256. | VAR_020153 | |||||
| Natural variant | 264 | 1 | P → L: dbSNP rs3741486. | VAR_021901 | |||||
| Natural variant | 1185 | 1 | K → R: dbSNP rs2291260. | VAR_020154 | |||||
Experimental info | |||||||||
| Mutagenesis | 59 | 1 | D → A: Abolishes cleavage by caspase-2. Ref.2 | ||||||
| Mutagenesis | 121 | 1 | L → A: Loss of interaction with GOPC; when associated with A-128 and A-135. Ref.8 | ||||||
| Mutagenesis | 128 | 1 | L → A: Loss of interaction with GOPC; when associated with A-121 and A-135. Ref.8 | ||||||
| Mutagenesis | 135 | 1 | L → A: Loss of interaction with GOPC; when associated with A-121 and A-128. Ref.8 | ||||||
| Mutagenesis | 139 | 1 | D → A: Abolishes cleavage by caspase-3. Ref.2 | ||||||
| Mutagenesis | 311 | 1 | D → A: Abolishes cleavage by caspase-7. Ref.2 | ||||||
| Sequence conflict | 159 | 1 | E → K in AAL93149. Ref.2 | ||||||
| Sequence conflict | 609 | 1 | L → I in BAA23661. Ref.1 | ||||||
| Sequence conflict | 746 | 1 | H → R in AAL93149. Ref.2 | ||||||
| Sequence conflict | 785 | 1 | A → V in AAA35921. Ref.4 | ||||||
| Sequence conflict | 932 | 1 | M → V in AAL93149. Ref.2 | ||||||
| Sequence conflict | 1017 | 1 | K → R in AAL93149. Ref.2 | ||||||
| Sequence conflict | 1281 | 1 | V → A in AAL93149. Ref.2 | ||||||
| Sequence conflict | 1315 | 1 | R → W in AAL93149. Ref.2 | ||||||
| Sequence conflict | 1443 | 1 | Q → R in AAL93149. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular characterization of GCP170, a 170-kDa protein associated with the cytoplasmic face of the Golgi membrane." Misumi Y., Sohda M., Yano A., Fujiwara T., Ikehara Y. J. Biol. Chem. 272:23851-23858(1997) [PubMed: 9295333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PHOSPHORYLATION, SUBCELLULAR LOCATION. Tissue: Pancreatic carcinoma. |
| [2] | "Caspase-2 is localized at the Golgi complex and cleaves golgin-160 during apoptosis." Mancini M., Machamer C.E., Roy S., Nicholson D.W., Thornberry N.A., Casciola-Rosen L.A., Rosen A. J. Cell Biol. 149:603-612(2000) [PubMed: 10791974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), MUTAGENESIS OF ASP-59; ASP-139 AND ASP-311, SUBCELLULAR LOCATION, PHOSPHORYLATION, CLEAVAGE BY CASPASES. |
| [3] | "Molecular characterization of Mea-2/golgin-160/GCP170 gene encoding a Golgi membrane associated protein." Kondo M., Matsukuma S., Hirose F., Matsuda M., Yoshihara M., Misumi Y., Aida M., Ikehara M., Sutou S. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-840 (ISOFORMS 1/2/3). Tissue: Placenta. |
| [5] | "Molecular characterization of two human autoantigens: unique cDNAs encoding 95- and 160-kD proteins of a putative family in the Golgi complex." Fritzler M.J., Hamel J.C., Ochs R.L., Chan E.K.L. J. Exp. Med. 178:49-62(1993) [PubMed: 8315394] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 780-1348. Tissue: Liver. |
| [6] | "The NH2-terminal domain of Golgin-160 contains both Golgi and nuclear targeting information." Hicks S.W., Machamer C.E. J. Biol. Chem. 277:35833-35839(2002) [PubMed: 12130652] [Abstract] Cited for: DIMERIZATION, SUBCELLULAR LOCATION. |
| [7] | "Identification and characterization of GCP16, a novel acylated Golgi protein that interacts with GCP170." Ohta E., Misumi Y., Sohda M., Fujiwara T., Yano A., Ikehara Y. J. Biol. Chem. 278:51957-51967(2003) [PubMed: 14522980] [Abstract] Cited for: INTERACTION WITH GOLGA7, SUBCELLULAR LOCATION. |
| [8] | "Isoform-specific interaction of golgin-160 with the Golgi-associated protein PIST." Hicks S.W., Machamer C.E. J. Biol. Chem. 280:28944-28951(2005) [PubMed: 15951434] [Abstract] Cited for: INTERACTION WITH GOPC, TISSUE SPECIFICITY, ALTERNATIVE SPLICING (ISOFORM 3), SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-121; LEU-128 AND LEU-135. |
| [9] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-389; SER-392; SER-393; SER-395; SER-399 AND SER-528, MASS SPECTROMETRY. Tissue: Epithelium. |
| [10] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [11] | "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, MASS SPECTROMETRY. |
| [12] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-983, MASS SPECTROMETRY. Tissue: T-cell. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | D63997 mRNA. Translation: BAA23661.1. Frameshift. AF485338 mRNA. Translation: AAL93149.1. AB027133 mRNA. Translation: BAB71953.1. BC060826 mRNA. Translation: AAH60826.1. Sequence problems. BC142658 mRNA. Translation: AAI42659.1. BC146675 mRNA. Translation: AAI46676.1. L06148 mRNA. Translation: AAA35921.1. Different initiation. |
| IPI | IPI00305267. IPI00333419. IPI00944623. |
| PIR | JH0820. |
| RefSeq | NP_005886.2. |
| UniGene | Hs.507333 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q08378. 2 interactions. |
| STRING | Q08378. |
PTM databases | |
| PhosphoSite | Q08378. |
Proteomic databases | |
| PRIDE | Q08378. |
Genome annotation databases | |
| Ensembl | ENST00000204726; ENSP00000204726; ENSG00000090615; Homo sapiens. [Genome view] |
| GeneID | 2802. |
| KEGG | hsa:2802. |
| UCSC | uc001ukz.1. human. uc001ula.1. human. |
Organism-specific databases | |
| CTD | 2802. |
| GeneCards | GC12M131855. |
| H-InvDB | HIX0036664. |
| HGNC | HGNC:4426. GOLGA3. |
| MIM | 602581. gene. |
| PharmGKB | PA28807. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG16855. |
| HOGENOM | HBG402826. |
| HOVERGEN | Q08378. |
| InParanoid | Q08378. |
| OMA | AGHNRRH. |
| OrthoDB | EOG9G7FJW. |
| PhylomeDB | Q08378. |
Gene expression databases | |
| ArrayExpress | Q08378. |
| Bgee | Q08378. |
| CleanEx | HS_GOLGA3. |
| Genevestigator | Q08378. |
| GermOnline | ENSG00000090615. Homo sapiens. |
Family and domain databases | |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 11045. |
| PMAP-CutDB | Q08378. |
| SOURCE | Search... |
Entry information
| Entry name | GOGA3_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q08378 Secondary accession number(s): A5PKX6  Q8WZA3 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 12 Human chromosome 12: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
